{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T06:00:51Z","timestamp":1783663251336,"version":"3.55.0"},"reference-count":64,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009469","name":"Conseil r\u00e9gional du Centre-Val de Loire","doi-asserted-by":"publisher","award":["32000838"],"award-info":[{"award-number":["32000838"]}],"id":[{"id":"10.13039\/501100009469","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxics"],"abstract":"<jats:p>The effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg\u22121 day\u22121 glyphosate) for 5 weeks in 32-week-old roosters (n = 5 RU-exposed and n = 5 control (CT)). Although the concentrations of glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) increased in blood plasma and seminal fluid during exposure, no significant differences in testis weight and sperm concentrations were observed between RU and CT roosters. However, sperm motility was significantly reduced, associated with decreased calcium and ATP concentrations in RU spermatozoa. Plasma testosterone and oestradiol concentrations increased in RU roosters. These negative effects ceased 14 days after RU removal from the diet. Epigenetic analysis showed a global DNA hypomethylation in RU roosters. After artificial insemination of hens (n = 40) with sperm from CT or RU roosters, eggs were collected and artificially incubated. Embryo viability did not differ, but chicks from RU roosters (n = 118) had a higher food consumption, body weight and subcutaneous adipose tissue content. Chronic dietary RU exposure in roosters reduces sperm motility and increases plasma testosterone levels, growth performance, and fattening in offspring.<\/jats:p>","DOI":"10.3390\/toxics9120318","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T03:12:40Z","timestamp":1638328360000},"page":"318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5386-2435","authenticated-orcid":false,"given":"Lo\u00efse","family":"Serra","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Estienne","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Bourdon","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Ram\u00e9","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Chevaleyre","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Didier","sequence":"additional","affiliation":[{"name":"Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement\u2014Unit\u00e9 Exp\u00e9rimentale du P\u00f4le d\u2019Exp\u00e9rimentation Avicole de Tours UEPEAT 1295, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine","family":"Chahnamian","sequence":"additional","affiliation":[{"name":"Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement\u2014Unit\u00e9 Exp\u00e9rimentale du P\u00f4le d\u2019Exp\u00e9rimentation Avicole de Tours UEPEAT 1295, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souleiman","family":"El Balkhi","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie, Toxicologie et Pharmacovigilance, CHU, F-87042 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-9598","authenticated-orcid":false,"given":"Pascal","family":"Froment","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00eblle","family":"Dupont","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut Fran\u00e7ais du Cheval et de l\u2019Equitation, Institut National de Recherche pour l\u2019Agriculture, l\u2019Alimentation et l\u2019Environnement, Universit\u00e9 de Tours, PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135808","DOI":"10.1016\/j.scitotenv.2019.135808","article-title":"Effects of glyphosate exposure on human health: Insights from epidemiological and in vitro studies","volume":"705","author":"Agostini","year":"2020","journal-title":"Sci. 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