{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:14:53Z","timestamp":1785420893118,"version":"3.56.0"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS","award":["25350977"],"award-info":[{"award-number":["25350977"]}]},{"name":"JSPS","award":["19H02852"],"award-info":[{"award-number":["19H02852"]}]},{"name":"JSPS","award":["25K08858"],"award-info":[{"award-number":["25K08858"]}]},{"award":["25350977"],"award-info":[{"award-number":["25350977"]}],"id":[{"id":"https:\/\/ror.org\/02m7axw05","id-type":"ROR","asserted-by":"publisher"}]},{"award":["19H02852"],"award-info":[{"award-number":["19H02852"]}],"id":[{"id":"https:\/\/ror.org\/02m7axw05","id-type":"ROR","asserted-by":"publisher"}]},{"award":["25K08858"],"award-info":[{"award-number":["25K08858"]}],"id":[{"id":"https:\/\/ror.org\/02m7axw05","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>Alterations in the ratio of oxidized and reduced nicotinamide adenine dinucleotide (NAD+\/NADH) reflect the intracellular redox state and have been implicated in a broad spectrum of pathological conditions, including neurogenetic disorders, heart failure, and liver diseases. In the present study, we demonstrate the selective detection of probe-derived NAD+ and NADH signals in mouse liver extracts by means of a triple-resonance nuclear magnetic resonance (NMR) spectroscopy technique. We prepared 13C\/15N-enriched nicotinamide riboside (13C\/15N-NR), which undergoes enzymatic conversion to NAD+ and NADH in the liver, and detected these labeled metabolites by 1H\u2013{13C\u201315N} triple-resonance NMR measurements. This study provides a methodological proof-of-concept for the selective detection of NAD-related signals derived from a stable-isotope labeled probe in mouse liver extracts.<\/jats:p>","DOI":"10.3390\/s26092714","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:33:37Z","timestamp":1777376017000},"page":"2714","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Triple-Resonance NMR Strategy for the Selective Detection of NAD+ and NADH Derived from a 13C\/15N-Nicotinamide Riboside Probe in the Liver Extracts of Mice"],"prefix":"10.3390","volume":"26","author":[{"given":"Hiroki","family":"Shimada","sequence":"first","affiliation":[{"name":"Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijyosanjima-cho, Tokushima 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusei","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijyosanjima-cho, Tokushima 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Uto","sequence":"additional","affiliation":[{"name":"Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijyosanjima-cho, Tokushima 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1611-438X","authenticated-orcid":false,"given":"Hisatsugu","family":"Yamada","sequence":"additional","affiliation":[{"name":"Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 2-1 Minamijyosanjima-cho, Tokushima 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.cmet.2015.05.023","article-title":"NAD+ metabolism and the control of energy homeostasis: A balancing act between mitochondria and the nucleus","volume":"22","author":"Menzies","year":"2015","journal-title":"Cell Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1073\/pnas.1718819115","article-title":"NAD+ supplementation normalizes key Alzheimer\u2019s features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency","volume":"115","author":"Hou","year":"2018","journal-title":"Proc. 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