{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T18:44:09Z","timestamp":1783363449894,"version":"3.54.6"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Chongqing, China","award":["cstc2018jcyjA0834"],"award-info":[{"award-number":["cstc2018jcyjA0834"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>In practical dairy production, cows are frequently subjected to inflammatory diseases, such as high-grain diet-induced subacute ruminal acidosis (SARA) as well as mastitis and metritis. Under the circumstances, lipopolysaccharide (LPS) induces oxidative stress within the cow and in the mammary epithelial cells. It has implications in practical production to alleviate oxidative stress and to optimize the lactational function of the mammary epithelial cells. This study thus aimed to investigate the antioxidative effects of dandelion aqueous extract (DAE) on LPS-induced oxidative stress and the mechanism of DAE as an antioxidant to alleviate oxidative stress through the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in the bovine mammary epithelial cell line MAC-T cells. The cells were cultured for 48 h in six treatments including control (without LPS and DAE), LPS (100 ng\/mL), DAE10 (100 ng\/mL LPS and 10 \u03bcg\/mL DAE), DAE50 (100 ng\/mL LPS and 50 \u03bcg\/mL DAE), DAE100 (100 ng\/mL LPS and 100 \u03bcg\/mL DAE), and DAE200 (100 ng\/mL LPS and 200 \u03bcg\/mL DAE), respectively. The results showed that cell viability was reduced by LPS, and the adverse effect of LPS was suppressed with the supplementation of DAE. Lipopolysaccharide-induced oxidative stress through enhancing reactive oxygen species (ROS) production, resulted in increases in oxidative damage marker concentrations, while 10 and 50 \u03bcg\/mL DAE alleviated the LPS-induced oxidative stress via scavenging cellular ROS and improving antioxidant enzyme activity. The upregulation of antioxidative gene expression in DAE treatments was promoted through activating the Nrf2 signaling pathway, with DAE at a concentration of 50 \u03bcg\/mL exhibiting the highest effect. Overall, DAE acted as an effective antioxidant to inhibit LPS-induced oxidative stress and as a potential inducer of the Nrf2 signaling pathway.<\/jats:p>","DOI":"10.3390\/toxins12080496","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T07:45:57Z","timestamp":1596440757000},"page":"496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Dandelion Extract Alleviated Lipopolysaccharide-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1487-3852","authenticated-orcid":false,"given":"Yawang","family":"Sun","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjiang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zili","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncai","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-3050","authenticated-orcid":false,"given":"Guozhong","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Southwest University, Chongqing 400716, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1152\/physrev.00018.2001","article-title":"Free radicals in the radicals in the physiological control of cell function","volume":"82","year":"2002","journal-title":"Physiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1007\/s10495-012-0740-3","article-title":"Low concentrations of isothiocyanates protect mesenchymal stem cells from oxidative injuries, while hi concentrations exacerbate DNA damage","volume":"17","author":"Zanichelli","year":"2012","journal-title":"Apoptosis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"666","DOI":"10.3168\/jds.2016-10871","article-title":"Lipopolysaccharide derived from the digestive tract provokes oxidative stress in the liver of dairy cows fed a high-grain diet","volume":"100","author":"Abaker","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1038\/ni1112","article-title":"Toll-like receptor control of the adaptive immune responses","volume":"5","author":"Iwasaki","year":"2004","journal-title":"Nat. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/s12917-016-0755-z","article-title":"Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet","volume":"12","author":"Jin","year":"2016","journal-title":"BMC Vet. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2370","DOI":"10.1002\/jcb.27565","article-title":"Sodium butyrate ameliorates lipopolysaccharide-induced cow mammary epithelial cells from oxidative stress damage and apoptosis","volume":"120","author":"Li","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.freeradbiomed.2020.01.016","article-title":"Butyrate alleviates oxidative stress by regulating NRF2 nuclear accumulation and H3K9\/14 acetylation via GPR109A in bovine mammary epithelial cells and mammary glands","volume":"152","author":"Guo","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.jep.2006.07.021","article-title":"Taraxacum: A review on its phytochemical and pharmacological profile","volume":"107","author":"Carle","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/j.1753-4887.2012.00509.x","article-title":"Diverse biological activities of dandelion","volume":"70","author":"Visioli","year":"2012","journal-title":"Nutr. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1006\/abbi.1996.0395","article-title":"The interaction of NADPH-P450 reductase with P450: An electrochemical study of the role of the flavin mononucleotide-binding domain","volume":"333","author":"Estabrook","year":"1996","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1021\/jf0258858","article-title":"Antioxidant, prooxidant, and cytotoxic activities of solvent fractionated dandelion (Taraxacum officinale) flower extracts in vitro","volume":"51","author":"Hu","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.phymed.2003.12.012","article-title":"Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro","volume":"12","author":"Hu","year":"2005","journal-title":"Phytomedicine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jff.2019.05.019","article-title":"Dandelion (Taraxacum officinale L.) root components exhibit anti-oxidative and antiplatelet action in an in vitro study","volume":"59","author":"Lis","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.jep.2010.11.015","article-title":"Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-\u03baB modulation in RAW 264.7 cells","volume":"133","author":"Park","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3390\/ijms11010067","article-title":"Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits","volume":"11","author":"Choi","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"ref_16","first-page":"54","article-title":"Invivo antioxidant activity of hydroalcoholic extract of Taraxacum officinale roots in rats","volume":"38","author":"Sumanth","year":"2006","journal-title":"Res. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.fsi.2017.12.020","article-title":"Effects of dietary dandelion extract on intestinal morphology, antioxidant status, immune function and physical barrier function of juvenile golden pompano Trachinotus ovatus","volume":"73","author":"Tan","year":"2018","journal-title":"Fish Shellfish Immun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.stem.2010.12.006","article-title":"Redox regulation by Keap1 and Nrf2 controls intestinal stem cell proliferation in Drosophila","volume":"8","author":"Hochmuth","year":"2011","journal-title":"Cell Stem Cell."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.bbamcr.2018.02.010","article-title":"Nrf2-Keap1 signaling in oxidative and reductive stress","volume":"1865","author":"Bellezza","year":"2018","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9719618","DOI":"10.1155\/2019\/9719618","article-title":"The role of Nrf2 signaling pathway in Eucommia ulmoides flavones regulating oxidative stress in the intestine of piglets","volume":"2019","author":"Xiao","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1462-5822.2005.00678.x","article-title":"Ringing the alarm bells: Signaling and apoptosis in influenza virus infected cells","volume":"8","author":"Ludwig","year":"2006","journal-title":"Cell. Microbiol."},{"key":"ref_22","first-page":"120","article-title":"Lower Concentrations of blueberry polyphenolic-rich extract differentially alter HepG2 cell proliferation and expression of genes related to cell-cycle, oxidation and epigenetic machinery","volume":"3","author":"Grusak","year":"2012","journal-title":"J. Nutr. Disord. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.vetimm.2008.10.305","article-title":"Impact of oxidative stress on the health and immune function of dairy cattle","volume":"128","author":"Sordillo","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e0178525","DOI":"10.1371\/journal.pone.0178525","article-title":"The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells","volume":"12","author":"Yu","year":"2017","journal-title":"PLoS ONE"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12011-019-01961-7","article-title":"The protective effect of selenium on the lipopolysaccharide-induced oxidative stress and depressed gene expression related to milk protein synthesis in bovine mammary epithelial cells","volume":"197","author":"Zhang","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1097\/01.shk.0000194719.82845.39","article-title":"Attenuation of endotoxin-induced oxidative stress and multiple organ injury by 3,4-methylenedioxyphenol in rats","volume":"25","author":"Hsu","year":"2006","journal-title":"Shock"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"59S","DOI":"10.1111\/j.1530-0277.2000.tb00014.x","article-title":"Glutathione depletion enhances the formation of superoxide anion released into hepatic sinusoids after lipopolysaccharide challenge","volume":"24","author":"Moriya","year":"2000","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.freeradbiomed.2004.10.013","article-title":"Gene expression profiling of Nrf2-mediated protection against oxidative injury","volume":"38","author":"Cho","year":"2005","journal-title":"Free Radic. Biol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2572175","DOI":"10.1155\/2016\/2572175","article-title":"Protection of bovine mammary epithelial cells from hydrogen peroxide-induced oxidative cell damage by resveratrol","volume":"2016","author":"Jin","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1146\/annurev-pharmtox-011112-140320","article-title":"Role of Nrf2 in oxidative stress and toxicity","volume":"53","author":"Ma","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ijbiomac.2019.05.082","article-title":"Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress","volume":"135","author":"Wen","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1093\/jas\/sky037","article-title":"Vitamin A pretreatment protects NO-induced bovine mammary epithelial cells from oxidative stress by modulating Nrf2 and NF-\u03baB signaling pathways","volume":"96","author":"Shi","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1021\/jf304768p","article-title":"Dihydroquercetin (DHQ) induced HO-1 and NQO-1 expression against oxidative stress through the Nrf2-dependent antioxidant pathway","volume":"61","author":"Liang","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"44765","DOI":"10.1074\/jbc.M208704200","article-title":"Electrophile response element-mediated induction of the cyctine\/glutamate exchange transporter gene expression","volume":"227","author":"Sasaki","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1042\/bj20030754","article-title":"Identification of a novel Nrf2-regulated antioxidant response element (ARE) in the mouse NAD(P)H:quinone oxidoreductase 1 gene: Reassessement of the ARE consensus sequence","volume":"374","author":"Nioi","year":"2003","journal-title":"Biochem. J."},{"key":"ref_36","first-page":"103","article-title":"Dandelion polyphenols protect against acetaminophen-induced hepatotoxicity in mice via activation of the Nrf2\/Ho-1 pathway and inhibition of the JNK signaling pathway","volume":"18","author":"Ren","year":"2020","journal-title":"Chin. J. Nat. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s12906-016-1520-3","article-title":"Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs","volume":"17","author":"Hu","year":"2017","journal-title":"BMC Complement. Altern. Med."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/8\/496\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:53:42Z","timestamp":1760176422000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/12\/8\/496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,1]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["toxins12080496"],"URL":"https:\/\/doi.org\/10.3390\/toxins12080496","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,1]]}}}