{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:30:02Z","timestamp":1789986602319,"version":"4.0.1"},"reference-count":35,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wuhan Polytechnic University Industry-Academia-Research Project","award":["whpu-2023-kj-2709"],"award-info":[{"award-number":["whpu-2023-kj-2709"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>This study evaluated the skin sensitization of the antimicrobial peptide sublancin to support its safety assessment for topical application. Sensitization was assessed using the guinea pig maximization test (GPMT), in which animals received sublancin (2 mg\/kg), vehicle (negative control), or 2,4-dinitrochlorobenzene (positive control) during induction and challenge phases. Skin reactions (erythema and edema) were recorded after challenge. Irritation was evaluated in rabbits following single and repeated applications of sublancin to intact and abraded skin, with observations made at multiple time points. In the GPMT, no erythema or edema was observed in the sublancin-treated group or negative control group at 24, 48, and 72 h post-challenge, corresponding to a sensitization rate of 0%. All animals in the positive control group exhibited moderate to severe erythema and edema (sensitization rate 100%). In both single- and repeated-dose rabbit irritation tests, sublancin induced no erythema or edema on intact or abraded skin at any observation point, resulting in a total irritation score of 0. Furthermore, no significant differences in the daily weight gain were observed between any experimental group and the negative group (p &gt; 0.05). In conclusion, under the conditions of this study, sublancin showed no skin sensitization potential in guinea pigs and no irritant effects in rabbits, supporting its local tolerance for topical veterinary use.<\/jats:p>","DOI":"10.3390\/toxics14010069","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T10:25:23Z","timestamp":1768213523000},"page":"69","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antimicrobial Peptide Sublancin Skin Sensitization and Irritation Assessment in Guinea Pigs and Rabbits"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4779-0023","authenticated-orcid":false,"given":"Yong","family":"Guo","sequence":"first","affiliation":[{"name":"School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 420200, China"},{"name":"Sinagri YingTai Bio-peptide Co., Ltd., Linzhou 456550, China"},{"name":"Key Laboratory of Feed Antibiotics Replacement Technology, Ministry of Agriculture and Rural Affairs, Linzhou 456550, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gantong","family":"Guo","sequence":"additional","affiliation":[{"name":"Sinagri YingTai Bio-peptide Co., Ltd., Linzhou 456550, China"},{"name":"Key Laboratory of Feed Antibiotics Replacement Technology, Ministry of Agriculture and Rural Affairs, Linzhou 456550, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"He","sequence":"additional","affiliation":[{"name":"Sinagri YingTai Bio-peptide Co., Ltd., Linzhou 456550, China"},{"name":"Key Laboratory of Feed Antibiotics Replacement Technology, Ministry of Agriculture and Rural Affairs, Linzhou 456550, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangke","family":"Liu","sequence":"additional","affiliation":[{"name":"Sinagri YingTai Bio-peptide Co., Ltd., Linzhou 456550, China"},{"name":"Key Laboratory of Feed Antibiotics Replacement Technology, Ministry of Agriculture and Rural Affairs, Linzhou 456550, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0982-1143","authenticated-orcid":false,"given":"Yujiao","family":"Lai","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"179867","DOI":"10.1016\/j.scitotenv.2025.179867","article-title":"Antibiotic alternatives in livestock feeding","volume":"989","author":"Liu","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"112464","DOI":"10.1016\/j.ecoenv.2021.112464","article-title":"Sub-chronic exposure to antibiotics doxycycline, oxytetracycline or florfenicol impacts gut barrier and induces gut microbiota dysbiosis in adult zebrafish (Daino rerio)","volume":"221","author":"Qian","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1007\/s10495-024-01937-6","article-title":"Induction of the mitochondrial pathway of apoptosis by enrofloxacin in the context of the safety issue of its use in poultry","volume":"29","author":"Grabowski","year":"2024","journal-title":"Apoptosis"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1007\/s11427-022-2246-4","article-title":"A comprehensive review on natural phenolic compounds as alternatives to in-feed antibiotics","volume":"66","author":"Gao","year":"2023","journal-title":"Sci. China Life Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/s41579-025-01200-y","article-title":"Antimicrobial peptides: Structure, functions and translational applications","volume":"23","author":"Souza","year":"2025","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"43918","DOI":"10.1021\/acsami.4c06381","article-title":"Versatile Fabrication of Biocompatible Antimicrobial Materials Enabled by Cationic Peptide Bundles","volume":"16","author":"Liu","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"23134","DOI":"10.1074\/jbc.273.36.23134","article-title":"Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168","volume":"273","author":"Paik","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4750","DOI":"10.2527\/jas.2015-9284","article-title":"The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by Clostridium perfringens in broilers","volume":"93","author":"Wang","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8595","DOI":"10.1021\/acs.jafc.7b02592","article-title":"Use of the Antimicrobial Peptide Sublancin with Combined Antibacterial and Immunomodulatory Activities To Protect against Methicillin-Resistant Staphylococcus aureus Infection in Mice","volume":"65","author":"Wang","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"170533","DOI":"10.1016\/j.peptides.2021.170533","article-title":"Sublancin protects against methicillin-resistant Staphylococcus aureus infection by the combined modulation of innate immune response and microbiota","volume":"141","author":"Li","year":"2021","journal-title":"Peptides"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1002\/ar.22941","article-title":"The Bacteriocin Sublancin Attenuates Intestinal Injury in Young Mice Infected With Staphylococcus aureus","volume":"297","author":"Wang","year":"2014","journal-title":"Anat. Rec."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Guo, Y., Li, Z., Xu, P., Guo, G., He, T., and Lai, Y. (2025). Subchronic and Chronic Toxicity Assessment of Sublancin in Sprague-Dawley Rats. Toxics, 13.","DOI":"10.3390\/toxics13050413"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1021\/acsbiomaterials.3c01551","article-title":"Porous NiTi Dental Implant Fabricated by a Metal Injection Molding: An in Vivo Biocompatibility Evaluation in an Animal Model","volume":"10","author":"Mustafa","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e12142","DOI":"10.1002\/1348-9585.12142","article-title":"Trichloroethylene and trichloroethanol induce skin sensitization with focal hepatic necrosis in guinea pigs","volume":"62","author":"Zhao","year":"2020","journal-title":"J. Occup. Health"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1021\/cb4008106","article-title":"NMR structure of the S-linked glycopeptide sublancin 168","volume":"9","author":"Zhu","year":"2014","journal-title":"ACS Chem. Biol."},{"key":"ref_16","unstructured":"OECD (2015). Test No. 404: Acute Dermal Irritation\/Corrosion. OECD Guidelines for the Testing of Chemicals, OECD Publishing. Section 4."},{"key":"ref_17","unstructured":"OECD (1992). Test No. 406: Skin Sensitisation. OECD Guidelines for the Testing of 1203 Chemicals, OECD Publishing. Section 4: Health Effects."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"142930","DOI":"10.1016\/j.chemosphere.2024.142930","article-title":"Health risks from exposure to chemicals in clothing\u2014Non-regulated halogenated aromatic compounds","volume":"363","author":"Carlsson","year":"2024","journal-title":"Chemosphere"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1016\/j.jid.2024.01.022","article-title":"Costimulatory Molecule CD226 Regulates Atopic Dermatitis in a Mouse Model","volume":"144","author":"Qiao","year":"2024","journal-title":"J. Investig. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12934-015-0201-0","article-title":"Improved production of sublancin via introduction of three characteristic promoters into operon clusters responsible for this novel distinct glycopeptide biosynthesis","volume":"14","author":"Ji","year":"2015","journal-title":"Microb. Cell Fact."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s13346-021-00898-6","article-title":"Influencing factors and drug application of iontophoresis in transdermal drug delivery: An overview of recent progress","volume":"12","author":"Wang","year":"2022","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"115316","DOI":"10.1016\/j.biopha.2023.115316","article-title":"Cubosomes-assisted transdermal delivery of doxorubicin and indocyanine green for chemo-photothermal combination therapy of melanoma","volume":"166","author":"Chen","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"114208","DOI":"10.1016\/j.celrep.2024.114208","article-title":"Rho-associated kinase regulates Langerhans cell morphology and responsiveness to tissue damage","volume":"43","author":"Peterman","year":"2024","journal-title":"Cell Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1007\/s00204-025-04013-3","article-title":"Development of image analysis tool to evaluate Langerhans cell migration after exposure to isothiazolinones","volume":"99","author":"Oltramare","year":"2025","journal-title":"Arch. Toxicol."},{"key":"ref_25","unstructured":"UNECE (United Nations Economic Commission for Europe) (2026, January 07). GHS (Rev.8) (2019): Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Part 3.3. Available online: https:\/\/unece.org\/ghs-rev8-2019."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1751-0813.2009.00546.x","article-title":"Topical anaesthesia alleviates short-term pain of castration and tail docking in lambs","volume":"88","author":"Lomax","year":"2010","journal-title":"Aust. Vet. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/s41538-025-00519-3","article-title":"Portable and affordable device for on-site quantitative detection of somatic cells in milk","volume":"9","author":"Tammineni","year":"2025","journal-title":"NPJ Sci. Food"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1186\/s40168-024-01980-0","article-title":"Shotgun metagenomic sequencing reveals the influence of artisanal dairy environments on the microbiomes, quality, and safety of Idiazabal, a raw ewe milk PDO cheese","volume":"12","author":"Yap","year":"2024","journal-title":"Microbiome"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101952","DOI":"10.1016\/j.redox.2021.101952","article-title":"Cutaneous antimicrobial peptides: New \u201cactors\u201d in pollution related inflammatory conditions","volume":"41","author":"Woodby","year":"2021","journal-title":"Redox Biol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"de Alcantara Sica de Toledo, L., Rosseto, H.C., Buccini, D.F., and Franco, O.L. (2025). Synergy between Antimicrobial Peptides and Lipid Nanoparticles for Skin Infection Control. ACS Appl. Mater. Interfaces.","DOI":"10.1021\/acsami.5c18033"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1097\/SLA.0000000000005508","article-title":"Efficacy and Safety of PL-5 (Peceleganan) Spray for Wound Infections: A Phase IIb Randomized Clinical Trial","volume":"277","author":"Wei","year":"2023","journal-title":"Ann. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1021\/acschembio.7b00819","article-title":"Structure-Activity Relationships of the S-Linked Glycocin Sublancin","volume":"12","author":"Biswas","year":"2017","journal-title":"ACS Chem. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3979352","DOI":"10.1155\/2019\/3979352","article-title":"Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus","volume":"2019","author":"Wang","year":"2019","journal-title":"J. Immunol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e2309568","DOI":"10.1002\/smll.202309568","article-title":"A Photo-induced Cross-Linking Enhanced A and B Combined Multi-Functional Spray Hydrogel Instantly Protects and Promotes of Irregular Dynamic Wound Healing","volume":"20","author":"Zhong","year":"2024","journal-title":"Small"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"114190","DOI":"10.1016\/j.jconrel.2025.114190","article-title":"Raman spectroscopy as a tool to accelerate development of complex medicinal products","volume":"387","author":"Zarmpi","year":"2025","journal-title":"J. Control. 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