{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:35:53Z","timestamp":1773282953448,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2025 Guizhou Science and Technology Support Plan Project","award":["095"],"award-info":[{"award-number":["095"]}]},{"name":"Foundation of Guizhou Science Cooperation","award":["490"],"award-info":[{"award-number":["490"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>As emerging pollutants, nanoplastics (NPs) are profoundly threatening aquatic ecosystems. However, the systematic response mechanisms of aquatic floating macrophytes to NP stress and the mitigation strategies of nanoselenium (Se) remain poorly understood. This study used P. stratiotes, a dominant species in freshwater ecological restoration, as the research object. By intervening in NP stress via foliar application of Se, the study systematically deciphered the plant\u2019s response and mitigation mechanisms to NPs pollution through integrating physiological and biochemical analyses, ultrastructural observation of cells, and transcriptomic and metabolomic multi-omics techniques. The results showed that NP stress significantly reduced photosynthetic pigment concentration and inhibited photosystem function in Pistia stratiotes L., disrupted energy metabolism homeostasis, and simultaneously induced an outburst of reactive oxygen species (ROS). It activated non-enzymatic antioxidant substances such as flavonoids and glutathione (GSH), as well as enzymatic defense systems including catalase (CAT) and peroxidase (POD), promoting the reprogramming of the plant\u2019s metabolic strategy from growth priority to defense dominance. At the transcriptomic level, NP stress significantly altered the gene expression profile, with core pathways enriched in photosynthesis antenna proteins and phenylpropanoid biosynthesis. Metabolomic analysis revealed significant differences in metabolites, with markedly upregulated contents of defense-related metabolites such as lipids and terpenoids. The intervention of NPs-Se effectively restored photosynthetic pigment contents and enzyme activities, alleviated cell membrane damage by repairing the photosynthetic apparatus, optimizing ribosome-mediated protein synthesis pathways, and strengthening the antioxidant defense network. Meanwhile, it regulated the expression of specific genes and the accumulation of core differential metabolites, reconstructed the balance between energy supply and defense investment, enabling the plant to achieve more efficient adaptive regulation. Multi-omics correlation analysis further confirmed that the responses of P. stratiotes to NPs and NPs-Se exhibited characteristics of coordinated regulation, highlighting the modular regulatory patterns of nano-stress responses. In conclusion, Se can effectively alleviate the stress damage of nanoplastics to P. stratiotes through multi-dimensional regulation, providing a key experimental basis and theoretical support for the ecological restoration of NP-polluted water bodies and ecological risk assessment.<\/jats:p>","DOI":"10.3390\/toxics14030244","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T10:43:27Z","timestamp":1773225807000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Revealing the Selenium-Mediated Regulatory Mechanisms of P. stratiotes in Response to Nanoplastics Stress from Multiple Perspectives of Transcriptomics, Metabolomics, and Plant Physiology"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9946-6705","authenticated-orcid":false,"given":"Sixi","family":"Zhu","sequence":"first","affiliation":[{"name":"The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, College of Eco-Environment Engineering, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Ban","sequence":"additional","affiliation":[{"name":"The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, College of Eco-Environment Engineering, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haobin","family":"Yang","sequence":"additional","affiliation":[{"name":"The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, College of Eco-Environment Engineering, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, College of Eco-Environment Engineering, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhui","family":"Lu","sequence":"additional","affiliation":[{"name":"The Karst Environmental Geological Hazard Prevention of Key Laboratory of State Ethnic Affairs Commission, College of Eco-Environment Engineering, Guizhou Minzu University, Guiyang 550025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e1700782","DOI":"10.1126\/sciadv.1700782","article-title":"Production, use, and fate of all plastics ever made","volume":"3","author":"Geyer","year":"2017","journal-title":"Sci. Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1126\/science.abb5979","article-title":"Microplastic in terrestrial ecosystems","volume":"368","author":"Rillig","year":"2020","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.watres.2019.04.060","article-title":"Municipal solid waste (MSW) landfill: A source of microplastics\u2014Evidence of microplastics in landfill leachate","volume":"159","author":"He","year":"2019","journal-title":"Water Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/s41565-020-0707-4","article-title":"Differentially charged nanoplastics demonstrate distinct accumulation in Arabidopsis thaliana","volume":"15","author":"Sun","year":"2020","journal-title":"Nat. Nanotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.watres.2016.01.002","article-title":"Transport and fate of microplastic particles in wastewater treatment plants","volume":"91","author":"Carr","year":"2016","journal-title":"Water Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"131151","DOI":"10.1016\/j.chemosphere.2021.131151","article-title":"Microplastics in aquatic environment: Challenges and perspectives","volume":"282","author":"Vivekanand","year":"2021","journal-title":"Chemosphere"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"127241","DOI":"10.1016\/j.jhazmat.2021.127241","article-title":"Nanotoxicological effects and transcriptome mechanisms of wheat (Triticum aestivum L.) under stress of polystyrene nanoplastics","volume":"423","author":"Lian","year":"2022","journal-title":"J. Hazard Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"108201","DOI":"10.1016\/j.plaphy.2023.108201","article-title":"Combination of transcriptomics, metabolomics, and physiological traits reveals the effects of polystyrene microplastics on photosynthesis, carbon, and nitrogen metabolism in cucumber (Cucumis sativus L.)","volume":"205","author":"Zhuang","year":"2023","journal-title":"Plant Physiol. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"123412","DOI":"10.1016\/j.jhazmat.2020.123412","article-title":"Response of rice (Oryza sativa L.) roots to nanoplastic treatment at the seedling stage","volume":"401","author":"Zhou","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1002\/jat.4598","article-title":"Genotoxic and neurotoxic potential of intracellular nanoplastics: A review","volume":"44","author":"Casella","year":"2024","journal-title":"Appl. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1021\/acschembio.6b00031","article-title":"Why nature chose selenium","volume":"11","author":"Reich","year":"2016","journal-title":"ACS Chem. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6974","DOI":"10.1038\/s41467-022-34731-6","article-title":"Understanding soil selenium accumulation and bioavailability through size resolved and elemental characterization of soil extracts","volume":"13","author":"Tolu","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"153712","DOI":"10.1016\/j.scitotenv.2022.153712","article-title":"Multiomics understanding of improved quality in cherry radish (Raphanus sativus L. var. radculus pers) after foliar application of selenium nanomaterials","volume":"824","author":"Cheng","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6789","DOI":"10.1021\/acs.jafc.5c02034","article-title":"Selenium nanoparticles vs selenite fertilizers: Implications for toxicological profiles, antioxidant defense, and ferroptosis pathways","volume":"73","author":"Fang","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","first-page":"7845","article-title":"Selenium-based nanomaterials alleviate nanoplastic-induced physiological disorders in rice by remodeling defense signaling and strengthening cellular barriers","volume":"59","author":"Jin","year":"2025","journal-title":"Environ. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1093\/nar\/gkac1069","article-title":"FERONIA coordinates plant growth and salt tolerance via the phosphorylation of phyB","volume":"9","author":"Zhao","year":"2023","journal-title":"Nat. Plants"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhu, S.X., Sun, S.X., Zhao, W., Yang, X.Q., Mao, H., Sheng, L.Y., and Chen, Z.B. (2024). Utilizing transcriptomics and proteomics to unravel key genes and proteins of Oryza sativa seedlings mediated by selenium in response to cadmium stress. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-05076-7"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhao, W., Chen, X., Wang, J., Cheng, Z., Ma, X., Zheng, Q., Xu, Z., and Zhang, F. (2025). Emerging mechanisms of plant responses to abiotic stress. Plants, 14.","DOI":"10.3390\/plants14223445"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/ng.3305","article-title":"Natural alleles of a proteasome \u03b12 subunit gene contribute to thermotolerance and adaptation of African rice","volume":"47","author":"Li","year":"2015","journal-title":"Nat. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"139349","DOI":"10.1016\/j.foodchem.2024.139349","article-title":"Selenium alleviates the adverse effects of microplastics on kale by regulating photosynthesis, redox homeostasis, secondary metabolism and hormones","volume":"450","author":"Tong","year":"2024","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"He, W.Z., Rong, T., Liu, X.Y., and Rao, Q. (2024). Transcriptomic Profiling Unravels the Disruption of Photosynthesis Apparatuses and Induction of Immune Responses by a Bipartite Begomovirus in Tomato Plants. Plants, 13.","DOI":"10.3390\/plants13223198"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"108210","DOI":"10.1016\/j.plaphy.2023.108210","article-title":"Auxin homeostasis in plant responses to heavy metal stress","volume":"205","author":"Yang","year":"2023","journal-title":"Plant Physiol. Biochem. PPB"},{"key":"ref_23","first-page":"162107","article-title":"Effect and mechanism of microplastics exposure against microalgae: Photosynthesis and oxidative stress","volume":"872","author":"Li","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Zhong, L., Xiao, W., Du, Y., Han, G., Yan, Z., He, D., and Zheng, C. (2023). Transcriptomics combined with physiological analysis reveals the mechanism of cadmium uptake and tolerance in Ligusticum chuanxiong Hort. under cadmium treatment. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1263981"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1105\/tpc.107.057380","article-title":"Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression","volume":"20","author":"Qin","year":"2008","journal-title":"Plant Cell"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Peremyslov, V.V., Cole, R.A., Fowler, J.E., and Dolja, V.V. (2015). Myosin-powered membrane compartment drives cytoplasmic streaming, cell expansion and plant development. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139331"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1007\/s11356-020-10706-2","article-title":"Nitric oxide and selenium nanoparticles confer changes in growth, metabolism, antioxidant machinery, gene expression, and flowering in chicory (Cichorium intybus L.): Potential benefits and risk assessment","volume":"28","author":"Abedi","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"124923","DOI":"10.1016\/j.ijbiomac.2023.124923","article-title":"Seedling nanopriming with selenium-chitosan nanoparticles mitigates the adverse effects of salt stress by inducing multiple defense pathways in bitter melon plants","volume":"242","author":"Sheikhalipour","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","first-page":"1479","article-title":"Arabidopsis PUB9, a U-box E3 ubiquitin ligase, is involved in ABA signaling and drought stress responses","volume":"49","author":"Cho","year":"2008","journal-title":"Plant Cell Physiol."},{"key":"ref_30","first-page":"123","article-title":"Selenium nanoparticles reprogram phenylpropanoid metabolic flux to enhance plant defense and stress tolerance","volume":"22","author":"Liu","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Li, D., Zhou, C., Ma, J., Wu, Y., Kang, L., An, Q., Zhang, J., Deng, K., Li, J.Q., and Pan, C. (2021). Nanoselenium transformation and inhibition of cadmium accumulation by regulating the lignin biosynthetic pathway and plant hormone signal transduction in pepper plants. BMC Plant Biol., 21.","DOI":"10.21203\/rs.3.rs-824087\/v1"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"38215","DOI":"10.1038\/srep38215","article-title":"Transcriptome analysis confers a complex disease resistance network in wild rice Oryza meyeriana against Xanthomonas oryzae pv. oryzae","volume":"6","author":"Cheng","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.pbi.2008.05.004","article-title":"Jasmonate signaling: A conserved mechanism of hormone sensing","volume":"11","author":"Katsir","year":"2008","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_34","first-page":"117452","article-title":"Selenium nanoparticles (NPs-Se) mitigate abiotic stress by modulating starch and sucrose metabolism and carbohydrate allocation in aquatic plants","volume":"333","author":"Gupta","year":"2023","journal-title":"J. Environ. Manag."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wang, X., Kong, L., Zhi, P., and Chang, C. (2020). Update on Cuticular Wax Biosynthesis and Its Roles in Plant Disease Resistance. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155514"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1105\/tpc.18.00653","article-title":"The E3 ubiquitin ligase HAF1 modulates circadian accumulation of EARLY FLOWERING3 to control heading date in rice under long-day conditions","volume":"30","author":"Zhu","year":"2018","journal-title":"Plant Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.plantsci.2007.06.001","article-title":"Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis","volume":"173","author":"Park","year":"2007","journal-title":"Plant Sci."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/14\/3\/244\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T11:27:58Z","timestamp":1773228478000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/14\/3\/244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,11]]},"references-count":37,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["toxics14030244"],"URL":"https:\/\/doi.org\/10.3390\/toxics14030244","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,11]]}}}