{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:09:25Z","timestamp":1790672965924,"version":"4.1.0"},"reference-count":90,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T00:00:00Z","timestamp":1528243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian National Institute of Science and Technology (INCT-Mar-COI)","award":["610012\/2011-8"],"award-info":[{"award-number":["610012\/2011-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Dinophysis spp. are a major source of diarrheic toxins to marine food webs, especially during blooms. This study documented the occurrence, in late May 2016, of a massive toxic bloom of the Dinophysis acuminata complex along the southern coast of Brazil, associated with an episode of marked salinity stratification. The study tracked the daily vertical distribution of Dinophysis spp. cells and their ciliate prey, Mesodinium cf. rubrum, and quantified the amount of lipophilic toxins present in seston and accumulated by various marine organisms in the food web. The abundance of the D. acuminata complex reached 43 \u00d7 104 cells\u00b7L\u22121 at 1.0 m depth at the peak of the bloom. Maximum cell densities of cryptophyceans and M. cf. rubrum (&gt;500 \u00d7 104 and 18 \u00d7 104 cell\u00b7L\u22121, respectively) were recorded on the first day of sampling, one week before the peak in abundance of the D. acuminata complex. The diarrheic toxin okadaic acid (OA) was the only toxin detected during the bloom, attaining unprecedented, high concentrations of up to 829 \u00b5g\u00b7L\u22121 in seston, and 143 \u00b1 93 pg\u00b7cell\u22121 in individually picked cells of the D. acuminata complex. Suspension-feeders such as the mussel, Perna perna, and barnacle, Megabalanus tintinnabulum, accumulated maximum OA levels (up to 578.4 and 21.9 \u00b5g total OA\u00b7Kg\u22121, respectively) during early bloom stages, whereas predators and detritivores such as Caprellidae amphipods (154.6 \u00b5g\u00b7Kg\u22121), Stramonita haemastoma gastropods (111.6 \u00b5g\u00b7Kg\u22121), Pilumnus spinosissimus crabs (33.4 \u00b5g\u00b7Kg\u22121) and a commercially important species of shrimp, Xiphopenaeus kroyeri (7.2 \u00b5g\u00b7Kg\u22121), only incorporated OA from mid- to late bloom stages. Conjugated forms of OA were dominant (&gt;70%) in most organisms, except in blenny fish, Hypleurochilus fissicornis, and polychaetes, Pseudonereis palpata (up to 59.3 and 164.6 \u00b5g total OA\u00b7Kg\u22121, respectively), which contained mostly free-OA throughout the bloom. Although algal toxins are only regulated in bivalves during toxic blooms in most countries, including Brazil, this study indicates that human seafood consumers might be exposed to moderate toxin levels from a variety of other vectors during intense toxic outbreaks.<\/jats:p>","DOI":"10.3390\/toxins10060232","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T10:53:28Z","timestamp":1528282408000},"page":"232","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Diel Variations in Cell Abundance and Trophic Transfer of Diarrheic Toxins during a Massive Dinophysis Bloom in Southern Brazil"],"prefix":"10.3390","volume":"10","author":[{"given":"Thiago Pereira","family":"Alves","sequence":"first","affiliation":[{"name":"Center for Marine Studies, Federal University of Paran\u00e1. Av. Beira-mar s\/n, P.O. Box: 61, Pontal do Paran\u00e1 PR 83255-976, Brazil"},{"name":"Federal Institute of Santa Catarina, Av. Ver. Abra\u00e3o Jo\u00e3o Francisco, 3988, Ressacada, Itaja\u00ed SC 88307-303, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Luiz Laureno","family":"Mafra","sequence":"additional","affiliation":[{"name":"Center for Marine Studies, Federal University of Paran\u00e1. Av. Beira-mar s\/n, P.O. Box: 61, Pontal do Paran\u00e1 PR 83255-976, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.hal.2011.10.016","article-title":"Harmful Dinophysis species: A review","volume":"14","author":"Reguera","year":"2012","journal-title":"Harmful Algae"},{"key":"ref_2","first-page":"19","article-title":"Bloom of Dinophysis spp. dominated by D. sacculus and its related diarrhetic shellfish poisoning (DSP) outbreak in Alfacs Bay (Catalonia, NW Mediterranean Sea): Identification of DSP toxins in phytoplankton, shellfish and passive samplers","volume":"6","author":"Casanova","year":"2016","journal-title":"Reg. Stud. Mar. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.hal.2014.09.006","article-title":"Changing wind patterns linked to unusually high Dinophysis blooms around the Shetland Islands, Scotland","volume":"39","author":"Whyte","year":"2014","journal-title":"Harmful Algae"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"273","DOI":"10.2989\/1814232X.2011.600372","article-title":"Accumulation of diarrhetic shellfish poisoning toxins in the oyster Crassostrea gigas and the mussel Choromytilus meridionalis in the southern Benguela ecosystem","volume":"33","author":"Pitcher","year":"2011","journal-title":"Afr. J. Mar. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.hal.2014.07.017","article-title":"First evidence of cell deformation occurrence during a Dinophysis bloom along the shores of the Gulf of Tunis (SW Mediterranean Sea)","volume":"39","author":"Aissaoui","year":"2014","journal-title":"Harmful Algae"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1007\/s11356-014-3595-z","article-title":"Lipophilic shellfish toxins in Dinophysis caudata picked cells and in shellfish from the East China Sea","volume":"22","author":"Li","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.hal.2013.03.005","article-title":"The emergence of Dinophysis acuminata blooms and DSP toxins in shellfish in New York waters","volume":"26","author":"Marcoval","year":"2013","journal-title":"Harmful Algae"},{"key":"ref_8","first-page":"19","article-title":"Intensification of marine dinoflagellates blooms in Uruguay Intensificaci\u00f3n de floraciones de dinoflagelados marinos en Uruguay Intensification of marine dinoflagellates blooms in Uruguay","volume":"13","author":"Nacional","year":"2017","journal-title":"Rev. Lab. Tecnol. Urug."},{"key":"ref_9","first-page":"5","article-title":"First Report of Diarrheic Shellfish Toxins in Mollusks from Buenos Aires Province (Argentina) associated with Dinophysis spp.: Evidence os okadaic acid, dinophysistoxin-1 and their acyl-derivates","volume":"47","author":"Sar","year":"2012","journal-title":"Biol. Soc. Argent. Bot."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.2983\/035.034.0339","article-title":"Dinophysis Species Associated with Diarrhetic Shellfish Poisoning Episodes in North Patagonian Gulfs (Chubut, Argentina)","volume":"34","author":"Villalobos","year":"2015","journal-title":"J. Shellfish Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.hal.2008.08.018","article-title":"Complexity in the eutrophication\u2013harmful algal bloom relationship, with comment on the importance of grazing","volume":"8","author":"Smayda","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.hal.2008.08.012","article-title":"Lagoonal stormwater detention ponds as promoters of harmful algal blooms and eutrophication along the South Carolina coast","volume":"8","author":"Lewitus","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.hal.2008.08.006","article-title":"Eutrophication and harmful algal blooms: A scientific consensus","volume":"8","author":"Heisler","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.hal.2008.08.009","article-title":"How does eutrophication affect the role of grazers in harmful algal bloom dynamics?","volume":"8","author":"Buskey","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/j.1529-8817.2010.00815.x","article-title":"Ocean climate change, phytoplankton community responses, and harmful algal blooms: A formidable predictive challenge","volume":"46","author":"Hallegraeff","year":"2010","journal-title":"J. Phycol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.hal.2011.10.027","article-title":"The rise of harmful cyanobacteria blooms: The potential roles of eutrophication and climate change","volume":"14","author":"Davis","year":"2012","journal-title":"Harmful Algae"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1590\/S1676-06032010000300011","article-title":"Dinophysiales (Dinophyceae) no extremo Sul do Brasil (inverno de 2005, ver\u00e3o de 2007)","volume":"10","author":"Haraguchi","year":"2010","journal-title":"Biota Neotrop."},{"key":"ref_18","unstructured":"Proen\u00e7a, L.A.O., Schramm, M.A., da Silva Tamanaha, M., and Alves, T.P. (2007). Diarrhoetic shellfish poisoning (DSP) outbreak in Subtropical Southwest Atlantic. Harmful Algae News, 1\u201328."},{"key":"ref_19","first-page":"570","article-title":"Perfil epidemiologico de surtos de DTA por moluscos bivalves em Santa Catarina, Brasil, em 2007 e 2008","volume":"23","author":"Rosa","year":"2009","journal-title":"Hig. Aliment."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3920","DOI":"10.3390\/md13063920","article-title":"Persistent Contamination of Octopuses and Mussels with Lipophilic Shellfish Toxins during Spring Dinophysis Blooms in a Subtropical Estuary","volume":"13","author":"Mafra","year":"2015","journal-title":"Mar. Drugs."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/s12562-015-0882-7","article-title":"Differential okadaic acid accumulation and detoxification by oysters and mussels during natural and simulated Dinophysis blooms","volume":"81","author":"Mafra","year":"2015","journal-title":"Fish. Sci."},{"key":"ref_22","first-page":"459","article-title":"Evidences of diarrhetic shellfish poisoning in Santa Catarina\u2014Brazil","volume":"50","author":"Schmitt","year":"1998","journal-title":"J. Braz. Assoc. Advant. Sci."},{"key":"ref_23","unstructured":"BRASIL (2012). Instru\u00e7\u00e3o Normativa Interministerial 7 de 8 de Maio de 2012."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3390\/md12010394","article-title":"Dinophysis toxins: Causative organisms, distribution and fate in shellfish","volume":"12","author":"Reguera","year":"2014","journal-title":"Mar. Drugs."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.hal.2015.12.002","article-title":"Toward predicting Dinophysis blooms off NW Iberia: A decade of events","volume":"53","author":"Moita","year":"2016","journal-title":"Harmful Algae"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1111\/j.1529-8817.2012.01150.x","article-title":"Dinophysis caudata (dinophyceae) sequesters and retains plastids from the mixotrophic ciliate prey Mesodinium rubrum","volume":"48","author":"Kim","year":"2012","journal-title":"J. Phycol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.hal.2016.07.001","article-title":"The use of a mucus trap by Dinophysis acuta for the capture of Mesodinium rubrum prey under culture conditions","volume":"58","author":"Beuzenberg","year":"2016","journal-title":"Harmful Algae"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.hal.2016.09.009","article-title":"Harmful effects of Dinophysis to the ciliate Mesodinium rubrum: Implications for prey capture","volume":"59","author":"Mafra","year":"2016","journal-title":"Harmful Algae"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.hal.2016.02.001","article-title":"Mass entrapment and lysis of Mesodinium rubrum cells in mucus threads observed in cultures with Dinophysis","volume":"55","author":"Hansen","year":"2016","journal-title":"Harmful Algae"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.toxicon.2007.05.008","article-title":"Trophic transfer of paralytic shellfish toxins from the cladoceran (Moina mongolica) to larvae of the fish (Sciaenops ocellatus)","volume":"50","author":"Jiang","year":"2007","journal-title":"Toxicon"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.hal.2007.08.001","article-title":"Okadaic acid accumulation in macrofilter feeders subjected to natural blooms of Dinophysis acuminata","volume":"7","author":"Reizopoulou","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1007\/s10811-013-0219-9","article-title":"Diarrheic toxins in field-sampled and cultivated Dinophysis spp. cells from southern Brazil","volume":"26","author":"Mafra","year":"2014","journal-title":"J. Appl. Phycol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1080\/00364827.2000.10414597","article-title":"The first observation of okadaic acid in flounder in the Baltic Sea","volume":"85","author":"Meriluoto","year":"2000","journal-title":"Sarsia."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/j.toxicon.2006.08.002","article-title":"Transfer and metabolism of paralytic shellfish poisoning from scallop (Chlamys nobilis) to spiny lobster (Panulirus stimpsoni)","volume":"48","author":"Jiang","year":"2006","journal-title":"Toxicon"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/S0041-0101(02)00095-8","article-title":"First confirmation of human diarrhoeic poisonings by okadaic acid esters after ingestion of razor clams (Solen marginatus) and green crabs (Carcinus maenas) in Aveiro lagoon, Portugal and detection of okadaic acid esters in phytoplankton","volume":"40","author":"Vale","year":"2002","journal-title":"Toxicon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/29906","article-title":"Did algal toxins cause monk seal mortality?","volume":"393","author":"Robinson","year":"1998","journal-title":"Nature"},{"key":"ref_37","unstructured":"Mafra, L.L., Nolli, P.K., Luz, L.F., Leal, J.G., Sobrinho, B.F., Pimenta, B., Juraczky, L., Gonzalez, A.R.M., and Mota, L.E. (2016, January 9\u201314). Okadaic acid contamination during an exceptionally massive Dinophysis cf. acuminata bloom in southern Brazil. Proceedings of the Abstracts of the 17th International Conference of Harmful Algae, Florian\u00f3polis, Brazil."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.hal.2010.09.001","article-title":"Seasonal and intratidal distribution of Dinophysis spp. in a Chilean fjord","volume":"10","author":"Molinet","year":"2011","journal-title":"Harmful Algae"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.dsr2.2013.01.014","article-title":"Seasonal variability of Dinophysis spp. and Protoceratium reticulatum associated to lipophilic shellfish toxins in a strongly stratified Chilean fjord","volume":"101","author":"Varela","year":"2014","journal-title":"Deep. Res. Part II Top. Stud. Oceanogr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9361","DOI":"10.1007\/s10661-013-3257-5","article-title":"Ben Seasonal dynamic and in situ division rates of the dominant Dinophysis species in Punic harbors of Carthage (Gulf of Tunis, South Mediterranean)","volume":"185","author":"Aissaoui","year":"2013","journal-title":"Environ. Monit. Assess."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1007\/s00300-015-1686-z","article-title":"The ciliate Mesodinium rubrum and its cryptophyte prey in Antarctic aquatic environments","volume":"38","author":"Bell","year":"2015","journal-title":"Polar Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"267","DOI":"10.3354\/ame013267","article-title":"Some observations on vertical distribution and migration of the phototrophic ciliate Mesodinium rubrum (=Myrionecta rubra) in a stratified brackish inlet","volume":"13","author":"Crawford","year":"1997","journal-title":"Aquat. Microb. Ecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1093\/plankt\/fbt028","article-title":"Seasonal dynamics of Mesodinium rubrum in Chesapeake Bay","volume":"35","author":"Johnson","year":"2013","journal-title":"J. Plankton Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.hal.2011.10.010","article-title":"The marine dinoflagellate genus Dinophysis can retain plastids of multiple algal origins at the same time","volume":"13","author":"Kim","year":"2012","journal-title":"Harmful Algae"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.hal.2014.07.013","article-title":"Diversity and plastid types in Dinophysis acuminata complex (Dinophyceae) in Scottish waters","volume":"39","author":"Stern","year":"2014","journal-title":"Harmful Algae"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Wisecaver, J.H., and Hackett, J.D. (2010). Transcriptome analysis reveals nuclear-encoded proteins for the maintenance of temporary plastids in the dinoflagellate Dinophysis acuminata. BMC Genom., 11.","DOI":"10.1186\/1471-2164-11-366"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.hal.2015.02.004","article-title":"Fate of green plastids in Dinophysis caudata following ingestion of the benthic ciliate Mesodinium coatsi: Ultrastructure and psbA gene","volume":"43","author":"Kim","year":"2015","journal-title":"Harmful Algae"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.hal.2006.08.007","article-title":"Toxicity of Dinophysis spp. in relation to population density and environmental conditions on the Swedish west coast","volume":"6","author":"Lindahl","year":"2007","journal-title":"Harmful Algae"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.dsr2.2013.06.021","article-title":"A review of the biophysical interactions relevant to the promotion of HABs in stratified systems: The case study of Ireland","volume":"101","author":"Raine","year":"2014","journal-title":"Deep. Res. Part II Top. Stud. Oceanogr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1093\/plankt\/fbh042","article-title":"Dinophysis blooms in Greek coastal waters (Thermaikos Gulf, NW Aegean Sea)","volume":"26","author":"Koukaras","year":"2004","journal-title":"J. Plankton Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.hal.2014.12.005","article-title":"Co-occurrence of Dinophysis tripos and pectenotoxins in Argentinean shelf waters","volume":"42","author":"Fabro","year":"2015","journal-title":"Harmful Algae"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1016\/j.csr.2008.03.012","article-title":"The effects of river discharge and seasonal winds on the shelf off southeastern South America","volume":"28","author":"Piola","year":"2008","journal-title":"Cont. Shelf Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1016\/j.csr.2007.02.013","article-title":"Space\u2013time variability of the Plata plume inferred from ocean color","volume":"28","author":"Piola","year":"2008","journal-title":"Cont. Shelf Res."},{"key":"ref_54","unstructured":"Proen\u00e7a, L.A.O., and Hallegraeff, G.M. (2016, January 9\u201314). Extreme abundant bloom of Dinophysis ovum associated to positive SST anomalies in Uruguay. Proceedings of the 17th International Conference of Harmful Algae, Florian\u00f3polis, Brazil."},{"key":"ref_55","unstructured":"Proen\u00e7a, L.A.O., and Hallegraeff, G.M. (2016, January 9\u201314). Characterization of Dinophysis ovum as the causative agent of the exceptional DSP event in Uruguay during 2015. Proceedings of the 17th International Conference on Harmful Algae, Florian\u00f3polis, Brazil."},{"key":"ref_56","first-page":"33","article-title":"Clorofila a do fitopl\u00e2ncton em seis enseadas utilizadas para o cultivo de moluscos bivalves no litoral de Santa Catarina","volume":"6","year":"2002","journal-title":"Braz. J. Aquat. Sci. Technol."},{"key":"ref_57","unstructured":"Ferreira, J.F., Besen, K., Wormsbecher, A.G., and Santos, R.F. (2006). Physical-Chemical Parameters of Seawater Mollusc Culture Sites in Santa Catarina\u2014Brazil. J. Coast. Res., 1122\u20131126."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.marpolbul.2011.10.015","article-title":"A comparison of integrated and discrete depth sampling for monitoring toxic species of Dinophysis","volume":"64","author":"Escalera","year":"2012","journal-title":"Mar. Pollut. Bull."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.hal.2011.11.003","article-title":"Scales characterising a high density thin layer of Dinophysis acuta Ehrenberg and its transport within a coastal jet","volume":"15","author":"Farrell","year":"2012","journal-title":"Harmful Algae"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2006.07.033","article-title":"Relative toxicity of dinophysistoxin-2 (DTX-2) compared with okadaic acid, based on acute intraperitoneal toxicity in mice","volume":"49","author":"Aune","year":"2007","journal-title":"Toxicon"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.toxicon.2006.11.013","article-title":"Identification of pectenotoxins in plankton, filter feeders, and isolated cells of a Dinophysis acuminata with an atypical toxin profile, from Chile","volume":"49","author":"Blanco","year":"2007","journal-title":"Toxicon"},{"key":"ref_62","unstructured":"Froese, R., and Pauly, D. (2018, January 25). FishBase. Available online: http:\/\/fishbase.org."},{"key":"ref_63","unstructured":"Menezes, N.A., and Figueiredo, J.L. (1985). Manual de Peixes Marinhos do Sudeste do Brasil. V. Teleostei (4), University of S\u00e3o Paulo."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0022-0981(91)90144-L","article-title":"Caprellid amphipods: fast food for the reproductively active","volume":"148","author":"Caine","year":"1991","journal-title":"J. Exp. Mar. Bio. Ecol."},{"key":"ref_65","unstructured":"Brasil (2012). Portaria No 204, de 28 de Junho de 2012."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/plankt\/fbl053","article-title":"Monitoring of HAB species in the Mediterranean Sea through molecular methods","volume":"29","author":"Penna","year":"2006","journal-title":"J. Plankton Res."},{"key":"ref_67","unstructured":"Higman, W.A., Algoet, M., Stubbs, B., and Lees, D. (2007, January 18\u201323). Overview of developments of the algal biotoxin monitoring programme in England, Scotland and Wales. Proceedings of the 6th International Conference on Molluscan Shellfish Safety, Blenheim, Marlborough, New Zealand."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.hal.2007.05.002","article-title":"Two decades of marine biotoxin monitoring in bivalves from Portugal (1986\u20132006): A review of exposure assessment","volume":"7","author":"Vale","year":"2008","journal-title":"Harmful Algae"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.scitotenv.2012.03.059","article-title":"Integrated coastal monitoring of a gas processing plant using native and caged mussels","volume":"426","author":"Brooks","year":"2012","journal-title":"Sci. Total Environ."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.3390\/toxins7041206","article-title":"Integrative monitoring of marine and freshwater harmful algae in Washington State for public health protection","volume":"7","author":"Trainer","year":"2015","journal-title":"Toxins."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1502-3931.1996.tb01659.x","article-title":"Miocene barnacle assemblages from southern Spain and their palaeoenvironmental significance","volume":"29","author":"Doyle","year":"1996","journal-title":"Lethaia"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.hal.2013.12.001","article-title":"Planktonic marine copepods and harmful algae","volume":"32","author":"Turner","year":"2014","journal-title":"Harmful Algae"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S1568-9883(02)00067-7","article-title":"Importance of copepod faecal pellets to the fate of the DSP toxins produced by Dinophysis spp.","volume":"1","author":"Maneiro","year":"2002","journal-title":"Harmful Algae"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.hal.2005.05.002","article-title":"Food selectivity and grazing impact on toxic Dinophysis spp. by copepods feeding on natural plankton assemblages","volume":"5","author":"Carlsson","year":"2006","journal-title":"Harmful Algae"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1007\/s10661-011-2324-z","article-title":"Occurrence of diarrhetic shellfish poisoning (DSP) toxins in clams (Ruditapes decussatus) from Tunis north lagoon","volume":"184","author":"Armi","year":"2012","journal-title":"Environ. Monit. Assess."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.aquaculture.2009.02.033","article-title":"The ecological role of bivalve shellfish aquaculture in the estuarine environment: A review with application to oyster and clam culture in West Coast (USA) estuaries","volume":"290","author":"Dumbauld","year":"2009","journal-title":"Aquaculture."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.toxicon.2014.07.017","article-title":"Lipophilic toxins in cultivated mussels (Mytilus galloprovincialis) from Baja California, Mexico","volume":"90","author":"Turner","year":"2014","journal-title":"Toxicon"},{"key":"ref_78","unstructured":"Food and Agriculture Organization of the United Nations (FAO), and World Health Organization (WHO) (2016). Technical Paper on Toxicity Equivalency Factors for Marine Biotoxins Associated with Bivalve Molluscs, WHO."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1590\/S0101-81752005000400034","article-title":"Biologia e pesca do camar\u00e3o sete-barbas Xiphopenaeus kroyeri (Heller) (Crustacea, Penaeidae), na Arma\u00e7\u00e3o do Itapocoroy, Penha, Santa Catarina, Brasil","volume":"22","author":"Branco","year":"2005","journal-title":"Rev. Bras. Zool."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Alves, T.P., Schramm, M.A., Proen\u00e7a, L.A.O., Pinto, T.O., and Mafra, L.L. (2018). Interannual variability in Dinophysis spp. abundance and toxin accumulation in farmed mussels (Perna perna) in a subtropical estuary. Environ. Monit. Assess.","DOI":"10.1007\/s10661-018-6699-y"},{"key":"ref_81","first-page":"83","article-title":"Avalia\u00e7\u00e3o da dispers\u00e3o espacial da pluma do estu\u00e1rio do rio Itaja\u00ed-A\u00e7u em diferentes per\u00edodos de descarga","volume":"7","author":"Schettini","year":"2003","journal-title":"Braz. J. Aquat. Sci. Technol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"99","DOI":"10.14210\/bjast.v3n1.p99-109","article-title":"Aspectos Hidrodin\u00e2micos da Enseada da Arma\u00e7\u00e3o de Itapocoroy, SC","volume":"3","author":"Schettini","year":"1999","journal-title":"Braz. J. Aquat. Sci. Technol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"93","DOI":"10.14210\/bjast.v9n2.p93-102","article-title":"Variabilidade temporal das caracter\u00edsticas oceanogr\u00e1ficas e ecol\u00f3gicas da regi\u00e3o de influ\u00eancia fluvial do rio Itaja\u00ed-A\u00e7u","volume":"9","author":"Schettini","year":"2005","journal-title":"Braz. J. Aquat. Sci. Technol."},{"key":"ref_84","unstructured":"(2017, May 07). EURLMB EU-Harmonised Standard Operating Procedure for Determination of Lipophilic Marine Biotoxins in Molluscs by LC-MS\/MS, Version 5. Available online: www.aesan.msps.es\/en\/CRLMB\/web\/home.shtml."},{"key":"ref_85","unstructured":"Edler, L., and Elbr\u00e4chter, M. (2010). The Uterm\u00f6hl method for quantitative phytoplankton analysis. Microsc. Mol. Methods Quant. Phytoplankt. Anal., 13\u201320."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Grasshoff, K., Kremling, K., and Ehrhardt, M. (1999). Methods of Seawater Analysis, Wiley-VCH. [3rd ed.].","DOI":"10.1002\/9783527613984"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3354\/meps195029","article-title":"Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8column and pyridine-containing mobile phases","volume":"195","author":"Zapata","year":"2000","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_88","unstructured":"R Core Team (2017). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_89","unstructured":"Dinno, A. (2017). Dunn\u2019s Test of Multiple Comparisons Using Rank Sums, The Comprehensive R Archive Network."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"L\u00ea, S., Josse, J., and Husson, F. (2008). FactoMineR: An R Package for Multivariate Analysis. J. Stat. 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