{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:54:58Z","timestamp":1791489298093,"version":"4.3.4"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41402002"],"award-info":[{"award-number":["41402002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020JJ5481"],"award-info":[{"award-number":["2020JJ5481"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Funding of Hunan Province, China","award":["41402002"],"award-info":[{"award-number":["41402002"]}]},{"name":"Natural Science Funding of Hunan Province, China","award":["2020JJ5481"],"award-info":[{"award-number":["2020JJ5481"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The development of uranium mines has been necessary to obtain abundant and scarce uranium resources, but they also bring inevitable radioactive contamination to the surrounding soil, rivers and lakes. This paper explores the sensitivity of Cypridopsis vidua to the radioactive element uranium and the heavy elements cadmium and copper with single and combined acute toxicity experiments and combined toxicity model predictions. The results from the single toxicity experiments showed that the degree of toxic effects was cadmium &gt; copper &gt; uranium. The combined toxicity experiments showed that the compound toxicity of U-Cd and U-Cu was higher than the weakest component and lower than the strongest component, whereas the compound toxicity of Cd-Cu was higher than either of its components. When the overall proportion of a more toxic metal was increased, its mixed toxicity also increased, and vice versa. Combined toxicity predictions showed that the U-Cd combination was best described by the concentration additive (CA) model, the independent action (IA) model was more applicable to the Cd-Cu combination, and the most applicable model for the U-Cu combination changed depending on the concentration gradient. The acute toxicity data from this study provide a reference for the development of wastewater discharge standards for uranium mines, enriches the data related to the toxicity of uranium for ostracods and deepens the understanding of the threat of uranium pollution to aquatic ecosystems.<\/jats:p>","DOI":"10.3390\/toxics10070349","type":"journal-article","created":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T22:50:23Z","timestamp":1656283823000},"page":"349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sensitivity of Ostracods to U, Cd and Cu: The Case of Cypridopsis vidua"],"prefix":"10.3390","volume":"10","author":[{"given":"Liang","family":"Chen","sequence":"first","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"},{"name":"Hunan Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0549-441X","authenticated-orcid":false,"given":"Zheng","family":"Huo","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Su","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Province Engineering Technology Research Centre of Uranium Tailings Treatment Technology, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9718-2223","authenticated-orcid":false,"given":"Zhihua","family":"Su","sequence":"additional","affiliation":[{"name":"School of Management Science, Guizhou University of Finance and Economics, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"He","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"},{"name":"Hunan Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglin","family":"Sui","sequence":"additional","affiliation":[{"name":"School of Resource Environment and Safety Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"ref_1","first-page":"156","article-title":"Pollution Characteristics and Environmental Availability of Uranium in the Soils around A Uranium Waste Rock Pile in Guangdong Province, China","volume":"24","author":"Zhang","year":"2015","journal-title":"Ecol. Environ."},{"key":"ref_2","first-page":"149","article-title":"Progress in Remediation Technologies for Contaminated Groundwater from In-situ Leach Uranium Mining","volume":"41","author":"Chen","year":"2021","journal-title":"Min. Res. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.gexplo.2019.03.020","article-title":"Assessment of metal and metalloid contamination in the waters and stream sediments around the abandoned uranium mine area from Mort\u00f3rios, central Portugal","volume":"202","author":"Neiva","year":"2019","journal-title":"J. Geochem. Explor."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1002\/etc.5103","article-title":"Assessing the Toxicity of Mine-Water Mixtures and the Effectiveness of Water Quality Guideline Values in Protecting Local Aquatic Species","volume":"40","author":"Trenfield","year":"2021","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yi, L., Gao, B., Liu, H., Zhang, Y., Du, C., and Li, Y. (2020). Characteristics and Assessment of Toxic Metal Contamination in Surface Water and Sediments Near a Uranium Mining Area. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17020548"},{"key":"ref_6","first-page":"2784","article-title":"Pollution characteristics and risk assessment of heavy metals in sediment from Taojiang River Basin","volume":"39","author":"Chen","year":"2020","journal-title":"Environ. Chem."},{"key":"ref_7","first-page":"95","article-title":"Ecological Risk Assessment of Heavy Metals in the Surface Sediments in Dagu River","volume":"5","author":"Sun","year":"2020","journal-title":"Trans. Oceanol. Limnol."},{"key":"ref_8","first-page":"76","article-title":"Potential Ecological Risk Assessment of Heavy Metals Contamination in Farmland Soils near an Uranium Tailings Pond","volume":"32","author":"Zhang","year":"2016","journal-title":"Environ. Monit. China"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1097\/HP.0000000000001041","article-title":"Biota Dose Assessment of Small Rodents Sampled Near Breccia Pipe Uranium Mines in the Grand Canyon Watershed","volume":"117","author":"Minter","year":"2019","journal-title":"Health Phys."},{"key":"ref_10","first-page":"203","article-title":"Study on the Biological Toxicity Assessment of one Southern Uranium Mine","volume":"13","author":"Liu","year":"2018","journal-title":"Asian J. Ecotoxicol."},{"key":"ref_11","first-page":"150","article-title":"New Advances of study on monitoring environmental pollution by biomarkers","volume":"37","author":"Jiang","year":"2010","journal-title":"Guangdong Chem. Ind."},{"key":"ref_12","first-page":"190","article-title":"Advances on species diversity and ecology of marine pelagic ostracods","volume":"38","author":"Liao","year":"2019","journal-title":"Ecol. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.ecoenv.2018.08.095","article-title":"Carcinogenic profile, toxicity and source apportionment of polycyclic aromatic hydrocarbons accumulated from urban road dust in Tokyo, Japan","volume":"165","author":"Khanal","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.ecoenv.2017.05.011","article-title":"Development of a chronic sediment toxicity test using the benthic ostracod Heterocypris incongruens and their application to toxicity assessments of urban road dust","volume":"143","author":"Niyommaneerat","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1051\/e3sconf\/20184400174","article-title":"Assessment of water quality contaminated with arsenic using diatoms and Ostracodtoxkit F test","volume":"44","author":"Szmigielska","year":"2018","journal-title":"E3S Web Conf."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10661-010-1474-8","article-title":"Determination of EC50 toxicity data of selected heavy metals toward Heterocypris incongruens and their comparison to \u201cdirect-contact\u201d and microbiotests","volume":"174","author":"Wolska","year":"2011","journal-title":"Environ. Monit. Assess."},{"key":"ref_17","first-page":"27","article-title":"The application of OSTRACODTOXKIT F\u2122 test to assess metals contamination in road dust in Wroc\u0142aw agglomeration","volume":"29","author":"Rybak","year":"2020","journal-title":"Sci. Rev. Eng. Environ. Sci."},{"key":"ref_18","unstructured":"Lima, J.C.D.S., Moreira, R.A., Neto, A.J.G., de P\u00e1dua Andrade, D., Freitas, E.C., Daam, M.A., and Rocha, O. (2021). Metal Toxicity Can Affect Dragonfly Nymphs and Ostracods Predation Rates and Food Selectivity: Ecological Implications on Food Webs. Water Air Soil Pollut., 288."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1080\/15320383.2020.1835822","article-title":"Sediment Heavy Metal Contents, Ostracod Toxicity and Risk Assessment in Tropical Freshwater Lakes, Tamil Nadu, India","volume":"30","author":"Sivalingam","year":"2021","journal-title":"Soil Sediment Contam. Int. J."},{"key":"ref_20","first-page":"93","article-title":"Effect of industrial effluents on Cypridopsis species (Ostracoda: Cyprididae) diversity in River Cauvery, Tamilnadu, India","volume":"27","author":"Anandakumar","year":"2021","journal-title":"Ecol. Environ. Conserv."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Tan, C.W.J., Gouramanis, C., Pham, T.D., Hoang, D.Q., and Switzer, A.D. (2021). Ostracods as pollution indicators in Lap An Lagoon, central Vietnam. Environ. Pollut.","DOI":"10.1016\/j.envpol.2021.116762"},{"key":"ref_22","unstructured":"(2002). Surface Water Environmental Quality Standards. State Environmental Protection Administration (Standard No. GB 3838-2002)."},{"key":"ref_23","unstructured":"Anzecc, A. (2008). Australian and New Zealand Guidelines for Fresh and Marine Water Quality, Australian and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council of Australia and New Zealand."},{"key":"ref_24","unstructured":"Proykova, A., Kraetke, R., Bertollini, R., Borges, T., Duarte-Davidson, R., Panagiotakos, D., Slama, R., Scott, M., Testai, E., and Vermeire, T. (2018). Memorandum on weight of evidence and uncertainties Revision 2018, European Commission."},{"key":"ref_25","first-page":"143","article-title":"Characteristics of Distribution and Ecology of Recent Ostracods from Dongting Lake","volume":"37","author":"Chen","year":"2019","journal-title":"Acta Sedimentol. Sin."},{"key":"ref_26","first-page":"280","article-title":"New record of freshwater ostracods from the Yuandang Lake in the east China","volume":"33","author":"Yao","year":"2016","journal-title":"Acta Micropalaeontol. Sin."},{"key":"ref_27","first-page":"106","article-title":"Freshwater ostracods at the DT01 section from the Dongting Lake Hunan province, China","volume":"35","author":"Chen","year":"2018","journal-title":"Acta Micropalaeontol. Sin."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.4028\/www.scientific.net\/AMM.361-363.1054","article-title":"Mathematical Derivation of Concentration Addition, Independent Action and Effect Summation Models","volume":"361\u2013363","author":"Ge","year":"2013","journal-title":"Appl. Mech. Mater."},{"key":"ref_29","first-page":"91","article-title":"Progress on CA and IA models in combined toxicity prediction of chemical mixtures","volume":"41","author":"Xu","year":"2020","journal-title":"Prog. Vet. Med."},{"key":"ref_30","first-page":"118","article-title":"Acutetoxicity experiment of Cd2+, Zn2+ and Cu2+ in physocypria kraepelini (ostracoda)","volume":"27","author":"Chen","year":"2010","journal-title":"Acta Micropalaeontol. Sin."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.tox.2010.12.007","article-title":"A novel model integrated concentration addition with independent action for the prediction of toxicity of multi-component mixture","volume":"280","author":"Qin","year":"2011","journal-title":"Toxicology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1007\/s11356-010-0419-7","article-title":"A novel direct equipartition ray design (EquRay) procedure for toxicity interaction between ionic liquid and dichlorvos","volume":"18","author":"Dou","year":"2011","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/j.1744-7348.1939.tb06990.x","article-title":"The Toxicity of Poisons Applied Jointly","volume":"26","author":"Bliss","year":"1939","journal-title":"Ann. Appl. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.chemosphere.2016.08.064","article-title":"Mixture cytotoxicity assessment of ionic liquids and heavy metals in MCF-7 cells using mixtox","volume":"163","author":"Zhu","year":"2016","journal-title":"Chemosphere"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ecoenv.2012.11.022","article-title":"Modeling non-monotonic dose-response relationships: Model evaluation and hormetic quantities exploration","volume":"89","author":"Zhu","year":"2013","journal-title":"Ecotox. Environ. Safe."},{"key":"ref_36","first-page":"113","article-title":"Comparision between two confidence intervals of dose-response relationships","volume":"29","author":"Zhu","year":"2009","journal-title":"China Environ. Sci."},{"key":"ref_37","unstructured":"UN GHS (2013). Globally harmonized system of classification and labelling of chemicals (GHS), United Nation. [5th revised ed.]."},{"key":"ref_38","first-page":"3761","article-title":"Developmental toxicity in zebrafish (Danio rerio) exposed to uranium: A comparison with lead, cadmium, and iron","volume":"97","author":"Shankar","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1002\/ajim.20289","article-title":"Leukemia and exposure to ionizing radiation among German uranium miners","volume":"49","author":"Lindtner","year":"2006","journal-title":"Am. J. Ind. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jenvrad.2006.01.008","article-title":"Assessment of radiation exposure in the uranium mining and milling area of Mailuu Suu, Kyrgyzstan","volume":"88","author":"Vandenhove","year":"2006","journal-title":"J. Environ. Radioact."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2174\/1871528114666160105112441","article-title":"A Systematic Review of the Molecular Mechanisms of Uranium-Induced Reproductive Toxicity","volume":"14","author":"Asghari","year":"2015","journal-title":"Inflamm. Allergy-Drug Targets"},{"key":"ref_42","unstructured":"Yin, J. (2014). Studies on Toxics Effect and Mechanism of Heavy Metals on Zebrafish, Peking Union Medical College. Available online: https:\/\/kns.cnki.net\/KCMS\/detail\/detail.aspx?dbname=CDFD1214&filename=1014345479.nh."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1002\/etc.3342","article-title":"A test of the additivity of acute toxicity of binary-metal mixtures of Ni with Cd, Cu, and Zn to Daphnia magna, using the inflection point of the concentration-response curves","volume":"35","author":"Traudt","year":"2016","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_44","first-page":"10","article-title":"Single and joint toxicity of Cu2+, Cd2+ and Cr6+ on Poecilia reticulata","volume":"3","author":"Zhao","year":"2009","journal-title":"Water Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/S0147-6513(02)00032-5","article-title":"Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotonus fuscatus var radula (L.)","volume":"53","author":"Otitoloju","year":"2002","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.chemosphere.2005.04.089","article-title":"Toxicity of uranium and copper individually, and in combination, to a tropical freshwater macrophyte (Lemna aequinoctialis)","volume":"62","author":"Charles","year":"2006","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Xu, C., Li, T., Hu, C., Guo, H., Ye, J., Li, L., Liu, W., and Niu, L. (2021). Waterborne uranium causes toxic effect and thyroid disruption in zebrafish larvae. Ecotoxicol. Environ. Saf., 208.","DOI":"10.1016\/j.ecoenv.2020.111585"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.aquatox.2011.07.010","article-title":"Metal uptake and acute toxicity in zebrafish: Common mechanisms across multiple metals","volume":"105","author":"Alsop","year":"2011","journal-title":"Aquat. Toxicol."},{"key":"ref_49","first-page":"1","article-title":"A compilation of data on the toxicity of chemicals to species in Australasia. Part 3: Metals","volume":"8","author":"Markich","year":"2002","journal-title":"Australas. J. Ecotoxicol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.ecoenv.2011.02.014","article-title":"Toxicity of uranium, molybdenum, nickel, and arsenic to Hyalella azteca and Chironomus dilutus in water-only and spiked-sediment toxicity tests","volume":"74","author":"Liber","year":"2011","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_51","first-page":"136104","article-title":"Toxicity of Metals to a Freshwater Ostracod: Stenocypris major","volume":"2011","author":"Yakub","year":"2011","journal-title":"J. Toxicol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.chemosphere.2018.12.148","article-title":"Population response of the estuarine copepod Eurytemora affinis to its bioaccumulation of trace metals","volume":"220","author":"Zidour","year":"2019","journal-title":"Chemosphere"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1002\/etc.4419","article-title":"Sensitivity of a Large and Representative Sample of Antarctic Marine Invertebrates to Metals","volume":"38","author":"Kefford","year":"2019","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Li, L., Tian, X., Yu, X., and Dong, S. (2016). Effects of Acute and Chronic Heavy Metal (Cu, Cd, and Zn) Exposure on Sea Cucumbers (Apostichopus japonicus). BioMed Res. Int., 2016.","DOI":"10.1155\/2016\/4532697"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.aquatox.2005.07.012","article-title":"Chronic toxicity of uranium to a tropical green alga (Chlorella sp.) in natural waters and the influence of dissolved organic carbon","volume":"75","author":"Hogan","year":"2005","journal-title":"Aquat. Toxicol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ecoenv.2015.04.023","article-title":"Nested interactions in the combined toxicity of uranium and cadmium to the nematode Caenorhabditis elegans","volume":"118","author":"Margerit","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/S0168-9452(01)00478-2","article-title":"Physiological responses of Lemna trisulca L. (duckweed) to cadmium and copper bioaccumulation","volume":"161","author":"Prasad","year":"2001","journal-title":"Plant Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1002\/etc.2787","article-title":"Acute toxicity of binary and ternary mixtures of Cd, Cu, and Zn to Daphnia magna","volume":"34","author":"Meyer","year":"2014","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/etc.2869","article-title":"Development and application of a multimetal multibiotic ligand model for assessing aquatic toxicity of metal mixtures","volume":"34","author":"Santore","year":"2015","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.ecoenv.2018.03.003","article-title":"Can chronic exposure to imidacloprid, clothianidin, and thiamethoxam mixtures exert greater than additive toxicity in Chironomus dilutus?","volume":"156","author":"Maloney","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1016\/j.ecoenv.2009.04.014","article-title":"Behavioural and developmental toxicity of chlorpyrifos and nickel chloride to zebrafish (Danio rerio) embryos and larvae","volume":"72","author":"Kienle","year":"2009","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s10646-017-1803-6","article-title":"Assessing the environmental hazard of individual and combined pharmaceuticals: Acute and chronic toxicity of fluoxetine and propranolol in the crustacean Daphnia magna","volume":"26","author":"Varano","year":"2017","journal-title":"Ecotoxicology"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.envpol.2018.11.106","article-title":"Phytotoxicity of individual and binary mixtures of rare earth elements (Y, La, and Ce) in relation to bioavailability","volume":"246","author":"Gong","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.1897\/04-431R.1","article-title":"Significance Testing of Synergistic\/Antagonistic, Dose Level-Dependent, or Dose Ratio-Dependent Effects in Mixture Dose-Response Analysis","volume":"24","author":"Jonker","year":"2005","journal-title":"Environ. Toxicol. Chem."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/7\/349\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:39:42Z","timestamp":1760139582000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/7\/349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,24]]},"references-count":64,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["toxics10070349"],"URL":"https:\/\/doi.org\/10.3390\/toxics10070349","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,24]]}}}