{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:11:08Z","timestamp":1781788268436,"version":"3.54.5"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["16-14-00028"],"award-info":[{"award-number":["16-14-00028"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sustainability"],"abstract":"<jats:p>A microbial enhanced oil recovery (MEOR) technique was tested at low-temperature heavy oil reservoirs (Russia). The bioaugmentation approach used is based on the introduction of hydrocarbon-oxidizing bacteria into the oilfield in combination with an injection of oxygen as a H2O2 solution in order to initiate the first stage of hydrocarbon oxidation and of (NH4)2HPO4 as a source of biogenic elements. Before the pilot trials, the microorganisms of petroleum reservoirs were investigated by high-throughput sequencing, as well as by culture-base and radioisotope techniques. Molecular studies revealed the differences in microbial composition of the carbonate and terrigenous oil reservoirs and the communities of injection and formation water. Aerobic bacteria Rhodococcus erythropolis HO-KS22 and Gordonia amicalis 6-1 isolated from oilfields oxidized oil and produced biosurfactants. Fermentative enrichment and pure cultures produced considerable amounts of low fatty acids and alcohols from sacchariferous substrates. In core-flooding tests, 43.0\u201353.5% of additional heavy oil was displaced by aerobic bacteria, producing biosurfactants, and 13.4\u201345.5% of oil was displaced by fermentative bacteria, producing low fatty acids, alcohols, and gas. A total of 1250 t additional oil was recovered as a result of the application of an MEOR technique at the Cheremukhovskoe heavy oil reservoir and Vostochno-Anzirskoe reservoir with light conventional oil.<\/jats:p>","DOI":"10.3390\/su12010015","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T03:15:01Z","timestamp":1577070901000},"page":"15","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6586-2467","authenticated-orcid":false,"given":"Tamara","family":"Nazina","sequence":"first","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diyana","family":"Sokolova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1358-5146","authenticated-orcid":false,"given":"Denis","family":"Grouzdev","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/1, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Semenova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Babich","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salimat","family":"Bidzhieva","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitriy","family":"Serdukov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitriy","family":"Volkov","sequence":"additional","affiliation":[{"name":"RITEK Co., Bolshaya Ordynka, 3, Moscow 115035, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Bugaev","sequence":"additional","affiliation":[{"name":"RITEK Co., Bolshaya Ordynka, 3, Moscow 115035, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2936-4265","authenticated-orcid":false,"given":"Alexey","family":"Ershov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marat","family":"Khisametdinov","sequence":"additional","affiliation":[{"name":"Tatar Oil Research and Design Institute, M. Djalil, 32, Bugulma 423236, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Borzenkov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7\/2, Moscow 117312, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1023\/B:MICI.0000044250.21076.0e","article-title":"Use of microorganisms in the biotechnology for the enhancement of oil recovery","volume":"73","author":"Belyaev","year":"2004","journal-title":"Microbiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0065-2164(08)00806-X","article-title":"Microbial processes in oil fields: Culprits, problems and opportunities","volume":"66","author":"Youssef","year":"2009","journal-title":"Adv. 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