{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:21:42Z","timestamp":1790882502463,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009409","name":"Russian Science Support Foundation","doi-asserted-by":"publisher","award":["17-73-20024"],"award-info":[{"award-number":["17-73-20024"]}],"id":[{"id":"10.13039\/100009409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Electropolymerized redox polymers offer broad opportunities in detection of biospecific interactions of DNA. In this work, Azure A was electrochemically polymerized by multiple cycling of the potential in phosphate buffer saturated with chloroform and applied for discrimination of the DNA damage. The influence of organic solvent on electrochemical properties of the coating was quantified and conditions for implementation of DNA in the growing polymer film were assessed using cyclic voltammetry, quartz crystal microbalance, and electrochemical impedance spectroscopy. As shown, both chloroform and DNA affected the morphology of the polymer surface and electropolymerization efficiency. The electrochemical DNA sensor developed made it possible to distinguish native and thermally and chemically damaged DNA by changes in the charge transfer resistance and capacitance.<\/jats:p>","DOI":"10.3390\/s21092949","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T21:25:56Z","timestamp":1619126756000},"page":"2949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Electrochemical DNA Sensor Based on Poly(Azure A) Obtained from the Buffer Saturated with Chloroform"],"prefix":"10.3390","volume":"21","author":[{"given":"Anna","family":"Porfireva","sequence":"first","affiliation":[{"name":"A.M. Butlerov\u2019 Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kseniya","family":"Plastinina","sequence":"additional","affiliation":[{"name":"A.M. Butlerov\u2019 Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Evtugyn","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center of Analytical Microscopy of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurii","family":"Kuzin","sequence":"additional","affiliation":[{"name":"A.M. Butlerov\u2019 Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4694-7836","authenticated-orcid":false,"given":"Gennady","family":"Evtugyn","sequence":"additional","affiliation":[{"name":"A.M. Butlerov\u2019 Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia"},{"name":"Analytical Chemistry Department of Chemical Technology Institute of Ural Federal University, 19 Mira Street, 620002 Ekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nejadmansouri, M., Majdinasab, M., Nunes, G.S., and Marty, J.L. (2021). An overview of optical and electrochemical sensors and biosensors for analysis of antioxidants in food during the last 5 years. Sensors, 21.","DOI":"10.3390\/s21041176"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"037503","DOI":"10.1149\/1945-7111\/abe8b6","article-title":"Review\u2014recent advances in microfabrication, design and applications of amperometric sensors and biosensors","volume":"168","author":"Baracu","year":"2021","journal-title":"J. Electrochem. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1080\/10408347.2019.1615406","article-title":"A review: New trends in electrode systems for sensitive drug and biomolecule analysis","volume":"50","author":"Ozcelikay","year":"2020","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2018.06.014","article-title":"Portable analytical platforms for forensic chemistry: A review","volume":"1034","author":"Cardoso","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.aca.2016.12.035","article-title":"How cutting-edge technologies impact the design of electrochemical (bio)sensors for environmental analysis. A review","volume":"59","author":"Arduini","year":"2017","journal-title":"Anal. Chem. Acta"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Coluccio, M.L., Pullano, S.A., Vismara, M.F.M., Copped\u00e8, N., Perozziello, G., Candeloro, P., Gentile, F., and Malara, N. (2020). Emerging designs of electronic devices in biomedicine. Micromachines, 11.","DOI":"10.3390\/mi11020123"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1021\/acs.analchem.6b04630","article-title":"Point-of-care diagnostics: Recent developments in a connected age","volume":"89","author":"Nayak","year":"2017","journal-title":"Anal. Chem."},{"key":"ref_8","first-page":"105","article-title":"Interferences in immunoassay","volume":"25","author":"Tate","year":"2004","journal-title":"Clin. Biochem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1039\/C9AN02047K","article-title":"Electrochemical biosensors based on antibody, nucleic acid and enzyme functionalized graphene for the detection of disease-related biomolecules","volume":"145","author":"Wang","year":"2020","journal-title":"Analyst"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Asal, M., \u00d6zen, \u00d6., Sahinler, M., and Polato\u011flu, I. (2018). Recent developments in enzyme, DNA and immuno-based biosensors. Sensors, 18.","DOI":"10.3390\/s18061924"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rai, M., Reshetilov, A., Plekhanova, Y., and Ingle, A.P. (2021). Electrochemical DNA sensors based on nanostructured polymeric materials for determination of antitumor drugs. Macro, Micro and Nano-Biosensors. Potential Applications and Possible Limitations, Springer Nature.","DOI":"10.1007\/978-3-030-55490-3"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4102","DOI":"10.2174\/0929867324666171121103156","article-title":"Electrochemical nucleic acid based biosensing of drugs of abuse and pharmaceuticals","volume":"25","author":"Campuzano","year":"2018","journal-title":"Curr. Med. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.bios.2018.02.034","article-title":"Electrochemical sensors and biosensors for the analysis of antineoplastic drugs","volume":"108","author":"Lima","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1002\/elan.201900286","article-title":"Nanomaterial-based sensors for the study of DNA interaction with drugs","volume":"31","year":"2019","journal-title":"Electroanalysis"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kuzin, Y., Kappo, D., Porfireva, A., Shurpik, D., Stoikov, I., Evtugyn, G., and Hianik, T. (2018). Electrochemical DNA sensor based on carbon black-poly(neutral red) composite for detection of oxidative DNA damage. Sensors, 18.","DOI":"10.3390\/s18103489"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"115718","DOI":"10.1016\/j.trac.2019.115718","article-title":"Recent advances in electrochemical biosensors for antioxidant analysis in foodstuff","volume":"122","author":"Ye","year":"2020","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ab.2016.10.028","article-title":"CUPRAC colorimetric and electroanalytical methods determining antioxidant activity based on prevention of oxidative DNA damage","volume":"518","author":"Uzunboy","year":"2017","journal-title":"Anal. Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1002\/elan.201900722","article-title":"Electrochemical determination of the effect of caffeic acid onto the interaction between idarubicin and DNA by single-use disposable electrodes","volume":"32","author":"Muti","year":"2020","journal-title":"Electroanalysis"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Phopin, K., and Tantimongcolwat, T. (2020). Pesticide aptasensors-sate of the art and perspectives. Sensors, 20.","DOI":"10.3390\/s20236809"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1002\/elan.202060388","article-title":"Electrochemical aptasensors for biological and chemical analyte detection","volume":"33","author":"Topkaya","year":"2021","journal-title":"Electroanalysis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s00216-019-02226-x","article-title":"Electrochemical biosensors based on nucleic acid aptamers","volume":"412","author":"Villalonga","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s00604-019-4034-0","article-title":"A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials","volume":"187","author":"Goud","year":"2020","journal-title":"Microchim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, Z., Mohamed, M.A., Mohan, A.M.V., Zhu, Z., Sharma, V., Mishra, G.K., and Mishra, R.K. (2019). Application of electrochemical aptasensors toward clinical diagnostics, food, and environmental monitoring: Review. Sensors, 19.","DOI":"10.3390\/s19245435"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2018.10.058","article-title":"Electrochemical aptamer-based sensors for food and water analysis: A review","volume":"1051","author":"Li","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.bios.2017.11.028","article-title":"Nucleic acid aptamer-based methods for diagnosis of infections","volume":"102","author":"Park","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1002\/celc.201800848","article-title":"Electrochemical impedance spectroscopy based biosensors: Mechanistic principles, analytical examples and challenges towards commercialization for assays of protein cancer biomarkers","volume":"6","author":"Bertok","year":"2019","journal-title":"ChemElectroChem"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Poghossian, A., and Sch\u00f6ning, M.J. (2020). Capacitive field-effect EIS chemical sensors and biosensors: A status report. Sensors, 20.","DOI":"10.3390\/s20195639"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.1016\/j.biomaterials.2008.12.065","article-title":"Conducting polymers for electrochemical DNA sensing","volume":"30","author":"Peng","year":"2009","journal-title":"Biomaterials"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.trac.2015.11.025","article-title":"Electrochemical DNA sensors and aptasensors based on electropolymerized materials and polyelectrolyte complexes","volume":"79","author":"Evtugyn","year":"2016","journal-title":"TrAC-Trends. Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mohamad, F.S., Zaid, M.H.M., Abdullah, J., Zawawi, R.M., Lim, H.N., Sulaiman, Y., and Rahman, N.A. (2017). Synthesis and characterization of polyaniline\/graphene composite nanofiber and its application as an electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis. Sensors, 17.","DOI":"10.3390\/s17122789"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1007\/s00604-017-2295-z","article-title":"A voltammetric study on the interaction between isoproterenol and cardiomyocyte DNA by using a glassy carbon electrode modified with carbon nanotubes, polyaniline and gold nanoparticles","volume":"184","author":"Wang","year":"2017","journal-title":"Microchim. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"087508","DOI":"10.1149\/1945-7111\/ab8fdb","article-title":"Silver nanoparticles decorated polyaniline nanowires-based electrochemical DNA sensor: Two-step electrochemical synthesis","volume":"167","author":"Tran","year":"2020","journal-title":"J. Electrochem. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s00604-019-3588-1","article-title":"Electrochemical sensors and biosensors based on the use of polyaniline and its nanocomposites: A review on recent advances","volume":"186","author":"Shoaie","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kulikova, T., Porfireva, A., Evtugyn, G., and Hianik, T. (2019). Electrochemical DNA sensors with layered polyaniline-DNA coating for detection of specific DNA interactions. Sensors, 19.","DOI":"10.3390\/s19030469"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.snb.2018.12.164","article-title":"A new epirubicin biosensor based on amplifying DNA interactions with polypyrrole and nitrogen-doped reduced graphene: Experimental and docking theoretical investigations","volume":"284","author":"Khodadadi","year":"2019","journal-title":"Sens. Actuators B"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.trac.2017.10.009","article-title":"Polypyrrole based next generation electrochemical sensors and biosensors: A review","volume":"97","author":"Jain","year":"2017","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.bios.2011.07.051","article-title":"Development of an electrochemical polypyrrole-based DNA sensor and subsequent studies on the effects of probe and target length on performance","volume":"28","author":"Booth","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"B153","DOI":"10.1149\/2.0601605jes","article-title":"Label-free DNA hybridization detection by poly(thionine)-gold nanocomposite on indium tin oxide electrode","volume":"163","author":"Rahman","year":"2016","journal-title":"J. Electrochem. Soc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1002\/elan.200900453","article-title":"Lable-free electrochemical DNA sensor based on gold nanoparticles\/poly(neutral red) modified electrode","volume":"22","author":"Zhang","year":"2010","journal-title":"Electroanalysis"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/elan.201900653","article-title":"Impedimetric DNA sensor based on poly(proflavine) for determination of anthracycline drugs","volume":"32","author":"Porfireva","year":"2020","journal-title":"Electroanalysis"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Porfireva, A., Vorobev, V., Babkina, S., and Evtugyn, G. (2019). Electrochemical sensor based on poly(Azure B)-DNA composite for doxorubicin determination. Sensors, 19.","DOI":"10.3390\/s19092085"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s00604-004-0283-6","article-title":"Electropolymerization of Azure B on a screen-printed carbon electrode and its application to the determination of NADH in a flow injection analysis system","volume":"148","author":"Sha","year":"2004","journal-title":"Microchim. Acta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.apsusc.2018.06.262","article-title":"Formation of poly(Azure A)-C60 photoactive layer as a novel approach in the heterogeneous photogeneration of singlet oxygen","volume":"457","author":"Piwowara","year":"2018","journal-title":"Appl. Surf. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1002\/elan.201800331","article-title":"Voltammetric sensor with replaceable polyaniline-DNA layer for doxorubicin determination","volume":"30","author":"Kulikova","year":"2018","journal-title":"Electroanalysis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/BF01337937","article-title":"Verwendung von Schwingquarzen zur W\u00e4gung d\u00fcnner Schichten und zur Mikrow\u00e4gung","volume":"155","author":"Sauerbrey","year":"1959","journal-title":"Z. Phys."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1021\/ac00285a062","article-title":"Frequency of a quartz microbalance in contact with liquid","volume":"57","author":"Kanazawa","year":"1985","journal-title":"Anal. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0891-5849(87)80003-5","article-title":"Copper-catalyzed DNA damage by ascorbate and hydrogen peroxide: Kinetics and yield","volume":"3","author":"Stoewe","year":"1987","journal-title":"Free Radic. Biol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1002\/elan.201600297","article-title":"Voltammetric detection of oxidative DNA damage based on interactions between polymeric dyes and DNA","volume":"28","author":"Kuzin","year":"2016","journal-title":"Electroanalysis"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0300-9440(02)00222-9","article-title":"EIS studies of coated metals in accelerated exposure","volume":"46","author":"Bierwagen","year":"2003","journal-title":"Progr. Org. Coat."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.aca.2017.12.020","article-title":"A detailed electrochemical impedance spectroscopy study of a bismuth-film glassy carbon electrode for trace metal analysis","volume":"1004","author":"Petovar","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0022-0728(98)00471-9","article-title":"Evaluation of model and dispersion parameters and their effects on the formation of constant-phase elements in equivalent circuits","volume":"464","year":"1999","journal-title":"J. Electroanal. Chem."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/21\/9\/2949\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:51:36Z","timestamp":1760161896000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/21\/9\/2949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,22]]},"references-count":51,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["s21092949"],"URL":"https:\/\/doi.org\/10.3390\/s21092949","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,22]]}}}