{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:50:25Z","timestamp":1768956625873,"version":"3.49.0"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ningxia Medical University Scientific Research Project","award":["XZ2024002"],"award-info":[{"award-number":["XZ2024002"]}]},{"name":"Ningxia Medical University Scientific Research Project","award":["FP20230103"],"award-info":[{"award-number":["FP20230103"]}]},{"DOI":"10.13039\/501100004772","name":"Ningxia Natural Science Foundation","doi-asserted-by":"publisher","award":["2022AAC05027"],"award-info":[{"award-number":["2022AAC05027"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ningxia Medical University High-level Talent Project","award":["XT2023028"],"award-info":[{"award-number":["XT2023028"]}]},{"name":"Director Fund of the Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University","award":["FP20240102"],"award-info":[{"award-number":["FP20240102"]}]},{"name":"Ningxia Medical University Interdisciplinary Disciplines","award":["JCXK2025002"],"award-info":[{"award-number":["JCXK2025002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The long-term inhalation of free silica dust causes silicosis\u2014a prevalent occupational hazard\u2014yet its systemic effect on male reproductive toxicity remains underexplored. Tetrandrine (Tet) is the only plant-derived anti-silicosis drug approved in China. This study investigates silica (SiO2) -induced male reproductive damage and evaluates Tet\u2019s protective effects. Sixty male C57BL\/6 mice (6\u20138 weeks) were divided into control, SiO2 exposure, and SiO2 + Tet groups. SiO2 was administered via intranasal infusion and Tet via gavage. Mice were sacrificed at day 7 (male reproductive injury model corresponding to the pulmonary inflammation stage) and day 42 (male reproductive injury model corresponding to the pulmonary fibrosis stage). Analyses included sperm morphology, testicular transcriptome sequencing, RT-qPCR, and immunofluorescence. At day 7, SiO2 exposure upregulated testicular inflammatory markers, which were partially mitigated by Tet. At day 42, SiO2 increased sperm deformity and testicular fibrosis markers (fibronectin and vimentin); Tet intervention reduced these abnormalities. Transcriptome analysis revealed distinct gene expression patterns at day 7 versus day 42, indicating time-dependent injury mechanisms. Tetrandrine alleviates silica-induced reproductive damage in male mice, suggesting potential therapeutic applications for occupational silica exposure and expanding the understanding of silica toxicity beyond the respiratory system.<\/jats:p>","DOI":"10.3390\/toxics14010087","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:24:34Z","timestamp":1768901074000},"page":"87","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Protective Effect and Molecular Mechanism of Tetrandrine on Male Reproductive Damage Caused by Silicon Dioxide"],"prefix":"10.3390","volume":"14","author":[{"given":"Hong-Mei","family":"Li","sequence":"first","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Qi","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Chang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Xin","family":"Sheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Jia","family":"Pu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Cheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Hui","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Xuan","sequence":"additional","affiliation":[{"name":"The Fifth People\u2019s Hospital of the Ningxia Hui Autonomous Region, Shizuishan 753000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-000X","authenticated-orcid":false,"given":"Hai-Ming","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, China"},{"name":"The Key Laboratory of Environmental Factors and Chronic Disease Control, School of Public Health, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Poinen-Rughooputh, S., Rughooputh, M.S., Guo, Y., Rong, Y., and Chen, W. 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