{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:27:41Z","timestamp":1775194061159,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Hurricanes are increasingly impacting inland water systems, yet their role in mobilizing anthropogenic contaminants remains insufficiently characterized. This study presents a preliminary assessment of organic contaminant loading in the French Broad River, North Carolina, 15 days after flooding from Hurricane Helene. Surface water samples from five sites were analyzed using liquid chromatography\u2013high-resolution mass spectrometry. Targeted analysis quantified 11 per- and polyfluoroalkyl substances (PFASs), with summed concentrations ranging from 1.49 to 70.8 ng\/L. One downstream site exhibited the highest burden, where PFOSs and PFOA exceeded U.S. EPA drinking water maximum contaminant levels. Non-targeted analysis (NTA) and suspect screening identified 468 compounds, with 96 structurally annotated at high confidence. Of these, a large proportion were associated with medium to high hazard potential, particularly for acute aquatic toxicity (69%), developmental toxicity (64%), mutagenic genotoxicity (49%), endocrine disruption (35%), skin irritation (27%), eye irritation (26%), and carcinogenicity (17%). Four EPA priority pollutants\u20144-nitrophenol, 2,4,6-trichlorophenol, pentachlorophenol, and dibutyl phthalate\u2014were also detected. Site-specific chemical profiles suggested inputs from flood-damaged wastewater infrastructure and plastic debris. These findings highlight the susceptibility of freshwater systems to contaminant mobilization during extreme flooding and demonstrate the value of combining NTA and cheminformatics for post-disaster monitoring.<\/jats:p>","DOI":"10.3390\/toxics13110905","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:20:44Z","timestamp":1761196844000},"page":"905","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preliminary Identification of PFAS and Other Emerging Contaminants in the French Broad River, NC Post-Hurricane Helene"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9419-320X","authenticated-orcid":false,"given":"Imari","family":"Walker-Franklin","sequence":"first","affiliation":[{"name":"RTI International, Durham, NC 27713, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Blake","sequence":"additional","affiliation":[{"name":"RTI International, Durham, NC 27713, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"Thorp","sequence":"additional","affiliation":[{"name":"RTI International, Durham, NC 27713, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-7753","authenticated-orcid":false,"given":"Shea","family":"Tuberty","sequence":"additional","affiliation":[{"name":"Department of Biology, Appalachian State University, Boone, NC 28608, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"ref_1","unstructured":"French Broad River Partnership (2025, September 04). About the French Broad River. Available online: https:\/\/frenchbroadriver.org\/about-the-french-broad-river\/."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ansari, R.A., Esimaje, T., Ibrahim, O.M., and Mulrooney, T. (2025). Analysis of Forest Change Detection Induced by Hurricane Helene Using Remote Sensing Data. Forests, 16.","DOI":"10.3390\/f16050788"},{"key":"ref_3","unstructured":"FEMA (2025, September 04). North Carolina Tropical Storm Helene, Available online: https:\/\/www.fema.gov\/disaster\/4827."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hertelendy, A.J., and Ciottone, G.R. (2024). Averting flood-related deaths and injuries from hurricanes: Enhancing hospital resilience. Lancet Reg. Health\u2013Am., 40.","DOI":"10.1016\/j.lana.2024.100930"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s44304-025-00094-3","article-title":"Beyond wind and rainfall: Insights into Hurricane Helene fatalities with the National Risk Index","volume":"2","author":"Meng","year":"2025","journal-title":"NPJ Nat. Hazards"},{"key":"ref_6","unstructured":"Brown, Z. (2025, September 04). Post-Hurricane Helene Landslide Detection and Slope Analysis in Western North Carolina Using Google Earth Engine. Available online: https:\/\/digitalcommons.murraystate.edu\/scholarsweek\/Fall2024\/EES\/2\/."},{"key":"ref_7","unstructured":"Welsh, W., Bassett, H., Fitzgerald, K., Hanson, K., Maher, A., Carroll, C., Averyt, K., Gunter-Bassett, M., Daniels, B., and Wegener, C. (2025, September 04). Rapid Site Report: Potential Impacts to Cultural Heritage Sites from Hurricane Helene, Western North Carolina, USA. Available online: https:\/\/smithsonian.figshare.com\/articles\/report\/Rapid_Site_Report_Potential_Impacts_to_Cultural_Heritage_Sites_from_Hurricane_Helene_Western_North_Carolina_USA\/27558057?file=50360079."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Amorim, R., Villarini, G., Czajkowski, J., and Smith, J. (2025). Flooding from Hurricane Helene and associated impacts: A historical perspective. J. Hydrol. X, 27.","DOI":"10.1016\/j.hydroa.2025.100204"},{"key":"ref_9","unstructured":"USGS (2025, September 04). Hurricane Helene Flooding on French Broad River in Asheville, North Carolina, Available online: https:\/\/www.usgs.gov\/media\/images\/hurricane-helene-flooding-french-broad-river-asheville-north-carolina#:~:text=An%20image%20of%20a%20USGS,USGS%20image."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3775","DOI":"10.1021\/acs.est.0c08814","article-title":"Source-to-Tap Assessment of Microbiological Water Quality in Small Rural Drinking Water Systems in Puerto Rico Six Months After Hurricane Maria","volume":"55","author":"Keenum","year":"2021","journal-title":"Environ. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/s41370-018-0094-1","article-title":"A review of the pathways of human exposure to poly-and perfluoroalkyl substances (PFASs) and present understanding of health effects","volume":"29","author":"Sunderland","year":"2019","journal-title":"J. Expo. Sci. Environ. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ehsan, M.N., Riza, M., Pervez, M.N., Khyum, M.M.O., Liang, Y., and Naddeo, V. (2023). Environmental and health impacts of PFAS: Sources, distribution and sustainable management in North Carolina (USA). Sci. Total Environ., 878.","DOI":"10.1016\/j.scitotenv.2023.163123"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Panieri, E., Baralic, K., Djukic-Cosic, D., Buha Djordjevic, A., and Saso, L. (2022). PFAS molecules: A major concern for the human health and the environment. Toxics, 10.","DOI":"10.3390\/toxics10020044"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9495","DOI":"10.1021\/acs.est.0c01655","article-title":"Impact of Hurricane Maria on Drinking Water Quality in Puerto Rico","volume":"54","author":"Lin","year":"2020","journal-title":"Environ. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.jhydrol.2011.11.010","article-title":"Hurricane Katrina impact on water quality in the East Pearl River, Mississippi","volume":"414","author":"Shiller","year":"2012","journal-title":"J. Hydrol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/BF02798651","article-title":"Effects of hurricane Ivan on water quality in Pensacola Bay, Florida","volume":"29","author":"Hagy","year":"2006","journal-title":"Estuaries Coasts"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1021\/es5002105","article-title":"Identifying small molecules via high resolution mass spectrometry: Communicating confidence","volume":"48","author":"Schymanski","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Hedgespeth, M.L., McCord, J.P., Phillips, K.A., Strynar, M.J., Shea, D., and Nichols, E.G. (2021). Suspect-screening analysis of a coastal watershed before and after Hurricane Florence using high-resolution mass spectrometry. Sci. Total Environ., 782.","DOI":"10.1016\/j.scitotenv.2021.146862"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12135","DOI":"10.1021\/acs.est.4c02934","article-title":"Combining nontargeted analysis with computer-based hazard comparison approaches to support prioritization of unregulated organic contaminants in biosolids","volume":"58","author":"Newmeyer","year":"2024","journal-title":"Environ. Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.coesh.2020.05.005","article-title":"Illuminating the exposome with high-resolution accurate-mass mass spectrometry and nontargeted analysis","volume":"15","author":"Getzinger","year":"2020","journal-title":"Curr. Opin. Environ. Sci. Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.watres.2015.09.017","article-title":"Quantitative target and systematic non-target analysis of polar organic micro-pollutants along the river Rhine using high-resolution mass-spectrometry\u2013identification of unknown sources and compounds","volume":"87","author":"Ruff","year":"2015","journal-title":"Water Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"6237","DOI":"10.1007\/s00216-015-8681-7","article-title":"Non-target screening with high-resolution mass spectrometry: Critical review using a collaborative trial on water analysis","volume":"407","author":"Schymanski","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Han, I., Whitworth, K.W., Christensen, B., Afshar, M., Han, H.A., Rammah, A., Oluwadairo, T., and Symanski, E. (2022). Heavy metal pollution of soils and risk assessment in Houston, Texas following Hurricane Harvey. Environ. Pollut., 296.","DOI":"10.1016\/j.envpol.2021.118717"},{"key":"ref_24","first-page":"53","article-title":"Hurricane Katrina: Environmental hazards in the disaster area","volume":"9","author":"Reible","year":"2007","journal-title":"Cityscape"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Whitaker, J.S., Hrabak, R.B., Ramos, M., Neslund, C., and Li, Y. (2021). An interlaboratory study on EPA methods 537.1 and 533 for per-and polyfluoroalkyl substance analyses. AWWA Water Sci., 3.","DOI":"10.1002\/aws2.1234"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Schulze, B., Jeon, Y., Kaserzon, S., Heffernan, A.L., Dewapriya, P., O\u2019Brien, J., Ramos, M.J.G., Gorji, S.G., Mueller, J.F., and Thomas, K.V. (2020). An assessment of quality assurance\/quality control efforts in high resolution mass spectrometry non-target workflows for analysis of environmental samples. TrAC Trends Anal. Chem., 133.","DOI":"10.1016\/j.trac.2020.116063"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16289","DOI":"10.1021\/acs.analchem.1c02660","article-title":"An introduction to the benchmarking and publications for non-targeted analysis working group","volume":"93","author":"Place","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_28","unstructured":"mzCloud (2025, September 04). Advanced Mass Spectral Database. Available online: https:\/\/www.mzcloud.org\/."},{"key":"ref_29","unstructured":"BP4NTA (2025, September 04). NTA Study Reporting Tool. Available online: https:\/\/figshare.com\/articles\/online_resource\/NTA_Study_Reporting_Tool_Excel_\/19763503?file=47020795."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s10098-019-01795-w","article-title":"An automated framework for compiling and integrating chemical hazard data","volume":"22","author":"Vegosen","year":"2020","journal-title":"Clean Technol. Environ. Policy"},{"key":"ref_31","unstructured":"US Environmental Protection Agency (US EPA) (2011). Design for the Environment Program Alternatives Assessment Criteria for Hazard Evaluation."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1039\/D0EM00291G","article-title":"An overview of the uses of per-and polyfluoroalkyl substances (PFAS)","volume":"22","author":"Scheringer","year":"2020","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3390\/pollutants4010009","article-title":"Overview of Per-and Polyfluoroalkyl Substances (PFAS), Their Applications, Sources, and Potential Impacts on Human Health","volume":"4","author":"Habib","year":"2024","journal-title":"Pollutants"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Martinez, B., Da Silva, B.F., Aristizabal-Henao, J.J., Denslow, N.D., Osborne, T.Z., Morrison, E.S., Bianchi, T.S., and Bowden, J.A. (2022). Increased levels of perfluorooctanesulfonic acid (PFOS) during Hurricane Dorian on the east coast of Florida. Environ. Res., 208.","DOI":"10.1016\/j.envres.2021.112635"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Mi\u0161\u013eanov\u00e1, C., and Valachovi\u010dov\u00e1, M. (2025). Health Impacts of Per- and Polyfluoroalkyl Substances (PFASs): A Comprehensive Review. Life, 15.","DOI":"10.3390\/life15040573"},{"key":"ref_36","unstructured":"North Carolina Department of Environmental Quality Division of Water Resource Water Sciences Section (2025, September 04). Identification of Select Emerging PFAS Compounds in Public Water Supply Reservoirs of the Catawba, French Broad, and Tar River Basins in 2022, Available online: https:\/\/www.deq.nc.gov\/water-resources\/almp2022ecreportfinalcjsignpdf\/open."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6800","DOI":"10.1021\/acs.est.9b05007","article-title":"Trophodynamics of Per- and Polyfluoroalkyl Substances in the Food Web of a Large Atlantic Slope River","volume":"54","author":"Penland","year":"2020","journal-title":"Environ. Sci. Technol."},{"key":"ref_38","unstructured":"ASHEVILLE, Co (2025). PFAS Mitigation Efforts, ASHEVILLE, Co."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s00216-021-03392-7","article-title":"FluoroMatch 2.0-making automated and comprehensive non-targeted PFAS annotation a reality","volume":"414","author":"Koelmel","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1039\/C6GC02744J","article-title":"High-throughput screening of chemicals as functional substitutes using structure-based classification models","volume":"19","author":"Phillips","year":"2017","journal-title":"Green Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1002\/rcm.7263","article-title":"Using the partial least squares method to model the electrospray ionization response produced by small pharmaceutical molecules in positive mode","volume":"29","author":"Mandra","year":"2015","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Williams, A.J., Lambert, J.C., Thayer, K., and Dorne, J.-L.C. (2021). Sourcing data on chemical properties and hazard data from the US-EPA CompTox Chemicals Dashboard: A practical guide for human risk assessment. Environ. Int., 154.","DOI":"10.1016\/j.envint.2021.106566"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/BF00006868","article-title":"Effects of tebuthiuron on aquatic productivity","volume":"224","author":"Temple","year":"1991","journal-title":"Hydrobiologia"},{"key":"ref_44","unstructured":"Townsend, J. (2025, September 04). National Pollutant Discharge Elimination System (NPDES) General Permit for Waste Discharge Requirements (WDRs) for Storm Water Discharges from Small Municipal Separate Storm Sewer Systems (MS4s) Water Quality (WQ) Order 2013-0001-DWQ NPDES NO. CAS000004, Available online: https:\/\/www.epa.gov\/npdes."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Crawford, S.E., Brinkmann, M., Ouellet, J.D., Lehmkuhl, F., Reicherter, K., Schwarzbauer, J., Bellanova, P., Letmathe, P., Blank, L.M., and Weber, R. (2022). Remobilization of pollutants during extreme flood events poses severe risks to human and environmental health. J. Hazard. Mater., 421.","DOI":"10.1016\/j.jhazmat.2021.126691"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12302-023-00717-4","article-title":"Distribution, remobilization and accumulation of organic contaminants by flood events in a meso-scaled catchment system","volume":"35","author":"Schwanen","year":"2023","journal-title":"Environ. Sci. Eur."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.envint.2015.12.008","article-title":"Linking high resolution mass spectrometry data with exposure and toxicity forecasts to advance high-throughput environmental monitoring","volume":"88","author":"Rager","year":"2016","journal-title":"Environ. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"20148","DOI":"10.1021\/acs.est.3c04413","article-title":"Quaternary ammonium compounds (QACs) in wastewater influent and effluent throughout the COVID-19 pandemic","volume":"57","author":"Mahony","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"28352","DOI":"10.1007\/s11356-019-05673-2","article-title":"The impact and mechanism of quaternary ammonium compounds on the transmission of antibiotic resistance genes","volume":"26","author":"Han","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.scitotenv.2015.03.007","article-title":"QUATERNARY ammonium compounds (QACs): A review on occurrence, fate and toxicity in the environment","volume":"518","author":"Zhang","year":"2015","journal-title":"Sci. Total Environ."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1039\/C8EM00588E","article-title":"Exploring co-occurrence patterns between organic micropollutants and bacterial community structure in a mixed-use watershed","volume":"21","author":"Gao","year":"2019","journal-title":"Environ. Sci. Process. Impacts"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Hunter, B., Walker, I., Lassiter, R., Lassiter, V., Gibson, J.M., Ferguson, P.L., and Deshusses, M.A. (2021). Evaluation of private well contaminants in an underserved North Carolina community. Sci. Total Environ., 789.","DOI":"10.1016\/j.scitotenv.2021.147823"},{"key":"ref_53","unstructured":"The United States Geological Survey (USGS) (2025, July 11). National Water Information System Dataset for French Broad River, Available online: https:\/\/waterdata.usgs.gov\/."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.marpolbul.2016.03.044","article-title":"Comparison of wastewater-associated contaminants in the bed sediment of Hempstead Bay, New York, before and after Hurricane Sandy","volume":"107","author":"Fisher","year":"2016","journal-title":"Mar. Pollut. Bull."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/13\/11\/905\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T06:04:36Z","timestamp":1761199476000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/13\/11\/905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,22]]},"references-count":54,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["toxics13110905"],"URL":"https:\/\/doi.org\/10.3390\/toxics13110905","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,22]]}}}