{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:21:45Z","timestamp":1790446905338,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union\u2019s Horizon 2020 Research and Innovation Program","doi-asserted-by":"publisher","award":["101007299"],"award-info":[{"award-number":["101007299"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union\u2019s Horizon 2020 Research and Innovation Program","doi-asserted-by":"publisher","award":["1\/0445\/23"],"award-info":[{"award-number":["1\/0445\/23"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Agency VEGA","award":["101007299"],"award-info":[{"award-number":["101007299"]}]},{"name":"Science Agency VEGA","award":["1\/0445\/23"],"award-info":[{"award-number":["1\/0445\/23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>We developed a sensor consisting of V2O5 nanorods and a reduced graphene oxide (rGO) nanocomposite (V2O5\/rGO) with immobilized DNA aptamers (Apt-NH@V2O5\/rGO) for the sensitive electrochemical detection of Hg (II). The V2O5 nanorods anchored on rGO nanosheets were synthesized using a hydrothermal method. The nanocomposite was analyzed by various powerful physical methods that include X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), field emission scanning electron microscopy (FE-SEM), Raman spectroscopy, the Brunauer\u2013Emmett\u2013Teller (BET) method, and Fourier transform infrared spectroscopy (FTIR). The FE-SEM of V2O5 disclosed the nanorod-like structure and uniform anchoring of V2O5 on the rGO nanosheet. Moreover, the BET results showed that the V2O5\/rGO nanocomposite possesses excellent porosity. Furthermore, a glassy carbon electrode (GCE) was modified with Apt-NH@V2O5\/rGO and used for the electrochemical detection of Hg(II) by differential pulse voltammetry (DPV). The aptasensor exhibited excellent sensitivity and selectivity toward Hg(II) detection, with a limit of detection (LOD) of 5.57 nM, which is below the maximum permissible limit established by WHO for rivers (30 nM). The sensor also exhibited significant stability and good repeatability.<\/jats:p>","DOI":"10.3390\/s25072334","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:03:07Z","timestamp":1744174987000},"page":"2334","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Electrochemical Sensor Based on DNA Aptamers Immobilized on V2O5\/rGO Nanocomposite for the Sensitive Detection of Hg(II)"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8345-3870","authenticated-orcid":false,"given":"Mahesh A.","family":"Takte","sequence":"first","affiliation":[{"name":"RUSA Center for Advanced Sensor Technology, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Chhatrapati Sambhajinagar 431004, MS, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2064-528X","authenticated-orcid":false,"given":"Shubham S.","family":"Patil","sequence":"additional","affiliation":[{"name":"RUSA Center for Advanced Sensor Technology, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Chhatrapati Sambhajinagar 431004, MS, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0692-4656","authenticated-orcid":false,"given":"Akash V.","family":"Fulari","sequence":"additional","affiliation":[{"name":"Symbiosis Centre for Nanoscience and Nanotechnology, Symbiosis International (Deemed University), Pune 412115, MS, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5906-0548","authenticated-orcid":false,"given":"Tibor","family":"Hianik","sequence":"additional","affiliation":[{"name":"Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 84248 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahendra D.","family":"Shirsat","sequence":"additional","affiliation":[{"name":"RUSA Center for Advanced Sensor Technology, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Chhatrapati Sambhajinagar 431004, MS, India"},{"name":"Department of Electronics, Dr. Babasaheb Ambedkar Marathwada University, Chhatrapati Sambhajinagar 431004, MS, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125015","DOI":"10.1016\/j.chemosphere.2019.125015","article-title":"A case study on pollution and a human health risk assessment of heavy metals in agricultural soils around Sinop province, Turkey","volume":"241","author":"Baltas","year":"2020","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101653","DOI":"10.1016\/j.jksus.2021.101653","article-title":"Health and environmental effects of heavy metals","volume":"34","author":"Zaynab","year":"2022","journal-title":"J. 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