{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:26:06Z","timestamp":1785432366882,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01 DK121351"],"award-info":[{"award-number":["R01 DK121351"]}]},{"award":["R01 DK121351"],"award-info":[{"award-number":["R01 DK121351"]}],"id":[{"id":"https:\/\/ror.org\/044zkrt47","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Serum contains diverse proteins whose concentrations vary with pathological conditions such as cancer, liver disease, neurological disorder, and infections. Conventional methods like serum protein electrophoresis (SPEP) and enzyme-linked immunosorbent assay (ELISA) are gold standards for protein identification; however, they are time-consuming and can miss abnormal serum protein levels. Inspired by chemical nose sensing based on selective sensor\u2013analyte interactions, we synthesized five pyrene-conjugated fluorescent polymers (PFPs) with distinct side-chain head groups to construct a multichannel fluorescence sensor array. These polymers were screened for sensitivity to changes in serum protein levels using linear discriminant analysis (LDA), a machine learning method. This process led to the successful discovery of two PFPs that effectively detect protein level fluctuations. These PFPs provided a sensitive sensor array capable of generating a high-content response pattern (fingerprint) with six fluorescence channels. This sensor array successfully discriminated protein level fluctuations in serum with 98% jackknife classification accuracy and 95% unknown identification accuracy. This polymer sensor array holds strong potential as a diagnostic tool for serum-based samples and can be extended to other applications related to protein identification.<\/jats:p>","DOI":"10.3390\/s26082308","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T08:56:07Z","timestamp":1775724967000},"page":"2308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Sensitive Multichannel Fluorescent Polymer Sensor Array for the Detection of Protein Fluctuations in Serum"],"prefix":"10.3390","volume":"26","author":[{"given":"Junwhee","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colby","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanwal","family":"Nazir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4742-405X","authenticated-orcid":false,"given":"Mingdi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1016-1171","authenticated-orcid":false,"given":"Nicolas","family":"Araujo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5184-5439","authenticated-orcid":false,"given":"Vincent M.","family":"Rotello","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125858","DOI":"10.1155\/2013\/125858","article-title":"Serum Biomarkers Identification by Mass Spectrometry in High-Mortality Tumors","volume":"2013","author":"Tessitore","year":"2013","journal-title":"Int. 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