{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T09:27:25Z","timestamp":1784366845599,"version":"3.55.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Pharm."],"abstract":"<jats:p>Conjugation of curcumin and gold with green chemistry is an approach to improve the effectiveness of curcumin as anti-fibrosis. In this work, curcumin and gold were conjugated to deliver curcumin to the liver. Curcumin-gold nanoparticles (cAuNPs) were prepared by varying curcumin pH and concentration. The successful of cAuNPs formation were identified by using UV-visible and FTIR spectrophotometers. The particle size and morphology were analyzed using particle size analyzer and cryo-TEM respectively. In vitro antioxidant assay was performed to determine the curcumin activity after conjugation. Physical and chemical stabilities of cAuNPs were studied for one month at 5 \u00b0C, 25 \u00b0C, and 40 \u00b0C. Furthermore, the cAuNPs activity to modulate early marker of fibrosis was tested on NIH\/3T3 cells. The optimum condition for cAuNPs synthesis was by using 1.5 mM curcumin at pH 9.3. As compared to free curcumin, cAuNPs showed higher antioxidant activity and maintained the nanosize after stored for one month. In line with the antioxidant activity, cAuNPs 0.25\u20131 \u03bcg\/mL reduced the collagen production by NIH\/3T3 cells. More importantly, cAuNPs did not demonstrate any effect on the development of chicken embryo. Taken together, the attachment of gold to curcumin in the form of cAuNPs is promising for curcumin targeting to treat hepatic fibrosis.<\/jats:p>","DOI":"10.3390\/scipharm86040041","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T11:00:25Z","timestamp":1537527625000},"page":"41","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Antifibrotic Activity and In Ovo Toxicity Study of Liver-Targeted Curcumin-Gold Nanoparticle"],"prefix":"10.3390","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-9423","authenticated-orcid":false,"given":"Amirah","family":"Adlia","sequence":"first","affiliation":[{"name":"School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilham","family":"Tomagola","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia"},{"name":"Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar 90231, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophi","family":"Damayanti","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ardyanto","family":"Mulya","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heni","family":"Rachmawati","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1002\/hep.21347","article-title":"Global challenges in liver disease","volume":"44","author":"Williams","year":"2006","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.jhep.2012.12.005","article-title":"The burden of liver disease in Europe: A review of available epidemiological data","volume":"58","author":"Blachier","year":"2013","journal-title":"J. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4254\/wjh.v4.i3.66","article-title":"Liver diseases in developing countries","volume":"4","author":"Cainelli","year":"2012","journal-title":"World J. Hepatol."},{"key":"ref_4","unstructured":"Metrakos, P., and Nilsson, T. (2017). Non-alcoholic fatty liver disease-a chronic disease of the 21(st) century. J. Biomed. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1055\/s-2000-9506","article-title":"Epidemiology of hepatitis C: Geographic differences and temporal trends","volume":"20","author":"Wasley","year":"2000","journal-title":"Semin. Liver Dis."},{"key":"ref_6","first-page":"3462","article-title":"Alcoholic liver disease and hepatitis C: A frequently underestimated combination","volume":"15","author":"Mueller","year":"2009","journal-title":"World J. Gastroenterol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.pharmthera.2017.10.009","article-title":"Direct antiviral agents (DAAs)\u2014A new age in the treatment of hepatitis C virus infection","volume":"183","author":"Spengler","year":"2018","journal-title":"Pharmacol. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1080\/17512433.2017.1313111","article-title":"Why highly effective drugs are not enough: The need for an affordable solution to eliminating HCV","volume":"10","author":"Hayes","year":"2017","journal-title":"Expert Rev. Clin. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.dld.2006.11.004","article-title":"Herbal medicine in the treatment of liver diseases","volume":"39","author":"Stickel","year":"2007","journal-title":"Dig. Liver Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/S1542-3565(04)00455-0","article-title":"Use of herbal supplements for chronic liver disease","volume":"2","author":"Levy","year":"2004","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e40","DOI":"10.1097\/MCG.0b013e3181b766ed","article-title":"Complementary and alternative medicine use in chronic liver disease patients","volume":"44","author":"Ferrucci","year":"2010","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1097\/MCG.0000000000000617","article-title":"Complementary and Alternative Medicine Use in United States Adults with Liver Disease","volume":"51","author":"Henson","year":"2017","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.2174\/1389450116666150531155711","article-title":"Natural Products on Nonalcoholic Fatty Liver Disease","volume":"16","author":"Xu","year":"2015","journal-title":"Curr. Drug Targets"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jddst.2017.03.015","article-title":"Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis","volume":"39","author":"Rachmawati","year":"2017","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.toxlet.2013.06.240","article-title":"Curcumin ameliorates intrahepatic angiogenesis and capillarization of the sinusoids in carbon tetrachloride-induced rat liver fibrosis","volume":"222","author":"Yao","year":"2013","journal-title":"Toxicol. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1111\/jcmm.12286","article-title":"Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells","volume":"18","author":"Zhang","year":"2014","journal-title":"J. Cell. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1039\/C5FO00176E","article-title":"The effect of curcumin on liver fibrosis in the rat model of microsurgical cholestasis","volume":"6","author":"Barta","year":"2015","journal-title":"Food Funct."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"18767","DOI":"10.3390\/molecules191118767","article-title":"Curcumin protects against CCl4-induced liver fibrosis in rats by inhibiting HIF-1alpha through an ERK-dependent pathway","volume":"19","author":"Zhao","year":"2014","journal-title":"Molecules"},{"key":"ref_19","first-page":"115","article-title":"Curcumin coated gold nanoparticles: Synthesis, characterization, cytotoxicity, antioxidant activity and its comparison with citrate coated gold nanoparticles","volume":"4","author":"Shaabani","year":"2017","journal-title":"Nanomed. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.etap.2013.09.002","article-title":"Green nanotechnology\u2014A new hope for medical biology","volume":"36","author":"Nath","year":"2013","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1016\/j.biomaterials.2007.12.037","article-title":"Particle size-dependent organ distribution of gold nanoparticles after intravenous administration","volume":"29","author":"Hagens","year":"2008","journal-title":"Biomaterials"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"673","DOI":"10.2217\/nnm.16.5","article-title":"The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction","volume":"11","author":"Hoshyar","year":"2016","journal-title":"Nanomedicine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.pharep.2014.10.019","article-title":"The influence of the route of administration of gold nanoparticles on their tissue distribution and basic biochemical parameters: In vivo studies","volume":"67","author":"Bednarski","year":"2015","journal-title":"Pharmacol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.jcis.2011.11.024","article-title":"Gold nanoparticles generated and stabilized by water soluble curcumin-polymer conjugate: Blood compatibility evaluation and targeted drug delivery onto cancer cells","volume":"368","author":"Manju","year":"2012","journal-title":"J. Colloid Interface Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1039\/c2nr33776b","article-title":"In situ synthesis and surface functionalization of gold nanoparticles with curcumin and their antioxidant properties: An experimental and density functional theory investigation","volume":"5","author":"Singh","year":"2013","journal-title":"Nanoscale"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1039\/C3RA45345F","article-title":"Curcumin conjugated gold nanoparticle synthesis and its biocompatibility","volume":"4","author":"Sindhu","year":"2014","journal-title":"RSC Adv."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1002\/hep.22400","article-title":"Pathogenic role of natural killer T and natural killer cells in acetaminophen-induced liver injury in mice is dependent on the presence of dimethyl sulfoxide","volume":"48","author":"Masson","year":"2008","journal-title":"Hepatology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jphotochemrev.2009.05.001","article-title":"Photophysics, photochemistry and photobiology of curcumin: Studies from organic solutions, bio-mimetics and living cells","volume":"10","author":"Priyadarsini","year":"2009","journal-title":"J. Photochem. Photobiol. C Photochem. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1166\/nnl.2013.1678","article-title":"Green Synthesis of Curcumin Capped Gold Nanoparticles and Evaluation of Their Cytotoxicty","volume":"5","author":"Sreelakshmi","year":"2018","journal-title":"Nanosci. Nanotechnol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.1016\/j.msec.2012.07.022","article-title":"Conjugation of curcumin with PVP capped gold nanoparticles for improving bioavailability","volume":"32","author":"Gangwar","year":"2012","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_31","first-page":"2231","article-title":"A comparative study on the antioxidant and immunomodulatory properties of curcumin conjugated gold nanospheres and free curcumin","volume":"7","author":"Singh","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1080\/00071668.2017.1413234","article-title":"The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system","volume":"59","author":"Farzaneh","year":"2018","journal-title":"Br. Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1038\/1811199a0","article-title":"Antioxidant Determinations by the Use of Stable Free Radical","volume":"181","author":"Blois","year":"1958","journal-title":"Nature"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0378-5173(02)00323-X","article-title":"Studies of curcumin and curcuminoids. XXVII. Cyclodextrin complexation: Solubility, chemical and photochemical stability","volume":"244","author":"Tonnesen","year":"2002","journal-title":"Int. J. Pharm."},{"key":"ref_35","first-page":"820","article-title":"Solubility, chemical and photochemical stability of curcumin in surfactant solutions. Studies of curcumin and curcuminoids, XXVIII","volume":"57","author":"Tonnesen","year":"2002","journal-title":"Pharmazie"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1016\/S0731-7085(96)02024-9","article-title":"Stability of curcumin in buffer solutions and characterization of its degradation products","volume":"15","author":"Wang","year":"1997","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1021\/cr068445e","article-title":"Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applictions","volume":"108","author":"Laurent","year":"2008","journal-title":"Chem. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5672","DOI":"10.1021\/la800780w","article-title":"Encapsulation of curcumin in cationic micelles suppresses alkaline hydrolysis","volume":"24","author":"Leung","year":"2008","journal-title":"Langmuir"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e996","DOI":"10.1038\/cddis.2013.499","article-title":"Molecular mechanisms of hepatic apoptosis","volume":"5","author":"Wang","year":"2014","journal-title":"Cell Death Dis."}],"container-title":["Scientia Pharmaceutica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-0532\/86\/4\/41\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:21:58Z","timestamp":1760196118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-0532\/86\/4\/41"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,21]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["scipharm86040041"],"URL":"https:\/\/doi.org\/10.3390\/scipharm86040041","relation":{},"ISSN":["2218-0532"],"issn-type":[{"value":"2218-0532","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,21]]}}}