{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:47:50Z","timestamp":1788806870935,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T00:00:00Z","timestamp":1749081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Marche Region, Rural Development Program 2014\/22-M16.1.A.2 Funding Operational Groups","award":["41377"],"award-info":[{"award-number":["41377"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>The wine industry faces increasing challenges related to authenticity, safety, and sustainability due to recurrent fraud, shifting consumer preferences, and environmental concerns. In this study, as part of the B.I.O.C.E.R.T.O project, we integrated blockchain technology with ultrasonic spectroscopy and soil quality data by using the arthropod-based Soil Biological Quality Index (QBS-ar) to enhance traceability, ensure wine quality, and certify sustainable vineyard practices. Four representative wines from the Marche region (Sangiovese, Maceratino, and two Verdicchio PDO varieties) were analyzed across two vintages (2021 and 2022). Ultrasound spectroscopy demonstrated high sensitivity in distinguishing wines based on ethanol and sugar content, comparably to conventional viscosity-based methods. The QBS-ar index was applied to investigate the soil biodiversity status according to the agricultural management practices applied in each vineyard, reinforcing consumer confidence in environmentally responsible viticulture. By recording these data on a public blockchain, we developed a secure, transparent, and immutable certification system to verify the geographical origin of wines along with their unique characteristics. This is the first study to integrate advanced analytical techniques with blockchain technology for wine traceability, simultaneously addressing counterfeiting, consumer demand for transparency, and biodiversity preservation. Our findings support the applicability of this model to other agri-food sectors, with potential for expansion through additional analytical techniques, such as isotopic analysis and further agroecosystem sustainability indicators.<\/jats:p>","DOI":"10.3390\/s25113567","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T09:43:18Z","timestamp":1749116598000},"page":"3567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A.A.A. Good Wines WANTED: Blockchain, Non-Destructive Ultrasonic Techniques and Soil Health Assessment for Wine Traceability"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-4150","authenticated-orcid":false,"given":"Diego Romano","family":"Perinelli","sequence":"first","affiliation":[{"name":"School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2775-9953","authenticated-orcid":false,"given":"Martina","family":"Coletta","sequence":"additional","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8125-8542","authenticated-orcid":false,"given":"Beatrice","family":"Sabbatini","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3608-140X","authenticated-orcid":false,"given":"Aldo","family":"D\u2019Alessandro","sequence":"additional","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Fabiani","sequence":"additional","affiliation":[{"name":"EGG CHAIN S.r.l., Via Le Piane 23B, 62036 Pieve Torina, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Passacantando","sequence":"additional","affiliation":[{"name":"Azienda Agricola Passacantando Andrea, C. da Ributino 7, 62029 Tolentino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8528-4166","authenticated-orcid":false,"given":"Giulia","family":"Bonacucina","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-1503","authenticated-orcid":false,"given":"Antonietta","family":"La Terza","sequence":"additional","affiliation":[{"name":"School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"key":"ref_1","unstructured":"(2025, February 03). ISMEA-Relazione Vendemmiale. Available online: https:\/\/www.ismeamercati.it\/flex\/cm\/pages\/ServeBLOB.php\/L\/IT\/IDPagina\/13248."},{"key":"ref_2","unstructured":"Coldiretti (2025, February 04). Frodi a Tavola Fanno Paura a due Italiani su tre. Available online: https:\/\/www.coldiretti.it\/economia\/frodi-paura-tavola-due-italiani-3."},{"key":"ref_3","unstructured":"WineNews (2025, February 04). Truffa del Verdicchio: 150000 litri di vino Bianco Comune Spacciati Come DOC. 4 June 2018. Available online: https:\/\/winenews.it\/it\/truffa-del-verdicchio-150-000-litri-di-vino-bianco-comune-spacciati-come-doc_365164\/."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Pop\u00eerd\u0103, A., Luchian, C.E., Cotea, V.V., Colibaba, L.C., Scutara\u0219u, E.C., and Toader, A.M. (2021). A Review of Representative Methods Used in Wine Authentication. Agriculture, 11.","DOI":"10.3390\/agriculture11030225"},{"key":"ref_5","unstructured":"Joint Research Centre-European Commission (2025, February 07). Wine Fraud\u2013How Genuine Is Your Wine? Adulteration of Wine Is a Worldwide Issue Affecting Consumers. The JRC Just Released a Certified Reference Material (CRM) to Verify the Authenticity of Wines (27 July 2021). Available online: https:\/\/joint-research-centre.ec.europa.eu\/jrc-news-and-updates\/wine-fraud-how-genuine-your-wine-2021-07-27_en."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Vitaskos, V., Demestichas, K., Karetsos, S., and Costopoulou, C. (2024). Blockchain and Internet of Things Technologies for Food Traceability in Olive Oil Supply Chains. Sensors, 24.","DOI":"10.20944\/preprints202410.0342.v1"},{"key":"ref_7","first-page":"50","article-title":"A Framework for Food Traceability: Case Study\u2013Italian Extra-Virgin Olive Oil Supply Chain","volume":"11","author":"Guido","year":"2020","journal-title":"Int. J. Ind. Eng. Manag."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Violino, S., Pallottino, F., Sperandio, G., Figorilli, S., Ortenzi, L., Tocci, F., Vasta, S., Imperi, G., and Costa, C. (2020). A Full Technological Traceability System for Extra Virgin Olive Oil. Foods, 9.","DOI":"10.3390\/foods9050624"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100117","DOI":"10.1016\/j.clscn.2023.100117","article-title":"Blockchain and consumer behaviour: Results of a Technology Acceptance Model in the ancient wheat sector","volume":"8","author":"Bandinelli","year":"2023","journal-title":"Clean. Logist. Supply Chain"},{"key":"ref_10","first-page":"130","article-title":"Blockchain Applications in the Food Industry: A Pilot Project Implementation in the Ancient Grains Industry","volume":"702","author":"Bindi","year":"2024","journal-title":"IFIP Advances in Information and Communication Technology"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Corbacho, J.\u00c1., Morcuende, D., Rufo, M., Paniagua, J.M., Ontalba, M.\u00c1., and Jim\u00e9nez, A. (2024). Use of Non-Destructive Ultrasonic Techniques as Characterization Tools for Different Varieties of Wine. Sensors, 24.","DOI":"10.3390\/s24134294"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.ijpharm.2016.03.009","article-title":"Acoustic spectroscopy: A powerful analytical method for the pharmaceutical field?","volume":"503","author":"Bonacucina","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"106384","DOI":"10.1016\/j.ultsonch.2023.106384","article-title":"The state-of-the-art research of the application of ultrasound to winemaking: A critical review","volume":"95","author":"Zhang","year":"2023","journal-title":"Ultrason. Sonochem."},{"key":"ref_14","unstructured":"Garc\u00eda Mart\u00edn, J.F. (2016). Applications of Ultrasound in the Beverage Industry, Nova Publishers."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10408399709527766","article-title":"Ultrasonic characterization of foods and drinks: Principles, methods, and applications","volume":"37","author":"McClements","year":"1997","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Breazeale, M.A., and McPherson, M. (2007). Physical Acoustics. Handbook of Acoustics, Springer.","DOI":"10.1007\/978-0-387-30425-0_6"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Delmas, H., Barthe, L., and Cleary, R. (2023). Ultrasonic mixing, homogenization, and emulsification in food processing and other applications. Power Ultrasonics: Applications of High-Intensity Ultrasound, Woodhead Publishing. [2nd ed.].","DOI":"10.1016\/B978-0-12-820254-8.00020-8"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ultras.2015.09.001","article-title":"Characterization of mechanical properties of materials using ultrasound broadband spectroscopy","volume":"64","author":"Agrawal","year":"2016","journal-title":"Ultrasonics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1111\/srt.13033","article-title":"Characterization of skin aging through high-frequency ultrasound imaging as a technique for evaluating the effectiveness of anti-aging products and procedures: A review","volume":"27","author":"Vergilio","year":"2021","journal-title":"Ski. Res. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dissanayake, D. (2010). Ultrasonics: A Technique of Material Characterization. Acoustic Waves, InTech.","DOI":"10.5772\/277"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1016\/j.ultras.2014.04.004","article-title":"Ultrasonic monitoring of malolactic fermentation in red wines","volume":"54","author":"Amer","year":"2014","journal-title":"Ultrasonics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.ultras.2008.06.003","article-title":"An ultrasound technique for monitoring the alcoholic wine fermentation","volume":"49","author":"Lamberti","year":"2009","journal-title":"Ultrasonics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.wep.2019.09.002","article-title":"Consumers\u2019 preferences for biodiversity in vineyards: A choice experiment on wine","volume":"8","author":"Mazzocchi","year":"2019","journal-title":"Wine Econ. Policy"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ghiglieno, I., Simonetto, A., Sperandio, G., Ventura, M., Gatti, F., Donna, P., Tonni, M., Valenti, L., and Gilioli, G. (2021). Impact of Environmental Conditions and Management on Soil Arthropod Communities in Vineyard Ecosystems. Sustainability, 13.","DOI":"10.3390\/su132111999"},{"key":"ref_25","first-page":"105","article-title":"La qualit\u00e0 biologica del suolo.Un metodo basato sui microartropodi","volume":"37","author":"Parisi","year":"2001","journal-title":"ACTA Nat. L\u2019ATENEO Parm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.agee.2004.02.002","article-title":"Microarthropod communities as a tool to assess soil quality and biodiversity: A new approach in Italy","volume":"105","author":"Parisi","year":"2005","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s10661-025-13658-7","article-title":"Managing soil to support soil biodiversity in protected areas agroecosystems: A comparison between arable lands, olive groves, and vineyards in the Conero Park (Italy)","volume":"197","author":"Coletta","year":"2025","journal-title":"Environ. Monit. Assess."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"106666","DOI":"10.1016\/j.cropro.2024.106666","article-title":"Below-ground arthropod diversity in conventional and organic vineyards: A review","volume":"180","author":"Nardi","year":"2024","journal-title":"Crop Prot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1007\/s11368-023-03447-5","article-title":"A magnifying glass on biochar strategy: Long-term effects on the soil biota of a Tuscan vineyard","volume":"23","author":"Maienza","year":"2023","journal-title":"J. Soils Sediments"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ghiglieno, I., Simonetto, A., Donna, P., Tonni, M., Valenti, L., Bedussi, F., and Gilioli, G. (2019). Soil Biological Quality Assessment to Improve Decision Support in the Wine Sector. Agronomy, 9.","DOI":"10.3390\/agronomy9100593"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"106863","DOI":"10.1016\/j.agee.2020.106863","article-title":"Do soil management practices affect the activity density, diversity, and stability of soil arthropods in vineyards?","volume":"294","author":"Nunes","year":"2020","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"89","DOI":"10.36253\/wep-16278","article-title":"Exploring blockchain adoption in the italian wine industry: Insights from a multiple case study","volume":"13","author":"Cricelli","year":"2024","journal-title":"Wine Econ. Policy"},{"key":"ref_33","unstructured":"Agenzia Servizi Settore Agroalimentare delle Marche (A.S.S.A.M.) (2006). Suoli e Paesaggi delle Marche, Errebi Grafiche Ripesi."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"207","DOI":"10.5194\/jsss-7-207-2018","article-title":"High-resolution ultrasonic spectroscopy","volume":"7","author":"Buckin","year":"2018","journal-title":"J. Sens. Sens. Syst."},{"key":"ref_35","unstructured":"Dazzi, C. (2011). Il ruolo della fauna edafica nel mantenimento della funzionalit\u00e0 del suolo. La Percezione del Suolo, Le Penseur."},{"key":"ref_36","unstructured":"R Core Team (2021). A Language and Environment for Statistical Computing, Foundation for Statistical Computing."},{"key":"ref_37","unstructured":"(2025, April 23). Quadrans Blockchain. Available online: https:\/\/quadrans.io\/."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Yaa\u2019ri, R., Schneiderman, E., Ben Aharon, V., Stanevsky, M., and Drori, E. (2024). Development of a Novel Approach for Controlling and Predicting Residual Sugars in Wines. Fermentation, 10.","DOI":"10.20944\/preprints202402.0058.v2"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"36","DOI":"10.32732\/jfet.2022.11.1.36","article-title":"Correlation of Wine\u2019s Main Components\u2019 Concentration with the Density of Model Aqueous Solutions and Wine Samples","volume":"11","author":"Evangelou","year":"2022","journal-title":"J. Food Eng. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.ejsobi.2006.10.002","article-title":"Soil invertebrates and ecosystem services","volume":"42","author":"Lavelle","year":"2006","journal-title":"Eur. J. Soil Biol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"D\u2019Alessandro, A., Coletta, M., Torresi, A., Dell\u2019Ambrogio, G., Renaud, M., Ferrari, B.J.D., and La Terza, A. (2024). Evaluation of the Impact of Plant Protection Products (PPPs) on Non-Target Soil Organisms in the Olive Orchard: Drone (Aerial) Spraying vs. Tractor (Ground) Spraying. Sustainability, 16.","DOI":"10.3390\/su162411302"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Menta, C., Conti, F.D., Fond\u00f3n, C.L., Staffilani, F., and Remelli, S. (2020). Soil Arthropod Responses in Agroecosystem: Implications of Different Management and Cropping Systems. Agronomy, 10.","DOI":"10.3390\/agronomy10070982"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3958\/059.036.0102","article-title":"Effects of Tillage on Soil Microarthropods in Winter Wheat","volume":"36","author":"Dubie","year":"2011","journal-title":"Southwest. Entomol."},{"key":"ref_44","first-page":"1170","article-title":"Microarthropods of the soil: Convergence phenomena and evaluation of soil quality using QBS-ar and QBS-C","volume":"17","author":"Parisi","year":"2008","journal-title":"Fresenius Environ. Bull."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.ecolind.2017.11.030","article-title":"Soil Biological Quality index (QBS-ar): 15 years of application at global scale","volume":"85","author":"Menta","year":"2018","journal-title":"Ecol. Indic."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.foodchem.2014.08.051","article-title":"The 87Sr\/86Sr strontium isotopic systematics applied to Glera vineyards: A tracer for the geographical origin of the Prosecco","volume":"170","author":"Petrini","year":"2015","journal-title":"Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"101039","DOI":"10.1016\/j.rhisph.2025.101039","article-title":"Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils","volume":"34","author":"Aguiar","year":"2025","journal-title":"Rhizosphere"}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/25\/11\/3567\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:47:12Z","timestamp":1760032032000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/25\/11\/3567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,5]]},"references-count":47,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["s25113567"],"URL":"https:\/\/doi.org\/10.3390\/s25113567","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,5]]}}}