{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:57:58Z","timestamp":1790708278621,"version":"4.1.0"},"reference-count":20,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T00:00:00Z","timestamp":1571184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>One of the most common causes for larynx paralysis is the injury of the recurrent laryngeal nerve which, among others, causes the paralysis of the posterior cricoarytenoideus muscle (PCA). Electrical stimulation of PCA offers an approach to retaining the function of the paralyzed larynx muscle. The study aim was to test the applicability of an intramuscular multichannel array electrode as a measuring electrode for myoelectrical potentials and as a possible electrode for stimulation, e.g., posterior cricoarytenoideus muscle stimulation. For this purpose, two different kinds of electrodes were compared. 42 intramuscular multichannel array electrodes and 11 supramysial multichannel electrodes were implanted into the triceps brachii muscle of rats. The triceps brachii muscle of rats is suitable to serve as a substitute muscle for the human PCA muscle in an in vivo animal model. It has the same striated muscle cells, is of comparable size, and fundamentally serves a similar function to the human PCA muscle during normal respiration. Walking and breathing are circular functions that cause minimal muscle fatigue when carried out steadily. In total, the myoelectrical activity of 6703 steps could be recorded, allowing a comparison and statistical analysis of the EMG amplitudes and EMG activation patterns. Small differences can be detected between the EMG signals of both electrode types which, however, can be explained physiologically. Both electrode types reveal the basic characteristics of the triceps brachii muscle activity, namely the muscle contraction strength and the coordination pattern. This indicates that the intramuscular electrode may be applied for a detailed analysis of the human larynx.<\/jats:p>","DOI":"10.3390\/s19204477","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T04:46:06Z","timestamp":1571287566000},"page":"4477","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison between Intramuscular Multichannel Electrodes and Supramysial Multichannel Electrodes via EMG Measurements for Potential Use as Larynx Stimulation Electrodes: In Vivo Animal Analysis"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6568-6926","authenticated-orcid":false,"given":"Bernd","family":"Faenger","sequence":"first","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"},{"name":"Institute of Diagnostic and Interventional Radiology, Department of Experimental Radiology, Research Center Lobeda, Jena University Hospital, Friedrich-Schiller-University, 07747 Jena, Germany"},{"name":"Institute of Pathology, Neuropathology Section, Jena University Hospital, Friedrich-Schiller-University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus P.","family":"Schumann","sequence":"additional","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Anders","sequence":"additional","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Arnold","sequence":"additional","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"},{"name":"Institute of Zoology and Evolutionary Research, Friedrich-Schiller-University Jena, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Grassme","sequence":"additional","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"},{"name":"Employer\u2019s Liability Insurance Association for Food and the Hospitality Industry (Berufsgenossenschaft Nahrungsmittel und Gastgewerbe), Department for Prevention, branch office Erfurt, 99097 Erfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9671-0784","authenticated-orcid":false,"given":"Orlando","family":"Guntinas-Lichius","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Christoph","family":"Scholle","sequence":"additional","affiliation":[{"name":"Division for Motor Research, Pathophysiology and Biomechanics, Department for Trauma-, Hand- and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4203","DOI":"10.1121\/1.4831428","article-title":"The role of the posterior cricoarytenoid muscle in phonation","volume":"134","author":"Berry","year":"2013","journal-title":"J. Acoust. Soc. Am."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0892-1997(05)80310-8","article-title":"Laryngeal pacing as a treatment for vocal fold paralysis","volume":"8","author":"Goldfarb","year":"1994","journal-title":"J. Voice"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1177\/000348949610500904","article-title":"Electrical pacing of the paralyzed human larynx","volume":"105","author":"Zealear","year":"1996","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1152\/jappl.1990.68.4.1739","article-title":"Intramuscular and esophageal electrode recordings of posterior cricoarytenoid activity in normal subjects","volume":"68","author":"Kuna","year":"1990","journal-title":"J. Appl. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1097\/00005537-199701000-00024","article-title":"Major patterns of laryngeal electromyography and their clinical application","volume":"107","author":"Yin","year":"1997","journal-title":"Laryngoscope"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1007\/s00405-012-2036-1","article-title":"Laryngeal electromyography: A proposal for guidelines of the european laryngological society","volume":"269","author":"Volk","year":"2012","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/lary.25512","article-title":"Development of a novel larynx pacemaker multichannel array electrode: In vivo animal analysis","volume":"126","author":"Faenger","year":"2016","journal-title":"Laryngoscope"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s00405-016-4202-3","article-title":"Method to test the long-term stability of functional electrical stimulation via multichannel electrodes (e.G., applicable for laryngeal pacing) and to define best points for stimulation: In vivo animal analysis","volume":"274","author":"Faenger","year":"2017","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jvoice.2007.03.004","article-title":"Analysis of normal and denerved laryngeal vocalization in guinea pigs (cavia porcellus)","volume":"23","year":"2009","journal-title":"J. Voice"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0165-0270(00)00170-9","article-title":"Surface emg-recordings using a miniaturised matrix electrode: A new technique for small animals","volume":"97","author":"Biedermann","year":"2000","journal-title":"J. Neurosci. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/1050-6411(94)00014-X","article-title":"Normalization of surface emg amplitude from the upper trapezius muscle in ergonomic studies\u2014A review","volume":"5","author":"Mathiassen","year":"1995","journal-title":"J. Electromyogr Kinesiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bortz, J., Lienert, G.A., and Boehnke, K. (2000). Verteilungsfreie Methoden in der Biostatistik, Springer.","DOI":"10.1007\/978-3-662-10786-7"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s002210100685","article-title":"Spatiotemporal surface emg characteristics from rat triceps brachii muscle during treadmill locomotion indicate selective recruitment of functionally distinct muscle regions","volume":"138","author":"Scholle","year":"2001","journal-title":"Exp. Brain Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/jmor.1051460202","article-title":"Muscle activity in rat locomotion: Movement analysis and electromyography of the flexors and extensors of the elbow","volume":"146","author":"Cohen","year":"1975","journal-title":"J. Morphol."},{"key":"ref_15","unstructured":"Blickhan, R. (2001). Threedimensional evaluation of multichannel surface emg for dynamic movements by temporal filtering and investigation of intramuscular coordination in rats during treadmill locomotion. Motion Systems, Shaker Verlag."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/S1388-2457(02)00143-8","article-title":"Multi-channel emg of the m. Triceps brachii in rats during treadmill locomotion","volume":"113","author":"Schumann","year":"2002","journal-title":"Clin. Neurophysiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.jelekin.2008.04.013","article-title":"Heterogeneity of muscle activation in relation to force direction: A multi-channel surface electromyography study on the triceps surae muscle","volume":"19","author":"Staudenmann","year":"2008","journal-title":"J. Electromyogr Kinesiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1177\/000348940211100605","article-title":"Electrically stimulated glottal opening combined with adductor muscle botox blockade restores both ventilation and voice in a patient with bilateral laryngeal paralysis","volume":"111","author":"Zealear","year":"2002","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1097\/00005537-200307000-00010","article-title":"Reanimation of the paralyzed human larynx with an implantable electrical stimulation device","volume":"113","author":"Zealear","year":"2003","journal-title":"Laryngoscope"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1002\/lary.20587","article-title":"Rehabilitation of bilaterally paralyzed canine larynx with implantable stimulator","volume":"119","author":"Zealear","year":"2009","journal-title":"Laryngoscope"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/20\/4477\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:26:43Z","timestamp":1760189203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/19\/20\/4477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,16]]},"references-count":20,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["s19204477"],"URL":"https:\/\/doi.org\/10.3390\/s19204477","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,16]]}}}