{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:17:40Z","timestamp":1791256660714,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["62301494"],"award-info":[{"award-number":["62301494"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["62301583"],"award-info":[{"award-number":["62301583"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100022963","name":"Key research and development program of Zhejiang Province","doi-asserted-by":"crossref","award":["2024C03055"],"award-info":[{"award-number":["2024C03055"]}],"id":[{"id":"10.13039\/100022963","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds of Zhejiang Sci-Tech University","award":["24222125-Y"],"award-info":[{"award-number":["24222125-Y"]}]},{"name":"General Scientific Research Project of Zhejiang Education Department","award":["Y202457132"],"award-info":[{"award-number":["Y202457132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>To meet the demand for rapid and accurate glucose determination in clinical diagnostics, food testing, and related fields, this study developed a high-performance electrochemical glucose biosensor based on multi-walled carbon nanotubes\/Prussian blue\/zeolitic imidazolate framework-8@glucose oxidase\/chitosan (MWCNTs\/PB\/ZIF-8@GOx\/CS). The MWCNTs\/PB conductive network significantly accelerated electron transfer and catalytic activity, while the ZIF-8, with its regular pore structure and high specific surface area, provides an efficient microenvironment for the immobilization and conformational stabilization of glucose oxidase (GOx), thereby improving substrate diffusion and maintaining enzyme activity. The MWCNTs\/PB\/ZIF-8@GOx\/CS sensor demonstrates excellent sensing performance, featuring a wide linear response to glucose concentrations ranging from 4.8 \u03bcM to 2.24 mM, a high sensitivity of 579.57 \u03bcA\/mM\/cm2, and a low detection limit of 0.55 \u03bcM (S\/N = 3). In addition, the sensor performs excellent repeatability (RSD = 1.49%) and retained 86.23% of its initial response after 3 weeks of storage at 4 \u00b0C, highlighting its strong potential for practical application in glucose detection.<\/jats:p>","DOI":"10.3390\/s25227064","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T15:03:15Z","timestamp":1763564595000},"page":"7064","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A ZIF-8-Based High-Performance Glucose Electrochemical Detection Platform Constructed Using a Multi-Layer Interface Optimization Strategy"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2589-2268","authenticated-orcid":false,"given":"Canjie","family":"Hu","sequence":"first","affiliation":[{"name":"School of Information Science and Engineering (School of Cyber Science and Technology), Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6595-6909","authenticated-orcid":false,"given":"Pengjia","family":"Qi","sequence":"additional","affiliation":[{"name":"School of Information Science and Engineering (School of Cyber Science and Technology), Zhejiang Sci-Tech University, Hangzhou 310018, China"},{"name":"Zhejiang Key Laboratory of Research and Translation for Kidney Deficiency-Stasis-Turbidity Disease, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichao","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Naval Architecture and Ocean Engineering, Naval University of Engineering, Wuhan 430033, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology (School of Artificial Intelligence), Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6209-6605","authenticated-orcid":false,"given":"Jijun","family":"Tong","sequence":"additional","affiliation":[{"name":"School of Information Science and Engineering (School of Cyber Science and Technology), Zhejiang Sci-Tech University, Hangzhou 310018, China"},{"name":"Zhejiang Key Laboratory of Research and Translation for Kidney Deficiency-Stasis-Turbidity Disease, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1038\/s41591-021-01418-2","article-title":"100 Years of Insulin: Celebrating the Past, Present and Future of Diabetes Therapy","volume":"27","author":"Sims","year":"2021","journal-title":"Nat. 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