{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:58:04Z","timestamp":1762865884022,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T00:00:00Z","timestamp":1319155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH\/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The \u201cblue-shift\u201d in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.<\/jats:p>","DOI":"10.3390\/s111009951","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T11:37:59Z","timestamp":1319197079000},"page":"9951-9972","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications"],"prefix":"10.3390","volume":"11","author":[{"given":"Alexandra","family":"Mansur","sequence":"first","affiliation":[{"name":"Department of Metallurgical and Materials Engineering, School of Engineering, Federal University of Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Pampulha, Belo Horizonte\/MG, 31.270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herman","family":"Mansur","sequence":"additional","affiliation":[{"name":"Department of Metallurgical and Materials Engineering, School of Engineering, Federal University of Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Pampulha, Belo Horizonte\/MG, 31.270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Physics, Federal University of Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Pampulha, Belo Horizonte\/MG, 31.270-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2011,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4855","DOI":"10.3390\/s100504855","article-title":"A comprehensive review of glucose biosensors based on nanostructured metal-oxides","volume":"10","author":"Rahman","year":"2010","journal-title":"Sensors"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4034","DOI":"10.3390\/s90604034","article-title":"Nanomaterials\u2014Acetylcholinesterase enzyme matrices for organophosphorus pesticides electrochemical sensors: A review","volume":"9","author":"Periasamy","year":"2009","journal-title":"Sensors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3845","DOI":"10.1016\/j.bios.2011.02.044","article-title":"Highly stable and sensitive glucose biosensor based on covalently assembled high density Au nanostructures","volume":"26","author":"Si","year":"2011","journal-title":"Biosens. 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