{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T14:55:41Z","timestamp":1781189741794,"version":"3.54.1"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2015\/17178-2"],"award-info":[{"award-number":["2015\/17178-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2018\/13895-0"],"award-info":[{"award-number":["2018\/13895-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2019\/22748-3"],"award-info":[{"award-number":["2019\/22748-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2017-0617"],"award-info":[{"award-number":["PICT 2017-0617"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["20020170200154BA"],"award-info":[{"award-number":["20020170200154BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["20020170100600BA"],"award-info":[{"award-number":["20020170100600BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"National Scientific and Technical Research Council","doi-asserted-by":"publisher","award":["PUE 0041"],"award-info":[{"award-number":["PUE 0041"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Hemolytic Uremic Syndrome (HUS) associated with Shiga-toxigenic Escherichia coli (STEC) infections is the principal cause of acute renal injury in pediatric age groups. Shiga toxin type 2 (Stx2) has in vitro cytotoxic effects on kidney cells, including human glomerular endothelial (HGEC) and Vero cells. Neither a licensed vaccine nor effective therapy for HUS is available for humans. Recombinant antibodies against Stx2, produced in bacteria, appeared as the utmost tool to prevent HUS. Therefore, in this work, a recombinant FabF8:Stx2 was selected from a human Fab antibody library by phage display, characterized, and analyzed for its ability to neutralize the Stx activity from different STEC-Stx2 and Stx1\/Stx2 producing strains in a gold standard Vero cell assay, and the Stx2 cytotoxic effects on primary cultures of HGEC. This recombinant Fab showed a dissociation constant of 13.8 nM and a half maximum effective concentration (EC50) of 160 ng\/mL to Stx2. Additionally, FabF8:Stx2 neutralized, in different percentages, the cytotoxic effects of Stx2 and Stx1\/2 from different STEC strains on Vero cells. Moreover, it significantly prevented the deleterious effects of Stx2 in a dose-dependent manner (up to 83%) in HGEC and protected this cell up to 90% from apoptosis and necrosis. Therefore, this novel and simple anti-Stx2 biomolecule will allow further investigation as a new therapeutic option that could improve STEC and HUS patient outcomes.<\/jats:p>","DOI":"10.3390\/toxins13110825","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T03:12:40Z","timestamp":1638328360000},"page":"825","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Deleterious Effects of Shiga Toxin Type 2 Are Neutralized In Vitro by FabF8:Stx2 Recombinant Monoclonal Antibody"],"prefix":"10.3390","volume":"13","author":[{"given":"Daniela","family":"Luz","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando D.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatogenia, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO Houssay-CONICET), Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00edssa L.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna S.","family":"Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1689-3213","authenticated-orcid":false,"given":"Beatriz E. C.","family":"Guth","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de S\u0101o Paulo, Sao Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3382-8184","authenticated-orcid":false,"given":"Wagner","family":"Quintilio","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biof\u00e1rmacos, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0650-7764","authenticated-orcid":false,"given":"Ana Maria","family":"Moro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biof\u00e1rmacos, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agostina","family":"Presta","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatogenia, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO Houssay-CONICET), Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Sacerdoti","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatogenia, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO Houssay-CONICET), Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Ibarra","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatogenia, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO Houssay-CONICET), Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[{"name":"Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, OT M5S 3E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachdev S.","family":"Sidhu","sequence":"additional","affiliation":[{"name":"Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, OT M5S 3E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Marta","family":"Amaral","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatogenia, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO Houssay-CONICET), Departamento de Fisiolog\u00eda, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0068-8506","authenticated-orcid":false,"given":"Roxane M. F.","family":"Piazza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia, Instituto Butantan, Sao Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.3201\/eid1104.040739","article-title":"Epidemiology of Escherichia coli O157:H7 Outbreaks, United States, 1982\u20132002","volume":"11","author":"Rangel","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1056\/NEJMoa1106483","article-title":"Epidemic Profile of Shiga-Toxin\u2013Producing Escherichia coli O104:H4 Outbreak in Germany","volume":"365","author":"Frank","year":"2011","journal-title":"N. Engl. J. 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