{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:49:34Z","timestamp":1790768974598,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,11]],"date-time":"2018-04-11T00:00:00Z","timestamp":1523404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)\/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2\u2013200 ng\/mL detection range which can be used for on-site detection of feedstuffs.<\/jats:p>","DOI":"10.3390\/s18041161","type":"journal-article","created":{"date-parts":[[2018,4,11]],"date-time":"2018-04-11T12:16:50Z","timestamp":1523449010000},"page":"1161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Label-Free QCM Immunosensor for the Detection of Ochratoxin A"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5659-5806","authenticated-orcid":false,"given":"\u015eerife","family":"Pirin\u00e7ci","sequence":"first","affiliation":[{"name":"T\u00dcB\u0130TAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey"},{"name":"Department of Medical Genetics and Molecular Biology, Kocaeli University, Umuttepe, Kocaeli 41100, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"Ertekin","sequence":"additional","affiliation":[{"name":"T\u00dcB\u0130TAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duygu","family":"Laguna","sequence":"additional","affiliation":[{"name":"T\u00dcB\u0130TAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fehime","family":"\u00d6zen","sequence":"additional","affiliation":[{"name":"T\u00dcB\u0130TAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zafer","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Physics, Gebze Technical University, Gebze, Kocaeli 41400, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"T\u00dcB\u0130TAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3109\/10408444.2010.529103","article-title":"Human ochratoxin A biomarkers\u2014From exposure to effect","volume":"41","author":"Duarte","year":"2011","journal-title":"Crit. 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