{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:29:06Z","timestamp":1768476546205,"version":"3.49.0"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The sensitivity of vertebrate citrated plasma to pro- and anticoagulant venom or toxins occurs on a microscale level (micrograms). Although it improves responses to agonists, recalcification triggers a relatively fast thrombin formation process in mammalian plasma. As it has a natural factor XII deficiency, the recalcification time (RT) of chicken plasma (CP) is comparatively long [\u2265 1800 seconds (s)]. Our objective was to compare the ability of bee venom phospholipase A2 (bvPLA2) to neutralize clot formation induced by an activator of coagulation (the aPTT clot) in recalcified human and chicken plasmas, through rotational thromboelastometry. The strategy used in this study was to find doses of bvPLA2 that were sufficient enough to prolong the clotting time (CT) of these activated plasmas to values within their normal RT range. The CT of CP was prolonged in a dose-dependent manner by bvPLA2, with 17 \u00b1 2.8 ng (n = 6) being sufficient to displace the CT values of the activated samples to \u2265 1800 s. Only amounts up to 380 \u00b1 41 ng (n = 6) of bvPLA2 induced the same effect in activated human plasma samples. In conclusion, the high sensitivity of CP to agonists and rotational thromboelastometry could be useful. For example, during screening procedures for assaying the effects of toxins in several stages of the coagulation pathway, such as clot initiation, formation, stability, strength, or dissolution.<\/jats:p>","DOI":"10.3390\/toxins12020079","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T07:41:11Z","timestamp":1580110871000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Factor XII-Deficient Chicken Plasma as a Useful Target for Screening of Pro- and Anticoagulant Animal Venom Toxins"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5779-3668","authenticated-orcid":false,"given":"Benedito C.","family":"Prezoto","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology, The Butantan Institute, Av. Dr. Vital Brazil 1500, S\u00e3o Paulo CEP 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Oguiura","sequence":"additional","affiliation":[{"name":"Ecology and Evolution Laboratory, The Butantan Institute, Av. Dr. Vital Brazil 1500, S\u00e3o Paulo CEP 05503-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1046\/j.1440-1681.2002.03725.x","article-title":"Molecular moulds with multiple missions: Functional sites in three-finger toxins","volume":"29","author":"Kini","year":"2002","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_2","first-page":"86","article-title":"Workshop overview: Scientific and regulatory challenges for the reduction, refinement, and replacement of animals in toxicity testing","volume":"43","author":"Purchase","year":"1998","journal-title":"Toxicol. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/0041-0101(89)90096-2","article-title":"Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom","volume":"27","author":"Gene","year":"1989","journal-title":"Toxicon"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/0041-0101(92)90438-B","article-title":"Comparison of the potency of three Brazilian Bothrops antivenoms using in vivo rodent and in vitro assays. BIASG (Butantan Institute Antivenom Study Group)","volume":"30","author":"Laing","year":"1992","journal-title":"Toxicon"},{"key":"ref_5","first-page":"143","article-title":"Effects of antithrombin III and antivenom on procoagulant activity of Russell\u2019s viper venom in a whole blood model","volume":"26","author":"Clemens","year":"1995","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1177\/0310057X9602400314","article-title":"Efficacy and potency of antivenoms in neutralizing the procoagulant effects of Australian snake venoms in dog and human plasma","volume":"24","author":"Sprivulis","year":"1996","journal-title":"Anaesth. Intensive Care"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0041-0101(03)00125-9","article-title":"Animal experimentation in snake venom research and in vitro alternatives","volume":"42","author":"Sells","year":"2003","journal-title":"Toxicon"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.toxicon.2006.09.007","article-title":"ASP Investigators. Efficacy of antivenom against the procoagulant effect of Australian brown snake (Pseudonaja sp.) venom: In vivo and in vitro studies","volume":"49","author":"Isbister","year":"2007","journal-title":"Toxicon"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.toxicon.2010.03.013","article-title":"Endogenous thrombin potential as a novel method for the characterization of procoagulant snake venoms and the efficacy of antivenom","volume":"56","author":"Isbister","year":"2010","journal-title":"Toxicon"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.toxicon.2009.08.010","article-title":"Preclinical assessment of the efficacy of a new antivenom (EchiTAb-Plus-ICP) for the treatment of viper envenoming in sub-Saharan Africa","volume":"55","author":"Segura","year":"2010","journal-title":"Toxicon"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jprot.2014.06.016","article-title":"Preclinical efficacy of Australian antivenoms against the venom of the small-eyed snake, Micropechis ikaheka, from Papua New Guinea: An antivenomics and neutralization study","volume":"110","author":"Pla","year":"2014","journal-title":"J. Proteom."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jprot.2016.09.007","article-title":"Proteomics and antivenomics of Papuan black snake (Pseudechis papuanus) venom with analysis of its toxicological profile and the preclinical efficacy of Australian antivenoms","volume":"150","author":"Pla","year":"2017","journal-title":"J. Proteom."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2013.10.021","article-title":"Development of an in vitro potency assay for antivenom against Malayan pit viper (Calloselasma rhodostoma)","volume":"77","author":"Pornmuttakun","year":"2014","journal-title":"Toxicon"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.toxicon.2015.09.027","article-title":"Evaluation of the preclinical efficacy of four antivenoms, distributed in sub-Saharan Africa, to neutralize the venom of the carpet viper, Echis ocellatus, from Mali, Cameroon, and Nigeria","volume":"106","author":"Sanchez","year":"2015","journal-title":"Toxicon"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.toxicon.2016.06.022","article-title":"Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics","volume":"119","author":"Calvete","year":"2016","journal-title":"Toxicon"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1055\/s-0029-1214152","article-title":"Procoagulant snake toxins: Laboratory studies, diagnosis, and understanding snakebite coagulopathy","volume":"35","author":"Isbister","year":"2009","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_17","first-page":"949","article-title":"Development of simple assay standard procedures for the characterization of snake venoms","volume":"61","author":"Theakston","year":"1983","journal-title":"Bull. World Health Organ."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vascn.2009.06.004","article-title":"A turbidimetric assay for the measurement of clotting times of procoagulant venoms in plasma","volume":"61","author":"Isbister","year":"2010","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1146\/annurev.bi.57.070188.004411","article-title":"Cofactor proteins in the assembly and expression of blood clotting enzyme complexes","volume":"57","author":"Mann","year":"1988","journal-title":"Annu. Rev. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1016\/j.bbamcr.2017.07.009","article-title":"The plasma contact system, a protease cascade at the nexus of inflammation, coagulation and immunity","volume":"864","author":"Weidmann","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1172\/JCI103109","article-title":"A familial hemorrhagic trait associated with a deficiency of a clot-promoting fraction of plasma","volume":"34","author":"Ratnoff","year":"1955","journal-title":"J. Clin. Investig."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1111\/j.1538-7836.2008.03143.x","article-title":"Evolution of the contact phase of vertebrate blood coagulation","volume":"6","author":"Ponczek","year":"2008","journal-title":"J. Thromb. Haemost."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/ajh.23599","article-title":"TEG and ROTEM: Technology and clinical applications","volume":"89","author":"Whiting","year":"2014","journal-title":"Am. J. Hematol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1016\/0041-0101(94)90320-4","article-title":"Action of Calloselasma rhodostoma (Malayan pit viper) venom on human blood coagulation and fibrinolysis using computerized thromboelastography (CTEG)","volume":"32","author":"Dambisya","year":"1994","journal-title":"Toxicon"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1016\/0041-0101(95)00067-V","article-title":"Anticoagulant effects of Pseudechis australis (Australian king brown snake) venom on human blood: A computerized thromboelastography study","volume":"33","author":"Dambisya","year":"1995","journal-title":"Toxicon"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1111\/vec.12236","article-title":"Hemostatic analysis of dogs naturally envenomed by the African puffadder (Bitis arietans) and snouted cobra (Naja annulifera)","volume":"24","author":"Nagel","year":"2014","journal-title":"J. Vet. Emerg. Crit. Care (San Antonio)"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.toxicon.2015.11.014","article-title":"Differential action of medically important Indian BIG FOUR snake venoms on rodent blood coagulation","volume":"110","author":"Hiremath","year":"2016","journal-title":"Toxicon"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1097\/MBC.0000000000000577","article-title":"Thrombelastographic characterization of the thrombin-like activity of Crotalus simus and Bothrops asper venoms","volume":"28","author":"Nielsen","year":"2017","journal-title":"Blood Coagul. Fibrinolysis"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/bcpt.12848","article-title":"Characterization of the rabbit as an in vitro and in vivo model to assess the effects of fibrinogenolytic activity of snake venom on coagulation","volume":"122","author":"Nielsen","year":"2018","journal-title":"Basic Clin. Pharmacol. Toxicol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.toxicon.2014.08.004","article-title":"An alternative micromethod to access the procoagulant activity of Bothrops jararaca venom and the efficacy of antivenom","volume":"90","author":"Oguiura","year":"2014","journal-title":"Toxicon"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.toxicon.2018.04.009","article-title":"A functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against Crotalus durissus terrificus venom","volume":"148","author":"Prezoto","year":"2018","journal-title":"Toxicon"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s00239-003-2461-2","article-title":"Molecular evolution and phylogeny of elapid snake venom three-finger toxins","volume":"57","author":"Fry","year":"2003","journal-title":"J. Mol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lynch, V.J. (2007). Inventing an arsenal: Adaptive evolution and neofunctionalization of snake venom phospholipase A2 genes. BMC Evol. Biol., 7.","DOI":"10.1186\/1471-2148-7-2"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.tree.2012.10.020","article-title":"Complex cocktails: The evolutionary novelty of venoms","volume":"28","author":"Casewell","year":"2013","journal-title":"Trends Ecol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1016\/0041-0101(95)00103-4","article-title":"The role of enzymatic activity in inhibition of the extrinsic tenase complex by phospholipase A2 isoenzymes from Naja nigricollis venom","volume":"33","author":"Kini","year":"1995","journal-title":"Toxicon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1006\/abbi.1999.1345","article-title":"Targeting of Venom Phospholipases: The strongly anticoagulant phospholipase A2 from Naja nigricollis venom binds to coagulation Factor xa to inhibit the prothrombinase complex","volume":"369","author":"Kerns","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.toxicon.2005.02.018","article-title":"Structure\u2014Function relationships and mechanism of anticoagulant phospholipase A2 enzymes from snake venoms","volume":"45","author":"Kini","year":"2005","journal-title":"Toxicon"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0041-0101(79)90305-2","article-title":"Anticoagulant properties of Apis mellifera (honey bee) venom","volume":"17","author":"Ouyang","year":"1979","journal-title":"Toxicon"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0041-0101(96)00078-5","article-title":"Melittin and phospholipase A2 from bee (Apis mellifera) venom cause necrosis of murine skeletal muscle in vivo","volume":"35","author":"Ownby","year":"1997","journal-title":"Toxicon"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.toxicon.2010.07.005","article-title":"Acute physiological effects of honeybee (Apis mellifera) envenoming by subcutaneousroute in a mouse model","volume":"56","author":"Prado","year":"2010","journal-title":"Toxicon"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s11239-019-01980-0","article-title":"The anticoagulant effect of Apis mellifera phospholipase A2 is inhibited by CORM-2 via a carbon monoxide-independent mechanism","volume":"49","author":"Nielsen","year":"2020","journal-title":"J. Thromb. Thrombolysis"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/bja\/77.2.157","article-title":"Thromboelastography changes in preeclampsia and eclampsia","volume":"77","author":"Orlikowski","year":"1996","journal-title":"Br. J. Anaesth."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1111\/j.1537-2995.2009.02540.x","article-title":"Therapeutic efficacy of platelet transfusion in patients with acute leukemia: An evaluation of methods","volume":"50","author":"Apelseth","year":"2010","journal-title":"Transfusion"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1053\/j.jvca.2009.07.012","article-title":"In vitro comparative study of hemostatic components in warfarin-treated and fibrinogen-deficient plasma","volume":"24","author":"Rumph","year":"2010","journal-title":"J. Cardiothorac. Vasc. Anesth."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1161\/CIRCRESAHA.110.233056","article-title":"Microparticles in hemostasis and thrombosis","volume":"108","author":"Owens","year":"2011","journal-title":"Circ. Res."},{"key":"ref_46","first-page":"2420","article-title":"Membrane microparticles and diseases","volume":"17","author":"Wu","year":"2013","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_47","unstructured":"Brown, E.B., and Moore, C.V. (1966). The Biology and Pathology of the Initial Stages of Blood Coagulation, Heinemann."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/0305-0491(85)90502-4","article-title":"Coagulation of plasma from the chicken (Gallus domesticus): Phospholipids influence clotting rates induced by components from Russell\u2019s viper venom","volume":"82","author":"Johnson","year":"1985","journal-title":"Comp. Biochem. Physiol. B"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bernardoni, J.L., Sousa, L.F., Wermelinger, L.S., Lopes, A.S., Prezoto, B.C., Serrano, S.M., Zingali, R.B., and Moura-da-Silva, A.M. (2014). Functional variability of snake venom metalloproteinases: Adaptive advantages in targeting different prey and implications for human envenomation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0109651"}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/2\/79\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:30:23Z","timestamp":1760362223000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/2\/79"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,23]]},"references-count":49,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["toxins12020079"],"URL":"https:\/\/doi.org\/10.3390\/toxins12020079","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,23]]}}}