{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T10:08:38Z","timestamp":1783850918813,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN202619"],"award-info":[{"award-number":["IN202619"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["PRONACE 303045.P"],"award-info":[{"award-number":["PRONACE 303045.P"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Vicerrector\u00eda de Investigaciones, Universidad del Valle, Colombia","award":["Movilidad Internacional CIAM"],"award-info":[{"award-number":["Movilidad Internacional CIAM"]}]},{"name":"Vicerrector\u00eda de Investigaciones, Universidad del Cauca,Colombia","award":["Movilidad Internacional"],"award-info":[{"award-number":["Movilidad Internacional"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The Colombian scorpion Centruroides margaritatus produces a venom considered of low toxicity. Nevertheless, there are known cases of envenomation resulting in cardiovascular disorders, probably due to venom components that target ion channels. Among them, the humanether-\u00e0-go-go-Related gene (hERG1) potassium channels are critical for cardiac action potential repolarization and alteration in its functionality are associated with cardiac disorders. This work describes the purification and electrophysiological characterization of a Centruroides margaritatus venom component acting on hERG1 channels, the CmERG1 toxin. This novel peptide is composed of 42 amino acids with a MW of 4792.88 Da, folded by four disulfide bonds and it is classified as member number 10 of the \u03b3-KTx1 toxin family. CmERG1 inhibits hERG1 currents with an IC50 of 3.4 \u00b1 0.2 nM. Despite its 90.5% identity with toxin \u0263-KTx1.1, isolated from Centruroides noxius, CmERG1 completely blocks hERG1 current, suggesting a more stable plug of the hERG channel, compared to that formed by other \u0263-KTx.<\/jats:p>","DOI":"10.3390\/toxins13060407","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T21:16:58Z","timestamp":1623187018000},"page":"407","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Colombian Scorpion Centruroides margaritatus: Purification and Characterization of a Gamma Potassium Toxin with Full-Block Activity on the hERG1 Channel"],"prefix":"10.3390","volume":"13","author":[{"given":"Jos\u00e9","family":"Beltr\u00e1n-Vidal","sequence":"first","affiliation":[{"name":"Grupo de Investigaciones Herpetol\u00f3gicas y Toxinol\u00f3gicas, Centro de Investigaciones Biom\u00e9dicas, Departamento de Biolog\u00eda, Facultad de Ciencias Naturales, Exactas y de la Educaci\u00f3n, Universidad del Cauca, Sector Tulcan, Calle 2 N 3N-100, Popay\u00e1n 190002, Cauca, Colombia"},{"name":"Grupo de Investigaci\u00f3n Laboratorio de Herpetolog\u00eda y Toxinolog\u00eda, Departamento de Fisiolog\u00eda, Facultad de Salud, Universidad del Valle, Calle 4B N\u00b0 36-00, Santiago de Cali 760043, Valle del Cauca, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Carcamo-Noriega","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Av. Universidad 2001, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7755-2936","authenticated-orcid":false,"given":"Nina","family":"Pastor","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Din\u00e1mica Celular, Research Institute in Basic and Applied Sciences (IICBA), Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Zamudio-Zu\u00f1iga","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Av. Universidad 2001, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0193-5508","authenticated-orcid":false,"given":"Jimmy Alexander","family":"Guerrero-Vargas","sequence":"additional","affiliation":[{"name":"Grupo de Investigaciones Herpetol\u00f3gicas y Toxinol\u00f3gicas, Centro de Investigaciones Biom\u00e9dicas, Departamento de Biolog\u00eda, Facultad de Ciencias Naturales, Exactas y de la Educaci\u00f3n, Universidad del Cauca, Sector Tulcan, Calle 2 N 3N-100, Popay\u00e1n 190002, Cauca, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Casta\u00f1o","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Laboratorio de Herpetolog\u00eda y Toxinolog\u00eda, Departamento de Fisiolog\u00eda, Facultad de Salud, Universidad del Valle, Calle 4B N\u00b0 36-00, Santiago de Cali 760043, Valle del Cauca, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourival Domingos","family":"Possani","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Av. Universidad 2001, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-5303","authenticated-orcid":false,"given":"Rita","family":"Restano-Cassulini","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Av. 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