{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T02:25:15Z","timestamp":1780539915750,"version":"3.54.1"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["18-33-20111"],"award-info":[{"award-number":["18-33-20111"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Russian Scientific Foundation","award":["20-73-10186"],"award-info":[{"award-number":["20-73-10186"]}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Bis(styryl) dye 1 bearing N-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg2+ via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 \u00b1 0.15 \u00b5M). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV\/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500\u2013600 nm) to red (600\u2013730 nm) fluorescence intensity ratio decreased from 4.4 \u00b1 0.2 to 1.43 \u00b1 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg2+ concentration in the 37 nM\u20131 \u03bcM range.<\/jats:p>","DOI":"10.3390\/s21020470","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T11:36:11Z","timestamp":1610364971000},"page":"470","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor"],"prefix":"10.3390","volume":"21","author":[{"given":"Pavel","family":"Panchenko","sequence":"first","affiliation":[{"name":"Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia"},{"name":"Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasija","family":"Efremenko","sequence":"additional","affiliation":[{"name":"Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1596-9506","authenticated-orcid":false,"given":"Alexey","family":"Feofanov","sequence":"additional","affiliation":[{"name":"Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-594X","authenticated-orcid":false,"given":"Mariya","family":"Ustimova","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Fedorov","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences (INEOS RAS), 119991 Moscow, Russia"},{"name":"Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1039\/b916287a","article-title":"Fluorescent chemosensors for Zn2+","volume":"39","author":"Xu","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ica.2011.09.011","article-title":"Recent progress on fluorescent chemosensors for metal ions","volume":"381","author":"Jeong","year":"2012","journal-title":"Inorganica Chim. 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