{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:28:29Z","timestamp":1793262509483,"version":"4.5.0"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31401091"],"award-info":[{"award-number":["31401091"]}]},{"name":"National Natural Science Foundation of China","award":["ygzrc2024103"],"award-info":[{"award-number":["ygzrc2024103"]}]},{"name":"Talent Project of Chongqing Medical and Pharmaceutical College","award":["31401091"],"award-info":[{"award-number":["31401091"]}]},{"name":"Talent Project of Chongqing Medical and Pharmaceutical College","award":["ygzrc2024103"],"award-info":[{"award-number":["ygzrc2024103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Deltamethrin (Del), a widely utilized pyrethroid pesticide, exhibits significant risks to human health due to its persistent environmental residues. This study aims to develop an efficient sensing detector for rapid Del detection through aptamer-based recognition. A modified Capture-SELEX strategy successfully identified Del-1, a high-affinity DNA aptamer demonstrating specific binding to Del with a dissociation constant (Kd) of 82.90 \u00b1 6.272 nM. Molecular docking analysis revealed strong intermolecular interactions between Del-1 and Del, exhibiting a favorable binding energy of \u22127.35 kcal\u00b7mol\u22121. Leveraging these findings, we constructed a colorimetric detector using gold nanoparticles (AuNPs) and poly dimethyl diallyl ammonium chloride (PDDA)-mediated aggregation modulation. The sensing detector employed dual detection parameters: (1) a characteristic color transition from red to blue and (2) a quantitative \u2206A650\/A520 ratio measurement. This optimized system achieved a detection limit of 54.57 ng\u00b7mL\u22121 with exceptional specificity against other competitive pesticides. Practical validation using spiked fruit samples (apples and pears) yielded satisfactory recoveries of 74\u2013118%, demonstrating the sensor\u2019s reliability in real-sample analysis. The developed methodology presents a promising approach for the on-site monitoring of pyrethroid contaminants in agricultural products.<\/jats:p>","DOI":"10.3390\/s25072060","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T06:58:43Z","timestamp":1742972323000},"page":"2060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Screening of Aptamers and the Development of a Colorimetric Detection Method for the Pesticide Deltamethrin"],"prefix":"10.3390","volume":"25","author":[{"given":"Caixia","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science\/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science\/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2893-3200","authenticated-orcid":false,"given":"Jiafu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Minister of Education), Department of Life Science\/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing 400000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.etap.2018.05.013","article-title":"Assessment of the inhibitory activity of the pyrethroid pesticide deltamethrin against human placental glutathione transferase P1-1: A combined kinetic and docking study","volume":"61","author":"Markus","year":"2018","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1080\/19440049.2017.1420919","article-title":"A review of methodology for the analysis of pyrethrin and pyrethroid residues in food of animal origin","volume":"35","author":"Tuck","year":"2018","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.envres.2018.12.045","article-title":"Deltamethrin toxicity: A review ofoxidative stress and metabolism","volume":"170","author":"Lu","year":"2019","journal-title":"Environ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.envpol.2018.10.030","article-title":"Transcriptional and physiological effects of the pyrethroid deltamethrin and the organophosphate dimethoate in the brain of honey bees (Apis mellifera)","volume":"244","author":"Christen","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s10653-024-02043-x","article-title":"Overview of deltamethrin residues and toxic effects in the global environment","volume":"46","author":"Shi","year":"2024","journal-title":"Environ. Geochem. Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113460","DOI":"10.1016\/j.ecoenv.2022.113460","article-title":"Influence of perinatal deltamethrin exposure at distinct developmental stages on motor activity, learning and memory","volume":"236","author":"Xi","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_7","unstructured":"(2022). List of Carcinogens of the International Research Agency of the World Health Organization Group 3 Carcinogens, Shaoxing Market Supervision and Administration Bureau."},{"key":"ref_8","unstructured":"(2021). Maximum Residue Limits for Pesticides in Food (Standard No. GB 2763\u20132021)."},{"key":"ref_9","first-page":"33","article-title":"Methods for simultaneous determination of methylchlor, hexachlorobenzene and deltamethrin in drinking water by GC-MS\/MS","volume":"4","author":"Yan","year":"2024","journal-title":"J. China Urban Water Assoc."},{"key":"ref_10","first-page":"19","article-title":"Validation of methods for determining bifenthrin, cyhalopitrin and deltamethrin in fruits and vegetables by gas chromatography","volume":"34","author":"Yan","year":"2023","journal-title":"South. Hortic."},{"key":"ref_11","first-page":"1900","article-title":"Study and application of broad spectrum immunochromatographic strips for pyrethroids","volume":"44","author":"Xu","year":"2016","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ces.2015.10.029","article-title":"Cost evaluation of antibody production processes in different operation modes","volume":"141","author":"Klutz","year":"2016","journal-title":"Chem. Eng. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.biochi.2020.08.019","article-title":"Antibody stability: A key to performance\u2014Analysis, influences and improvement","volume":"177","author":"Ma","year":"2020","journal-title":"Biochimie"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/355850a0","article-title":"Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures","volume":"355","author":"Ellington","year":"1992","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"113574","DOI":"10.1016\/j.ab.2020.113574","article-title":"Application of aptamers as molecular recognition elements in lateral flow assays","volume":"593","author":"Reid","year":"2022","journal-title":"Anal. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Lai, B.S., and Juhas, M. (2019). Recent Advances in Aptamer Discovery and Applications. Molecules, 24.","DOI":"10.3390\/molecules24050941"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.bios.2015.04.041","article-title":"Replacing antibodies with aptamers in lateral flow immunoassay","volume":"71","author":"Chen","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_19","first-page":"415697","article-title":"Capture-SELEX: Selection of DNA aptamers for aminoglycoside antibiotics","volume":"1","author":"Stoltenburg","year":"2012","journal-title":"J. Anal. Methods. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"338837","DOI":"10.1016\/j.aca.2021.338837","article-title":"Selection and identification of a DNA aptamer for ultrasensitive and selective detection of \u03bb-cyhalothrin residue in food","volume":"1179","author":"Yang","year":"2021","journal-title":"Anal. Chim. Acta."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"124712","DOI":"10.1016\/j.talanta.2023.124712","article-title":"Screening and identification of a DNA aptamer to construct the label-free fluorescent aptasensor for ultrasensitive and selective detection of clothianidin residue in agricultural products","volume":"262","author":"Yang","year":"2023","journal-title":"Talanta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1039\/C3AN02117C","article-title":"Selection of a DNA aptamer for detection based on cationic polymer mediated aggregation of gold nanoparticles","volume":"139","author":"Wu","year":"2014","journal-title":"Analyst"},{"key":"ref_23","first-page":"567","article-title":"Screening of paraquat nucleic acid aptamer and its colorimetric detection study","volume":"47","author":"Ran","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"340289","DOI":"10.1016\/j.aca.2022.340289","article-title":"Screening and identification of an aptamer as novel recognition molecule in the test strip and its application for visual detection of ethyl carbamate in liquor","volume":"1226","author":"Xia","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5054","DOI":"10.1039\/D2AN01410F","article-title":"A colorimetric aptasensor based on a hemin\/EpCAM aptamer DNAzyme for sensitive exosome detection","volume":"147","author":"Kuang","year":"2022","journal-title":"Analyst"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4105","DOI":"10.1039\/D2AN00668E","article-title":"An aptamer and flower-shaped AuPtRh nanoenzyme-based colorimetric biosensor for the detection of profenofos","volume":"147","author":"Tan","year":"2022","journal-title":"Analyst"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8222","DOI":"10.1021\/acsomega.7b01336","article-title":"Effects of Cationic Proteins on Gold Nanoparticle\/Aptamer Assays","volume":"2","author":"Pires","year":"2017","journal-title":"ACS Omega"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"117809","DOI":"10.1016\/j.saa.2019.117809","article-title":"Colorimetric determination of carbendazim based on the specific recognition of aptamer and the poly-diallyldimethylammonium chloride aggregation of gold nanoparticles","volume":"228","author":"Wang","year":"2020","journal-title":"Spectrochim. Acta A"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1021\/acs.jafc.6b04951","article-title":"Selection and Application of ssDNA Aptamers against Clenbuterol Hydrochloride Based on ssDNA Library Immobilized SELEX","volume":"65","author":"Duan","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e42731","DOI":"10.1371\/journal.pone.0042731","article-title":"Selection of DNA aptamers against glioblastoma cells with high affinity and specificity","volume":"7","author":"Kang","year":"2012","journal-title":"PLoS ONE"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s10529-012-0850-6","article-title":"Selection of DNA aptamers that bind to four organophosphorus pesticides","volume":"34","author":"Wang","year":"2012","journal-title":"Biotechnol. Lett."},{"key":"ref_32","first-page":"309","article-title":"A study on the immunomodulatory mechanism of Hibiscus sabdariffa flavonoids based on network pharmacology and molecular docking probes","volume":"60","author":"Yang","year":"2024","journal-title":"Chin. J. Anim. Husb."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1016\/j.talanta.2010.05.035","article-title":"Simultaneous determination of cypermethrin and permethrin in pear juice by ultrasound-assisted dispersive liquid-liquid microextraction combined with gas chromatography","volume":"82","author":"Du","year":"2010","journal-title":"Talanta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1093\/jat\/21.5.397","article-title":"HPLC determination of flumethrin, deltamethrin, cypermethrin, and cyhalothrin residues in the milk and blood of lactating dairy cows","volume":"21","author":"Bissacot","year":"1997","journal-title":"J. Anal. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1021\/jf0207629","article-title":"Enzyme-linked immunosorbent assay for the pyrethroid deltamethrin","volume":"50","author":"Lee","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"135786","DOI":"10.1016\/j.snb.2024.135786","article-title":"Internal standard-based self-assembly Ag@4-MBN@Ag nanoarray SERS ratio sensor for sensitive detection of deltamethrin in foods","volume":"412","author":"Zhang","year":"2024","journal-title":"Sens. Actuators B Chemical."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"141121","DOI":"10.1016\/j.foodchem.2024.141121","article-title":"Selective and sensitive molecularly imprinted polymer-based electrochemical sensor for detection of deltamethrin","volume":"463","author":"Mutlu","year":"2025","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhu, J.Y., Yin, L.F., Zhang, W.Y., Chen, M.L., Feng, D.S., Zhao, Y., and Zhu, Y.G. (2022). Colorimetric Measurement of Deltamethrin Pesticide Using a Paper Sensor Based on Aggregation of Gold Nanoparticles. Coatings, 12.","DOI":"10.3390\/coatings12010038"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/25\/7\/2060\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:00:21Z","timestamp":1760029221000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/25\/7\/2060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["s25072060"],"URL":"https:\/\/doi.org\/10.3390\/s25072060","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]}}}