{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:24:05Z","timestamp":1787750645690,"version":"build-2784847793"},"reference-count":46,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFC1807700"],"award-info":[{"award-number":["2020YFC1807700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Mining and smelting activities have accelerated the release of antimony (Sb) and arsenic (As) into the environment, resulting in high ecological risks. Microorganisms are considered key drivers of Sb and As cycling. However, their effects on Sb\/As transformation in antimony smelting soils remain poorly understood. In this study, an indigenous microbial consortium capable of oxidizing both Sb and As was obtained from antimony smelting soil and used to investigate the interactions between microorganisms and Sb-As co-contaminated soils. The results showed that the functional microbial consortium enhanced Sb and As release from the soil by increasing the system pH from 7.41 to 8.89, thereby promoting the dissolution of solid-phase Sb and As. The concentrations of total dissolved Sb and As reached 246.47 and 30.5 \u00b5mol L\u22121 by day 16, respectively, and the released Sb(III) and As(III) were completely oxidized to Sb(V) and As(V). In the abiotic control, the corresponding concentrations of total dissolved Sb and As were 95.89 and 12.08 \u00b5mol L\u22121, respectively, with an As(III) concentration of 3.54 \u00b5mol L\u22121. Sequential extraction results revealed that microbial activity altered the speciation distribution of Sb and As in soils, decreasing the proportions of easily exchangeable and specifically surface-bound fractions while increasing the residual fractions. X-ray photoelectron spectroscopy (XPS) analysis further showed that, compared with the original soil, the functional microbial consortium increased the proportions of Sb(V) and As(V) in the solid phase from 34.79% to 40.54% and from 9.53% to 15.96%, respectively, suggesting that microbial activity might promote the precipitation of Sb(V) and As(V), thereby facilitating the re-immobilization of a fraction of the released Sb and As. Furthermore, the succession of microbial communities during the interaction process was investigated. The release of Sb and As from soil increased the abundance of microorganisms related to Sb\/As metabolism. Spearman correlation analysis suggested that Arenimonas, Luteimonas, Arthrobacter, and Brevundimonas were likely involved in Sb(III)\/As(III) oxidation, whereas Devosia and Aminobacter contributed to the elevation of system pH. This study provides insights into the microbial-mediated transformation, migration, and oxidation of Sb and As in soils.<\/jats:p>","DOI":"10.3390\/toxics14090759","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:59:57Z","timestamp":1787749197000},"page":"759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Release and Transformation of Sb and As Induced by Microorganisms in Antimony Smelting Soil"],"prefix":"10.3390","volume":"14","author":[{"given":"Yan","family":"Zheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China"},{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China"},{"name":"College of Water Sciences, Beijing Normal University, Beijing 100875, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China"},{"name":"School of Ocean Food and Biological Engineering, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6109-3465","authenticated-orcid":false,"given":"Hong","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jes.2018.05.023","article-title":"Antimony speciation in the environment: Recent advances in understanding the biogeochemical processes and ecological effects","volume":"75","author":"He","year":"2019","journal-title":"J. Environ. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"130100","DOI":"10.1016\/j.jhazmat.2022.130100","article-title":"Soil particle size fractions affect arsenic (As) release and speciation: Insights into dissolved organic matter and functional genes","volume":"443","author":"Zou","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1080\/15275922.2021.1907816","article-title":"Antimony and arsenic contamination in water from antimonite mineralization: A case study from Turhal (Tokat, Northern Turkey)","volume":"23","author":"Kurt","year":"2022","journal-title":"Environ. Forensics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"106834","DOI":"10.1016\/j.gexplo.2021.106834","article-title":"Metallogenic characteristics and resource potential of antimony in China","volume":"230","author":"Ding","year":"2021","journal-title":"J. Geochem. Explor."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"124989","DOI":"10.1016\/j.jclepro.2020.124989","article-title":"Current knowledge from heavy metal pollution in Chinese smelter contaminated soils, health risk implications and associated remediation progress in recent decades: A critical review","volume":"286","author":"Xu","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.scitotenv.2011.06.009","article-title":"Antimony pollution in China","volume":"421","author":"He","year":"2012","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"107897","DOI":"10.1016\/j.envint.2023.107897","article-title":"Soil microbial responses to simultaneous contamination of antimony and arsenic in the surrounding area of an abandoned antimony smelter in Southwest China","volume":"174","author":"Gong","year":"2023","journal-title":"Environ. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"179249","DOI":"10.1016\/j.scitotenv.2025.179249","article-title":"Collaborative effects of antimony-arsenic contaminations on microbial communities in the typical antimony mining areas of Southwest China","volume":"975","author":"Zhao","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7326","DOI":"10.1021\/acs.est.7b00689","article-title":"Linking Genes to Microbial Biogeochemical Cycling: Lessons from Arsenic","volume":"51","author":"Zhu","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5482","DOI":"10.1128\/AEM.01375-16","article-title":"Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways","volume":"82","author":"Li","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"136451","DOI":"10.1016\/j.scitotenv.2019.136451","article-title":"Impacts of antimony and arsenic co-contamination on the river sedimentary microbial community in an antimony-contaminated river","volume":"713","author":"Xu","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"136250","DOI":"10.1016\/j.jhazmat.2024.136250","article-title":"Alteration of soil microbiomes in an arsenic and antimony co-contamination zone after dam failure","volume":"480","author":"Tian","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ecoenv.2014.10.009","article-title":"Arsenic contamination, consequences and remediation techniques: A review","volume":"112","author":"Singh","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107221","DOI":"10.1016\/j.envint.2022.107221","article-title":"Arsenic bioaccumulation and biotransformation in aquatic organisms","volume":"163","author":"Zhang","year":"2022","journal-title":"Environ. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4304","DOI":"10.1016\/S0043-1354(00)00182-2","article-title":"Arsenic: Occurrence, toxicity and speciation techniques","volume":"34","author":"Jain","year":"2000","journal-title":"Water Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Deng, R., Chen, Y., Deng, X., Huang, Z., Zhou, S., Ren, B., Jin, G., and Hursthouse, A. (2021). A Critical Review of Resistance and Oxidation Mechanisms of Sb-Oxidizing Bacteria for the Bioremediation of Sb(III) Pollution. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.738596"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s11157-017-9448-8","article-title":"Arsenic-related microorganisms in groundwater: A review on distribution, metabolic activities and potential use in arsenic removal processes","volume":"16","author":"Crognale","year":"2017","journal-title":"Rev. Environ. Sci. Bio-Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e0176921","DOI":"10.1128\/AEM.01769-21","article-title":"Diversity and Metabolic Potentials of As(III)-Oxidizing Bacteria in Activated Sludge","volume":"87","author":"Xu","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"122268","DOI":"10.1016\/j.envres.2025.122268","article-title":"Soil antimony-microbe interactions in an abandoned antimony mine in southern China","volume":"284","author":"Pan","year":"2025","journal-title":"Environ. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"180608","DOI":"10.1016\/j.scitotenv.2025.180608","article-title":"Bacterial interactions with arsenic: Metabolic pathways, resistance mechanisms, and bioremediation approaches","volume":"1002","author":"Kushwaha","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1007\/s00128-021-03118-6","article-title":"Spatial Distribution and Environmental Risk of Arsenic and Antimony in Soil Around an Antimony Smelter of Qinglong County","volume":"107","author":"He","year":"2021","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1023\/A:1009255012328","article-title":"Oxidation of arsenite to arsenate by a bacterium isolated from an aquatic environment","volume":"12","author":"Weeger","year":"1999","journal-title":"Biometals"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/S0584-8547(03)00036-3","article-title":"Redox speciation analysis of antimony in soil extracts by hydride generation atomic fluorescence spectrometry","volume":"58","author":"Fuentes","year":"2003","journal-title":"Spectroc. Acta Pt. B-Atom. Spectr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0003-2670(01)00924-2","article-title":"Arsenic fractionation in soils using an improved sequential extraction procedure","volume":"436","author":"Wenzel","year":"2001","journal-title":"Anal. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Magoc","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Meth."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.envpol.2017.01.057","article-title":"Antimony as a global dilemma: Geochemistry, mobility, fate and transport","volume":"223","author":"Herath","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3720","DOI":"10.1080\/10643389.2021.1944588","article-title":"Stabilization and encapsulation of arsenic-\/antimony-bearing mine waste: Overview and outlook of existing techniques","volume":"52","author":"Alvarez","year":"2022","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"120040","DOI":"10.1016\/j.envpol.2022.120040","article-title":"Characterization of arsenic-metabolizing bacteria in an alkaline soil","volume":"312","author":"Zhang","year":"2022","journal-title":"Environ. Pollut."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"121561","DOI":"10.1016\/j.jhazmat.2019.121561","article-title":"Mechanism of microbial dissolution and oxidation of antimony in stibnite under ambient conditions","volume":"385","author":"Loni","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"134156","DOI":"10.1016\/j.jhazmat.2024.134156","article-title":"Antimony and arsenic migration in a heterogeneous subsurface at an abandoned antimony smelter under rainfall","volume":"470","author":"Li","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"139979","DOI":"10.1016\/j.jhazmat.2025.139979","article-title":"Release and immobilization of arsenic and antimony in contaminated soil system during sulfidation and reoxidation cycling","volume":"498","author":"Zhang","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"133731","DOI":"10.1016\/j.jhazmat.2024.133731","article-title":"Stibnite dissolution and Sb oxidation by Paraccocus versutus XT0.6 via direct and indirect contact","volume":"467","author":"Li","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jes.2021.03.042","article-title":"Antimony transformation and mobilization from stibnite by an antimonite oxidizing bacterium Bosea sp. AS-1","volume":"111","author":"Xiang","year":"2022","journal-title":"J. Environ. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"138141","DOI":"10.1016\/j.jhazmat.2025.138141","article-title":"Distinct mechanisms of stibnite (Sb2S3) oxidative dissolution mediated by acid-generating and alkali-generating microorganisms within a wide pH range","volume":"492","author":"Ma","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s10653-010-9284-z","article-title":"Antimony speciation and contamination of waters in the Xikuangshan antimony mining and smelting area, China","volume":"32","author":"Liu","year":"2010","journal-title":"Environ. Geochem. Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0012-8252(01)00070-8","article-title":"Antimony in the environment: A review focused on natural waters I. Occurrence","volume":"57","author":"Filella","year":"2002","journal-title":"Earth-Sci. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jes.2024.01.016","article-title":"A critical review on arsenic and antimony adsorption and transformation on mineral facets","volume":"153","author":"Kumar","year":"2025","journal-title":"J. Environ. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.gca.2022.07.017","article-title":"Precipitation of arsenic-bearing solids as a secondary control on arsenic speciation in groundwater: Evidence from field study and geochemical analysis","volume":"333","author":"Nilling","year":"2022","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0304-3894(99)00105-3","article-title":"The stabilities of calcium arsenates at 23 \u00b1 1 \u00b0C","volume":"69","author":"Bothe","year":"1999","journal-title":"J. Hazard. Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11447","DOI":"10.1021\/acs.est.4c00863","article-title":"Differential Mechanisms of Microbial As(III) and Sb(III) Oxidation and Their Contribution to Tailings Reclamation","volume":"58","author":"Kong","year":"2024","journal-title":"Environ. Sci. Technol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Li, C., Zong, G., Chen, X., Tan, M., Gao, W., Fu, J., Zhang, P., Wang, B., and Cao, G. (2023). Bifunctional protein ArsRM contributes to arsenite methylation and resistance in Brevundimonas sp. M20. BMC Microbiol., 23.","DOI":"10.1186\/s12866-023-02876-z"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1071\/EN14123","article-title":"Antimony leaching from contaminated soil under manganese- and iron-reducing conditions: Column experiments","volume":"11","author":"Hockmann","year":"2014","journal-title":"Environ. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jenvman.2018.02.060","article-title":"Enhancing arsenic removal from arsenic-contaminated water by Echinodorus cordifolius-endophytic Arthrobacter creatinolyticus interactions","volume":"213","author":"Prum","year":"2018","journal-title":"J. Environ. Manag."}],"container-title":["Toxics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2305-6304\/14\/9\/759\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:08:49Z","timestamp":1787749729000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2305-6304\/14\/9\/759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":46,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["toxics14090759"],"URL":"https:\/\/doi.org\/10.3390\/toxics14090759","relation":{},"ISSN":["2305-6304"],"issn-type":[{"value":"2305-6304","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}