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Ectatomma opaciventre is a previously unrecorded solitary hunting ant from the Brazilian Cerrado. To overcome this hindrance, the present study performed the in vitro enzymatic, biochemical, and biological activities of E. opaciventre to better understand the properties of this venom. Its venom showed several proteins with masses ranging from 1\u2013116 kDa, highlighting the complexity of this venom. Compounds with high enzymatic activity were described, elucidating different enzyme classes present in the venom, with the presence of the first L-amino acid oxidase in Hymenoptera venoms being reported. Its crude venom contributes to a state of blood incoagulability, acting on primary hemostasis, inhibiting collagen-induced platelet aggregation, and operating on the fibrinolysis of loose red clots. Furthermore, the E. opaciventre venom preferentially induced cytotoxic effects on lung cancer cell lines and three different species of Leishmania. These data shed a comprehensive portrait of enzymatic components, biochemical and biological effects in vitro, opening perspectives for bio-pharmacological application of E. opaciventre venom molecules.<\/jats:p>","DOI":"10.3390\/toxins14010037","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T23:35:09Z","timestamp":1641771309000},"page":"37","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2827-8509","authenticated-orcid":false,"given":"Lucas Ian Veloso","family":"Correia","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"},{"name":"Laboratory of Nanobiotechnology Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Van Petten de Vasconcelos","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"},{"name":"Laboratory of Nanobiotechnology Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1453-3185","authenticated-orcid":false,"given":"Fernanda Gobbi","family":"Amorim","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Toxins, Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto 14040-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1951-6205","authenticated-orcid":false,"given":"Sarah Natalie","family":"Cirilo Gimenes","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"},{"name":"Laboratory of Immunopathology, Institute Butantan, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Polloni","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Alves Pereira","family":"Zoia","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f4nica Soares","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0292-1052","authenticated-orcid":false,"given":"J\u00e9ssica Peixoto","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly A. Geraldo","family":"Yoneyama","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6031-9193","authenticated-orcid":false,"given":"Jean Carlos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Ecology, Institute of Biology, Federal University of Sergipe, S\u00e3o Cristov\u00e3o 49060-108, SE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6712-6033","authenticated-orcid":false,"given":"Eliane Candiani","family":"Arantes","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Toxins, Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto 14040-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veridiana de Melo","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Ricardo","family":"Goulart","sequence":"additional","affiliation":[{"name":"Laboratory of Nanobiotechnology Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Santos","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18172","DOI":"10.1073\/pnas.0605858103","article-title":"Evaluating alternative hypotheses for the early evolution and diversification of ants","volume":"103","author":"Brady","year":"2006","journal-title":"Proc. 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