{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:06:12Z","timestamp":1791399972651,"version":"4.3.3"},"reference-count":35,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000203","name":"U.S. Geological Survey","doi-asserted-by":"publisher","award":["G16AP00065"],"award-info":[{"award-number":["G16AP00065"]}],"id":[{"id":"10.13039\/100000203","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>Much of the seafood that humans consume comes from estuaries and coastal areas where microplastics (MPs) accumulate, due in part to continual input and degradation of plastic litter from rivers and runoff. As filter feeders, oysters (Crassostrea virginica) are especially vulnerable to MP pollution. In this study, we assessed MP pollution in water at oyster reefs along the Mississippi Gulf Coast when: (1) historic flooding of the Mississippi River caused the Bonnet Carr\u00e9 Spillway to remain open for a record period of time causing major freshwater intrusion to the area and deleterious impacts on the species and (2) the spillway was closed, and normal salinity conditions resumed. Microplastics (~25 \u00b5m\u20135 mm) were isolated using a single-pot method, preparing samples in the same vessel (Mason jars) used for their collection right up until the MPs were transferred onto filters for analyses. The MPs were quantified using Nile Red fluorescence detection and identified using laser direct infrared (LDIR) analysis. Concentrations ranged from ~12 to 381 particles\/L and tended to decrease at sites impacted by major freshwater intrusion. With the spillway open, average MP concentrations were positively correlated with salinity (r = 0.87, p = 0.05) for sites with three or more samples examined. However, the dilution effect on MP abundances was temporary, and oyster yields suffered from the extended periods of lower salinity. There were no significant changes in the relative distribution of MPs during freshwater intrusions; most of the MPs (&gt;50%) were in the lower size fraction (~25\u201390 \u00b5m) and consisted mostly of fragments (~84%), followed by fibers (~11%) and beads (~5%). The most prevalent plastic was polyester, followed by acrylates\/polyurethanes, polyamide, polypropylene, polyethylene, and polyacetal. Overall, this work provides much-needed empirical data on the abundances, morphologies, and types of MPs that oysters are exposed to in the Mississippi Sound, although how much of these MPs are ingested and their impacts on the organisms deserves further scrutiny. This paper is believed to be the first major application of LDIR to the analysis of MPs in natural waters.<\/jats:p>","DOI":"10.3390\/toxics8020035","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:37:38Z","timestamp":1589971058000},"page":"35","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":149,"title":["Occurrence of Microplastic Pollution at Oyster Reefs and Other Coastal Sites in the Mississippi Sound, USA: Impacts of Freshwater Inflows from Flooding"],"prefix":"10.3390","volume":"8","author":[{"given":"Austin","family":"Scircle","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James V.","family":"Cizdziel","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Tisinger","sequence":"additional","affiliation":[{"name":"Agilent Technologies Inc., 2850 Centerville Road, Wilmington, DE 19808, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun","family":"Anumol","sequence":"additional","affiliation":[{"name":"Agilent Technologies Inc., 2850 Centerville Road, Wilmington, DE 19808, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren","family":"Robey","sequence":"additional","affiliation":[{"name":"Agilent Technologies Inc., 2850 Centerville Road, Wilmington, DE 19808, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/j.marpolbul.2011.05.030","article-title":"Microplastics in the marine environment","volume":"62","author":"Andrady","year":"2011","journal-title":"Mar. 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