{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T13:02:07Z","timestamp":1777986127623,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800400"],"award-info":[{"award-number":["2023YFD1800400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["SJCX19-0129"],"award-info":[{"award-number":["SJCX19-0129"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["2023YFD1800400"],"award-info":[{"award-number":["2023YFD1800400"]}]},{"name":"Postgraduate Research &amp; Practice Innovation Program of Jiangsu Province","award":["SJCX19-0129"],"award-info":[{"award-number":["SJCX19-0129"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Antibiotic resistance genes (ARGs) are emerging as significant environmental contaminants, posing potential health risks worldwide. Intensive livestock farming, particularly swine production, is a primary contributor to the escalation of ARG pollution. In this study, we employed metagenomic sequencing and quantitative polymerase chain reaction to analyze the composition of microorganisms and ARGs across four vectors in a typical swine fattening facility: dung, soil, airborne particulate matter (PM), and fodder. Surprisingly, soil and PM harbored a higher abundance of microorganisms and ARGs than dung. At the same time, fodder was more likely to carry eukaryotes. Proteobacteria exhibited the highest propensity for carrying ARGs, with proportions 9\u201320 times greater than other microorganisms. Furthermore, a strong interrelation among various ARGs was observed, suggesting the potential for cooperative transmission mechanisms. These findings underscore the importance of recognizing soil and PM as significant reservoirs of ARGs in swine facilities alongside dung. Consequently, targeted measures should be implemented to mitigate their proliferation, mainly focusing on airborne PM, which can rapidly disseminate via air currents. Proteobacteria, given their remarkable carrying capacity for ARGs with the primary resistance mechanism of efflux, represent a promising avenue for developing novel control strategies against antibiotic resistance.<\/jats:p>","DOI":"10.3390\/toxics12120916","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T11:17:22Z","timestamp":1734520642000},"page":"916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Contamination Characteristics of Antibiotic Resistance Genes in Multi-Vector Environment in Typical Regional Fattening House"],"prefix":"10.3390","volume":"12","author":[{"given":"Kai","family":"Wang","sequence":"first","affiliation":[{"name":"Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Shen","sequence":"additional","affiliation":[{"name":"Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhendong","family":"Guo","sequence":"additional","affiliation":[{"name":"Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130117, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuming","family":"Zhong","sequence":"additional","affiliation":[{"name":"Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113324","DOI":"10.1016\/j.jenvman.2021.113324","article-title":"Biogas energy generated from livestock manure in China: Current situation and future trends","volume":"297","author":"Wang","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1073\/pnas.1222743110","article-title":"Diverse and abundant antibiotic resistance genes in Chinese swine farms","volume":"110","author":"Zhu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.scitotenv.2013.06.069","article-title":"Assessing the biosafety risks of pig manure for use as a feedstock for composting","volume":"463\u2013464","author":"McCarthy","year":"2013","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"134921","DOI":"10.1016\/j.scitotenv.2019.134921","article-title":"Seasonal variations of microbial assemblage in fine particulate matter from a nursery pig house","volume":"708","author":"Tang","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_5","first-page":"397","article-title":"Antibiotic use in farming set to soar despite drug-resistance fears","volume":"614","author":"Reardon","year":"2023","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112715","DOI":"10.1016\/j.envres.2022.112715","article-title":"Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries","volume":"208","author":"Luiken","year":"2022","journal-title":"Environ. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"108074","DOI":"10.1016\/j.envint.2023.108074","article-title":"Characteristics of aerosols from swine farms: A review of the past two-decade progress","volume":"178","author":"Liu","year":"2023","journal-title":"Environ. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/978-1-0716-3072-3_15","article-title":"Tracking Antibiotic Resistance from the Environment to Human Health","volume":"2649","author":"Abdelrazik","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"43093","DOI":"10.1007\/s11356-020-10266-5","article-title":"The presence of tetracyclines and sulfonamides in swine feeds and feces: Dependence on the antibiotic type and swine growth stages","volume":"27","author":"Xu","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"149205","DOI":"10.1016\/j.scitotenv.2021.149205","article-title":"A systematic review on antibiotics misuse in livestock and aquaculture and regulation implications in China","volume":"798","author":"Shao","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_11","first-page":"269","article-title":"Spatial-temporal characteristics and decoupling effect of carbon emissions in the major pig producing areas in China","volume":"35","year":"2018","journal-title":"J. Agric. Resour. Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"38084","DOI":"10.1007\/s11356-020-09922-7","article-title":"An analysis of the environmental efficiency of pig farms and its determinants\u2014A field study from China","volume":"27","author":"Yan","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129719","DOI":"10.1016\/j.jhazmat.2022.129719","article-title":"Distribution of antibiotic, heavy metals and antibiotic resistance genes in livestock and poultry feces from different scale of farms in Ningxia, China","volume":"440","author":"Peng","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107751","DOI":"10.1016\/j.envint.2023.107751","article-title":"Airborne bacterial community and antibiotic resistome in the swine farming environment: Metagenomic insights into livestock relevance, pathogen hosts and public risks","volume":"172","author":"Gao","year":"2023","journal-title":"Environ. Int."},{"key":"ref_15","unstructured":"(2017). Standardization Administration of the People\u2019s Republic of China. Hygienical Standard for Feeds (Standard No. GB 13078-2017)."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"116638","DOI":"10.1016\/j.jenvman.2022.116638","article-title":"Seasonal dissemination of antibiotic resistome from livestock farms to surrounding soil and air: Bacterial hosts and risks for human exposure","volume":"325","author":"Wang","year":"2023","journal-title":"J. Environ. Manag."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.envpol.2019.04.086","article-title":"Distribution and physicochemical properties of particulate matter in swine confinement barns","volume":"250","author":"Shen","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41579-021-00559-y","article-title":"Prevotella diversity, niches and interactions with the human host","volume":"19","author":"Tett","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.fbr.2019.12.003","article-title":"Mucoromycota: Going to the roots of plant-interacting fungi","volume":"34","author":"Bonfante","year":"2020","journal-title":"Fungal Biol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s13225-021-00497-3","article-title":"Species diversity of Basidiomycota","volume":"114","author":"He","year":"2022","journal-title":"Fungal Divers."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Challacombe, J.F., Hesse, C.N., Bramer, L.M., McCue, L.A., Lipton, M., Purvine, S., Nicora, C., Gallegos-Graves, V., Porras-Alfaro, A., and Kuske, C.R. (2019). Genomes and secretomes of Ascomycota fungi reveal diverse functions in plant biomass decomposition and pathogenesis. BMC Genom., 20.","DOI":"10.1186\/s12864-019-6358-x"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Oliveira, T.C., Cabral, J., Santana, L.R., Tavares, G.G., Santos, L., Paim, T.P., Muller, C., Silva, F.G., Costa, A.C., and Souchie, E.L. (2022). The arbuscular mycorrhizal fungus Rhizophagus clarus improves physiological tolerance to drought stress in soybean plants. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-13059-7"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"967271","DOI":"10.1155\/2010\/967271","article-title":"The discussion goes on: What is the role of Euryarchaeota in humans?","volume":"2010","author":"Horz","year":"2010","journal-title":"Archaea"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/s41577-019-0268-7","article-title":"Host-microbiota interactions in inflammatory bowel disease","volume":"20","author":"Caruso","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1038\/s41575-022-00673-z","article-title":"Methanogenic archaea in the human gastrointestinal tract","volume":"19","author":"Hoegenauer","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"124957","DOI":"10.1016\/j.jhazmat.2020.124957","article-title":"Pollution characteristics of livestock faeces and the key driver of the spread of antibiotic resistance genes","volume":"409","author":"Yue","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.envpol.2019.02.093","article-title":"Antibiotic resistance genes and bacterial communities in cornfield and pasture soils receiving swine and dairy manures","volume":"248","author":"Chen","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.mib.2014.05.019","article-title":"Impacts of antibiotic use in agriculture: What are the benefits and risks?","volume":"19","author":"Durso","year":"2014","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1039\/c3em30682h","article-title":"Use patterns, excretion masses and contamination profiles of antibiotics in a typical swine farm, south China","volume":"15","author":"Zhou","year":"2013","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1111\/j.1348-0421.1996.tb01125.x","article-title":"Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen, Pasteurella piscicida","volume":"40","author":"Kim","year":"1996","journal-title":"Microbiol. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yang, F., Han, B., Gu, Y., and Zhang, K. (2020). Swine liquid manure: A hotspot of mobile genetic elements and antibiotic resistance genes. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-72149-6"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"121557","DOI":"10.1016\/j.envpol.2023.121557","article-title":"Partitioning and migration of antibiotic resistance genes at soil-water-air interface mediated by plasmids","volume":"327","author":"Zhu","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"106559","DOI":"10.1016\/j.envint.2021.106559","article-title":"Bioaerosol is an important transmission route of antibiotic resistance genes in pig farms","volume":"154","author":"Song","year":"2021","journal-title":"Environ. Int."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zhu, L., Yan, Z., Zhang, Z., Zhou, Q., Zhou, J., Wakeland, E.K., Fang, X., Xuan, Z., Shen, D., and Li, Q.Z. (2013). Complete genome analysis of three Acinetobacter baumannii clinical isolates in China for insight into the diversification of drug resistance elements. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066584"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1080\/21505594.2021.1878688","article-title":"Pathogenicity and virulence of Staphylococcus aureus","volume":"12","author":"Cheung","year":"2021","journal-title":"Virulence"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1002\/jobm.202100201","article-title":"Antibiotic resistance genes in bacteria: Occurrence, spread, and control","volume":"61","author":"Jian","year":"2021","journal-title":"J. Basic Microb."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zalewska, M., Blazejewska, A., Czapko, A., and Popowska, M. (2021). Antibiotics and Antibiotic Resistance Genes in Animal Manure\u2014Consequences of Its Application in Agriculture. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.610656"}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/12\/12\/916\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:54:57Z","timestamp":1760115297000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/12\/12\/916"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":37,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["toxics12120916"],"URL":"https:\/\/doi.org\/10.3390\/toxics12120916","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,18]]}}}