{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:46:05Z","timestamp":1790649965486,"version":"4.1.0"},"reference-count":78,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Hessen State Ministry of Higher Education, Research and the Arts (HMWK) via the \u201cLOEWE Center for Insect Biotechnology and Bioresources\u201d","award":["the Hessen State Ministry of Higher Education, Research and the Arts (HMWK) via the \u201cLOEWE Center for Insect Biotechnology and Bioresources\u201d"],"award-info":[{"award-number":["the Hessen State Ministry of Higher Education, Research and the Arts (HMWK) via the \u201cLOEWE Center for Insect Biotechnology and Bioresources\u201d"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Ant venoms contain many small, linear peptides, an untapped source of bioactive peptide toxins. The control of agricultural insect pests currently depends primarily on chemical insecticides, but their intensive use damages the environment and human health, and encourages the emergence of resistant pest populations. This has promoted interest in animal venoms as a source of alternative, environmentally-friendly bio-insecticides. We tested the crude venom of the predatory ant, Manica rubida, and observed severe fitness costs in the parthenogenetic pea aphid (Acyrthosiphon pisum), a common agricultural pest. Therefore, we explored the M. rubida venom peptidome and identified a novel decapeptide U-MYRTX-MANr1 (NH2-IDPKVLESLV-CONH2) using a combination of Edman degradation and de novo peptide sequencing. Although this myrmicitoxin was inactive against bacteria and fungi, it reduced aphid survival and reproduction. Furthermore, both crude venom and U-MYRTX-MANr1 reversibly paralyzed injected aphids and induced a loss of body fluids. Components of M. rubida venom may act on various biological targets including ion channels and hemolymph coagulation proteins, as previously shown for other ant venom toxins. The remarkable insecticidal activity of M. rubida venom suggests it may be a promising source of additional bio-insecticide leads.<\/jats:p>","DOI":"10.3390\/toxins11100562","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T03:06:51Z","timestamp":1569467211000},"page":"562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Identification and Functional Characterization of a Novel Insecticidal Decapeptide from the Myrmicine Ant Manica rubida"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4412-4748","authenticated-orcid":false,"given":"John","family":"Heep","sequence":"first","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6849-2596","authenticated-orcid":false,"given":"Marisa","family":"Skaljac","sequence":"additional","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Grotmann","sequence":"additional","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Kessel","sequence":"additional","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Seip","sequence":"additional","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrike","family":"Schmidtberg","sequence":"additional","affiliation":[{"name":"Institute for Insect Biotechnology, Justus Liebig University of Giessen, Heinrich-Buff- Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Vilcinskas","sequence":"additional","affiliation":[{"name":"Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Winchesterstrasse 2, 35394 Giessen, Germany"},{"name":"Institute for Insect Biotechnology, Justus Liebig University of Giessen, Heinrich-Buff- Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,25]]},"reference":[{"key":"ref_1","unstructured":"(2019, July 01). 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