{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:00:46Z","timestamp":1791576046845,"version":"4.3.4"},"reference-count":55,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2008\/00108-8 and 2010\/09002-8"],"award-info":[{"award-number":["2008\/00108-8 and 2010\/09002-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["308083\/2013-0, 309392\/2015-2, and 312096\/2018-6"],"award-info":[{"award-number":["308083\/2013-0, 309392\/2015-2, and 312096\/2018-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the role of different SVMPs domains in inducing the expression of adhesion molecules at the microcirculation of the cremaster muscle of mice. We used Jararhagin (Jar)\u2014a PIII SVMP with intense hemorrhagic activity, and Jar-C\u2014a Jar devoid of the catalytic domain, with no hemorrhagic activity, both isolated from B. jararaca venom and BnP-1\u2014a weakly hemorrhagic P1 SVMP from B. neuwiedi venom. Toxins (0.5 \u00b5g) or PBS (100 \u00b5L) were injected into the scrotum of mice, and 2, 4, or 24 h later, the protein and gene expression of CD54 and CD31 in the endothelium, and integrins (CD11a and CD11b), expressed in leukocytes were evaluated. Toxins induced significant increases in CD54, CD11a, and CD11b at the initial time and a time-related increase in CD31 expression. In conclusion, our results suggest that, despite differences in hemorrhagic activities and domain composition of the SVMPs used in this study, they behave similarly to the induction of expression of adhesion molecules that promote leukocyte recruitment.<\/jats:p>","DOI":"10.3390\/toxins13110803","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T20:46:47Z","timestamp":1637009207000},"page":"803","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from Bothrops Snakes"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7125-5380","authenticated-orcid":false,"given":"Bianca C.","family":"Zychar","sequence":"first","affiliation":[{"name":"Laboratory of Pathophysiology, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia B.","family":"Clissa","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eneas","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adilson S.","family":"Alves","sequence":"additional","affiliation":[{"name":"Department. of Physiology and Biophysics, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiani","family":"Baldo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, State University of Londrina, Paran\u00e1 86051-990, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana L.","family":"Faquim-Mauro","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8395-9150","authenticated-orcid":false,"given":"Lu\u00eds Roberto C.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology, Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17063","DOI":"10.1038\/nrdp.2017.63","article-title":"Snakebite envenoming","volume":"3","author":"Calvete","year":"2017","journal-title":"Nat. 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