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Peptides from scorpion venom present potent pharmacological actions on ionic conductance used to characterize the function of channels but can also be helpful to develop organic pharmacological tools. Using electrophysiological recording coupled with calcium measurement, we tested the potent effect of peptides extracted from Leuirus quinquestratus quinquestratus venom on the calcium-activated chloride current expressed in smooth muscle cells freshly dissociated from rat portal veins. We identified one peptide which selectively inhibited the chloride conductance without effects on either calcium signaling or calcium and potassium currents expressed in this cell type. The synthetic peptide had the same affinity, but the chemical modification of the amino acid sequence altered the efficiency to inhibit the calcium-activated chloride conductance.<\/jats:p>","DOI":"10.3390\/toxins14110780","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T21:33:02Z","timestamp":1668115982000},"page":"780","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of a Family of Scorpion Toxins Modulating Ca2+-Activated Cl\u2212 Current in Vascular Myocytes"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8079-6851","authenticated-orcid":false,"given":"Jean-Luc","family":"Morel","sequence":"first","affiliation":[{"name":"Univ. Bordeaux, CNRS, INCIA, UMR 5287, EPHE, F33000 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Mokrzycki","sequence":"additional","affiliation":[{"name":"Univ. Lille, Institut Pasteur de Lille, F59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Lippens","sequence":"additional","affiliation":[{"name":"Toulouse Biotechnology Institute (TBI), Universit\u00e9 de Toulouse, CNRS, INRAE, INSA, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Drobecq","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, UMR 9017, INSERM U1019, CHRU Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille, F59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Sauti\u00e8re","sequence":"additional","affiliation":[{"name":"Independent Researcher, F59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Hugues","sequence":"additional","affiliation":[{"name":"Independent Researcher, F33000 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.3389\/fcell.2021.702317","article-title":"Cell Death Induction and Protection by Activation of Ubiquitously Expressed Anion\/Cation Channels. 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