{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:39:04Z","timestamp":1775198344433,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China-Shandong Joint Fund","award":["U2106205"],"award-info":[{"award-number":["U2106205"]}]},{"name":"National Natural Science Foundation of China-Shandong Joint Fund","award":["41676093"],"award-info":[{"award-number":["41676093"]}]},{"name":"National Natural Science Foundation of China","award":["U2106205"],"award-info":[{"award-number":["U2106205"]}]},{"name":"National Natural Science Foundation of China","award":["41676093"],"award-info":[{"award-number":["41676093"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Neurotoxin \u03b2-N-methylamino-L-alanine (BMAA) is hypothesized as an important pathogenic factor for neurodegenerative diseases such as amyotrophic lateral sclerosis\/parkinsonism-dementia complex (ALS-PDC). Comparative study on the accuracy of BMAA analyzed by the regular LC-MS\/MS methods is still limited for different biological matrices. In this study, a free-BMAA sample of cyanobacterium and BMAA-containing positive samples of diatom, mussel, scallop, and oyster were extracted with varied extraction ratios (ER) ranging from 1:20 to 1:2000. These extracts were then purified by MCX cartridges. After SPE purification, these different biological samples were analyzed by two common LC-MS\/MS analysis methods, a direct analysis without derivatization by a hydrophilic interaction liquid chromatography (HILIC)-MS\/MS and pre-column 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization combined with a C18 column. The results suggested that the recoveries of BMAA spiked in the cyanobacterial sample were close to 100% in the total soluble form extracts with the ER of 1:100 (g\/mL) and the precipitated bound form extracts with the ER of 1:500. The recommended ER for the precipitated bound form of BMAA in diatoms and the total soluble form of BMAA in mollusks are 1:500 and 1:50, respectively. The quantitative results determined by the AQC derivatization method were lower than those determined by the direct analysis of the HILIC method in diatom and mollusk samples. The results of the HILIC method without the derivatization process were closer to the true value of BMAA in cyanobacteria. This work contributes to the performance of the solid-phase extraction (SPE) purification protocol and the accuracy of BMAA analysis by LC-MS\/MS in diverse biological samples.<\/jats:p>","DOI":"10.3390\/toxins14060387","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T04:46:23Z","timestamp":1654145183000},"page":"387","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Matrix Effect of Diverse Biological Samples Extracted with Different Extraction Ratios on the Detection of \u03b2-N-Methylamino-L-Alanine by Two Common LC-MS\/MS Analysis Methods"],"prefix":"10.3390","volume":"14","author":[{"given":"Peng","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangbing","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"},{"name":"Key Laboratory of Marine Environment and Ecology, Ocean University of China, Ministry of Education, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4487-1249","authenticated-orcid":false,"given":"Aifeng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"},{"name":"Key Laboratory of Marine Environment and Ecology, Ocean University of China, Ministry of Education, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guowang","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108342","DOI":"10.1016\/j.exer.2020.108342","article-title":"Damaging Effects of BMAA on Retina Neurons and M\u00fcller Glial Cells","volume":"202","author":"Soto","year":"2020","journal-title":"Exp. 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