{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:53:50Z","timestamp":1788882830547,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T00:00:00Z","timestamp":1668729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Lublin, Al. Rac\u0142awickie 1, 20-059 Lublin, Poland","award":["DS466"],"award-info":[{"award-number":["DS466"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Postmortem carbon monoxide (CO) and hydrogen cyanide (HCN) diffusion under ambient conditions was assessed in a human cadaver model. The main objective of this study was to determine whether the postmortem diffusion of HCN and CO greatly affected the determination of HCN, carboxyhemoglobin (COHb), and carboxymyoglobin (COMb). Layered samples of blood, musculocutaneous, and muscular specimens were collected from the adult cadavers and placed in the tight chambers designed for the purpose of this experiment. The specimens were treated with CO and HCN for 24 h. COHb and COMb were determined using headspace gas chromatography (GC) with an O-FID detector while the HCN values were assessed using a GC headspace with an NPD detector. It was shown that the skin substantially limited the diffusion of CO which penetrated the superficial layers of the muscle very slightly, all the while not affecting the blood level of COHb in the 4.5 cm layer of the muscle located underneath. There were no differences regarding the CO diffusion between superficially charred and thermally coagulated compared to that observed in intact integuments. In addition, the cutaneous sample deprived of the adipose layer was not shown to be a barrier to the moderate diffusion of CO into the blood layer below. HCN was found to easily diffuse from the skin to the blood vessels (vein specimens), and partial charring and thermocoagulation of the superficial muscular layer favored the diffusion of cyanides into the tissues. Similarly to CO, HCN diffusion to the blood and muscles was greatly limited by the adipose layer.<\/jats:p>","DOI":"10.3390\/toxics10110707","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T04:33:32Z","timestamp":1669005212000},"page":"707","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Diffusion of Carbon Monoxide and Hydrogen Cyanide to Muscles and Blood\u2014An Experimental Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1372-8987","authenticated-orcid":false,"given":"Jacek","family":"Baj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7637-6387","authenticated-orcid":false,"given":"Grzegorz","family":"Buszewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7040-4754","authenticated-orcid":false,"given":"Dominika","family":"Przygodzka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-7627","authenticated-orcid":false,"given":"Alicja","family":"Forma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6881-3161","authenticated-orcid":false,"given":"Jolanta","family":"Flieger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4184-9305","authenticated-orcid":false,"given":"Grzegorz","family":"Teresi\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1080\/20024091064246","article-title":"Toxicity of fire smoke","volume":"4","author":"Alarie","year":"2002","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1081\/CLT-200035211","article-title":"Carbon Monoxide and Cyanide Poisoning in Fire Related Deaths in Victoria, Australia","volume":"42","author":"Yeoh","year":"2004","journal-title":"J. Toxicol. Clin. Tox."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0736-4679(84)90078-7","article-title":"Carbon monoxide poisoning: Mechanisms, presentation, and controversies in management","volume":"1","author":"Olson","year":"1984","journal-title":"J. Emerg. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0300-483X(03)00005-2","article-title":"The clinical toxicology of carbon monoxide","volume":"187","author":"Gorman","year":"2003","journal-title":"Toxicology"},{"key":"ref_5","first-page":"160","article-title":"Hydrogen cyanide in fire smoke: An underappreciated threat","volume":"33","author":"Walsh","year":"2004","journal-title":"Emerg. Med. Serv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.aca.2010.05.042","article-title":"Recent developments in cyanide detection: A review","volume":"673","author":"Ma","year":"2010","journal-title":"Anal. Chim. Acta."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1093\/clinchem\/39.8.1693","article-title":"Measurement of carboxyhemoglobin and total hemoglobin by five specialized spectrophotometers (CO-oximeters) in comparison with reference methods","volume":"39","author":"Mahoney","year":"1993","journal-title":"Clin Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/10915810591007256","article-title":"Evaluation of the Methods Used for Carboxyhemoglobin Analysis in Postmortem Blood","volume":"24","author":"Boumba","year":"2005","journal-title":"Int. J. Toxicol."},{"key":"ref_9","first-page":"76","article-title":"Determination of carboxymyoglobin in cardiac and femoral muscles by means of headspace gas chromatography","volume":"44","author":"Buszewicz","year":"2000","journal-title":"Probl. Forensic Sci."},{"key":"ref_10","first-page":"21","article-title":"Rapid Determination of Carboxyhemoglobin in Postmortem Blood using Fully-Automated Headspace Gas Chromatography with Methaniser and FID","volume":"4","author":"Walch","year":"2010","journal-title":"Open Tox. J."},{"key":"ref_11","first-page":"132","article-title":"Chromatographic determination of carbon monoxide and carboxyhaemoglobin by head-space technique using a catalytic microreactor with FID detector","volume":"36","author":"Buszewicz","year":"1997","journal-title":"Probl. Forensic Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1001\/jama.2013.43127","article-title":"Diffusion of Carbon Monoxide Through Gypsum Wallboard","volume":"7","author":"Hampson","year":"2013","journal-title":"JAMA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.meatsci.2016.06.013","article-title":"Carbon monoxide concentration and exposure time effects on the depth of CO penetration and surface color of raw and cooked beef longissimus lumborum steaks","volume":"121","author":"Sakowska","year":"2016","journal-title":"Meat Sci."},{"key":"ref_14","first-page":"486","article-title":"Der Wert der Quantitativen CO-Bestimmung f\u00fcr die Kriminalistik und Klinik der CO-Vergiftung","volume":"21","author":"Breitenecker","year":"1939","journal-title":"Wiener Klin. Woch."},{"key":"ref_15","unstructured":"Hofmann, E. (1908). Atlas und Grundriss der Gerichtlichen Medizin, JF Lehmann\u2019s Verlag. (In German)."},{"key":"ref_16","unstructured":"Reimann, W., and Prokop, O. (1973). Vademecum Gerichtsmedizin, VEB Verlag Volk. (In German)."},{"key":"ref_17","unstructured":"Glaister, J. (1953). Medical Jurisprudence and Toxicology, E & S Livingstone Ltd.. [9th ed.]."},{"key":"ref_18","first-page":"223","article-title":"Experimentelles zur Lehre von der Kohlenoxydvergiftung","volume":"23","author":"Wachholz","year":"1902","journal-title":"Vjschr. Gerichtl. Med."},{"key":"ref_19","first-page":"149","article-title":"Przyczynki do\u015bwiadczalne do nauki o otruciu tlenkiem w\u0119gla","volume":"14","author":"Wachholz","year":"1902","journal-title":"Now. Lekarskie."},{"key":"ref_20","first-page":"1233","article-title":"Untersuchungen zur Kohlenoxydvergiftung","volume":"48","author":"Strassmann","year":"1904","journal-title":"Berl. Klin Wochenschr"},{"key":"ref_21","first-page":"46","article-title":"Untersuchungen \u00fcber postmortales Eindringen von Kohlenoxyd in den K\u00f6rper","volume":"38","author":"Stoll","year":"1909","journal-title":"Vjschr. Gerichtl. Med."},{"key":"ref_22","unstructured":"(1938). Reuter: Abderhaldens Handbuch der Biologischen Arbeitsmethoden, Urban & Schwarzenberg. (In German)."},{"key":"ref_23","first-page":"411","article-title":"Die Anwendung der Spektrophotometrischen Blutuntersuchung in der gerichtlichen Medizin","volume":"2","author":"Schwarzacher","year":"1923","journal-title":"Dtsch. Z Gerichtl. Med."},{"key":"ref_24","unstructured":"National Fire Protection Association (NFPA) (2022, October 19). Fire Loss in the United States during 2021 Shelby Hall and Ben Evarts September 2022. Available online: https:\/\/www.nfpa.org."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1097\/MEJ.0b013e328357170b","article-title":"Cyanide poisoning by fire smoke inhalation: A European expert consensus","volume":"20","author":"Anseeuw","year":"2013","journal-title":"Eur. J. Emerg. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S1344-6223(03)00078-6","article-title":"Lethal cyanide inhalation with post-mortem trans-cutaneous cyanide diffusion","volume":"5","author":"Seidl","year":"2003","journal-title":"Leg. Med."},{"key":"ref_27","unstructured":"Ballantyne, B., and Marrs, T.C. (1987). Clinical and Experimental Toxicology of Cyanides, Wright Bristol: IOP Publishing."},{"key":"ref_28","first-page":"1","article-title":"Hydrocyanic acid gas poisoning by absorption through the skin","volume":"14","author":"Drinker","year":"1932","journal-title":"J. Ind. Hyg."},{"key":"ref_29","first-page":"125","article-title":"The successful treatment of two recent cases of cyanide poisoning","volume":"7","author":"Potter","year":"1950","journal-title":"Br. J. Ind. Med."},{"key":"ref_30","unstructured":"Petrova, F., and Fishbein, L. (2004). Concise International Chemical Assessment Document 61, WHO Library Cataloguing-in-Publication Data."},{"key":"ref_31","unstructured":"DiPalma, J.R. (1971). Dril\u2019s Pharmacol Med, McGraw-Hill. [4th ed.]."},{"key":"ref_32","unstructured":"Petrova Simeonova, F., and Fishbein, L. (2004). Hydrogen Cyanide and Cyanides: Human Health Aspects, World Health Organization. Concise International Chemical Assessment Document."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0379-0738(91)90077-V","article-title":"High cyanide level in a homicide victim burned after death: Evidence of post-mortem diffusion","volume":"49","author":"Karhunen","year":"1991","journal-title":"Forensic Sci. Int."},{"key":"ref_34","first-page":"82","article-title":"The role of endogenous hydrogen cyanide in forensic medical appraisal and interpretation of fire victims","volume":"54","author":"Grabowska","year":"2003","journal-title":"Probl. Forensic Sci."},{"key":"ref_35","unstructured":"Brauer, G. (1963). Handbook of Preparative Inorganic Chemistry, Academic Press."},{"key":"ref_36","first-page":"100","article-title":"The use of gas chromatography in chemical diagnosis of poisonings with cyanides","volume":"55","author":"Wachowiak","year":"2003","journal-title":"Probl. Forensic Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.0954-6820.1967.tb12633.x","article-title":"Endogenous carbon monoxide production and body CO stores","volume":"181","author":"Coburn","year":"1967","journal-title":"Acta Med. Scand."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1080\/00039896.1979.10667410","article-title":"Carbon monoxide: A hazard to fire fighters","volume":"34","author":"Barnard","year":"1979","journal-title":"Arch. Environ. Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1023\/A:1015366527753","article-title":"Carbon monoxide levels in structure fires: Effects of wood in the upper layer of a post-flashover compartment fire","volume":"34","author":"Lattimer","year":"1998","journal-title":"Fire Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/s00194-016-0090-5","article-title":"Hydrogen cyanide in fire tests","volume":"26","author":"Stoll","year":"2016","journal-title":"Rechtsmedizin"}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/11\/707\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:38Z","timestamp":1760145698000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/10\/11\/707"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,18]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["toxics10110707"],"URL":"https:\/\/doi.org\/10.3390\/toxics10110707","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,18]]}}}