{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T10:20:48Z","timestamp":1773829248845,"version":"3.50.1"},"reference-count":89,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Ministerio de Ciencia y Tecnolog\u00eda (MinCiencias) of Co-lombia","doi-asserted-by":"publisher","award":["111577757673"],"award-info":[{"award-number":["111577757673"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad de Antioquia in Medell\u00edn, Colombia","award":["111577757673"],"award-info":[{"award-number":["111577757673"]}]},{"name":"Ministerio de Ciencia y Tecnolog\u00eda (MinCiencias) of Colombia","award":["111577757673"],"award-info":[{"award-number":["111577757673"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Spider venoms constitute a trove of novel peptides with biotechnological interest. Paucity of next-generation-sequencing (NGS) data generation has led to a description of less than 1% of these peptides. Increasing evidence supports the underestimation of the assembled genes a single transcriptome assembler can predict. Here, the transcriptome of the venom gland of the spider Pamphobeteus verdolaga was re-assembled, using three free access algorithms, Trinity, SOAPdenovo-Trans, and SPAdes, to obtain a more complete annotation. Assembler\u2019s performance was evaluated by contig number, N50, read representation on the assembly, and BUSCO\u2019s terms retrieval against the arthropod dataset. Out of all the assembled sequences with all software, 39.26% were common between the three assemblers, and 27.88% were uniquely assembled by Trinity, while 27.65% were uniquely assembled by SPAdes. The non-redundant merging of all three assemblies\u2019 output permitted the annotation of 9232 sequences, which was 23% more when compared to each software and 28% more when compared to the previous P. verdolaga annotation; moreover, the description of 65 novel theraphotoxins was possible. In the generation of data for non-model organisms, as well as in the search for novel peptides with biotechnological interest, it is highly recommended to employ at least two different transcriptome assemblers.<\/jats:p>","DOI":"10.3390\/toxins14060408","type":"journal-article","created":{"date-parts":[[2022,6,19]],"date-time":"2022-06-19T21:19:26Z","timestamp":1655673566000},"page":"408","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Improving the Annotation of the Venom Gland Transcriptome of Pamphobeteus verdolaga, Prospecting Novel Bioactive Peptides"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9320-0989","authenticated-orcid":false,"given":"Cristian","family":"Salinas-Restrepo","sequence":"first","affiliation":[{"name":"Grupo Toxinolog\u00eda, Alternativas Terap\u00e9uticas y Alimentarias, Facultad de Ciencias Farmac\u00e9uticas y Alimentarias, Universidad de Antioquia, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Misas","sequence":"additional","affiliation":[{"name":"Corporaci\u00f3n para Investigaciones Biol\u00f3gicas, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Estrada-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Grupo Toxinolog\u00eda, Alternativas Terap\u00e9uticas y Alimentarias, Facultad de Ciencias Farmac\u00e9uticas y Alimentarias, Universidad de Antioquia, Medell\u00edn 050012, Colombia"},{"name":"Centro de Investigaci\u00f3n en Recursos Naturales y Sustentabilidad, Universidad Bernardo O\u2019Higgins, Aven-ida Viel 1497, Santiago 7750000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7923-9158","authenticated-orcid":false,"given":"Juan Carlos","family":"Quintana-Castillo","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Cooperativa de Colombia, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7829-0568","authenticated-orcid":false,"given":"Fanny","family":"Guzman","sequence":"additional","affiliation":[{"name":"N\u00facleo Biotecnolog\u00eda Curauma (NBC), Pontif\u00edcia Universidad Cat\u00f3lica de Valpara\u00edso, Valpara\u00edso 2374631, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8695-6972","authenticated-orcid":false,"given":"Juan C.","family":"Calder\u00f3n","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3437-6308","authenticated-orcid":false,"given":"Marco A.","family":"Giraldo","sequence":"additional","affiliation":[{"name":"Biophysics Group, Institute of Physics, University of Antioquia, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9979-8416","authenticated-orcid":false,"given":"Cesar","family":"Segura","sequence":"additional","affiliation":[{"name":"Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medell\u00edn 050012, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"key":"ref_1","unstructured":"World Spider Catalog (2022, January 31). 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