{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:34:54Z","timestamp":1790451294446,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T00:00:00Z","timestamp":1555027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Mycotoxins, the toxic secondary metabolites of mould species, are a growing global concern, rendering almost 25% of all food produced unfit for human or animal consumption, thus placing immense pressure on the food supply chain. Cold Atmospheric pressure Plasma (CAP) represents a promising, low-cost, and environmentally friendly means to degrade mycotoxins with negligible effect on the quality of food products. Despite this promise, the study of CAP-mediated mycotoxin degradation has been limited to a small subset of the vast number of mycotoxins that plague the food supply chain. This study explores the degradation of aflatoxins, trichothecenes, fumonisins, and zearalenone using CAP generated in ambient air. CAP treatment was found to reduce aflatoxins by 93%, trichothecenes by 90%, fumonisins by 93%, and zearalenone by 100% after 8 minutes exposure. To demonstrate the potential of CAP-mediated mycotoxin degradation against more conventional methods, its efficiency was compared against ultraviolet C (UVC) light irradiation. In all cases, CAP was found to be considerably more efficient than UVC, with aflatoxin G1 and zearalenone being completely degraded, levels that could not be achieved using UVC irradiation.<\/jats:p>","DOI":"10.3390\/toxins11040219","type":"journal-article","created":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T12:55:04Z","timestamp":1555073704000},"page":"219","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Mycotoxin Decontamination Efficacy of Atmospheric Pressure Air Plasma"],"prefix":"10.3390","volume":"11","author":[{"given":"Nata\u0161a","family":"Hojnik","sequence":"first","affiliation":[{"name":"Laboratory for Gaseous Electronics F6, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia"},{"name":"Jo\u017eef Stefan International Postgraduate School, Jamova 39, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Modic","sequence":"additional","affiliation":[{"name":"Laboratory for Gaseous Electronics F6, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrijela","family":"Tav\u010dar-Kalcher","sequence":"additional","affiliation":[{"name":"Institute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janja","family":"Babi\u010d","sequence":"additional","affiliation":[{"name":"Institute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, Gerbi\u010deva 60, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Electrical, Engineering and Electronics, University of Liverpool, Liverpool L69 3GJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1957-0789","authenticated-orcid":false,"given":"Uro\u0161","family":"Cvelbar","sequence":"additional","affiliation":[{"name":"Laboratory for Gaseous Electronics F6, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.fct.2013.07.047","article-title":"Mycotoxins: Occurrence, toxicology, and exposure assessment","volume":"60","author":"Marin","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1080\/10408398.2011.571800","article-title":"Food Safety and Increasing Hazard of Mycotoxin Occurrence in Foods and Feeds","volume":"53","author":"Stoev","year":"2013","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.foodcont.2013.08.021","article-title":"Mycotoxins and their effects on human and animal health","volume":"36","author":"Freire","year":"2014","journal-title":"Food Control"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hojnik, N., Cvelbar, U., Tav\u010dar-Kalcher, G., Walsh, J.L., and Kri\u017eaj, I. (2017). Mycotoxin Decontamination of Food: Cold Atmospheric Pressure Plasma versus \u201cClassic\u201d Decontamination. Toxins, 9.","DOI":"10.3390\/toxins9050151"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1007\/s12393-016-9145-3","article-title":"Conventional and Emerging Combination Technologies for Food Processing","volume":"8","author":"Lee","year":"2016","journal-title":"Food Eng. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.tifs.2014.12.001","article-title":"Ultraviolet irradiation detoxification of aflatoxins","volume":"42","author":"Diao","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/jms.1741","article-title":"Photodegradation kinetics and byproducts identification of the Aflatoxin B1 in aqueous medium by ultra-performance liquid chromatography\u2013quadrupole time-of-flight mass spectrometry","volume":"45","author":"Liu","year":"2010","journal-title":"J. Mass Spectrom."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mao, J., He, B., Zhang, L.X., Li, P.W., Zhang, Q., Ding, X.X., and Zhang, W. (2016). A Structure Identification and Toxicity Assessment of the Degradation Products of Aflatoxin B-1 in Peanut Oil under UV Irradiation. Toxins, 8.","DOI":"10.3390\/toxins8110332"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1080\/02652030802057343","article-title":"Reduction of feed-contaminating mycotoxins by ultraviolet irradiation: An in vitro study","volume":"25","author":"Murata","year":"2008","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1146\/annurev-food-022811-101132","article-title":"Cold plasma decontamination of foods","volume":"3","author":"Niemira","year":"2012","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Smet, C., Baka, M., Steen, L., Fraeye, I., Walsh, J.L., Valdramidis, V.P., and Van Impe, J.F. (2018). Combined effect of cold atmospheric plasma, intrinsic and extrinsic factors on the microbial behavior in\/on (food) model systems during storage. Innov. Food Sci. Emerg. Technol.","DOI":"10.1016\/j.ifset.2018.05.016"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.ifset.2016.09.028","article-title":"Influence of food intrinsic factors on the inactivation efficacy of cold atmospheric plasma: Impact of osmotic stress, suboptimal pH and food structure","volume":"38","author":"Smet","year":"2016","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"425201","DOI":"10.1088\/0022-3727\/45\/42\/425201","article-title":"Plasma chemistry model of surface microdischarge in humid air and dynamics of reactive neutral species","volume":"45","author":"Sakiyama","year":"2012","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.ijantimicag.2016.11.022","article-title":"Cold atmospheric pressure plasma elimination of clinically important single-and mixed-species biofilms","volume":"49","author":"Modic","year":"2017","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"355203","DOI":"10.1088\/0022-3727\/49\/35\/355203","article-title":"A solar powered handheld plasma source for microbial decontamination applications","volume":"49","author":"Ni","year":"2016","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6222","DOI":"10.1021\/acs.jafc.7b01604","article-title":"Structures of Degradation Products and Degradation Pathways of Aflatoxin B1 by High-Voltage Atmospheric Cold Plasma (HVACP) Treatment","volume":"65","author":"Shi","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Siciliano, I., Spadaro, D., Prelle, A., Vallauri, D., Cavallero, M.C., Garibaldi, A., and Gullino, M.L. (2016). Use of Cold Atmospheric Plasma to Detoxify Hazelnuts from Aflatoxins. Toxins, 8.","DOI":"10.3390\/toxins8050125"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"ten Bosch, L., Pfohl, K., Avramidis, G., Wieneke, S., Vi\u00f6l, W., and Karlovsky, P. (2017). Plasma-Based Degradation of Mycotoxins Produced by Fusarium, Aspergillus and Alternaria Species. Toxins, 9.","DOI":"10.3390\/toxins9030097"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"203302","DOI":"10.1063\/1.4952574","article-title":"Numerical investigation of the spatiotemporal distribution of chemical species in an atmospheric surface barrier-discharge","volume":"119","author":"Hasan","year":"2016","journal-title":"J. Appl. Phys."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"28499","DOI":"10.1039\/C8CP05762A","article-title":"The generation and transport of reactive nitrogen species from a low temperature atmospheric pressure air plasma source","volume":"20","author":"Dickenson","year":"2018","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_21","first-page":"5","article-title":"Regulation (EC) No 1881\/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs","volume":"364","author":"Commission","year":"2006","journal-title":"Off. J. Eur. Union"},{"key":"ref_22","first-page":"7","article-title":"Recommendation 2006\/576\/EC of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding","volume":"229","author":"Commission","year":"2006","journal-title":"Off. J. Eur. Union"},{"key":"ref_23","first-page":"12","article-title":"Recommendation 2013\/165\/EU of 27 March 2013 on the presence of T-2 and HT-2 toxin in cereals and cereal products","volume":"91","author":"Commission","year":"2013","journal-title":"Off. J. Eur. Union"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.apjtb.2016.07.012","article-title":"Toxicology, biosynthesis, bio-control of aflatoxin and new methods of detection","volume":"6","author":"Gacem","year":"2016","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.anifeedsci.2003.08.008","article-title":"Toxicological evaluation of trichothecenes in animal feed","volume":"114","author":"Eriksen","year":"2004","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3441","DOI":"10.1021\/jf200767q","article-title":"T-2 toxin, a trichothecene mycotoxin: Review of toxicity, metabolism, and analytical methods","volume":"59","author":"Li","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/009841096161447","article-title":"Invited review: Toxicology of deoxynivalenol (vomitoxin)","volume":"48","author":"Rotter","year":"1996","journal-title":"J. Toxicol. Environ. Health Part A"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1111\/1541-4337.12334","article-title":"Exposure, Occurrence, and Chemistry of Fumonisins and their Cryptic Derivatives","volume":"17","author":"Braun","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fct.2006.07.030","article-title":"Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: An oestrogenic mycotoxin","volume":"45","author":"Zinedine","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s12550-013-0178-7","article-title":"UV-induced cis-trans isomerization of zearalenone in contaminated maize","volume":"29","author":"Brezina","year":"2013","journal-title":"Mycotoxin Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.foodcont.2016.09.014","article-title":"Degradation and detoxification of aflatoxin B1 using nitrogen gas plasma generated by a static induction thyristor as a pulsed power supply","volume":"73","author":"Sakudo","year":"2017","journal-title":"Food Control"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1365\/s10337-009-1354-y","article-title":"LC\u2013MS and UPLC\u2013Quadrupole Time-of-Flight MS for Identification of Photodegradation Products of Aflatoxin B1","volume":"71","author":"Liu","year":"2012","journal-title":"Chromatographia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"14003","DOI":"10.1038\/s41598-017-14117-1","article-title":"Directional mass transport in an atmospheric pressure surface barrier discharge","volume":"7","author":"Dickenson","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liu, D.X., Liu, Z.C., Chen, C., Yang, A.J., Li, D., Rong, M.Z., Chen, H.L., and Kong, M.G. (2016). Aqueous reactive species induced by a surface air discharge: Heterogeneous mass transfer and liquid chemistry pathways. Sci. Rep., 6.","DOI":"10.1038\/srep23737"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Liu, Z.C., Liu, D.X., Chen, C., Li, D., Yang, A.J., Rong, M.Z., Chen, H.L., and Kong, M.G. (2015). Physicochemical processes in the indirect interaction between surface air plasma and deionized water. J. Phys. D-Appl. Phys., 48.","DOI":"10.1088\/0022-3727\/48\/49\/495201"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4605","DOI":"10.1021\/cr0206420","article-title":"Atmospheric degradation of volatile organic compounds","volume":"103","author":"Atkinson","year":"2003","journal-title":"Chem. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.physrep.2016.03.003","article-title":"Reactive species in non-equilibrium atmospheric-pressure plasmas: Generation, transport, and biological effects","volume":"630","author":"Lu","year":"2016","journal-title":"Phys. Rep."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/11\/4\/219\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:44:54Z","timestamp":1760186694000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/11\/4\/219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,12]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4]]}},"alternative-id":["toxins11040219"],"URL":"https:\/\/doi.org\/10.3390\/toxins11040219","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,12]]}}}