{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:46:37Z","timestamp":1789908397983,"version":"4.0.1"},"reference-count":56,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T00:00:00Z","timestamp":1524700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 2011\/23236-4 to S.V.S."],"award-info":[{"award-number":["# 2011\/23236-4 to S.V.S."]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 2015\/26609-7 to F.G.A"],"award-info":[{"award-number":["# 2015\/26609-7 to F.G.A"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 2016\/20641-9 to F.G.A"],"award-info":[{"award-number":["# 2016\/20641-9 to F.G.A"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 2015\/00740-0 to T.R.C."],"award-info":[{"award-number":["# 2015\/00740-0 to T.R.C."]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FEDER and the Walloon region funding.","award":["Supported GIGA Proteomics Facility"],"award-info":[{"award-number":["Supported GIGA Proteomics Facility"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes\u2019 age, habitat, diet, and sexual dimorphism. The present study reports the first in-depth investigation of venom composition in male and female Bothrops moojeni (B. moojeni) snakes (BmooM and BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, and immunoreactivity characterization. Compared with BmooM venom, BmooF venom exhibited weaker hyaluronidase, metalloproteinase, and phospholipase activity; stronger recognition by anti-bothropic serum; 1.4-fold stronger cytotoxicity; and greater number of peptides. The increased L-amino acid oxidase expression probably accounted for the stronger immunoreactivity and cytotoxicity of BmooF venom. BmooF and BmooM venom shared only 19% peptides. Some venom components were gender-specific, such as phospholipases B, phospholipase inhibitor, and hyaluronidases in BmooM, and cysteine-rich secretory proteins in BmooF. In conclusion, we describe herein the first proteomics study of B. moojeni snake venom and an in-depth characterization of gender-specific differences in venom composition. Altogether, our findings not only stress the importance of considering the snake\u2019s gender during antivenom production, but also help to identify new potential drugs and biotechnological tools.<\/jats:p>","DOI":"10.3390\/toxins10050177","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T06:52:23Z","timestamp":1524811943000},"page":"177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1453-3185","authenticated-orcid":false,"given":"Fernanda Gobbi","family":"Amorim","sequence":"first","affiliation":[{"name":"Laboratory of Toxinology, Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo\u2014USP, Ribeir\u00e3o Preto, SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tassia Rafaela","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Toxinology, Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo\u2014USP, Ribeir\u00e3o Preto, SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-0118","authenticated-orcid":false,"given":"Dominique","family":"Baiwir","sequence":"additional","affiliation":[{"name":"GIGA Proteomics Facility, GIGA Institute, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"De Pauw","sequence":"additional","affiliation":[{"name":"Laboratory of Mass Spectrometry, Department of Chemistry, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8153-9590","authenticated-orcid":false,"given":"Loic","family":"Quinton","sequence":"additional","affiliation":[{"name":"Laboratory of Mass Spectrometry, Department of Chemistry, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suely Vilela","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Laboratory of Toxinology, Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo\u2014USP, Ribeir\u00e3o Preto, SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/j.febslet.2009.03.029","article-title":"Venoms, venomics, antivenomics","volume":"583","author":"Calvete","year":"2009","journal-title":"FEBS Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.3390\/toxins6123488","article-title":"Quo vadis venomics? A roadmap to neglected venomous invertebrates","volume":"6","author":"Campbell","year":"2014","journal-title":"Toxins (Basel)"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40409-017-0127-6","article-title":"Snakebite envenomation turns again into a neglected tropical disease!","volume":"23","author":"Chippaux","year":"2017","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1007\/s00204-015-1533-6","article-title":"Moojenactivase, a novel pro-coagulant piiid metalloprotease isolated from bothrops moojeni snake venom, activates coagulation factors ii and x and induces tissue factor up-regulation in leukocytes","volume":"90","author":"Sartim","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chippaux, J.P. (2017). Incidence and mortality due to snakebite in the americas. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005662"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.toxicon.2016.03.006","article-title":"Comparative analysis of local effects caused by bothrops alternatus and bothrops moojeni snake venoms: Enzymatic contributions and inflammatory modulations","volume":"117","author":"Mamede","year":"2016","journal-title":"Toxicon"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1670\/120-02A","article-title":"Ecology of the pitviper, bothrops moojeni, in the brazilian cerrado","volume":"37","author":"Nogueira","year":"2003","journal-title":"J. Herpetol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.4155\/fmc.14.99","article-title":"Cone snail venomics: From novel biology to novel therapeutics","volume":"6","author":"Prashanth","year":"2014","journal-title":"Future Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1042\/BCJ20160577","article-title":"Venomics: Integrative venom proteomics and beyond","volume":"474","author":"Calvete","year":"2017","journal-title":"Biochem. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40409-017-0117-8","article-title":"Strategies in \u2018snake venomics\u2019 aiming at an integrative view of compositional, functional, and immunological characteristics of venoms","volume":"23","author":"Lomonte","year":"2017","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zancolli, G., Baker, T.G., Barlow, A., Bradley, R.K., Calvete, J.J., Carter, K.C., de Jager, K., Owens, J.B., Price, J.F., and Sanz, L. (2016). Is hybridization a source of adaptive venom variation in rattlesnakes? A test, using a crotalus scutulatus \u00d7 viridis hybrid zone in southwestern new mexico. Toxins (Basel), 8.","DOI":"10.3390\/toxins8060188"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4585","DOI":"10.1021\/pr4007393","article-title":"Individual variability in the venom proteome of juvenile bothrops jararaca specimens","volume":"12","author":"Dias","year":"2013","journal-title":"J. Proteome Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jprot.2015.04.029","article-title":"Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two bothrops jararaca populations from geographic isolated regions within the brazilian atlantic rainforest","volume":"135","author":"Pla","year":"2016","journal-title":"J. Proteom."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jprot.2017.03.003","article-title":"Functional proteomic analyses of bothrops atrox venom reveals phenotypes associated with habitat variation in the amazon","volume":"159","author":"Sousa","year":"2017","journal-title":"J. Proteom."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0041-0101(91)90106-2","article-title":"Comparative study of nine bothrops snake venoms from adult female snakes and their offspring","volume":"29","author":"Furtado","year":"1991","journal-title":"Toxicon"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1021\/pr901027r","article-title":"Analysis of the ontogenetic variation in the venom proteome\/peptidome of bothrops jararaca reveals different strategies to deal with prey","volume":"9","author":"Zelanis","year":"2010","journal-title":"J. Proteom. Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zelanis, A., Andrade-Silva, D., Rocha, M.M., Furtado, M.F., Serrano, S.M., Junqueira-de-Azevedo, I.L., and Ho, P.L. (2012). A transcriptomic view of the proteome variability of newborn and adult bothrops jararaca snake venoms. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001554"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/j.toxicon.2010.08.011","article-title":"Comparative analysis of newborn and adult bothrops jararaca snake venoms","volume":"56","author":"Antunes","year":"2010","journal-title":"Toxicon"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1002\/pmic.201100287","article-title":"Bothrops jararaca venom proteome rearrangement upon neonate to adult transition","volume":"11","author":"Zelanis","year":"2011","journal-title":"Proteomics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1002\/rcm.2931","article-title":"Mass spectrometric analysis of the individual variability of bothrops jararaca venom peptide fraction. Evidence for sex-based variation among the bradykinin-potentiating peptides","volume":"21","author":"Pimenta","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.toxicon.2005.11.007","article-title":"Sex-based individual variation of snake venom proteome among eighteen bothrops jararaca siblings","volume":"47","author":"Menezes","year":"2006","journal-title":"Toxicon"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jprot.2016.02.030","article-title":"Proteomic identification of gender molecular markers in bothrops jararaca venom","volume":"139","author":"Zelanis","year":"2016","journal-title":"J. Proteom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.toxicon.2006.06.005","article-title":"Sexual dimorphism in venom of bothrops jararaca(serpentes: Viperidae)","volume":"48","author":"Furtado","year":"2006","journal-title":"Toxicon"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43237","DOI":"10.1038\/srep43237","article-title":"Venomics of tropidolaemus wagleri, the sexually dimorphic temple pit viper: Unveiling a deeply conserved atypical toxin arsenal","volume":"7","author":"Tan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1590\/S0037-86822010000600011","article-title":"Comparison of bothropoides jararaca bites with and without envenoming treated at the vital brazil hospital of the butantan institute, state of s\u00e3o paulo, brazil","volume":"43","author":"Nicoleti","year":"2010","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1590\/S0036-46651989000200004","article-title":"Acidentes of\u00eddicos causados por bothrops moojeni: Correla\u00e7\u00e4o do quadro cl\u00ednico com o tamanho da serpente","volume":"31","author":"Kouyoumdjian","year":"1989","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1590\/S0036-46651988000600007","article-title":"Acidentes of\u00eddicos causados por bothrops moojeni: Relato de 37 casos","volume":"30","author":"Kouyoumdjian","year":"1988","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Melani, R.D., Goto-Silva, L., Nogueira, F.C.S., Junqueira, M., and Domont, G.B. (2016). Shotgun approaches for venom analysis. Venom Genomics and Proteomics, Springer.","DOI":"10.1007\/978-94-007-6416-3_26"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.toxicon.2017.10.025","article-title":"New findings from the first transcriptome of the bothrops moojeni snake venom gland","volume":"140","author":"Amorim","year":"2017","journal-title":"Toxicon"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"383","DOI":"10.2307\/1563568","article-title":"Ecology of the australian elapid snake tropidechis carinatus","volume":"16","author":"Shine","year":"1982","journal-title":"J. Herpetol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.toxicon.2013.01.006","article-title":"Individual venom profiling of crotalus durissus terrificus specimens from a geographically limited region: Crotamine assessment and captivity evaluation on the biological activities","volume":"69","author":"Pimenta","year":"2013","journal-title":"Toxicon"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.1021\/acs.jproteome.6b00561","article-title":"Dynamic rearrangement in snake venom gland proteome: Insights into bothrops jararaca intraspecific venom variation","volume":"15","author":"Stuginski","year":"2016","journal-title":"J. Proteom. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1641\/0006-3568(2002)052[1121:DSMV]2.0.CO;2","article-title":"Do snakes meter venom?","volume":"52","author":"Young","year":"2002","journal-title":"BioScience"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"Warrell","year":"2010","journal-title":"Lancet"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.toxicon.2017.04.015","article-title":"\u201cDry bite\u201d In venomous snakes: A review","volume":"133","author":"Naik","year":"2017","journal-title":"Toxicon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40409-015-0025-8","article-title":"Bordonein-L, a new L-amino acid oxidase from crotalus durissus terrificus snake venom: Isolation, preliminary characterization and enzyme stability","volume":"21","author":"Bordon","year":"2015","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/S0041-0101(02)00102-2","article-title":"Snake venom L-amino acid oxidases","volume":"40","author":"Du","year":"2002","journal-title":"Toxicon"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-1-61779-600-5_4","article-title":"Phospholipases: An overview","volume":"861","author":"Aloulou","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.bbagen.2016.12.022","article-title":"Minor snake venom proteins: Structure, function and potential applications","volume":"1861","author":"Cologna","year":"2017","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0006-291X(03)00155-4","article-title":"Structural and functional analysis of bmjmip, a phospholipase a2 myotoxin inhibitor protein from bothrops moojeni snake plasma","volume":"302","author":"Soares","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zainal Abidin, S.A., Rajadurai, P., Chowdhury, M.E., Ahmad Rusmili, M.R., Othman, I., and Naidu, R. (2016). Proteomic characterization and comparison of malaysian tropidolaemus wagleri and cryptelytrops purpureomaculatus venom using shotgun-proteomics. Toxins (Basel), 8.","DOI":"10.3390\/toxins8100299"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1021\/cr3003533","article-title":"Protein analysis by shotgun\/bottom-up proteomics","volume":"113","author":"Zhang","year":"2013","journal-title":"Chem. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/10890","article-title":"Direct analysis of protein complexes using mass spectrometry","volume":"17","author":"Link","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1074\/mcp.M113.034769","article-title":"The one hour yeast proteome","volume":"13","author":"Hebert","year":"2014","journal-title":"Mol. Cell. Proteom."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Thakur, S.S., Geiger, T., Chatterjee, B., Bandilla, P., Fr\u00f6hlich, F., Cox, J., and Mann, M. (2011). Deep and highly sensitive proteome coverage by lc-ms\/ms without prefractionation. Mol. Cell. Proteom., 10.","DOI":"10.1074\/mcp.M110.003699"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jprot.2013.11.001","article-title":"Linking the transcriptome and proteome to characterize the venom of the eastern diamondback rattlesnake (crotalus adamanteus)","volume":"96","author":"Margres","year":"2014","journal-title":"J. Proteom."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/0041-0101(88)90245-0","article-title":"The hyaluronidase activities of some southeast asian snake venoms","volume":"26","author":"Pukrittayakamee","year":"1988","journal-title":"Toxicon"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1016\/j.biochi.2012.08.014","article-title":"Isolation, enzymatic characterization and antiedematogenic activity of the first reported rattlesnake hyaluronidase from crotalus durissus terrificus venom","volume":"94","author":"Bordon","year":"2012","journal-title":"Biochimie"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.bbrc.2004.09.031","article-title":"A novel p-i class metalloproteinase with broad substrate-cleaving activity, agkislysin, from agkistrodon acutus venom","volume":"324","author":"Wang","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0041-0101(88)90010-4","article-title":"An alternative in vitro method for testing the potency of the polyvalent antivenom produced in costa rica","volume":"26","author":"Avila","year":"1988","journal-title":"Toxicon"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1139\/y59-157","article-title":"A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin","volume":"37","author":"Hummel","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ijbiomac.2015.07.004","article-title":"Evaluating the microbicidal, antiparasitic and antitumor effects of cr-laao from calloselasma rhodostoma venom","volume":"80","author":"Costa","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0022-2836(70)90402-X","article-title":"A factor preventing the major head protein of bacteriophage t4 from random aggregation","volume":"47","author":"Laemmli","year":"1970","journal-title":"J. Mol. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.jprot.2013.01.021","article-title":"Proteomic analysis of bothrops pirajai snake venom and characterization of bpirmp, a new P-I metalloproteinase","volume":"80","author":"Bernardes","year":"2013","journal-title":"J. Proteom."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1002\/rcm.1196","article-title":"Peaks: Powerful software for peptide de novo sequencing by tandem mass spectrometry","volume":"17","author":"Ma","year":"2003","journal-title":"Rapid Commun. Mass Spectrom."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/10\/5\/177\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:02:16Z","timestamp":1760194936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/10\/5\/177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,26]]},"references-count":56,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2018,5]]}},"alternative-id":["toxins10050177"],"URL":"https:\/\/doi.org\/10.3390\/toxins10050177","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,26]]}}}