{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:01:58Z","timestamp":1787065318764,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82073524"],"award-info":[{"award-number":["82073524"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>(1) Objective: Rare earth neodymium oxide (Nd2O3) is refined and used extensively around the world, and the occupational and environmental safety of rare piles of the earth has attracted considerable attention. Nd2O3 enters the human body through the respiratory system, reaches various organs through blood circulation, and accumulates to produce toxic effects. At present, little is known about the reproductive toxicity of Nd2O3. Non-coding RNAs participate in a variety of physiological activities and are very important for spermatogenesis. However, it is unknown whether they are involved in Nd2O3-induced reproductive toxicity. Therefore, we conducted a pathological analysis, sperm quality testing, and RNA-seq on the testicular tissue of mice exposed to Nd2O3 to find the key genes and regulatory pathways of male reproductive damage and explore the early biomarkers and mechanisms of reproductive damage caused by Nd2O3. (2) Methods: After exposure of mice to Nd2O3, we carried out a pathological analysis and RNA-seq analysis for miRNAs\/lncRNAs\/circRNAs\/mRNAs on the testicular tissue of mice, and the total RNAs were used to investigate miRNA\/lncRNA\/circRNA\/mRNA expression profiles by strand-specific RNA sequencing at the transcriptome level to help uncover RNA-related mechanisms in Nd2O3-induced toxicity. (3) Results: Nd2O3 damaged testis and sperm morphology, significantly decreased the number of sperm, and deformed the sperm head and tail. RNA-seq analysis showed that the expression level of mRNA\/miRNA\/circRNA\/lncRNA in the testicular tissue of mice exposed to Nd2O3 is abnormal. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that the functional enrichment of differentially expressed genes (DEGs) and their target genes was closely related to the related pathway of spermatogenesis. Furthermore, some miRNAs\/lncRNAs\/circRNAs that were greatly upregulated or inducibly expressed, implying their potential value as candidate markers for Nd2O3-induced reproductive toxicity, help us to further investigate the mechanisms of key genes, key signaling pathways, and inter-gene regulation for Nd2O3-induced reproductive toxicity. (4) Conclusions: This study provides the first database of a Nd2O3-induced transcriptome. This information is useful for the development of biomarkers of Nd2O3-induced reproductive injury and promotes understanding of the reproductive toxicity mechanism of Nd2O3.<\/jats:p>","DOI":"10.3390\/toxics11120952","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T10:41:51Z","timestamp":1700649711000},"page":"952","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["RNA-Seq Analysis of Testes from Mice Exposed to Neodymium Oxide"],"prefix":"10.3390","volume":"11","author":[{"given":"Shurui","family":"Wang","sequence":"first","affiliation":[{"name":"Baotou Medical College, Baotou 014042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Bu","sequence":"additional","affiliation":[{"name":"Center for Global Health, The Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yudan","family":"Yun","sequence":"additional","affiliation":[{"name":"Baotou Medical College, Baotou 014042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Shi","sequence":"additional","affiliation":[{"name":"Baotou Medical College, Baotou 014042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Wulanchabu Medical College, Wulanchabu 012001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanrong","family":"Gao","sequence":"additional","affiliation":[{"name":"Baotou Medical College, Baotou 014042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1021\/es203518d","article-title":"Evaluating rare earth element availability: A case with revolutionary demand from clean technologies","volume":"46","author":"Alonso","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s12011-020-02165-0","article-title":"Bone Mineral Density in Population Long-Term Exposed to Rare Earth Elements from a Mining Area of China","volume":"199","author":"Liu","year":"2021","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"700398","DOI":"10.3389\/fgene.2021.700398","article-title":"Comprehensive Analysis of Differentially Expressed lncRNAs miRNAs and mRNA and Their ceRNA Network of Patients with Rare-Earth Pneumoconiosis","volume":"12","author":"Shi","year":"2021","journal-title":"Front. Genet."},{"key":"ref_4","first-page":"75","article-title":"Neodymium Oxide Induces Cytotoxicity and Activates NF-kappaB and Caspase-3 in NR8383 Cells","volume":"30","author":"Huang","year":"2017","journal-title":"Biomed. Environ. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e12920","DOI":"10.1111\/and.12920","article-title":"Cerium oxide nanoparticle elicits oxidative stress, endocrine imbalance and lowers sperm characteristics in testes of balb\/c mice","volume":"50","author":"Adebayo","year":"2018","journal-title":"Andrologia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"411","DOI":"10.2131\/jts.45.411","article-title":"Effects of intragastric administration of La2O3 nanoparticles on mouse testes","volume":"45","author":"Yuan","year":"2020","journal-title":"J. Toxicol. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Agarwal, A., Mulgund, A., Hamada, A., and Chyatte, M.R. (2015). A unique view on male infertility around the globe. Reprod. Biol. Endocrinol., 13.","DOI":"10.1186\/s12958-015-0032-1"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"109528","DOI":"10.1016\/j.envres.2020.109528","article-title":"Ambient air pollution and male fecundity: A retrospective analysis of longitudinal data from a Chinese human sperm bank (2013\u20132018)","volume":"186","author":"Qiu","year":"2020","journal-title":"Environ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1080\/09603123.2020.1870668","article-title":"Transgenerational male reproductive effect of prenatal arsenic exposure: Abnormal spermatogenesis with Igf2\/H19 epigenetic alteration in CD1 mouse","volume":"32","author":"Yin","year":"2022","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8276","DOI":"10.1038\/s41598-021-87677-y","article-title":"Transgenerational effects in DNA methylation, genotoxicity and reproductive phenotype by chronic arsenic exposure","volume":"11","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Joshi, M., and Rajender, S. (2020). Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility. Reprod. Biol. Endocrinol., 18.","DOI":"10.1186\/s12958-020-00660-6"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"665","DOI":"10.2174\/1389200220666190729130020","article-title":"Dysregulation of lncRNA and circRNA Expression in Mouse Testes after Exposure to Triptolide","volume":"20","author":"Xiong","year":"2019","journal-title":"Curr. Drug Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"106621","DOI":"10.1016\/j.anireprosci.2020.106621","article-title":"RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa","volume":"222","author":"Prakash","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9936","DOI":"10.1111\/jcmm.15593","article-title":"Applying high-throughput sequencing to identify and evaluate foetal chromosomal deletion and duplication","volume":"24","author":"Wu","year":"2020","journal-title":"J. Cell Mol. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.scitotenv.2018.04.235","article-title":"Sources, behaviour, and environmental and human health risks of high-technology rare earth elements as emerging contaminants","volume":"636","author":"Gwenzi","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.2147\/IJN.S230949","article-title":"La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2\/ARE Signaling Pathway in Kunming Mice","volume":"15","author":"Yuan","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1530\/REP-19-0139","article-title":"Dynamic paraspeckle component localisation during spermatogenesis","volume":"158","author":"Major","year":"2019","journal-title":"Reproduction"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1159\/000518960","article-title":"miR-27b-3p Improved High Glucose-Induced Spermatogenic Cell Damage via Regulating Gfpt1\/HBP Signaling","volume":"63","author":"Zheng","year":"2022","journal-title":"Eur. Surg. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"gaaa085","DOI":"10.1093\/molehr\/gaaa085","article-title":"The role of Wnt signaling in male reproductive physiology and pathology","volume":"27","author":"Xue","year":"2021","journal-title":"Mol. Hum. Reprod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3892\/etm.2016.3959","article-title":"Correlative study on the JAK-STAT\/PSMbeta3 signal transduction pathway in asthenozoospermia","volume":"13","author":"Li","year":"2017","journal-title":"Exp. Ther. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"100886","DOI":"10.1016\/j.mam.2020.100886","article-title":"New perspectives on PPAR, VDR and FXRalpha as new actors in testicular pathophysiology","volume":"78","author":"Monrose","year":"2021","journal-title":"Mol. Aspects Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1002\/tox.22866","article-title":"Di-2-ethylhexyl phthalate (DEHP) induces apoptosis and autophagy of mouse GC-1 spg cells","volume":"35","author":"Gan","year":"2020","journal-title":"Environ. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Shen, J., Yang, D., Zhou, X., Wang, Y., Tang, S., Yin, H., Wang, J., Chen, R., and Chen, J. (2019). Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20164042"},{"key":"ref_24","first-page":"375","article-title":"The Expression of TLR2 and TLR3 in Sertoli Cells of Azoospermic Patients","volume":"19","author":"Lakpour","year":"2017","journal-title":"Cell J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"120064","DOI":"10.1016\/j.lfs.2021.120064","article-title":"The protective effect of quercetin on macrophage pyroptosis via TLR2\/Myd88\/NF-kappaB and ROS\/AMPK pathway","volume":"291","author":"Luo","year":"2022","journal-title":"Life Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.stem.2018.08.007","article-title":"Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis","volume":"23","author":"Wang","year":"2018","journal-title":"Cell Stem Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19089","DOI":"10.1038\/s41598-021-98267-3","article-title":"Integration and gene co-expression network analysis of scRNA-seq transcriptomes reveal heterogeneity and key functional genes in human spermatogenesis","volume":"11","author":"Salehi","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/11\/12\/952\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:27:21Z","timestamp":1760131641000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/11\/12\/952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,22]]},"references-count":27,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["toxics11120952"],"URL":"https:\/\/doi.org\/10.3390\/toxics11120952","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,22]]}}}