{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:26:49Z","timestamp":1790778409748,"version":"4.1.0"},"reference-count":153,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Bacillus thuringiensis (Bt) is a ubiquitous bacterium in soils, insect cadavers, phylloplane, water, and stored grain, that produces several proteins, each one toxic to different biological targets such as insects, nematodes, mites, protozoa, and mammalian cells. Most Bt toxins identify their particular target through the recognition of specific cell membrane receptors. Cry proteins are the best-known toxins from Bt and a great amount of research has been published. Cry are cytotoxic to insect larvae that affect important crops recognizing specific cell membrane receptors such as cadherin, aminopeptidase-N, and alkaline phosphatase. Furthermore, some Cry toxins such as Cry4A, Cry4B, and Cry11A act synergistically with Cyt toxins against dipteran larvae vectors of human disease. Research developed with Cry proteins revealed that these toxins also could kill human cancer cells through the interaction with specific receptors. Parasporins are a small group of patented toxins that may or may not have insecticidal activity. These proteins could kill a wide variety of mammalian cancer cells by recognizing specific membrane receptors, just like Cry toxins do. Surface layer proteins (SLP), unlike the other proteins produced by Bt, are also produced by most bacteria and archaebacteria. It was recently demonstrated that SLP produced by Bt could interact with membrane receptors of insect and human cancer cells to kill them. Cyt toxins have a structure that is mostly unrelated to Cry toxins; thereby, other mechanisms of action have been reported to them. These toxins affect mainly mosquitoes that are vectors of human diseases like Anopheles spp (malaria), Aedes spp (dengue, zika, and chikungunya), and Culex spp (Nile fever and Rift Valley fever), respectively. In addition to the Cry, Cyt, and parasporins toxins produced during spore formation as inclusion bodies, Bt strains also produce Vip (Vegetative insecticidal toxins) and Sip (Secreted insecticidal proteins) toxins with insecticidal activity during their vegetative growth phase.<\/jats:p>","DOI":"10.3390\/toxins12050301","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T04:46:07Z","timestamp":1588826767000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["The Cytocidal Spectrum of Bacillus thuringiensis Toxins: From Insects to Human Cancer Cells"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0426-7410","authenticated-orcid":false,"given":"Gretel","family":"Mendoza-Almanza","sequence":"first","affiliation":[{"name":"National Council of Science and Technology, Autonomous University of Zacatecas, Zacatecas 98000, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar L.","family":"Esparza-Ibarra","sequence":"additional","affiliation":[{"name":"Academic Unit of Biological Sciences, Autonomous University of Zacatecas, Zacatecas 98068, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge L.","family":"Ayala-Luj\u00e1n","sequence":"additional","affiliation":[{"name":"Academic Unit of Chemical Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Mercado-Reyes","sequence":"additional","affiliation":[{"name":"Academic Unit of Biological Sciences, Autonomous University of Zacatecas, Zacatecas 98068, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0340-9381","authenticated-orcid":false,"given":"Susana","family":"Godina-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Academic Unit of Chemical Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Hern\u00e1ndez-Barrales","sequence":"additional","affiliation":[{"name":"Academic Unit of Chemical Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Zacatecas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Olmos-Soto","sequence":"additional","affiliation":[{"name":"Department of Marine Biotechnology, Center for Scientific Research and Higher Education of Ensenada. Ensenada 22860, Baja California, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/bies.950150706","article-title":"The crystal delta-endotoxins of Bacillus thuringiensis\u2014Models for their mechanism of action on the insect gut","volume":"15","author":"Knowles","year":"1993","journal-title":"Bioessays"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/MMBR.62.3.775-806.1998","article-title":"Bacillus thuringiensis and its pesticidal crystal proteins","volume":"62","author":"Schnepf","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.toxicon.2006.11.022","article-title":"Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control","volume":"49","author":"Bravo","year":"2007","journal-title":"Toxicon"},{"key":"ref_4","first-page":"547","article-title":"Bacillus thuringiensis as a specific, safe, and effective tool for insect pest control","volume":"17","author":"Roh","year":"2007","journal-title":"J. Mol. Biol."},{"key":"ref_5","unstructured":"Charles, J.F., Del\u00e9cluse, A., and Nielsen-LeRoux, C. (2000). Bacterial control of vector-mosquitoes and black flies. Entomopathogenic Bacteria: From Laboratory to Field Application, Kluwer Academic Publishers."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.ijpara.2005.03.010","article-title":"Toxicity of Bacillus Thuringiensis to Parasitic and Free-Living Life-Stages of Nematode Parasites of Livestock","volume":"35","author":"Kotze","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_7","first-page":"427","article-title":"Parasporin, a new anticancer protein group from Bacillus thuringiensis","volume":"29","author":"Ohba","year":"2009","journal-title":"Anticancer Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4014\/jmb.1607.07020","article-title":"Identification of a Bacillus thuringiensis Surface Layer Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells","volume":"27","author":"Rubio","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2008.01087.x","article-title":"A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis","volume":"282","author":"Gang","year":"2008","journal-title":"FEMS. Microbiol. Lett."},{"key":"ref_10","first-page":"201","article-title":"The Defined Toxin-binding Region of the Cadherin G-protein Coupled Receptor, BT-R1, for the Active Cry1Ab Toxin of Bacillus thuringiensis","volume":"11","author":"Liu","year":"2018","journal-title":"J. Proteomics Bioinform."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.3390\/toxins6123296","article-title":"Bacillus thuringiensis toxins: An overview of their biocidal activity","volume":"6","author":"Palma","year":"2014","journal-title":"Toxins"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3000","DOI":"10.1016\/S0021-9258(19)69714-0","article-title":"Purification and characterization of the entomocidal protoxin of Bacillus thuringiensis","volume":"256","author":"Bulla","year":"1981","journal-title":"J. Biol. Chem."},{"key":"ref_13","unstructured":"Crickmore, N., Zeigler, D.R., Schnepf, E., Rie, J., Lereclus, D., Baum, J., Bravo, A., and Dean, D.H. (2020, April 03). Bacillus thuringiensis Toxin Nomenclature. Available online: http:\/\/www.lifesci.sussex.ac.uk\/home\/Neil_Crickmore\/Bt\/."},{"key":"ref_14","first-page":"242","article-title":"Insecticidal crystal proteins of Bacillus thuringiensis","volume":"5","author":"Whiteley","year":"1989","journal-title":"Microbiol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jip.2009.02.009","article-title":"Insecticidal activity of Bacillus thuringiensis crystal proteins","volume":"101","year":"2009","journal-title":"J. Invertebr. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jip.2016.10.005","article-title":"Parasporins 1 and 2: Their structure and activity","volume":"142","author":"Akiba","year":"2016","journal-title":"J. Invertebr. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1128\/JB.182.4.859-868.2000","article-title":"S-Layer Proteins","volume":"182","author":"Sleytr","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/AEM.72.1.353-360.2006","article-title":"A Bacillus thuringiensis S-layer protein involved in toxicity against Epilachna varivestis (Coleoptera: Coccinellidae)","volume":"72","author":"Bravo","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/s00253-006-0332-7","article-title":"Discovery and characterization of Sip1A: A novel secreted protein from Bacillus thuringiensis with activity against coleopteran larvae","volume":"72","author":"Donovan","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1128\/MMBR.00060-15","article-title":"Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria","volume":"80","author":"Chakroun","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sahin, B., Gomis-Cebolla, J., G\u00fcnes\u0327, H., and Ferr\u00e9, J. (2018). Characterization of Bacillus thuringiensis isolates by their insecticidal activity and their production of Cry and Vip3 proteins. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0206813"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.tplants.2006.04.001","article-title":"Recent developments and future prospects in insect pest control in transgenic crop","volume":"11","author":"Christou","year":"2006","journal-title":"Trend Plant Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1574-6976.2012.00341.x","article-title":"Bacillus thuringiensis insecticidal three-domain Cry toxins: Mode of action, insect resistance and consequences for crop protection","volume":"37","author":"Soberon","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1038\/nbt0903-1003","article-title":"Are Bt crops safe?","volume":"21","author":"Mendelson","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/nbt1180","article-title":"Transgenic crops expressing Bacillus thuringiensis toxins and biological control","volume":"24","author":"Romeis","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chen, M., Zhao, J.Z., Collins, H.L., Earle, E.D., Cao, J., and Shelton, A.M. (2008). A critical assessment of the effects of Bt transgenic pants on parasitoids. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002284"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3109\/07388551.2014.960793","article-title":"Bacillus thuringiensis: Mechanism of action, resistance, and new applications: A review","volume":"36","author":"Melo","year":"2016","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1038\/nbt.3974","article-title":"Surge in insect resistance to transgenic crops and prospects for sustainability","volume":"35","author":"Tabashnik","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1038\/nbt0887-807","article-title":"Insect tolerant transgenic tomato plants","volume":"5","author":"Fischhoff","year":"1987","journal-title":"Nat. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/328033a0","article-title":"Transgenic plants protected from insects","volume":"325","author":"Vaek","year":"1987","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3389\/fpls.2015.00283","article-title":"The food and environmental safety of Bt crops","volume":"6","author":"Koch","year":"2015","journal-title":"Front. Plant. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"b131","DOI":"10.1006\/jipa.2000.4962","article-title":"Characterization of a Bacillus thuringiensis delta endotoxin whic iIs toxic to insects in three orders","volume":"76","author":"Zhong","year":"2000","journal-title":"J. Invertebr. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1073\/pnas.78.5.2893","article-title":"Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli","volume":"78","author":"Schnepf","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1128\/AEM.66.1.118-124.2000","article-title":"Plasmid Transfer between the Bacillus thuringiensis Subspecies kurstaki and tenebrionis in Laboratory Culture and Soil and in Lepidopteran and Coleopteran Larvae","volume":"66","author":"Thomas","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12497","DOI":"10.1074\/jbc.M109.085266","article-title":"Role of alkaline phosphatase from Manduca sexta in the mechanism of action of Bacillus thuringiensis Cry1Ab toxin","volume":"285","author":"Arenas","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.pbiomolbio.2004.01.009","article-title":"Pore forming protein toxins: From structure to function","volume":"88","author":"Parker","year":"2005","journal-title":"Progress. Biophys. Mol. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Almaz\u00e1n, C., Zavala, L.E., Mu\u00f1oz-Garay, C., Jim\u00e9nez-Ju\u00e1rez, N., Pacheco, S., Masson, L., Sober\u00f3n, M., and Bravo, A. (2009). Dominant Negative Mutants of Bacillus thuringiensis Cry1Ab Toxin Function as Anti-Toxins: Demonstration of the Role of Oligomerization in Toxicity. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0005545"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0168-9525(01)02237-5","article-title":"How Bacillus thuringiensis has evolved specific toxins to colonize the insect world","volume":"17","author":"Bravo","year":"2001","journal-title":"Trends Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"25220","DOI":"10.1074\/jbc.271.41.25220","article-title":"Mutations at domain II, loop 3, of Bacillus thuringiensis CryIAa and CryIAb \u03b4-endotoxins suggest loop 3 is involved in initial binding to lepidopteran midguts","volume":"271","author":"Rajamohan","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1046\/j.1365-2958.2000.02109.x","article-title":"Role of two arginine residues in domain II, loop 2 of Cry1Ab and Cry1Ac Bacillus thuringiensis \u03b4-endotoxin in toxicity and binding to Manduca sexta and Lymantria dispar aminopeptidase N","volume":"38","author":"Lee","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.genet.37.110801.143042","article-title":"Structure, diversity, and evolution of protein toxins from spore-forming entomopathogenic bacteria","volume":"37","author":"Bravo","year":"2003","journal-title":"Annu. Rev. Genet."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0300-483X(94)90156-2","article-title":"All in the family: The toxic activity of pore-forming toxins","volume":"87","author":"Lakey","year":"1994","journal-title":"Toxicology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/978-1-4419-6327-7_10","article-title":"Hemolysin E (HlyE, ClyA, SheA) and related toxins","volume":"677","author":"Hunt","year":"2010","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.toxicon.2009.02.026","article-title":"Molecular mechanism of pore formation by actinoporins","volume":"54","author":"Kristan","year":"2009","journal-title":"Toxicon"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tim.2013.10.004","article-title":"Cell targeting by the Staphylococcus aureus pore-forming toxins: It\u2019s not just about lipids","volume":"22","author":"DuMont","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_46","unstructured":"Iacovache, I., Dal Peraro, M., and van der Goot, F.G. (2015). The Comprehensive Sourcebook of Bacterial Protein Toxins, Elsevier Ltd."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1128\/IAI.00927-12","article-title":"Identification and characterization of the first cholesterol-dependent cytolysins from Gram-negative bacteria","volume":"81","author":"Hotze","year":"2013","journal-title":"Infect. Immun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/S0959-440X(97)80123-6","article-title":"Channel-forming toxins: Tales of transformation","volume":"7","author":"Gouaux","year":"1997","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3109\/09687689709068435","article-title":"Membrane insertion: The strategy of toxins","volume":"14","author":"Lesieur","year":"1997","journal-title":"Mol. Membrane Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.sbi.2010.01.013","article-title":"Structure and assembly of pore-forming proteins","volume":"20","author":"Iacovache","year":"2010","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/nrmicro.2015.3","article-title":"Pore-forming toxins: Ancient, but never really out of fashion","volume":"14","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"14563","DOI":"10.1021\/bi981452f","article-title":"Identification of a membrane- spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O: An \u03b1-helical to \u03b2-sheet transition identified by fluorescence spectroscopy","volume":"37","author":"Shepard","year":"1998","journal-title":"Biochemistry"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.3390\/toxins6092732","article-title":"Structural Insights into Bacillus thuringiensis Cry, Cyt and Parasporin Toxins","volume":"6","author":"Xu","year":"2014","journal-title":"Toxins"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"13863","DOI":"10.1074\/jbc.M110057200","article-title":"Heliothis virescens and Manduca sexta lipid rafts are involved in Cry1A toxin binding to the midgut epithelium and subsequent pore formation","volume":"277","author":"Zhuang","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.bbamem.2004.08.013","article-title":"Oligomerization triggers binding of a Bacillus thuringiensis Cry1Ab pore-forming toxin to aminopeptidase N receptor leading to insertion into membrane microdomains","volume":"1667","author":"Bravo","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Bravo, A., Sober\u00f3n, M., and Gill, S.S. (2005). Bacillus thuringiensis Mechanisms and Use. Compr. Mol. Insect Sci., 175\u2013205.","DOI":"10.1016\/B0-44-451924-6\/00081-8"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"10329","DOI":"10.1021\/bi060297z","article-title":"Structural changes of the Cry1Ac oligomeric pre-pore from Bacillus thuringiensis induced by N-acetylgalactosamine facilitates toxin membrane insertion","volume":"45","author":"Rausell","year":"2006","journal-title":"Biochemistry"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1128\/MMBR.00034-06","article-title":"Role of receptors in Bacillus thuringiensis crystal toxin activity","volume":"71","author":"Pigott","year":"2007","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/B978-0-12-800197-4.00002-6","article-title":"Diversity of Bacillus thuringiensis crystal toxins and mechanism of action","volume":"47","author":"Adang","year":"2014","journal-title":"Adv. Insect Physiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"28906","DOI":"10.1074\/jbc.M103007200","article-title":"Mapping the epitope in cadherin-like receptors involved in Bacillus thuringiensis Cry1A toxin interaction using phage display","volume":"276","author":"Oltean","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"30137","DOI":"10.1074\/jbc.M203121200","article-title":"Hydropathic complementarity determines interaction of epitope (869) HITDTNNK (876) in Manduca sexta Bt-R(1) receptor with loop 2 of domain II of Bacillus thuringiensis Cry1A toxins","volume":"277","author":"Gomez","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"10482","DOI":"10.1021\/bi034440p","article-title":"Molecular basis for Bacillus thuringiensis Cry1Ab toxin specificity: Two structural determinants in the Manduca sexta Bt-R1 receptor interact with loops \u03b18 and 2 in domain II of Cy1Ab toxin","volume":"42","author":"Dean","year":"2003","journal-title":"Biochemistry"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/S0965-1748(02)00040-1","article-title":"Cry1A toxins of Bacillus thuringiensis bind specifically to a region adjacent to the membrane-proximal extracellular domain of BT-R1 in Manduca sexta: Involvement of a cadherin in the entomopathogenicity of Bacillus thuringiensis","volume":"32","author":"Dorsch","year":"2002","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/S0014-5793(02)02321-9","article-title":"Cadherin-like receptor binding facilitates proteolytic cleavage of helix alpha-1 in domain I and oligomer pre-pore formation of Bacillus thuringiensis Cry1Ab toxin","volume":"513","author":"Miranda","year":"2002","journal-title":"FEBS Lett."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1007\/s00284-012-0178-4","article-title":"Investigation of the Cry4B-prohibitin interaction in Aedes aegypti cells","volume":"65","author":"Kuadkitkan","year":"2012","journal-title":"Curr. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1006\/jmbi.1995.0630","article-title":"Bacillus thuringiensis CrylA(a) insecticidal toxin: Crystal structure and channel formation","volume":"254","author":"Grochulski","year":"1995","journal-title":"J. Mol. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"32750","DOI":"10.1074\/jbc.M109.024968","article-title":"Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a \u201cping pong\u201d binding mechanism with Manduca sexta aminopeptidase-N and cadherin receptors","volume":"284","author":"Pacheco","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1038\/s41598-017-02545-y","article-title":"ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth","volume":"7","author":"Ocelotl","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.pestbp.2012.05.007","article-title":"Learning the ABCs of Bt: ABC transporters and insect resistance to Bacillus thuringiensis provide clues to a crucial step in toxin mode of action","volume":"104","author":"Heckel","year":"2012","journal-title":"Pest. Biochem. Physiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1126\/science.1146453","article-title":"Engineering modified Bt toxins to counter insect resistance","volume":"318","author":"Lopez","year":"2007","journal-title":"Science"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.ibmb.2011.04.013","article-title":"Tobacco plants expressing the Cry1AbMod toxin suppress tolerance to Cry1Ab toxin of Manduca sexta cadherin-silenced larvae","volume":"41","author":"Porta","year":"2011","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1038\/nbt.1988","article-title":"Efficacy of genetically modified Bt toxins against insects with different mechanisms of resistance","volume":"29","author":"Tabashnik","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1021\/bp00017a003","article-title":"Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system","volume":"8","author":"Wickham","year":"1992","journal-title":"Biotechnol. Prog."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Castella, C., Pauron, D., Hilliou, F., Trang, V.T., Zucchini-Pascal, N., Gallet, A., and Barbero, P. (2019). Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity. Biol. Open, 18.","DOI":"10.1242\/bio.037085"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1006\/jipa.1997.4723","article-title":"Toxicity and binding properties of the Bacillus thuringiensis delta-endotoxin Cry1C to cultured insect cells","volume":"71","author":"Kwa","year":"1998","journal-title":"J. Invertebr. Pathol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"9897","DOI":"10.1073\/pnas.0604017103","article-title":"A mechanism of cell death involving an adenylyl cyclase\/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis","volume":"103","author":"Zhang","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_77","first-page":"129","article-title":"Structure of the mosquitocidal delta-endotoxin CytB from Bacillus thuringiensis ssp","volume":"257","author":"Li","year":"1996","journal-title":"kyushuensis and implications for membrane pore formation. J. Mol. Biol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1111\/1744-7917.12401","article-title":"Effects and mechanisms of Bacillus thuringiensis crystal toxins for mosquito larvae","volume":"24","author":"Zhang","year":"2017","journal-title":"Insect Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"5082","DOI":"10.1128\/AEM.68.10.5082-5095.2002","article-title":"Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis","volume":"68","author":"Berry","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1128\/AEM.69.5.2415-2422.2003","article-title":"Cytolytic toxin Cyt1A and its mechanism of membrane damage: Data and hypotheses","volume":"69","author":"Butko","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1242\/jcs.60.1.181","article-title":"Bacillus thuringiensis var. israeliensis crystal delta-endotoxin: Effects on insect and mammalian cells in vitro and in vivo","volume":"60","author":"Thomas","year":"1983","journal-title":"J. Cell Sci."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"8465","DOI":"10.1073\/pnas.1222144110","article-title":"Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests","volume":"110","author":"Chogule","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.jmb.2004.08.045","article-title":"Crystal structures and electron micrographs of fungal volvatoxin A2","volume":"343","author":"Lin","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/246524a0","article-title":"Isolation of a new cardiotoxic protein from the edible mushroom, Volvariella volvacea","volume":"246","author":"Lin","year":"1973","journal-title":"Nature"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1099\/mic.0.28981-0","article-title":"Cyt1Ca from Bacillus thuringiensis subsp. israelensis: Production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins","volume":"152","author":"Manasherob","year":"2006","journal-title":"Microbiology"},{"key":"ref_86","unstructured":"Alouf, J.E. (2015). Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests. Comprehensive Sourcebook of Bacterial Protein Toxins, Elsevier. [4th ed.]."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"11355","DOI":"10.1021\/bi960970s","article-title":"Membrane permeabilization induced by cytolytic delta-endotoxin CytA from Bacillus thuringiensis var. israelensis","volume":"35","author":"Butko","year":"1996","journal-title":"Biochemistry"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1128\/AEM.67.7.3280-3284.2001","article-title":"Cyt1Ab1 and Cyt2Ba1 from Bacillus thuringiensis subsp. medellin and B. thuringiensis subsp. israelensis synergize Bacillus sphaericus against Aedes aegypti and Resistant Culex quinquefasciatus (Diptera: Culicidae)","volume":"67","author":"Wirth","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.peptides.2012.05.023","article-title":"Cyt toxins produced by Bacillus thuringiensis: A protein fold conserved in several pathogenic microorganisms","volume":"41","author":"Bravo","year":"2013","journal-title":"Peptides"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"18303","DOI":"10.1073\/pnas.0505494102","article-title":"Bacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor","volume":"102","author":"Sun","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1271\/bbb.60140","article-title":"Cytolytic Toxin Cyt1aa of Bacillus thuringiensis Synergizes the Mosquitocidal Toxin Mtx1 of Bacillus sphaericus","volume":"70","author":"Zhang","year":"2006","journal-title":"Biosci. Biotech. Bioch."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1038\/s41598-018-22740-9","article-title":"Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity","volume":"8","author":"Pacheco","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/0022-2011(86)90158-8","article-title":"Insect toxicity of Bacillus thuringiensis isolated from soils of Japan","volume":"47","author":"Ohba","year":"1986","journal-title":"J. Invertebr. Pathol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s12038-018-9759-0","article-title":"A parasporin from Bacillus thuringiensis native to Peninsular India induces apoptosis in cancer cells through intrinsic pathway","volume":"43","author":"Chubika","year":"2018","journal-title":"J. Biosci."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1046\/j.1365-2672.1999.00692.x","article-title":"Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: In vitro cell-killing action on human cancer cells","volume":"86","author":"Mizuki","year":"1999","journal-title":"J. Appl. Microbiol."},{"key":"ref_96","unstructured":"Okumura, S., Ohba, M., Mizuki, E., Crickmore, N., C\u00f4t\u00e9, J.C., Nagamatsu, Y., Kitada, S., Sakai, H., Harata, K., and Shin, T. (2020, April 03). Parasporin nomenclature. Available online: http:\/\/parasporin.fitc.pref.fukuoka.jp\/list.html."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1046\/j.1365-2672.2000.01087.x","article-title":"In vitro cytotoxicity of non-Cyt inclusion proteins of a Bacillus thuringiensis isolate against human cells, including cancer cells","volume":"89","author":"Kim","year":"2000","journal-title":"J. Appl. Microbiol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1006\/bbrc.2000.2765","article-title":"Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells","volume":"272","author":"Lee","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1128\/CDLI.7.4.625-634.2000","article-title":"Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis","volume":"7","author":"Mizuki","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1139\/w00-084","article-title":"Characterization of the anti-cancer-cell parasporal proteins of a Bacillus thuringiensis isolate","volume":"46","author":"Yamashita","year":"2000","journal-title":"Can. J. Microbiol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0167-4838(01)00169-8","article-title":"A 28-kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity","volume":"1547","author":"Lee","year":"2001","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_102","first-page":"61","article-title":"Cytotoxicity of Bacillus thuringiensis crystal protein against mammalian cells","volume":"36","author":"Yamagiwa","year":"2002","journal-title":"Mem. Fac. Eng. Okayama Univ."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0304-4165(03)00102-8","article-title":"The cytotoxicity of Bacillus thuringiensis subsp. coreanensis A1519 strain against the human leukemic T cell","volume":"1622","author":"Namba","year":"2003","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_104","first-page":"2355","article-title":"Crystallization of parasporin-2, a Bacillus thuringiensis crystal protein with selective cytocidal activity against human cells","volume":"D60","author":"Akiba","year":"2004","journal-title":"Acta Cryst."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"21282","DOI":"10.1074\/jbc.M401881200","article-title":"Selective cytocidal action of a crystal protein of Bacillus thuringiensis on human cancer cells","volume":"279","author":"Ito","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1472-765X.2004.01544.x","article-title":"Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cell","volume":"39","author":"Okumura","year":"2004","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.1271\/bbb.69.2063","article-title":"A novel 29-kDa crystal protein from Bacillus thuringiensis induces caspase activation and cell death of Jurkat T cells","volume":"69","author":"Amano","year":"2005","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/jb\/mvi003","article-title":"Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis","volume":"137","author":"Katayama","year":"2005","journal-title":"J. Biochem."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"6313","DOI":"10.1021\/jf0506129","article-title":"Identification of a Novel Cytotoxic Protein, Cry45Aa, from Bacillus thuringiensis A1470 and Its Selective Cytotoxic Activity against Various Mammalian Cell Lines","volume":"53","author":"Okumura","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1093\/jb\/mvi177","article-title":"Typical three-domain Cry proteins of Bacillus thuringiensis strain A1462 exhibit cytocidal activity on limited human cancer cells","volume":"138","author":"Yamashita","year":"2005","journal-title":"J. Biochem."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.pep.2005.10.011","article-title":"Efficient Solubilization, activation and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as an Escherichia coli inclusion body","volume":"47","author":"Okumura","year":"2006","journal-title":"Protein Expres. Purif."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1271\/bbb.60352","article-title":"Investigation of a novel Bacillus thuringiensis gene encoding a parasporal protein, parasporin-4, that preferentially kills leukaemic T cells","volume":"70","author":"Saitoh","year":"2006","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/s00284-006-0351-8","article-title":"Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis","volume":"55","author":"Hayakawa","year":"2007","journal-title":"Curr. Microbiol."},{"key":"ref_114","first-page":"219","article-title":"Parasporin-4, A Novel Cancer Cell-killing Protein Produced by Bacillus thuringiensis","volume":"17","author":"Inouye","year":"2008","journal-title":"Food Sci. Biotechnol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S1387-2656(08)00009-4","article-title":"Identification and characterization of a novel cytotoxic protein, parasporin-4, produced by Bacillus thuringiensis A1470 strain","volume":"14","author":"Okumura","year":"2008","journal-title":"Biotechnol. Annu. Rev."},{"key":"ref_116","first-page":"91","article-title":"Parasporin-1Ab, a Novel Bacillus thuringiensis Cytotoxin Preferentially Active on Human Cancer Cells In Vitro","volume":"28","author":"Uemori","year":"2008","journal-title":"Anticancer Res."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1271\/bbb.90615","article-title":"Three Cry toxins in two types from Bacillus thuringiensis strain M019 preferentially kill human hepatocyte cancer and uterus cervix cancer cells","volume":"74","author":"Nagamatsu","year":"2010","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00284-013-0301-1","article-title":"Parasporin 1Ac2, a Novel Cytotoxic Crystal Protein Isolated from Bacillus thuringiensis B0462 Strain","volume":"66","author":"Kuroda","year":"2013","journal-title":"Curr. Microbiol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1007\/s10529-013-1275-6","article-title":"Identification of a second cytotoxic protein produced by Bacillus thuringiensis A1470","volume":"35","author":"Okumura","year":"2013","journal-title":"Biotechnol. Lett."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.3390\/toxins6061882","article-title":"Cloning and Characterization of a Unique Cytotoxic Protein Parasporin-5 Produced by Bacillus thuringiensis A1100 Strain","volume":"6","author":"Ekino","year":"2014","journal-title":"Toxins"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1139\/w05-134","article-title":"Occurrence of parasporin-producing Bacillus thuringiensis in Vietnam","volume":"52","author":"Yasutake","year":"2006","journal-title":"Can. J. Microbiol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1515\/znc-2008-1-225","article-title":"Identification of parasporin genes in Vietnamese isolates of Bacillus thuringiensis","volume":"63","author":"Yasutake","year":"2008","journal-title":"Zeitschrift fur Naturforschung C"},{"key":"ref_123","first-page":"545","article-title":"Characterization of parasporin gene harboring Indian isolates of Bacillus thuringiensis","volume":"4","author":"Lenina","year":"2014","journal-title":"Biotech"},{"key":"ref_124","first-page":"235","article-title":"Selective cytotoxic activity against leukemic cell lines from mosquitocidal Bacillus thuringiensis parasporal inclusions","volume":"39","author":"Nadarajah","year":"2008","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_125","first-page":"690","article-title":"Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875","volume":"48","author":"Zhu","year":"2008","journal-title":"Wei Sheng Wu xue Bao"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1007\/s00284-018-1479-z","article-title":"Anti-cancer Parasporin Toxins of New Bacillus thuringiensis Against Human Colon (HCT-116) and Blood (CCRF-CEM) Cancer Cell Lines","volume":"75","author":"Moazamian","year":"2018","journal-title":"Curr. Microbiol."},{"key":"ref_127","doi-asserted-by":"crossref","unstructured":"Aboul-Soud, M., Al-Amri, M.Z., Kumar, A., Al-Sheikh, Y.A., Ashour, A.E., and El-Kersh, T.A. (2019). Specific Cytotoxic Effects of Parasporal Crystal Proteins Isolated from Native Saudi Arabian Bacillus thuringiensis Strains against Cervical Cancer Cells. Molecules, 24.","DOI":"10.3390\/molecules24030506"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1365-2672.2006.03260.x","article-title":"Isolation and characterization of a novel Bacillus thuringiensis strain expressing a novel crystal protein with cytocidal activity against human cancer cells","volume":"103","author":"Jung","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1007\/s00284-011-9905-5","article-title":"Parasporins from a Caribbean Island: Evidence for a Globally dispersed Bacillus thuringiensis strain","volume":"62","author":"Granados","year":"2011","journal-title":"Curr. Microbiol."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1016\/j.bbamem.2010.11.003","article-title":"Mode of action of parasporin-4, a cytocidal protein from Bacillus thuringiensis","volume":"1808","author":"Okumura","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_131","unstructured":"Katayama, H., Kusaka, Y., and Mizuk, E. (2011). Parasporin-1 Receptor and Use Thereof. U.S. Patent 20110038880."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1038\/45257","article-title":"Induction of autophagy and inhibition of tumorigenesis by beclin 1","volume":"402","author":"Liang","year":"1999","journal-title":"Nature"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"26350","DOI":"10.1074\/jbc.M602589200","article-title":"Cytocidal actions of parasporin-2, an anti-tumor crystal toxin from Bacillus thuringiensis","volume":"281","author":"Kitada","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jmb.2008.12.002","article-title":"Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells","volume":"13","author":"Akiba","year":"2009","journal-title":"J. Mol. Biol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jip.2016.11.008","article-title":"Glycan region of GPI anchored-protein is required for cytocidal oligomerization of an anticancer parasporin-2, Cry46Aa1 protein, from Bacillus thuringiensis strain 3","volume":"142","author":"Abe","year":"2017","journal-title":"J. Invertebr. Pathol."},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Brasseur, K., Auger, P., Asselin, E., Parent, S., C\u00f4te, J.C., and Sirois, M. (2015). Parasporin-2 from a New Bacillus thuringiensis 4R2 Strain Induces Caspases Activation and Apoptosis in Human Cancer Cells. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135106"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1002\/pro.5560050805","article-title":"The (QxW)3 domain: A flexible lectin scaffold","volume":"5","author":"Hazes","year":"1996","journal-title":"Protein Sci."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1042\/BCJ20170122","article-title":"The human cancer cell active toxin Cry41Aa from Bacillus thuringiensis acts like its insecticidal counterparts","volume":"474","author":"Krishnan","year":"2017","journal-title":"Biochem. J."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0014-5793(96)00328-6","article-title":"Characterisation of the heptameric pore-forming complex of the Aeromonas toxin aerolysin using MALDI-TOF mass spectrometry","volume":"384","author":"Moniatte","year":"1996","journal-title":"FEBS Lett."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.tox.2009.07.016","article-title":"Parasporin-2 requires GPI-anchored proteins for the efficient cytocidal action to human hepatoma cells","volume":"264","author":"Kitada","year":"2009","journal-title":"Toxicology"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/j.1365-2958.1993.tb00962.x","article-title":"Crystalline bacterial cell surface layers","volume":"10","author":"Sleytr","year":"1993","journal-title":"Mol. Microbiol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1111\/1574-6976.12063","article-title":"S-layers: Principles and applications","volume":"38","author":"Sleytr","year":"2014","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1111\/j.1365-2672.2004.02365.x","article-title":"Expression of vip1\/vip2 genes in Escherichia coli and Bacillus thuringiensis and the analysis of their signal peptides","volume":"97","author":"Shi","year":"2004","journal-title":"J. Appl. Microbiol."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s00253-014-5966-2","article-title":"Genomic sequencing identifies novel Bacillus thuringiensis Vip1\/Vip2 binary and Cry8 toxins that have high toxicity to Scarabaeoidea larvae","volume":"99","author":"Bi","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_145","unstructured":"Carozzi, N.B., and Koziel, M. (1997). Vegetative insecticidal proteins: Novel proteins for control of corn pests. Advances in Insect Control, the Role of Transgenic Plants, Taylor & Francis Ltd."},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"937","DOI":"10.4014\/jmb.1105.05030","article-title":"Molecular characterization of a novel vegetative insecticidal protein from Bacillus thuringiensis effective against sap-sucking insect pest","volume":"21","author":"Sattar","year":"2011","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/protein\/gzn038","article-title":"From enzyme to zymogen: Engineering Vip2, an ADP-ribosyltransferase from Bacillus cereus, for conditional toxicity","volume":"21","author":"Jucovic","year":"2008","journal-title":"Protein Eng. Des. Sel."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1021\/bi051351z","article-title":"Vegetative insecticidal protein (Vip1Ac) of Bacillus thuringiensis HD201: Evidence for oligomer and channel formation","volume":"45","author":"Leuber","year":"2006","journal-title":"Biochemistry"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/MMBR.68.3.373-402.2004","article-title":"Binary bacterial toxins: Biochemistry, biology, and applications of common Clostridium and Bacillus proteins","volume":"68","author":"Barth","year":"2004","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_150","doi-asserted-by":"crossref","unstructured":"Bel, Y., Banyuls, N., Chakroun, M., Escriche, B., and Ferr\u00e9, J. (2017). Insights into the Structure of the Vip3Aa Insecticidal Protein by Protease Digestion Analysis. Toxins, 4.","DOI":"10.3390\/toxins9040131"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jip.2011.05.014","article-title":"Investigation of the steps involved in the difference of susceptibility of Ephesitia kuehniella and Spodoptera littoralis to the Bacillus thuringiensis Vip3Aa16 toxin","volume":"107","author":"Boukedi","year":"2011","journal-title":"J. Invertebr. Path."},{"key":"ref_152","first-page":"211","article-title":"New Combinations of Cry Genes From Bacillus Thuringiensis Strains Isolated From Northwestern Mexico","volume":"15","author":"Mendoza","year":"2012","journal-title":"Int. Microbiol."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.2174\/1389201020666190802114739","article-title":"Cry1A Proteins are Cytotoxic to HeLa but not to SiHa Cervical Cancer Cells","volume":"20","author":"Olmos","year":"2019","journal-title":"Curr. Pharm. Biotechnol."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/5\/301\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:26:02Z","timestamp":1760174762000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/5\/301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,6]]},"references-count":153,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["toxins12050301"],"URL":"https:\/\/doi.org\/10.3390\/toxins12050301","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,6]]}}}