{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:35:21Z","timestamp":1780101321047,"version":"3.54.0"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T00:00:00Z","timestamp":1531353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Screening for compounds that can neutralize the toxicity of tetrodotoxin (TTX) or reduce its negative effects is necessary. Our study tested the TTX detoxification capacity of exopolysaccharide (EPS) extracted from lactic acid bacteria. EPS of Leuconostoc mesenteroides N3 isolated from the Vung Tau sea (Vietnam), Lactobacillus plantarum PN05, and Lactobacillus rhamnosus PN04 were used in the study. To more completely evaluate the importance of EPS in detoxification, EPS samples of Leuconostoc mesenteroides N3, Lactobacillus plantarum PN05 and Lactobacillus rhamnosus PN04 were also tested. The majority of EPS of these bacteria contained glucose; this was observed using thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis. As observed with FTIR analysis, only EPS of Lactobacillus plantarum PN05 contained methyl groups. The results indicated that detoxification of TTX in mice could be obtained at an optimal dose of 248 \u00b5g EPS from Leuconostoc mesenteroides incubated with 54 \u00b5g cuprous oxide for 40 min or 148 \u00b5g EPS Lactobacillus rhamnosus incubated with 55 \u00b5g cuprous oxide for 40 min, while EPS from Lactobacillus plantarum showed TTX detoxification capacity without cuprous oxide combination. Consequently, EPS from Lactobacillus plantarum PN05 can be used in TTX prevention. This is the first report on the importance of lactic acid bacteria in TTX detoxification.<\/jats:p>","DOI":"10.3390\/toxins10070288","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T06:39:59Z","timestamp":1531377599000},"page":"288","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection of the Potential Inactivation of Tetrodotoxin by Lactic Acid Bacterial Exopolysaccharide"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2377-2559","authenticated-orcid":false,"given":"Nguyen Hoang Khue","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Biotechnology, Hochiminh City International University, Vietnam National University-Hochiminh City, Quarter 6, Linh Trung Ward, Thu Duc District, Hochiminh 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nghe Van","family":"Dat","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Biotechnology, Hochiminh City International University, Vietnam National University-Hochiminh City, Quarter 6, Linh Trung Ward, Thu Duc District, Hochiminh 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6247-803X","authenticated-orcid":false,"given":"Le Van","family":"Canh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Biotechnology, Hochiminh City International University, Vietnam National University-Hochiminh City, Quarter 6, Linh Trung Ward, Thu Duc District, Hochiminh 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doan Thi Thanh","family":"Vinh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Biotechnology, Hochiminh City International University, Vietnam National University-Hochiminh City, Quarter 6, Linh Trung Ward, Thu Duc District, Hochiminh 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1080\/14786410903003780","article-title":"Bacterial production of tetrodotoxin in the pufferfish Arothron hispidus","volume":"23","author":"Campbell","year":"2009","journal-title":"Nat. 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