{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:14:08Z","timestamp":1792120448385,"version":"4.4.0"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01-CACA211875"],"award-info":[{"award-number":["R01-CACA211875"]}]},{"name":"National Institutes of Health","award":["W81XWH2110903"],"award-info":[{"award-number":["W81XWH2110903"]}]},{"name":"National Institutes of Health","award":["BC221269"],"award-info":[{"award-number":["BC221269"]}]},{"name":"DOD PRCRP Impact Award","award":["R01-CACA211875"],"award-info":[{"award-number":["R01-CACA211875"]}]},{"name":"DOD PRCRP Impact Award","award":["W81XWH2110903"],"award-info":[{"award-number":["W81XWH2110903"]}]},{"name":"DOD PRCRP Impact Award","award":["BC221269"],"award-info":[{"award-number":["BC221269"]}]},{"DOI":"10.13039\/100000005","name":"DOD Breakthrough Award","doi-asserted-by":"publisher","award":["R01-CACA211875"],"award-info":[{"award-number":["R01-CACA211875"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"DOD Breakthrough Award","doi-asserted-by":"publisher","award":["W81XWH2110903"],"award-info":[{"award-number":["W81XWH2110903"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"DOD Breakthrough Award","doi-asserted-by":"publisher","award":["BC221269"],"award-info":[{"award-number":["BC221269"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Environmental Toxicology Fellowship","award":["R01-CACA211875"],"award-info":[{"award-number":["R01-CACA211875"]}]},{"name":"Environmental Toxicology Fellowship","award":["W81XWH2110903"],"award-info":[{"award-number":["W81XWH2110903"]}]},{"name":"Environmental Toxicology Fellowship","award":["BC221269"],"award-info":[{"award-number":["BC221269"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The potential effects of poly- and perfluoroalkyl substances (PFAS) are a recently emergent human and environmental health concern. There is a consistent link between PFAS exposure and cancer, but the mechanisms are poorly understood. Although epidemiological evidence supporting PFAS exposure and cancer in general is conflicting, there is relatively strong evidence linking PFAS and testicular germ cell tumors (TGCTs). However, no mechanistic studies have been performed to date concerning PFAS and TGCTs. In this report, the effects of the legacy PFAS perfluorooctanesulfonic acid (PFOS) and the newer \u201cclean energy\u201d PFAS lithium bis(trifluoromethylsulfonyl)imide (LiTFSi, called HQ-115), on the tumorigenicity of TGCTs in mice, TGCT cell survival, and metabolite production, as well as gene regulation were investigated. In vitro, the proliferation and survival of both chemo-sensitive and -resistant TGCT cells were minimally affected by a wide range of PFOS and HQ-115 concentrations. However, both chemicals promoted the growth of TGCT cells in mouse xenografts at doses consistent with human exposure but induced minimal acute toxicity, as assessed by total body, kidney, and testis weight. PFOS, but not HQ-115, increased liver weight. Transcriptomic alterations of PFOS-exposed normal mouse testes were dominated by cancer-related pathways and gene expression alterations associated with the H3K27me3 polycomb pathway and DNA methylation, epigenetic pathways that were previously showed to be critical for the survival of TGCT cells after cisplatin-based chemotherapy. Similar patterns of PFOS-mediated gene expression occurred in PFOS-exposed cells in vitro. Metabolomic studies revealed that PFOS also altered metabolites associated with steroid biosynthesis and fatty acid metabolism in TGCT cells, consistent with the proposed ability of PFAS to mimic fatty acid-based ligands controlling lipid metabolism and the proposed role of PFAS as endocrine disrupters. Our data, is the first cell and animal based study on PFAS in TGCTs, support a pro-tumorigenic effect of PFAS on TGCT biology and suggests epigenetic, metabolic, and endocrine disruption as potential mechanisms of action that are consistent with the non-mutagenic nature of the PFAS class.<\/jats:p>","DOI":"10.3390\/toxics12040232","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T10:03:59Z","timestamp":1711101839000},"page":"232","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Perfluorooctanesulfonic Acid Alters Pro-Cancer Phenotypes and Metabolic and Transcriptional Signatures in Testicular Germ Cell Tumors"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7494-1220","authenticated-orcid":false,"given":"Raya I.","family":"Boyd","sequence":"first","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1349-8381","authenticated-orcid":false,"given":"Doha","family":"Shokry","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0111-7668","authenticated-orcid":false,"given":"Zeeshan","family":"Fazal","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brayden C.","family":"Rennels","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Freemantle","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2269-1688","authenticated-orcid":false,"given":"Michael R.","family":"La Frano","sequence":"additional","affiliation":[{"name":"Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail S.","family":"Prins","sequence":"additional","affiliation":[{"name":"Departments of Urology, Pathology and Physiology, College of Medicine and Chicago Center for Health and Environment, University of Illinois Chicago, Chicago, IL 60612, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2607-1643","authenticated-orcid":false,"given":"Zeynep","family":"Madak Erdogan","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Division of Nutritional Sciences, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Cancer Center of Illinois, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0630-1520","authenticated-orcid":false,"given":"Joseph","family":"Irudayaraj","sequence":"additional","affiliation":[{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Cancer Center of Illinois, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3226-3847","authenticated-orcid":false,"given":"Ratnakar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Spinella","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Illinois Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, IL 61801, USA"},{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Cancer Center of Illinois, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA"},{"name":"Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,22]]},"reference":[{"key":"ref_1","unstructured":"Interstate Technology and Regulatory Council (2022, December 10). PFAS Technical and Regulatory Guidance Document and Fact Sheets. Available online: https:\/\/pfas-1.itrcweb.org\/wp-content\/uploads\/2022\/09\/PFAS-Guidance-Document-9-2022.pdf."},{"key":"ref_2","unstructured":"Organization for Economic Co-Operation and Development (2023, December 10). 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