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One example is cerebral ischemic disease, where many biomarkers are released and provide valuable information about the severity and progression of the disease. In this study, a long-period fiber grating (LPFG) biosensor was developed using a standard single-mode optical fiber and monoclonal antibody (IL-6 mAb) as the biological recognition element to detect IL-6, which is a protein associated with the inflammatory process. The assembly of the LPFG biosensor was characterized through optical and electronic microscopy to observe morphological changes at different stages of fabrication and the detection process. Additionally, micro-infrared spectroscopy was employed to identify functional groups in the protein region linked to the presence of IL-6. Experimental data were analyzed using principal component analysis, confirming the biosensor\u2019s ability to detect IL-6 and providing insights into its fabrication process.<\/jats:p>","DOI":"10.3390\/s26092855","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T01:12:09Z","timestamp":1777857129000},"page":"2855","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LPFG Biosensor for IL-6 Detection in Murine Serum Samples Associated with Ischemic Disease"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7461-9715","authenticated-orcid":false,"given":"Brenda","family":"Vertti-Cervantes","sequence":"first","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, Centro de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada, Ex-Hacienda San Juan Molino Carretera Estatal Tecuexcomac, Tepetitla Km 1.5, Tlaxcala C.P. 90700, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3059-1521","authenticated-orcid":false,"given":"Karina","family":"Gonz\u00e1lez-Le\u00f3n","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias F\u00edsico Matem\u00e1ticas, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Avenida San Claudio y 18 Sur, Col. 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