{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:53:35Z","timestamp":1784325215242,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Catalina Ruiz research staff training aid program of the Regional Ministry of Economy, Knowledge, and Employment of the Canary Islands Government and the European Social Fund","award":["APCR2022010002"],"award-info":[{"award-number":["APCR2022010002"]}]},{"name":"Catalina Ruiz research staff training aid program of the Regional Ministry of Economy, Knowledge, and Employment of the Canary Islands Government and the European Social Fund","award":["APCR2022010003"],"award-info":[{"award-number":["APCR2022010003"]}]},{"name":"Catalina Ruiz research staff training aid program of the Regional Ministry of Economy, Knowledge, and Employment of the Canary Islands Government and the European Social Fund","award":["ULPGC-2021-2022"],"award-info":[{"award-number":["ULPGC-2021-2022"]}]},{"name":"a predoctoral grant to \u00c1lvaro Ramos-Luzardo from the University of Las Palmas de Gran Canaria","award":["APCR2022010002"],"award-info":[{"award-number":["APCR2022010002"]}]},{"name":"a predoctoral grant to \u00c1lvaro Ramos-Luzardo from the University of Las Palmas de Gran Canaria","award":["APCR2022010003"],"award-info":[{"award-number":["APCR2022010003"]}]},{"name":"a predoctoral grant to \u00c1lvaro Ramos-Luzardo from the University of Las Palmas de Gran Canaria","award":["ULPGC-2021-2022"],"award-info":[{"award-number":["ULPGC-2021-2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 \u00b5L of serum. Among them, 92% could be measured at concentrations below 1.25 ng\/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum.<\/jats:p>","DOI":"10.3390\/toxics11060498","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T02:39:47Z","timestamp":1685587187000},"page":"498","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2773-8075","authenticated-orcid":false,"given":"Cristian","family":"Rial-Berriel","sequence":"first","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9435-2228","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Ramos-Luzardo","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1272-0545","authenticated-orcid":false,"given":"Andrea","family":"Acosta-Dacal","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8554-5728","authenticated-orcid":false,"given":"Ana","family":"Mac\u00edas-Montes","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Fern\u00e1ndez-Valer\u00f3n","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3237-0316","authenticated-orcid":false,"given":"Luis Alberto","family":"Henr\u00edquez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"},{"name":"Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1534-7758","authenticated-orcid":false,"given":"Manuel","family":"Zumbado","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"},{"name":"Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0195-4565","authenticated-orcid":false,"given":"Luis D.","family":"Boada","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"},{"name":"Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio P.","family":"Luzardo","sequence":"additional","affiliation":[{"name":"Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), Universidad de Las Palmas de Gran Canaria, Paseo Blas Cabrera s\/n, 35016 Las Palmas de Gran Canaria, Spain"},{"name":"Spanish Biomedical Research Center in Physiopathology of Obesity and Nutrition (CIBERObN), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.envpol.2019.05.008","article-title":"An Efficient Method to Simultaneously Analyze Multi-Class Organic Pollutants in Human Serum","volume":"251","author":"Jia","year":"2019","journal-title":"Environ. 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