{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:03:36Z","timestamp":1790096616381,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2017\/07988-2"],"award-info":[{"award-number":["2017\/07988-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2019\/02298-3"],"award-info":[{"award-number":["2019\/02298-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2017\/18422-0"],"award-info":[{"award-number":["2017\/18422-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>The social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB\/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-\u03baB subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1\u03b2 production in non-activated macrophages and a reduction in the production of TNF-\u03b1 and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-\u03baB subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation.<\/jats:p>","DOI":"10.3390\/toxins13120850","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T03:12:56Z","timestamp":1638328376000},"page":"850","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biological and Inflammatory Effects of Antigen 5 from Polybia paulista (Hymenoptera, Vespidae) Venom in Mouse Intraperitoneal Macrophages"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-8661","authenticated-orcid":false,"given":"Murilo Luiz","family":"Bazon","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Artr\u00f3podes\u2014LBMA, Instituto de Biologia\u2014IB, Universidade Estadual Paulista C\u00e2mpus Rio Claro\u2014UNESP-RC, Avenida 24-A, n\u00b0 1515, Bela Vista, Rio Claro CEP 13506-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0771-1027","authenticated-orcid":false,"given":"Luis Gustavo Romani","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia Translacional\u2014LIT, Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Ci\u00eancias M\u00e9dicas\u2014FCM, Universidade Estadual de Campinas\u2014UNICAMP, Rua Tess\u00e1lia Vieira de Camargo, n\u00b0 126, Cidade Universit\u00e1ria \u201cZeferino Vaz\u201d, Campinas CEP 13083-887, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela Oliveira Sandrini","family":"Assugeni","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Artr\u00f3podes\u2014LBMA, Instituto de Biologia\u2014IB, Universidade Estadual Paulista C\u00e2mpus Rio Claro\u2014UNESP-RC, Avenida 24-A, n\u00b0 1515, Bela Vista, Rio Claro CEP 13506-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Machado","family":"Pinto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Artr\u00f3podes\u2014LBMA, Instituto de Biologia\u2014IB, Universidade Estadual Paulista C\u00e2mpus Rio Claro\u2014UNESP-RC, Avenida 24-A, n\u00b0 1515, Bela Vista, Rio Claro CEP 13506-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6951-5040","authenticated-orcid":false,"given":"Patr\u00edcia Ucelli","family":"Simioni","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina, Faculdade de Americana\u2014FAM, Avenida Joaquim B\u00f4er, n\u00b0 733, Jardim Luciane, Americana CEP 13477-360, SP, Brazil"},{"name":"Faculdade de Medicina, Universidade Anhembi Morumbi, Av. Rio das Pedras, 1601-Pomp\u00e9ia, Piracicaba CEP 13425-380, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-2841","authenticated-orcid":false,"given":"Ricardo de Lima","family":"Zollner","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunologia Translacional\u2014LIT, Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Ci\u00eancias M\u00e9dicas\u2014FCM, Universidade Estadual de Campinas\u2014UNICAMP, Rua Tess\u00e1lia Vieira de Camargo, n\u00b0 126, Cidade Universit\u00e1ria \u201cZeferino Vaz\u201d, Campinas CEP 13083-887, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Regina Brochetto","family":"Braga","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Artr\u00f3podes\u2014LBMA, Instituto de Biologia\u2014IB, Universidade Estadual Paulista C\u00e2mpus Rio Claro\u2014UNESP-RC, Avenida 24-A, n\u00b0 1515, Bela Vista, Rio Claro CEP 13506-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bazon, M.L., Perez-Riverol, A., Dos Santos-Pinto, J.R.A., Fernandes, L.G.R., Lasa, A.M., Justo-Jacomini, D.L., Palma, M.S., De Lima Zollner, R., and Brochetto-Braga, M.R. (2017). Heterologous expression, purification and immunoreactivity of the antigen 5 from polybia paulista wasp venom. Toxins, 9.","DOI":"10.3390\/toxins9090259"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1002\/pmic.201000414","article-title":"Proteomic characterization of the multiple forms of the PLAs from the venom of the social wasp Polybia paulista","volume":"11","author":"Santos","year":"2011","journal-title":"Proteomics"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.toxicon.2012.12.019","article-title":"Hyaluronidase from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): Cloning, structural modeling, purification, and immunological analysis","volume":"64","author":"Giratto","year":"2013","journal-title":"Toxicon"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1021\/pr4008927","article-title":"Using proteomic strategies for sequencing and post-translational modifications assignment of antigen-5, a major allergen from the venom of the social wasp polybia paulista","volume":"13","author":"Castro","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1210\/er.2008-0032","article-title":"The CAP superfamily: Cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins - Roles in reproduction, cancer, and immune defense","volume":"29","author":"Gibbs","year":"2008","journal-title":"Endocr. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/all.13000","article-title":"Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics","volume":"72","author":"Schiener","year":"2017","journal-title":"Allergy Eur. J. Allergy Clin. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Blank, S., Bazon, M.L., Grosch, J., Schmidt-Weber, C.B., Brochetto-Braga, M.R., Bil\u00f2, M.B., and Jakob, T. (2020). Antigen 5 Allergens of Hymenoptera Venoms and Their Role in Diagnosis and Therapy of Venom Allergy. Curr. Allergy Asthma Rep., 20.","DOI":"10.1007\/s11882-020-00954-0"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40409-015-0042-7","article-title":"Arthropod venom Hyaluronidases: Biochemical properties and potential applications in medicine and biotechnology","volume":"21","author":"Bordon","year":"2015","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2020.579409","article-title":"Precision Medicine in Hymenoptera Venom Allergy: Diagnostics, Biomarkers, and Therapy of Different Endotypes and Phenotypes","volume":"11","author":"Blank","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_10","first-page":"1","article-title":"Macrophage cytokines: Involvement in immunity and infectious diseases","volume":"5","author":"Duque","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/j.1398-9995.2012.02847.x","article-title":"IgE to recombinant allergens Api m 1, Ves v 1, and Ves v 5 distinguish double sensitization from crossreaction in venom allergy","volume":"67","author":"Hausmann","year":"2012","journal-title":"Allergy Eur. J. Allergy Clin. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.pep.2010.03.029","article-title":"Molecular cloning and expression in Pichia pastoris of a hypoallergenic antigen 5","volume":"73","author":"Pirpignani","year":"2010","journal-title":"Protein Expr. Purif."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6057","DOI":"10.4049\/jimmunol.166.10.6057","article-title":"Recombinant Allergens with Reduced Allergenicity but Retaining Immunogenicity of the Natural Allergens: Hybrids of Yellow Jacket and Paper Wasp Venom Allergen Antigen 5s","volume":"166","author":"King","year":"2001","journal-title":"J. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1016\/j.jaci.2017.12.990","article-title":"Venoms of Neotropical wasps lack cross-reactive carbohydrate determinants enabling reliable protein-based specific IgE determination","volume":"141","author":"Miehe","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0090-6980(99)00023-4","article-title":"Modulation of inducible nitric oxide synthase induction by prostaglandin E2 in macrophages: Distinct susceptibility in murine J774 and RAW 264.7 macrophages","volume":"58","author":"Lina","year":"1999","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1046\/j.1440-1711.1999.00847.x","article-title":"Stimulation of TNF-\u03b1, IL-1\u03b2 and nitrite release from mouse cultured spleen cells and lavaged peritoneal cells by mastoparan M","volume":"77","author":"Wu","year":"1999","journal-title":"Immunol. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jep.2005.02.026","article-title":"Il Effects of bee venom on the pro-inflammatory responses in RAW264.7 macrophage cell line","volume":"99","author":"Jang","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.jep.2006.11.008","article-title":"Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-\u03b1 production stimulated by LPS","volume":"111","author":"Han","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Saba, E., Shafeeq, T., Irfan, M., Lee, Y.Y., Kwon, H.W., Seo, M.G., Park, S.J., Lee, K.Y., and Rhee, M.H. (2017). Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say. Mediators Inflamm., 2017.","DOI":"10.1155\/2017\/6978194"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s00011-014-0738-0","article-title":"Vespa tropica venom suppresses lipopolysaccharide-mediated secretion of pro-inflammatory cyto-chemokines by abrogating nuclear factor-\u03ba B activation in microglia","volume":"63","author":"Kaushik","year":"2014","journal-title":"Inflamm. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1089\/jir.2008.0027","article-title":"Monocyte chemoattractant protein-1 (MCP-1): An overview","volume":"29","author":"Deshmane","year":"2009","journal-title":"J. Interf. Cytokine Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2019.02759","article-title":"More Than Just Attractive: How CCL2 Influences Myeloid Cell Behavior Beyond Chemotaxis","volume":"10","author":"Gschwandtner","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1365-2249.2006.03261.x","article-title":"Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1\u03b2 generation","volume":"147","author":"Nielsen","year":"2007","journal-title":"Clin. Exp. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1615\/CritRevEukarGeneExpr.v20.i2.10","article-title":"Tumor necrosis factor-\u03b1 signaling in macrophages","volume":"20","author":"Parameswaran","year":"2010","journal-title":"Crit. Rev. \u2122 Eukaryot. Gene Expr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1172\/JCI119488","article-title":"Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein","volume":"99","author":"Gabay","year":"1997","journal-title":"J. Clin. Invest."},{"key":"ref_26","first-page":"683","article-title":"I Nterleukin -10 and the I Nterleukin -10","volume":"1","author":"Moore","year":"2001","journal-title":"Mol. Cell. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1159\/000327839","article-title":"The honeybee antimicrobial peptide apidaecin differentially immunomodulates human macrophages, monocytes and dendritic cells","volume":"3","author":"Tavano","year":"2011","journal-title":"J. Innate Immun."},{"key":"ref_28","first-page":"57","article-title":"Kinetics of the phenotype and function of murine peritoneal macrophages following acute inflammation","volume":"1","author":"Wu","year":"2004","journal-title":"Cell. Mol. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.tibs.2004.11.009","article-title":"Phosphorylation of NF-\u03baB and I\u03baB proteins: Implications in cancer and inflammation","volume":"30","author":"Viatour","year":"2005","journal-title":"Trends Biochem. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1126\/science.281.5383.1680","article-title":"NF-\u2243B antiapoptosis: Induction of TRAF1 and TRAF2 and c-IAP1 and c- IAP2 to suppress caspase-8 activation","volume":"281","author":"Wang","year":"1998","journal-title":"Science"},{"key":"ref_31","first-page":"435","article-title":"Molecular control of immune\/inflammatory responses: Interactions between nuclear factor-\u03baB and steroid receptor-signaling pathways","volume":"20","author":"McKay","year":"1999","journal-title":"Endocr. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1124\/mi.2.1.22","article-title":"Inhibition of nuclear factor kappa B (NF-B): An emerging theme in anti-inflammatory therapies","volume":"2","author":"May","year":"2002","journal-title":"Mol. Interv."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1126\/science.7878466","article-title":"Control of I\u03baB-\u03b1 proteolysis by site-specific, signal-induced phosphorylation","volume":"267","author":"Brown","year":"1995","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.4049\/jimmunol.168.3.1441","article-title":"In Vivo Imaging of NF-\u03baB Activity","volume":"168","author":"Carlsen","year":"2002","journal-title":"J. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2015.00212","article-title":"Anatomy of a discovery: M1 and M2 macrophages","volume":"6","author":"Mills","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"355","DOI":"10.13102\/sociobiology.v60i4.355-361","article-title":"Molecular cloning, characterization and expression analysis of calreticulin gene in the ant polyrhachis vicina roger (hymenoptera: SsFormicidae)","volume":"60","author":"Liu","year":"2013","journal-title":"Sociobiology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/nri2448","article-title":"Exploring the full spectrum of macrophage activation","volume":"8","author":"Mosser","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/0003-2697(77)90428-6","article-title":"A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250","volume":"79","author":"Sedmak","year":"1977","journal-title":"Anal. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12906-015-0896-9","article-title":"Evaluation of in vitro anti-inflammatory effects of crude ginger and rosemary extracts obtained through supercritical CO2 extraction on macrophage and tumor cell line: The influence of vehicle type","volume":"15","author":"Justo","year":"2015","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids","volume":"126","author":"Green","year":"1982","journal-title":"Anal. Biochem."}],"container-title":["Toxins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6651\/13\/12\/850\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:37:08Z","timestamp":1760168228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6651\/13\/12\/850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,29]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["toxins13120850"],"URL":"https:\/\/doi.org\/10.3390\/toxins13120850","relation":{},"ISSN":["2072-6651"],"issn-type":[{"value":"2072-6651","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,29]]}}}