{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:53:57Z","timestamp":1781920437175,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T00:00:00Z","timestamp":1592438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The noble false widow spider Steatoda nobilis originates from the Macaronesian archipelago and has expanded its range globally. Outside of its natural range, it may have a negative impact on native wildlife, and in temperate regions it lives in synanthropic environments where it frequently encounters humans, subsequently leading to envenomations. S. nobilis is the only medically significant spider in Ireland and the UK, and envenomations have resulted in local and systemic neurotoxic symptoms similar to true black widows (genus Latrodectus). S. nobilis is a sister group to Latrodectus which possesses the highly potent neurotoxins called \u03b1-latrotoxins that can induce neuromuscular paralysis and is responsible for human fatalities. However, and despite this close relationship, the venom composition of S. nobilis has never been investigated. In this context, a combination of transcriptomic and proteomic cutting-edge approaches has been used to deeply characterise S. nobilis venom. Mining of transcriptome data for the peptides identified by proteomics revealed 240 annotated sequences, of which 118 are related to toxins, 37 as enzymes, 43 as proteins involved in various biological functions, and 42 proteins without any identified function to date. Among the toxins, the most represented in numbers are \u03b1-latrotoxins (61), \u03b4-latroinsectotoxins (44) and latrodectins (6), all of which were first characterised from black widow venoms. Transcriptomics alone provided a similar representation to proteomics, thus demonstrating that our approach is highly sensitive and accurate. More precisely, a relative quantification approach revealed that latrodectins are the most concentrated toxin (28%), followed by \u03b1-latrotoxins (11%), \u03b4-latroinsectotoxins (11%) and \u03b1-latrocrustotoxins (11%). Approximately two-thirds of the venom is composed of Latrodectus-like toxins. Such toxins are highly potent towards the nervous system of vertebrates and likely responsible for the array of symptoms occurring after envenomation by black widows and false widows. Thus, caution should be taken in dismissing S. nobilis as harmless. This work paves the way towards a better understanding of the competitiveness of S. nobilis and its potential medical importance.<\/jats:p>","DOI":"10.3390\/toxins12060402","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T12:21:46Z","timestamp":1592482906000},"page":"402","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Venomics Approach Reveals a High Proportion of Lactrodectus-Like Toxins in the Venom of the Noble False Widow Spider Steatoda nobilis"],"prefix":"10.3390","volume":"12","author":[{"given":"John P.","family":"Dunbar","sequence":"first","affiliation":[{"name":"Venom Systems &amp; Proteomics Lab, School of Natural Sciences, Ryan Institute, National University of Ireland Galway, H91 TK33 Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2210-7234","authenticated-orcid":false,"given":"Antoine","family":"Fort","sequence":"additional","affiliation":[{"name":"Plant Systems Biology Lab, Plant and AgriBiosciences Research Centre, School of Natural Sciences, Ryan Institute, National University of Ireland Galway, H91 TK33 Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Redureau","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Laboratory, MolSys RU, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan","family":"Sulpice","sequence":"additional","affiliation":[{"name":"Plant Systems Biology Lab, Plant and AgriBiosciences Research Centre, School of Natural Sciences, Ryan Institute, National University of Ireland Galway, H91 TK33 Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel M.","family":"Dugon","sequence":"additional","affiliation":[{"name":"Venom Systems &amp; Proteomics Lab, School of Natural Sciences, Ryan Institute, National University of Ireland Galway, H91 TK33 Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8153-9590","authenticated-orcid":false,"given":"Lo\u00efc","family":"Quinton","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Laboratory, MolSys RU, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lyons, K., Dugon, M.M., and Healy, K. (2020). Diet Breadth Mediates the Prey Specificity of Venom Potency in Snakes. Toxins, 12.","DOI":"10.3390\/toxins12020074"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Whiteley, G., Logan, R.A., Leung, K.Y., Newberry, F.J., Rowley, P.D., Dunbar, J.P., Wagstaff, S.C., Casewell, N.R., and Harrison, R.A. (2016). Stabilising the Integrity of Snake Venom mRNA Stored under Tropical Field Conditions Expands Research Horizons. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004615"},{"key":"ref_3","first-page":"18","article-title":"Proteolytic Activity of Elapid and Viperid Snake Venoms and Its Implication to Digestion","volume":"1","author":"Bottrall","year":"2010","journal-title":"J. Venom Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.toxicon.2012.04.350","article-title":"Sphingomyelinase D in Sicariid Spider Venom is a Potent Insecticidal Toxin","volume":"60","author":"Kerins","year":"2012","journal-title":"Toxicon"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1134\/S0006297909130069","article-title":"Molecular Diversity of Spider Venom","volume":"74","author":"Vassilevski","year":"2009","journal-title":"Biochemistry (Moscow)"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kuhn-Nentwig, L., Langenegger, N., Heller, M., Koua, D., and Nentwig, W. (2019). The Dual Prey-Inactivation Strategy of Spiders\u2014In-Depth Venomic Analysis of Cupiennius salei. Toxins, 11.","DOI":"10.3390\/toxins11030167"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1093\/molbev\/mst011","article-title":"Molecular Evolution of \u03b1-Latrotoxin, the Exceptionally Potent Vertebrate Neurotoxin in Black Widow Spider Venom","volume":"30","author":"Garb","year":"2013","journal-title":"Molecular Biol. Evol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3897\/neobiota.42.31582","article-title":"Steatoda nobilis, a False Widow on the Rise: A Synthesis of Past and Current Distribution Trends","volume":"42","author":"Bauer","year":"2019","journal-title":"NeoBiota"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1080\/15563650.2017.1393084","article-title":"Envenomation by the Noble False Widow Spider Steatoda nobilis (Thorell, 1875)\u2013Five New Cases of Steatodism from Ireland and Great Britain","volume":"56","author":"Dunbar","year":"2018","journal-title":"Clin. Toxicol."},{"key":"ref_10","first-page":"237","article-title":"Primer Registro de Una Mordedura De Steatoda nobilis (Thorell, 1875) (Arachnida: Araneae: Theridiidae) en Chile","volume":"15","year":"2016","journal-title":"Arquivos Entomol\u00f3xicos"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/0041-0101(91)90198-Z","article-title":"Neurotoxic Envenoming by an Immigrant Spider (Steatoda nobilis) in Southern England","volume":"29","author":"Warrell","year":"1991","journal-title":"Toxicon"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3318\/bioe.2017.11","article-title":"Occurrence, reproductive rate and identification of the non-native noble false widow spider Steatoda nobilis (Thorell, 1875) in Ireland","volume":"117","author":"Dugon","year":"2017","journal-title":"Biol. Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1353\/bae.2018.0014","article-title":"Biting off more than one can chew: First record of the non-native noble false widow spider Steatoda nobilis (Thorell, 1875) feeding on the native viviparous lizard Zootoca vivipara","volume":"118B","author":"Dunbar","year":"2018","journal-title":"Biol. Environ."},{"key":"ref_14","first-page":"39","article-title":"New Irish Record for Steatoda triangulosa (Walckenaer, 1802), and New County Records for Steatoda nobilis (Thorell, 1875), Steatoda bipunctata (Linnaeus, 1758) and Steatoda grossa (CL Koch, 1838)","volume":"36","author":"Dunbar","year":"2018","journal-title":"Ir. Nat. J."},{"key":"ref_15","first-page":"200","article-title":"Three Spiders (Araneae) New to Ireland: Bolyphantes alticeps, Oonops domesticus and Steatoda nobilis","volume":"26","author":"Nolan","year":"1999","journal-title":"Ir. Nat. J."},{"key":"ref_16","first-page":"112","article-title":"The Comb-Footed Spider Fauna of the Central Anatolia Region and New Records for the Turkish Fauna (Araneae: Theridiidae)","volume":"10","author":"Mergen","year":"2007","journal-title":"Serket"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1080\/21658005.2015.1068508","article-title":"New Data on Spider Fauna of Iran (Arachnida: Araneae), Part II","volume":"25","author":"Zamani","year":"2015","journal-title":"Zool. Ecol."},{"key":"ref_18","first-page":"52","article-title":"Nuevos Registros de Steatoda nobilis (Thorell, 1875)(Araneae: Theridiidae) de Sudam\u00e9rica","volume":"33","author":"Carvajal","year":"2018","journal-title":"Revista Ib\u00e9rica de Aracnolog\u00eda"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"479","DOI":"10.4039\/tce.2015.83","article-title":"Steatoda nobilis (Araneae: Theridiidae) in South America: A New Alien Species for Chile","volume":"148","author":"Mardones","year":"2016","journal-title":"Can. Entomol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"98","DOI":"10.3956\/2014-91.1.098","article-title":"The European Spider Steatoda nobilis (Thorell, 1875)(Araneae: Theridiidae) becoming Widespread in California","volume":"91","author":"Vetter","year":"2015","journal-title":"Pan-Pac. Entomol."},{"key":"ref_21","first-page":"164","article-title":"The Theridiid Spider Steatoda nobilis (Thorell, 1875) in Britain","volume":"9","author":"Snazell","year":"1993","journal-title":"Bull. Br. Arachnol. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.13156\/arac.2012.15.1.269","article-title":"New Records of Steatoda nobilis (Thorell, 1875)(Araneae, Theridiidae), an Introduced Species on the Italian Mainland and in Sardinia","volume":"15","author":"Kulczycki","year":"2012","journal-title":"Arachnology"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Gendreau, K.L., Haney, R.A., Schwager, E.E., Wierschin, T., Stanke, M., Richards, S., and Garb, J.E. (2017). House Spider Genome uncovers Evolutionary Shifts in the Diversity and Expression of Black Widow Venom Proteins Associated with Extreme Toxicity. BMC Genom., 18.","DOI":"10.1186\/s12864-017-3551-7"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Haney, R.A., Ayoub, N.A., Clarke, T.H., Hayashi, C.Y., and Garb, J.E. (2014). Dramatic Expansion of the Black Widow Toxin Arsenal uncovered by Multi-Tissue Transcriptomics and Venom Proteomics. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-366"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/71247","article-title":"Structure of \u03b1-Latrotoxin Oligomers Reveals that Divalent Cation-Dependent Tetramers form Membrane Pores","volume":"7","author":"Orlova","year":"2000","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_26","unstructured":"Ushkaryov, Y.A., Rohou, A., and Sugita, S. (2008). receptors. Pharmacology of Neurotransmitter Release, Springer."},{"key":"ref_27","first-page":"49","article-title":"Predation on a Weasel Skink (Saproscincus mustelinus)(Squamata: Scincidae: Lygosominae) by a Redback Spider (Latrodectus hasselti)(Araneae: Araneomorpha: Theridiidae), with a Review of other Latrodectus Predation Events Involving Squamates","volume":"44","author":"Kelly","year":"2017","journal-title":"Herpetofauna"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.toxicon.2017.02.014","article-title":"Diversity in Sequences, Post-Translational Modifications and Expected Pharmacological Activities of Toxins from Four Conus Species Revealed by the Combination of Cutting-Edge Proteomics, Transcriptomics and Bioinformatics","volume":"130","author":"Degueldre","year":"2017","journal-title":"Toxicon"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103707","DOI":"10.1016\/j.jprot.2020.103707","article-title":"Venomics of the asp viper Vipera aspis aspis from France","volume":"218","author":"Giribaldi","year":"2020","journal-title":"J. Proteom."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1074\/mcp.M500230-MCP200","article-title":"Absolute Quantification of Proteins by LCMSE: A Virtue of Parallel MS Acquisition","volume":"5","author":"Silva","year":"2006","journal-title":"Mol. Cell. Proteom."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1093\/bioinformatics\/btx661","article-title":"ArachnoServer 3.0: An Online Resource for Automated Discovery, Analysis and Annotation of Spider Toxins","volume":"34","author":"Pineda","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_32","unstructured":"Casas, J. (2011). Spider Physiology and Behaviour: Behaviour, Academic Press."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1111\/j.1471-4159.1990.tb05793.x","article-title":"\u03b1-Latrotoxin Releases both Vesicular and Cytoplasmic Glutamate from Isolated Nerve Terminals","volume":"55","author":"McMahon","year":"1990","journal-title":"J. Neurochem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mackessy, S.P. (2016). Handbook of Venoms and Toxins of Reptiles, CRC Press.","DOI":"10.1201\/9781420008661"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1074\/jbc.M117.810911","article-title":"Identification of a Precursor Processing Protease from the Spider Cupiennius salei Essential for Venom Neurotoxin Maturation","volume":"293","author":"Langenegger","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_36","first-page":"13","article-title":"The behavioural ecology of Latrodectus hasselti (Thorell), the Australian Redback Spider (Araneae: Theridiidae): A Review","volume":"52","author":"Forster","year":"1995","journal-title":"Rec. West. Aust. Mus."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.toxicon.2014.11.229","article-title":"Do Spiders Vector Bacteria during Bites? The Evidence Indicates Otherwise","volume":"93","author":"Vetter","year":"2015","journal-title":"Toxicon"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1146\/annurev.neuro.24.1.933","article-title":"\u03b1-Latrotoxin and Its Receptors: Neurexins and CIRL\/Latrophilins","volume":"24","year":"2001","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.biochi.2016.06.003","article-title":"Expression and immunological cross-reactivity of LALP3, a novel astacin-like metalloprotease from brown spider (Loxosceles intermedia) venom","volume":"128","author":"Morgon","year":"2016","journal-title":"Biochimie"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"s13742-015-0089-y","DOI":"10.1186\/s13742-015-0089-y","article-title":"Rcorrector: Efficient and Accurate Error Correction for Illumina RNA-seq Reads","volume":"4","author":"Song","year":"2015","journal-title":"GigaScience"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast Gapped-Read Alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Trinity: Reconstructing a Full-Length Transcriptome without a Genome from RNA-Seq Data","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De Novo Transcript Sequence Reconstruction from RNA-seq Using the Trinity Platform for Reference Generation and Analysis","volume":"8","author":"Haas","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_45","unstructured":"Haas, B., and Papanicolaou, A. (2018, May 17). TransDecoder (Find Coding Regions within Transcripts). Github, nd. Available online: https:\/\/github.com\/TransDecoder\/TransDecoder."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.toxicon.2017.01.014","article-title":"Venom-Gland Transcriptomics and Venom Proteomics of the Black-Back Scorpion (Hadrurus spadix) Reveal Detectability Challenges and an Unexplored Realm of Animal Toxin Diversity","volume":"128","author":"Rokyta","year":"2017","journal-title":"Toxicon"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.celrep.2016.12.063","article-title":"A Tissue-Mapped Axolotl de Novo Transcriptome Enables Identification of Limb Regeneration Factors","volume":"18","author":"Bryant","year":"2017","journal-title":"Cell Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"D506","DOI":"10.1093\/nar\/gky1049","article-title":"UniProt: A Worldwide Hub of Protein Knowledge","volume":"47","author":"Consortium","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_49","first-page":"D427","article-title":"The Pfam Protein Families Database in 2019","volume":"47","author":"Mistry","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/nbt.3519","article-title":"Near-Optimal Probabilistic RNA-seq Quantification","volume":"34","author":"Bray","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_51","first-page":"1","article-title":"Goseq: Gene Ontology Testing for RNA-seq Datasets","volume":"8","author":"Young","year":"2012","journal-title":"R Bioconduct."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Supek, F., Bo\u0161njak, M., \u0160kunca, N., and \u0160muc, T. (2011). REVIGO Summarizes and Visualizes Long Lists of Gene Ontology Terms. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021800"},{"key":"ref_53","unstructured":"Development Core Team, R. (2011). R: A Language and Environment for Statistical Computing, R foundation for Statistical Computing."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1145\/571647.571649","article-title":"Ordered and Quantum Treemaps: Making Effective Use of 2D Space to Display Hierarchies","volume":"21","author":"Bederson","year":"2002","journal-title":"AcM Trans. Graph. (TOG)"}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/6\/402\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:40:13Z","timestamp":1760175613000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/6\/402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,18]]},"references-count":54,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["toxins12060402"],"URL":"https:\/\/doi.org\/10.3390\/toxins12060402","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,18]]}}}