{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T01:01:05Z","timestamp":1775091665168,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2019,7,20]],"date-time":"2019-07-20T00:00:00Z","timestamp":1563580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MSIT(Ministry of Science and ICT), Korea, under the ITRC(Information Technology Research Center) supervised by the IITP(Institute for Information &amp; Communications Technology Planning &amp; Evaluation)","award":["IITP-2019-2018-0-01798"],"award-info":[{"award-number":["IITP-2019-2018-0-01798"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurement and heating system in non-contact with the surface of LOD, which is a prerequisite to successful realization of DNA amplification especially with a rotatable disc. This study presents a Lab-on-a-disc (LOD)-based automatic loop-mediated isothermal amplification (LAMP) system, where a thermochromic coating (&lt;~420 \u00b5m) was used to distantly measure the chamber\u2019s temperature and a micro graphite film was integrated into the chamber to remotely absorb laser beam with super high efficiency. We used a deep learning network to more consistently analyze the product of LAMP than we could with the naked eye. Consequently, both temperature heating and measurement were carried out without a physical contact with the surface of LOD. The experimental results show that the proposed approach, which no previous work has attempted, was highly effective in realizing LAMP in LOD.<\/jats:p>","DOI":"10.3390\/s19143207","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T02:55:37Z","timestamp":1563764137000},"page":"3207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform"],"prefix":"10.3390","volume":"19","author":[{"given":"Da Ye Seul","family":"Lim","sequence":"first","affiliation":[{"name":"College of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-746, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moo-Jung","family":"Seo","sequence":"additional","affiliation":[{"name":"College of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-746, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Chern","family":"Yoo","sequence":"additional","affiliation":[{"name":"College of Information and Communication Engineering, Sungkyunkwan University, Suwon, Gyeonggi-Do 440-746, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"644","DOI":"10.3389\/fmicb.2016.00644","article-title":"Advances in Molecular Serotyping and Subtyping of Escherichia coli","volume":"7","author":"Fratamico","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.ss6710a1","article-title":"Surveillance for foodborne disease outbreaks\u2014United States, 2009\u20132015","volume":"67","author":"Manikonda","year":"2018","journal-title":"MMWR Surveill. Summ."},{"key":"ref_3","unstructured":"(2019, May 09). Outbreaks of Foodborne Illness. Available online: https:\/\/www.fda.gov\/food\/recalls-outbreaks-emergencies\/outbreaks-foodborne-illness."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Oseev, A., Lucklum, R., Zubtsov, M., Schmidt, M.P., Mukhin, N.V., and Hirsch, S. (2017). SAW-Based Phononic Crystal Microfluidic Sensor\u2014Microscale Realization of Velocimetry Approaches for Integrated Analytical Platform Applications. Sensors, 17.","DOI":"10.3390\/s17102187"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/nature05064","article-title":"Microfluidic diagnostic technologies for global public health","volume":"442","author":"Yager","year":"2006","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3390\/diagnostics3010105","article-title":"Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation","volume":"3","author":"Packard","year":"2013","journal-title":"Diagnostics"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/j.snb.2008.11.035","article-title":"Novel polydimethylsiloxane (PDMS) based microchannel fabrication method for lab-on-a-chip application","volume":"137","author":"Yu","year":"2009","journal-title":"Sens. Actuators B Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1021\/ac902683t","article-title":"High-Performance Single Cell Genetic Analysis Using Microfluidic Emulsion Generator Arrays","volume":"82","author":"Zeng","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s10404-018-2112-y","article-title":"A positive pressure-driven PDMS pump for fluid handling in microfluidic chips","volume":"22","author":"Liu","year":"2018","journal-title":"Microfluid. Nanofluid."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3841","DOI":"10.1021\/ac403971h","article-title":"Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens","volume":"86","author":"Kim","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1039\/C4LC00947A","article-title":"From cellular lysis to microarray detection, an integrated thermoplastic elastomer (TPE) point of care Lab on a Disc","volume":"15","author":"Roy","year":"2015","journal-title":"Lab Chip"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Joseph, K., Ibrahim, F., Cho, J., Thio, T.H.G., Al-Faqheri, W., and Madou, M. (2015). Design and Development of Micro-Power Generating Device for Biomedical Applications of Lab-on-a-Disc. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136519"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.bios.2016.05.023","article-title":"Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano- and micro-sized magnetic beads","volume":"85","author":"Uddin","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.aca.2013.04.016","article-title":"At-line bioprocess monitoring by immunoassay with rotationally controlled serial siphoning and integrated supercritical angle fluorescence optics","volume":"781","author":"Nwankire","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1021\/ac3036734","article-title":"Lab-on-a-Disc for Simultaneous Determination of Nutrients in Water","volume":"85","author":"Hwang","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3923\/ajft.2011.87.102","article-title":"Methods for Rapid Detection of Foodborne Pathogens: An Overview","volume":"6","author":"Mandal","year":"2011","journal-title":"Am. J. Food Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.snb.2015.10.116","article-title":"A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection","volume":"227","author":"Sayad","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5376","DOI":"10.3390\/s150305376","article-title":"A Portable Automatic Endpoint Detection System for Amplicons of Loop Mediated Isothermal Amplification on Microfluidic Compact Disk Platform","volume":"15","author":"Uddin","year":"2015","journal-title":"Sensors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4076","DOI":"10.1039\/C4LC00443D","article-title":"An integrated CMOS quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics","volume":"14","author":"Norian","year":"2014","journal-title":"Lab Chip"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27954","DOI":"10.3390\/s151127954","article-title":"Centrifugal Microfluidic System for Nucleic Acid Amplification and Detection","volume":"15","author":"Miao","year":"2015","journal-title":"Sensors"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1146\/annurev.mi.45.100191.001033","article-title":"Polymerase chain reaction: Applications in environmental microbiology","volume":"45","author":"Steffan","year":"1991","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/sj.embor.7400200","article-title":"Helicase-dependent isothermal DNA amplification","volume":"5","author":"Vincent","year":"2004","journal-title":"EMBO Rep."},{"key":"ref_23","first-page":"1441","article-title":"Isothermal multiple displacement amplification: A methodical approach enhancing molecular routine diagnostics of microcarcinomas and small biopsies","volume":"7","author":"Mairinger","year":"2014","journal-title":"Onco Targets Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7540","DOI":"10.1039\/C5AN01632K","article-title":"Isothermal strand displacement amplification (iSDA): A rapid and sensitive method of nucleic acid amplification for point-of-care diagnosis","volume":"140","author":"Toley","year":"2015","journal-title":"Analyst"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8541","DOI":"10.1021\/ac3020609","article-title":"Multifunctional Sample Preparation Kit and On-Chip Quantitative Nucleic Acid Sequence-Based Amplification Tests for Microbial Detection","volume":"84","author":"Zhao","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1002\/smll.201303325","article-title":"On-Chip Detection of Rolling Circle Amplified DNA Molecules from Bacillus Globigii Spores and Vibrio Cholerae","volume":"10","author":"Rizzi","year":"2014","journal-title":"Small"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1039\/C4AN01683A","article-title":"Real-time microfluidic recombinase polymerase amplification for the toxin B gene of Clostridium difficile on a SlipChip platform","volume":"140","author":"Tsaloglou","year":"2015","journal-title":"Analyst"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1373\/clinchem.2005.053694","article-title":"Novel Isothermal, Linear Nucleic Acid Amplification Systems for Highly Multiplexed Applications","volume":"51","author":"Kurn","year":"2005","journal-title":"J. Clin. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Duan, Y.B., Ge, C.Y., Zhang, X.K., Wang, J.X., and Zhou, M.G. (2014). Development and Evaluation of a Novel and Rapid Detection Assay for Botrytis cinerea Based on Loop-Mediated Isothermal Amplification. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111094"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/bbrc.2001.5921","article-title":"Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation","volume":"289","author":"Mori","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2144\/000113072","article-title":"Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue","volume":"46","author":"Goto","year":"2009","journal-title":"Biotechniques"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"189","DOI":"10.2144\/000114275","article-title":"Shining a light on LAMP assays\u2019 A comparison of LAMP visualization methods including the novel use of berberine","volume":"58","author":"Fischbach","year":"2015","journal-title":"Biotech"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1007\/s12010-015-1546-x","article-title":"Automated Centrifugal-Microfluidic Platform for DNA Purification Using Laser Burst Valve and Coriolis Effect","volume":"175","author":"Choi","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_35","first-page":"54","article-title":"Loop-Mediated Isothermal Amplification Using a Lab-on-a-Disc Device with Thin-film Phase Change Material","volume":"186","author":"Ko","year":"2018","journal-title":"Sensors"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"McLinden, T., Sargeant, J.M., Thomas, M.K., Papadopoulos, A., and Fazil, A. (2014). Component costs of foodborne illness: A scoping review. BMC Public Health, 14.","DOI":"10.1186\/1471-2458-14-509"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/1751-7915.12359","article-title":"Recent and emerging innovations in Salmonella detection: A food and environmental perspective","volume":"9","author":"Bell","year":"2016","journal-title":"Microb. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"655","DOI":"10.5851\/kosfa.2013.33.5.655","article-title":"Rapid and Sensitive Detection of Salmonella Spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample","volume":"33","author":"Cho","year":"2013","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"ref_39","unstructured":"Fu, M.F.F. (2016). Advanced High Strength Natural Fibre Composites in Construction, ELSEVIER. [1st ed.]."},{"key":"ref_40","unstructured":"Rachmadi, R.F., and Purnama, I. (2015). Vehicle color recognition using convolutional neural network. arXiv."},{"key":"ref_41","first-page":"1513","article-title":"Colorimetric Characterization of Color Image Sensors Based on Convolutional Neural Network Modeling","volume":"31","author":"Wang","year":"2019","journal-title":"Sens. Mater."},{"key":"ref_42","unstructured":"Xia, X., Xia, C., Bing, X., and Nan, X. (2017, January 2\u20134). Inception-v3 for flower classification. Proceedings of the 2017 2nd International Conference on Image, Vision and Computing (ICIVC), Chengdu, China."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Szegedy, C., Vanhoucke, V., Ioffe, S., Shlens, J., and Wojna, Z. (2016, January 27\u201330). Rethinking the inception architecture for computer vision. Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition, Las Vegas, NV, USA.","DOI":"10.1109\/CVPR.2016.308"},{"key":"ref_44","unstructured":"(2019, July 05). Review: Inception-v3\u20141st Runner Up (Image Classification) in ILSVRC 2015. Available online: https:\/\/medium.com\/@sh.tsang\/review-inception-v3-1st-runner-up-image-classification-in-ilsvrc-2015-17915421f77c."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6495","DOI":"10.1128\/AEM.00520-11","article-title":"Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for the detection of Salmonella spp. in produce","volume":"77","author":"Zhang","year":"2011","journal-title":"J. Appl. Enciron. Microbiol."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/14\/3207\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:07:57Z","timestamp":1760188077000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/14\/3207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,20]]},"references-count":45,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2019,7]]}},"alternative-id":["s19143207"],"URL":"https:\/\/doi.org\/10.3390\/s19143207","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,20]]}}}