{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:11:45Z","timestamp":1782803505790,"version":"3.54.5"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Endowed Research Funds","award":["RT082625-BUCH"],"award-info":[{"award-number":["RT082625-BUCH"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Despite the ban of technical chlordane, contamination from this persistent organic pollutant has threatened wildlife and human health nearly forty years since its last application. The purpose of this study is to highlight the need for more systemic, broad-scale research efforts to monitor technical chlordane in wildlife sentinel species in urban settings to understand the nature and extent of pesticide pollution and mitigate risk associated with exposure to these compounds. This study presents an unusual finding of neurotoxicosis and elevated chlordane metabolite concentrations in Michigan striped skunks in the absence of other viral or toxic etiologies. In this study, eight of seventeen skunks displaying illness and neurologic signs had brain tissue concentrations of combined oxychlordane, heptachlor epoxide, and trans-nonachlor exceeding the 1000 ng\/g wet weight diagnostic threshold for toxicosis. Liver tissue concentrations were ten-fold greater than those of the brain when measured on a lipid weight basis, which can help predict lethal brain residues in skunks. The ongoing presence of chlordane in the environment is expected to cause further unintended consequences for wildlife across the Detroit Metropolitan Area for decades to come. Together, veterinary toxicologists, wildlife biologists, environmental toxicologists, ecologists, and policy makers must utilize a One Health transdisciplinary approach and continue to evaluate the long-term effects of chlordane exposure. As with other pollutants in the River Rouge and River Raisin Areas of Concern, the presence of chlordane in the urban environment presents a significant risk for animal, human, and ecological health.<\/jats:p>","DOI":"10.3390\/toxics13050367","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T09:16:12Z","timestamp":1746090972000},"page":"367","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chlordane-Induced Neurotoxicosis in Urban and Suburban Detroit, Michigan Striped Skunks (Mephitis mephitis)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9243-2858","authenticated-orcid":false,"given":"Rachel","family":"Sheffler","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Veterinary Diagnostic Laboratory, Toxicology Section, Michigan State University, Lansing, MI 48910, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6765-5085","authenticated-orcid":false,"given":"Birgit","family":"Puschner","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Veterinary Diagnostic Laboratory, Toxicology Section, Michigan State University, Lansing, MI 48910, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Melotti","sequence":"additional","affiliation":[{"name":"Wildlife Disease Laboratory, Michigan Department of Natural Resources, Lansing, MI 48910, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Fitzgerald","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Veterinary Diagnostic Laboratory, Toxicology Section, Michigan State University, Lansing, MI 48910, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Buchweitz","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Veterinary Diagnostic Laboratory, Toxicology Section, Michigan State University, Lansing, MI 48910, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,1]]},"reference":[{"key":"ref_1","unstructured":"Bosko, T., Crumpacker, A., Kozel, L., Wojdakowski, N., and Lantinga, C. (2010). Data Evaluation Report for Lower Rouge River Sediment Investigation, United States Environmental Protection Agency."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1080\/14634988.2018.1528816","article-title":"The Rouge River Area of Concern\u2014A Multi-Year, Multi-Level Successful Approach to Restoration of Impaired Beneficial Uses","volume":"21","author":"Ridgway","year":"2018","journal-title":"Aquat. Ecosyst. Health Manag."},{"key":"ref_3","unstructured":"Bean, C., Schrameck, R., and Davidson, C. (1994). Rouge River Remedial Action Plan Update, United States Environmental Protection Agency."},{"key":"ref_4","unstructured":"Southeast Michigan Council of Governments, and Michigan Department of Natural Resources (1990). Remedial Action Plan For the River Rouge Basin, United States Environmental Protection Agency."},{"key":"ref_5","unstructured":"McGaughy, R.E., Foureman, G.L., and McClure, P. (1997). Toxicological Review of Chlordane (Technical), United States Environmental Protection Agency."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"692","DOI":"10.2307\/2668517","article-title":"The Stockholm Convention on Persistent Organic Pollutants","volume":"95","author":"Lallas","year":"2001","journal-title":"Am. J. Int. Law"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/BF01685030","article-title":"Persistence and Distribution of Chlordane and Dieldrin Applied as Termiticides","volume":"11","author":"Bennett","year":"1974","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1021\/jf00103a023","article-title":"Metabolism of Cis- and Trans-Chlordane by a Soil Microorganism","volume":"29","author":"Beeman","year":"1981","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"134486","DOI":"10.1016\/j.chemosphere.2022.134486","article-title":"Enantioselective Characteristics, Bioaccumulation and Toxicological Effects of Chlordane-Related Compounds in Laying Hens","volume":"300","author":"Liu","year":"2022","journal-title":"Chemosphere"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/BF01539981","article-title":"Characterization of Oxychlordane, Animal Metabolite of Chlordane","volume":"5","author":"Polen","year":"1970","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1080\/15287398309530453","article-title":"Oxychlordane, Hcs-3260, and Nonachlor in Birds: Lethal Residues and Loss Rates","volume":"12","author":"Stickel","year":"1983","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1021\/jf60180a062","article-title":"Oxychlordane: Accumulation in Rat Adipose Tissue on Feeding Chlordane Isomers or Technical Chlordane","volume":"20","author":"Street","year":"1972","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s002440010174","article-title":"Food Chain Aspects of Chlordane Poisoning in Birds and Bats","volume":"40","author":"Stansley","year":"2001","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/BF01985485","article-title":"Oxychlordane Residues in Soil","volume":"25","author":"Suprock","year":"1980","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chemosphere.2018.02.152","article-title":"DDT Poisoning of Big Brown Bats, Eptesicus Fuscus, in Hamilton, Montana","volume":"201","author":"Buchweitz","year":"2018","journal-title":"Chemosphere"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"84","DOI":"10.2307\/1379877","article-title":"Insecticides Applied to a Nursery Colony of Little Brown Bats (Myotis lucifugus): Lethal Concentrations in Brain Tissues","volume":"59","author":"Clark","year":"1978","journal-title":"J. Mammal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.chemosphere.2006.06.001","article-title":"Persistent Organochlorines in Raccoon Dogs (Nyctereutes procyonoides) from Japan: Hepatic Sequestration of Oxychlordane","volume":"66","author":"Kunisue","year":"2007","journal-title":"Chemosphere"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"591","DOI":"10.2307\/3809354","article-title":"Organochlorine Residues in Mississippi Raccoons","volume":"54","author":"Ford","year":"1990","journal-title":"J. Wildl. Manag."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/BF01622111","article-title":"Oxychlordane, Trans-Nonachlor and Cis-Nonachlor Residues in Adipose Tissues of Dogs and Cats Collected in the Tokyo Area","volume":"26","author":"Yamagishi","year":"1981","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1002\/etc.5620180930","article-title":"Chlordane Poisoning of Birds in New Jersey, USA","volume":"18","author":"Stansley","year":"1999","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111851","DOI":"10.1016\/j.envres.2021.111851","article-title":"Biomonitoring of Toxic Metals, Organochlorine Pesticides, and Polybrominated Biphenyl 153 in Michigan Urban Anglers","volume":"203","author":"Wattigney","year":"2022","journal-title":"Environ. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1080\/15287398109530073","article-title":"Organochlorine Concentrations in Bald Eagles: Brainjbody Lipid Relations and Hazard Evaluation","volume":"8","author":"Barbehenn","year":"1981","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.cvex.2009.01.012","article-title":"Nutrition and Behavior of Striped Skunks","volume":"12","author":"Dragoo","year":"2009","journal-title":"Vet. Clin. N. Am. Exot. Anim. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/00039896.1969.10666814","article-title":"Acute Chlordane Intoxication in a Child","volume":"19","author":"Aldrich","year":"1969","journal-title":"Arch. Environ. Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3109\/15563658308990597","article-title":"Acute Chlordane Intoxication","volume":"20","author":"Olanoff","year":"1983","journal-title":"J. Toxicol. Clin. Toxicol."},{"key":"ref_26","unstructured":"National Toxicology Program (1977). Bioassay of Chlordane for Possible Carcinogenicity, National Cancer Institute."},{"key":"ref_27","first-page":"6","article-title":"Determination of Organochlorine Pesticides in Wildlife Liver and Serum Using Gas Chromatography Tandem Quadrupole Mass Spectrometry","volume":"6","author":"Bokhart","year":"2015","journal-title":"J. Chromatogr. Sep. Tech."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0195-5616(01)00010-9","article-title":"How to Evaluate Toxicant Exposures","volume":"32","author":"Blodgett","year":"2002","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1021\/acs.est.6b05162","article-title":"Statistical Survey of Persistent Organic Pollutants: Risk Estimations to Humans and Wildlife through Consumption of Fish from U.S. Rivers","volume":"51","author":"Batt","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ecoenv.2013.07.028","article-title":"River Otters as Biomonitors for Organochlorine Pesticides, PCBs, and PBDEs in Illinois","volume":"100","author":"Carpenter","year":"2014","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/BF00213301","article-title":"Organochlorine Pesticides and Polychlorinated Biphenyls in the Arctic Ocean Food Web","volume":"22","author":"Hargrave","year":"1992","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/BF01055641","article-title":"Aroclor 1254\u00ae Residues in Birds: Lethal Levels and Loss Rates","volume":"13","author":"Stickel","year":"1984","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"8770","DOI":"10.1007\/s11356-016-6108-4","article-title":"A Comprehensive Approach to Actual Polychlorinated Biphenyls Environmental Contamination","volume":"23","author":"Risso","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_34","unstructured":"Stickel, W.H., Stickel, L.F., and Spann, J.W. (1969). Tissue Residues of Dieldrin in Relation to Mortality in Birds and Mammals. Chemical Fallout: Current Research on Persistent Pesticides. Proceedings of the First Rochester Conference on Toxicity, USGS Publications Warehouse."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF01055640","article-title":"DDE in Birds: Lethal Residues and Loss Rates","volume":"13","author":"Stickel","year":"1984","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/00480169.1960.33397","article-title":"Dieldrin Poisoning of Dogs","volume":"8","author":"Harrison","year":"1960","journal-title":"N. Z. Vet. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0278-6915(02)00229-6","article-title":"Toxicity of the Chlordane Metabolite Oxychlordane in Female Rats: Clinical and Histopathological Changes","volume":"41","author":"Bondy","year":"2003","journal-title":"Food Chem. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/etc.5620210209","article-title":"Cycling of Weathered Chlordane Residues in the Environment: Compositional and Chiral Profiles in Contiguous Soil, Vegetation, and Air Compartments","volume":"21","author":"Mattina","year":"2002","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_39","unstructured":"Chawla, M., Singh, J., and Kaushik, R.D. (2025). Chlordane: Exposure, Biohazard, Current Research, and Precautions. Hazardous Chemicals\u2014Overview, Toxicological Profile, Challenges, and Future Perspectives, Elsevier."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"11","DOI":"10.11648\/j.ajbio.s.2016040301.13","article-title":"Toxic Effects of Organochlorine Pesticides: A Review","volume":"4","author":"Singh","year":"2016","journal-title":"Am. J. BioSci."},{"key":"ref_41","first-page":"11","article-title":"Chiral Chlordane Components in Environmental Matrices","volume":"Volume 1085","author":"Garrison","year":"2011","journal-title":"Chiral Pesticides: Stereoselectivity and Its Consequences"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jglr.2021.12.001","article-title":"Persistent Contaminants of Emerging Concern in a Great Lakes Urban-Dominant Watershed","volume":"48","author":"Baker","year":"2022","journal-title":"J. Great Lakes Res."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/13\/5\/367\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:25:43Z","timestamp":1760030743000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/13\/5\/367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,1]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["toxics13050367"],"URL":"https:\/\/doi.org\/10.3390\/toxics13050367","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,1]]}}}