{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:48:46Z","timestamp":1777520926919,"version":"3.51.4"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Training Program of Innovation and Entrepreneurship for Undergraduates","award":["202110635035"],"award-info":[{"award-number":["202110635035"]}]},{"name":"National Training Program of Innovation and Entrepreneurship for Undergraduates","award":["XDJK2020B044"],"award-info":[{"award-number":["XDJK2020B044"]}]},{"name":"National Training Program of Innovation and Entrepreneurship for Undergraduates","award":["32072137"],"award-info":[{"award-number":["32072137"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["202110635035"],"award-info":[{"award-number":["202110635035"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["XDJK2020B044"],"award-info":[{"award-number":["XDJK2020B044"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["32072137"],"award-info":[{"award-number":["32072137"]}]},{"name":"National Natural Science Foundation of China","award":["202110635035"],"award-info":[{"award-number":["202110635035"]}]},{"name":"National Natural Science Foundation of China","award":["XDJK2020B044"],"award-info":[{"award-number":["XDJK2020B044"]}]},{"name":"National Natural Science Foundation of China","award":["32072137"],"award-info":[{"award-number":["32072137"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>\u03b1-zearalenol (\u03b1-ZOL) is a mycotoxin with a strong estrogen effect that affects the synthesis and secretion of sex hormones and is transported to target organs through human serum albumin (HSA). Additionally, it has been reported that curcumin can also bind to HSA with high affinity at the same binding site as \u03b1-ZOL. Additionally, several studies reported that reducing the bound fraction of \u03b1-ZOL contributes to speeding up the elimination rate of \u03b1-ZOL to reduce its hazard to organs. Therefore, to explore the influence of a nutrition intervention with curcumin on \u03b1-ZOL effects, the competitive displacement of \u03b1-ZOL from HSA by curcumin was investigated using spectroscopic techniques, ultrafiltration techniques and HPLC methods. Results show that curcumin and \u03b1-ZOL share the same binding site (subdomain IIA) on HSA, and curcumin binds to HSA with a binding constant of 1.12 \u00d7 105 M\u22121, which is higher than that of \u03b1-ZOL (3.98 \u00d7 104 M\u22121). Ultrafiltration studies demonstrated that curcumin could displace \u03b1-ZOL from HSA to reduce \u03b1-ZOL\u2019s binding fraction. Synchronous fluorescence spectroscopy revealed that curcumin could reduce the hydrophobicity of the microenvironment of an HSA\u2013\u03b1-ZOL complex. This study is of great significance for applying curcumin and other highly active foodborne components to interfere with the toxicokinetics of \u03b1-ZOL and reduce its risk of its exposure.<\/jats:p>","DOI":"10.3390\/toxins14090604","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T03:55:38Z","timestamp":1662004538000},"page":"604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Study of Competitive Displacement of Curcumin on \u03b1-zearalenol Binding to Human Serum Albumin Complex Using Fluorescence Spectroscopy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2523-8392","authenticated-orcid":false,"given":"Yifang","family":"Li","sequence":"first","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3734-7126","authenticated-orcid":false,"given":"Hongyuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"},{"name":"Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China"},{"name":"Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"},{"name":"Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China"},{"name":"Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"},{"name":"Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China"},{"name":"Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"},{"name":"Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China"},{"name":"Key Laboratory of Luminescence Analysis and Molecular Sensing, Southwest University, Ministry of Education, Chongqing 400715, China"},{"name":"Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhu","family":"Liu","sequence":"additional","affiliation":[{"name":"Foshan Micro Miracles Biotechnology Company, Foshan 528000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Food Science, Southwest University, Chongqing 400715, China"},{"name":"Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China"},{"name":"Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China"},{"name":"Key Laboratory of Condiment Supervision Technology for State Market Regulation, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.toxicon.2010.06.020","article-title":"Effects of zearalenone and its derivatives on the innate immune response of swine","volume":"56","author":"Marin","year":"2010","journal-title":"Toxicon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fct.2006.07.030","article-title":"Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: An oestrogenic mycotoxin","volume":"45","author":"Zinedine","year":"2007","journal-title":"Food Chem. 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