{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:58:55Z","timestamp":1790823535795,"version":"4.1.0"},"reference-count":61,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T00:00:00Z","timestamp":1630108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000869"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000869"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Czech Ministry of Education, Youth and Sports","award":["LM2018121"],"award-info":[{"award-number":["LM2018121"]}]},{"name":"Operational Program Research, Development and Innovation","award":["CZ.02.1.01\/0.0\/0.0\/17_043\/0009632"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17_043\/0009632"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Fish are exposed to numerous stressors in the environment including pollution, bacterial and viral agents, and toxic substances. Our study with common carps leveraged an integrated approach (i.e., histology, biochemical and hematological measurements, and analytical chemistry) to understand how cyanobacteria interfere with the impact of a model viral agent, Carp sprivivirus (SVCV), on fish. In addition to the specific effects of a single stressor (SVCV or cyanobacteria), the combination of both stressors worsens markers related to the immune system and liver health. Solely combined exposure resulted in the rise in the production of immunoglobulins, changes in glucose and cholesterol levels, and an elevated marker of impaired liver, alanine aminotransferase (ALT). Analytical determination of the cyanobacterial toxin microcystin-LR (MC-LR) and its structurally similar congener MC-RR and their conjugates showed that SVCV affects neither the levels of MC in the liver nor the detoxification capacity of the liver. MC-LR and MC-RR were depurated from liver mostly in the form of cysteine conjugates (MC-LR-Cys, MC-RR-Cys) in comparison to glutathione conjugates (LR-GSH, RR-GSH). Our study brought new evidence that cyanobacteria worsen the effect of viral agents. Such inclusion of multiple stressor concept helps us to understand how and to what extent the relevant environmental stressors co-influence the health of the fish population.<\/jats:p>","DOI":"10.3390\/toxins13090601","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T22:58:15Z","timestamp":1630450695000},"page":"601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cyanobacteria Microcystis aeruginosa Contributes to the Severity of Fish Diseases: A Study on Spring Viraemia of Carp"],"prefix":"10.3390","volume":"13","author":[{"given":"Miroslava","family":"Palikova","sequence":"first","affiliation":[{"name":"Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"},{"name":"Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agronomy, Mendel University in Brno, 61300 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radovan","family":"Kopp","sequence":"additional","affiliation":[{"name":"Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agronomy, Mendel University in Brno, 61300 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Kohoutek","sequence":"additional","affiliation":[{"name":"RECETOX (Research Centre for Toxic Compounds in the Environment), Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-7455","authenticated-orcid":false,"given":"Ludek","family":"Blaha","sequence":"additional","affiliation":[{"name":"RECETOX (Research Centre for Toxic Compounds in the Environment), Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Mares","sequence":"additional","affiliation":[{"name":"Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agronomy, Mendel University in Brno, 61300 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Ondrackova","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, 62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Papezikova","sequence":"additional","affiliation":[{"name":"Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"},{"name":"Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agronomy, Mendel University in Brno, 61300 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3224-7916","authenticated-orcid":false,"given":"Hana","family":"Minarova","sequence":"additional","affiliation":[{"name":"Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 61242 Brno, Czech Republic"},{"name":"Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, 62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubomir","family":"Pojezdal","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, 62100 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Adamovsky","sequence":"additional","affiliation":[{"name":"RECETOX (Research Centre for Toxic Compounds in the Environment), Faculty of Science, Masaryk University, 62500 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,28]]},"reference":[{"key":"ref_1","unstructured":"OIE (2016). Aquatic Animals Commission Spring Viraenia of Carps. Manual of Diagnostic Tests for Aquatic Animals, OIE."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/s41586-019-1648-7","article-title":"Widespread global increase in intense lake phytoplankton blooms since the 1980s","volume":"574","author":"Ho","year":"2019","journal-title":"Nature"},{"key":"ref_3","unstructured":"US EPA (2020, April 19). Harmful algal Blooms, Available online: http:\/\/www2.epa.gov\/nutrientpollution\/harmful-algal-blooms."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/978-0-387-75865-7_28","article-title":"Cyanobacterial poisoning in livestock, wild mammals and birds\u2014An overview","volume":"619","author":"Stewart","year":"2008","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.3390\/toxins7041374","article-title":"One Health and Cyanobacteria in Freshwater Systems: Animal Illnesses and Deaths Are Sentinel Events for Human Health Risks","volume":"7","author":"Hilborn","year":"2015","journal-title":"Toxins"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"12457","DOI":"10.1021\/acs.est.5b02049","article-title":"Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin","volume":"49","author":"Adamovsky","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.toxicon.2018.04.031","article-title":"Immunomodulatory effects of selected cyanobacterial peptides in vitro","volume":"149","author":"Moosova","year":"2018","journal-title":"Toxicon"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.chemosphere.2019.03.143","article-title":"Immunomodulatory effects of cyanobacterial toxin cylindrospermopsin on innate immune cells","volume":"226","author":"Moosova","year":"2019","journal-title":"Chemosphere"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2013.06.016","article-title":"In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters","volume":"73","author":"Palikova","year":"2013","journal-title":"Toxicon"},{"key":"ref_10","first-page":"77","article-title":"Combined exposure of carps (Cyprinus carpio L.) to cyanobacterial biomass and white spot disease","volume":"33","author":"Palikova","year":"2012","journal-title":"Neuro. Endocrinol. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2019.03.002","article-title":"Nonalcoholic fatty liver disease alters microcystin-LR toxicokinetics and acute toxicity","volume":"162","author":"Clarke","year":"2019","journal-title":"Toxicon"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Lad, A., Su, R., Breidenbach, J., Stemmer, P., Carruthers, N., Sanchez, N., Khalaf, F., Zhang, S., Kleinhenz, A., and Dube, P. (2019). Chronic Low Dose Oral Exposure to Microcystin-LR Exacerbates Hepatic Injury in a Murine Model of Non-Alcoholic Fatty Liver Disease. Toxins, 11.","DOI":"10.3390\/toxins11090486"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Su, R.C., Blomquist, T.M., Kleinhenz, A.L., Khalaf, F.K., Dube, P., Lad, A., Breidenbach, J.D., Mohammed, C.J., Zhang, S., and Baum, C.E. (2019). Exposure to the harmful algal bloom (HAB) toxin microcystin-lr (MC-LR) prolongs and increases severity of dextran sulfate sodium (DSS)-induced colitis. Toxins, 11.","DOI":"10.3390\/toxins11060371"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8742","DOI":"10.1038\/s41598-019-45009-1","article-title":"Environmental microcystin targets the microbiome and increases the risk of intestinal inflammatory pathology via NOX2 in underlying murine model of Nonalcoholic Fatty Liver Disease","volume":"9","author":"Sarkar","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Moosov\u00e1, Z., \u0160indlerov\u00e1, L., Ambr\u016fzov\u00e1, B., Ambro\u017eov\u00e1, G., Va\u0161\u00ed\u010dek, O., Velki, M., Babica, P., and Kubala, L. (2019). Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response. Toxins, 11.","DOI":"10.3390\/toxins11040218"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.envpol.2018.03.059","article-title":"Microcystin-leucine arginine (MC-LR) induces bone loss and impairs bone micro-architecture by modulating host immunity in mice: Implications for bone health","volume":"238","author":"Dar","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1002\/etc.87","article-title":"Microcystin-Lr Modulates Selected Immune Parameters and Induces Necrosis\/Apoptosis of Carp Leucocytes","volume":"29","author":"Rymuszka","year":"2010","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1177\/09603271060080075","article-title":"The influence of microcystin-LR on fish phagocytic cells","volume":"26","author":"Sieroslawska","year":"2007","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.mito.2017.11.005","article-title":"Regulation of B cell fate, survival, and function by mitochondria and autophagy","volume":"41","author":"Sandoval","year":"2018","journal-title":"Mitochondrion"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.4049\/jimmunol.1302062","article-title":"Metabolic Reprogramming Is Required for Antibody Production That Is Suppressed in Anergic but Exaggerated in Chronically BAFF-Exposed B Cells","volume":"192","author":"Wang","year":"2014","journal-title":"J. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s12291-013-0408-y","article-title":"Alkaline Phosphatase: An Overview","volume":"29","author":"Sharma","year":"2014","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.toxicon.2006.11.032","article-title":"Effects of cyanobacterial biomass on the Japanese quail","volume":"49","author":"Skocovska","year":"2007","journal-title":"Toxicon"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4984","DOI":"10.1016\/j.scitotenv.2010.07.050","article-title":"Combined exposure to cyanobacterial biomass, lead and the Newcastle virus enhances avian toxicity","volume":"408","author":"Pikula","year":"2010","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0166-445X(91)90012-X","article-title":"Histopathological Effects of Microcystin-Lr, a Cyclic Peptide Toxin from the Cyanobacterium (Blue-Green-Alga) Microcystis-Aeruginosa, on Common Carp (Cyprinus carpio L)","volume":"20","author":"Rabergh","year":"1991","journal-title":"Aquat. Toxicol."},{"key":"ref_25","first-page":"11","article-title":"Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass","volume":"34","author":"Adamovsky","year":"2013","journal-title":"Neuro Endocrinol. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"924","DOI":"10.4254\/wjh.v8.i22.924","article-title":"Cholesterol metabolism in cholestatic liver disease and liver transplantation: From molecular mechanisms to clinical implications","volume":"8","author":"Nemes","year":"2016","journal-title":"World J. Hepatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3354\/dao052261","article-title":"Spring viremia of carp (SVC)","volume":"52","author":"Ahne","year":"2002","journal-title":"Dis. Aquat. Organ."},{"key":"ref_28","first-page":"5","article-title":"Micorcystins: A Brief Overview of their Toxicity and Effects, with Special Reference to fish, Wildlife and Livestock","volume":"2009","author":"Butler","year":"2009","journal-title":"Calif. Environ. Prot. Agency"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.1897\/07-213.1","article-title":"Microcystin kinetics (bioaccumulation and elimination) and biochemical responses in common carp (Cyprinus carpio) and silver carp (Hypophthalmichthys molitrix) exposed to toxic cyanobacterial blooms","volume":"26","author":"Kopp","year":"2007","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1007\/s00204-015-1544-3","article-title":"Zebrafish Oatp-mediated transport of microcystin congeners","volume":"90","author":"Steiner","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.taap.2010.02.006","article-title":"The role of organic anion transporting polypeptides (OATPs\/SLCOs) in the toxicity of different microcystin congeners in vitro: A comparison of primary human hepatocytes and OATP-transfected HEK293 cells","volume":"245","author":"Fischer","year":"2010","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0304-4165(98)00107-X","article-title":"Identification of an enzymatically formed glutathione conjugate of the cyanobacterial hepatotoxin microcystin-LR: The first step of detoxication","volume":"1425","author":"Pflugmacher","year":"1998","journal-title":"Biochim. Biophys. Acta-Gen. Subj."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.aquatox.2012.03.004","article-title":"Quantitatively evaluating detoxification of the hepatotoxic microcystins through the glutathione and cysteine pathway in the cyanobacteria-eating bighead carp","volume":"116\u2013117","author":"He","year":"2012","journal-title":"Aquat. Toxicol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.aquatox.2013.12.001","article-title":"Rapid conversion and reversible conjugation of glutathione detoxification of microcystins in bighead carp (Aristichthys nobilis)","volume":"147","author":"Li","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.envint.2009.10.010","article-title":"Oxidative stress generation by microcystins in aquatic animals: Why and how","volume":"36","author":"Amado","year":"2010","journal-title":"Environ. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/jb\/mvs006","article-title":"Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species","volume":"151","author":"Miki","year":"2012","journal-title":"J. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Vermeij, W.P., and Backendorf, C. (2013). Reactive Oxygen Species (ROS) Protection via Cysteine Oxidation in the Epidermal Cornified Cell Envelope. Epidermal Cells, Springer.","DOI":"10.1007\/7651_2013_51"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"249","DOI":"10.3109\/08830185.2012.755176","article-title":"Reactive Oxygen Species in the Immune System","volume":"32","author":"Yang","year":"2013","journal-title":"Int. Rev. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.2298\/JSC100113106B","article-title":"Temporal and spatial variability of cyanobacterial toxins microcystins in three interconnected freshwater reservoirs","volume":"75","author":"Kohoutek","year":"2010","journal-title":"J. Serbian Chem. Soc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.3389\/fimmu.2017.01340","article-title":"Intramuscular DNA Vaccination of Juvenile Carp against Spring Viremia of Carp Virus Induces Full Protection and Establishes a Virus-Specific B and T Cell Response","volume":"8","author":"Embregts","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/j.molimm.2007.10.010","article-title":"Transcription of signal-3 cytokines, IL-12 and IFN\u03b1\u03b2, coincides with the timing of CD8\u03b1\u03b2 up-regulation during viral infection of common carp (Cyprinus carpio L.)","volume":"45","author":"Forlenza","year":"2008","journal-title":"Mol. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3354\/dao055229","article-title":"Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR","volume":"55","author":"Vesely","year":"2003","journal-title":"Dis. Aquat. Organ."},{"key":"ref_43","first-page":"38","article-title":"Methods of haematological examination of fish","volume":"122","author":"Pravda","year":"2012","journal-title":"Edice Metod."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"IFCC Expert Panel on Enzymes (1976). Provisional Recommendations on IFCC Methods for the Measurement of Catalytic Con-centrations of Enzymes. CIin. Chim. Acta, 70, F19\u2013F42.","DOI":"10.1016\/0009-8981(76)90437-X"},{"key":"ref_45","first-page":"371","article-title":"Proposition of standard method for catalytic concentration of lactate dehydrogenase in plasma diagnosis","volume":"17","author":"Hajzer","year":"1988","journal-title":"Biochem. Clin. Bohemoslov."},{"key":"ref_46","first-page":"1023","article-title":"Progress in the development of a recommended method for alkaline phosphatase activity measurements","volume":"26","author":"Tiez","year":"1980","journal-title":"Clin. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1093\/clinchem\/27.10.1642","article-title":"A candidate reference method for determination of total protein in serum. I. Development and validation","volume":"27","author":"Doumas","year":"1981","journal-title":"Clin. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0009-8981(71)90365-2","article-title":"Albumin standards and the measurement of serum albumin with bromcresol green","volume":"31","author":"Doumas","year":"1971","journal-title":"Clin. Chim. Acta"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1039\/an9729700142","article-title":"An improved colour reagent for the determination of blood glucose by the oxidase system","volume":"97","author":"Barham","year":"1972","journal-title":"Analyst"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/S0003-2670(01)80088-X","article-title":"Photometric determination of micro amounts of calcium with arsenazo III","volume":"53","author":"Michaylova","year":"1971","journal-title":"Anal. Chim. Acta"},{"key":"ref_51","first-page":"65","article-title":"Assignment of organic phosphorus in biological matter","volume":"6","author":"Kratochvila","year":"1977","journal-title":"Biochem. Clin. Bohemoslov."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1093\/clinchem\/27.9.1619a","article-title":"Novel chromogen for serum iron determinations","volume":"27","author":"Higgins","year":"1981","journal-title":"Clin. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/0003-2697(67)90312-0","article-title":"An enzymic and fluorophotometric method for estimating urea concentrations in nanoliter specimens","volume":"21","year":"1967","journal-title":"Anal. Biochem."},{"key":"ref_54","first-page":"403","article-title":"Enzymatic determination of total cholesterol in serum","volume":"12","author":"Roschlau","year":"1974","journal-title":"J. Clin. Chem. Clin. Biochem."},{"key":"ref_55","first-page":"391","article-title":"Ueber den Niederschlag, welchen Pikrins\u00e4ure in normalem Harn erzeugt und \u00fcber eine neue Reaction des Kreatinins","volume":"10","author":"Jaffe","year":"1886","journal-title":"Z Physiol.Chem."},{"key":"ref_56","first-page":"323","article-title":"Determination of phagocyte activity in whole blood of carp (Cyprinus carpio) by luminol-enhanced chemiluminescence","volume":"41","author":"Kubala","year":"1996","journal-title":"Vet. Med. (Praha)"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.aquaculture.2011.05.013","article-title":"The seasonal changes in innate immunity of the common carp (Cyprinus carpio)","volume":"318","author":"Lojek","year":"2011","journal-title":"Aquaculture"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5267","DOI":"10.1007\/s00216-019-01904-0","article-title":"Stable-isotope dilution LC-MS\/MS method for quantitative determination of microcystin conjugates with cysteine and glutathione in biotic matrices","volume":"411","author":"Kohoutek","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1007\/s00216-010-3860-z","article-title":"LC-MS analyses of microcystins in fish tissues overestimate toxin levels\u2014Critical comparison with LC-MS\/MS","volume":"398","author":"Kohoutek","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"296","DOI":"10.17221\/5549-VETMED","article-title":"Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.)","volume":"51","author":"Vesely","year":"2006","journal-title":"Vet. Med. (Praha)"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0022-1759(79)90098-X","article-title":"Periodate or glutaraldyehyde for preparing peroxidase conjugates?","volume":"30","author":"Boorsma","year":"1979","journal-title":"J. Immunol. 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