{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:24:51Z","timestamp":1770978291493,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872584"],"award-info":[{"award-number":["81872584"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Shenzhen","award":["JCYJ20210324093211030"],"award-info":[{"award-number":["JCYJ20210324093211030"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZSM202211009"],"award-info":[{"award-number":["SZSM202211009"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Henan Province\u2019s Key R&D and Promotion Projects (Scientific and Technological Research) Projects","award":["252102310075"],"award-info":[{"award-number":["252102310075"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Microplastics (MPs) are pervasive contaminants that enter the food chain and cause health issues. However, the size-dependent effects of MPs on lipid metabolism remain inadequately characterized. Using Caenorhabditis elegans (C. elegans), we investigated the size-dependent toxicity of polystyrene (PS)-MPs as model contaminants with sizes of 100 nm and 1 \u03bcm, respectively. We evaluated multiple phenotypic endpoints, including lifespan, growth (body length and width), locomotion (head thrashes and body bends), reproduction, and intestinal lipofuscin. The expression of representative lipid metabolism-related transcripts was validated by quantitative PCR. Untargeted metabolomics profiling detected 831 differential metabolites (451down-regulated and 380 up-regulated) across both PS particle exposure groups, with over-representation of lipid metabolic pathways. Integration of multi-omics (transcriptomics and metabolomics) highlighted acdh-1, ech-6, hach-1, and sur-5 as core lipid-metabolism genes; RNA interference confirmed that knockdown of these target genes abolished the size-dependent differences in fat accumulation induced by MPs. Notably, it revealed elevated linoleic acid and taurocholic acid, signature metabolites indicative of disrupted lipid turnover by our metabolomic profiling. Collectively, our findings demonstrate that exposure to PS-MPs disrupts lipid homeostasis in C. elegans by perturbing mitochondrial function and key metabolic pathways, which in turn impairs growth, development, feeding, and reproductive capacity. Critically, these disruptive effects exhibit a strong size dependency, with 100 nm PS particles inducing more severe perturbations than the 1 \u03bcm particles, and provide novel mechanistic insight into MP-induced metabolic abnormalities, underscoring the importance of considering particle size in assessing the environmental and health risks of MP contamination.<\/jats:p>","DOI":"10.3390\/toxics14020170","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:09:19Z","timestamp":1770973759000},"page":"170","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Size-Dependent Disruption of Lipid Metabolism by Polystyrene Micro- and Nanoplastics in Caenorhabditis elegans Revealed Through Multi-Omics and Functional Genetic Validation"],"prefix":"10.3390","volume":"14","author":[{"given":"Zhi","family":"Qu","sequence":"first","affiliation":[{"name":"Institute of Environment and Health, South China Hospital, Medical School, Shenzhen University, Shenzhen 518116, China"},{"name":"School of Nursing and Health, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xihua","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Environment and Health, South China Hospital, Medical School, Shenzhen University, Shenzhen 518116, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yalu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Nursing and Health, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Nursing and Health, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8895-3169","authenticated-orcid":false,"given":"Nan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Environment and Health, South China Hospital, Medical School, Shenzhen University, Shenzhen 518116, China"},{"name":"School of Nursing and Health, Henan University, Kaifeng 475004, China"},{"name":"College of Public Health, Zhengzhou University, Zhengzhou 540001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127330","DOI":"10.1016\/j.jhazmat.2021.127330","article-title":"Forecasting plastic waste generation and interventions for environmental hazard mitigation","volume":"424","author":"Fan","year":"2022","journal-title":"J. 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