{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:33:16Z","timestamp":1793277196949,"version":"4.5.0"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T00:00:00Z","timestamp":1674950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32160599"],"award-info":[{"award-number":["32160599"]}]},{"name":"National Natural Science Foundation of China","award":["32160598"],"award-info":[{"award-number":["32160598"]}]},{"name":"National Natural Science Foundation of China","award":["32172296"],"award-info":[{"award-number":["32172296"]}]},{"name":"National Natural Science Foundation of China","award":["20212ACB205011"],"award-info":[{"award-number":["20212ACB205011"]}]},{"name":"National Natural Science Foundation of China","award":["20202ACB215004"],"award-info":[{"award-number":["20202ACB215004"]}]},{"name":"National Natural Science Foundation of China","award":["20212BAB205032"],"award-info":[{"award-number":["20212BAB205032"]}]},{"name":"National Natural Science Foundation of China","award":["20192BAB204021"],"award-info":[{"award-number":["20192BAB204021"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["32160599"],"award-info":[{"award-number":["32160599"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["32160598"],"award-info":[{"award-number":["32160598"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["32172296"],"award-info":[{"award-number":["32172296"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["20212ACB205011"],"award-info":[{"award-number":["20212ACB205011"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["20202ACB215004"],"award-info":[{"award-number":["20202ACB215004"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["20212BAB205032"],"award-info":[{"award-number":["20212BAB205032"]}]},{"name":"Jiangxi Provincial Natural Science Foundation","award":["20192BAB204021"],"award-info":[{"award-number":["20192BAB204021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Staphylococcal enterotoxin A (SEA) has presented enormous difficulties in dairy food safety and the sensitive detection of SEA provides opportunities for effective food safety controls and staphylococcal food poisoning tracebacks. Herein, a novel aggregation-induced emission (AIE)-based sandwich lateral flow immunoassay (LFIA) was introduced to detect SEA by using red-emissive AIE nanoparticles (AIENPs) as the fluorescent nanoprobe. The nanoprobe was constructed by directly immobilising antibodies on boronate-tagged AIENPs (PBA-AIENPs) via a boronate affinity reaction, which exhibited a high SEA-specific affinity and remarkable fluorescent performance. Under optimal conditions, the ultrasensitive detection of SEA in pasteurised milk was achieved within 20 min with a limit of detection of 0.04 ng mL\u22121. The average recoveries of the PBA-AIENP-LFIA ranged from 91.3% to 117.6% and the coefficient of variation was below 15%. It was also demonstrated that the PBA-AIENP-LFIA had an excellent selectivity against other SE serotypes. Taking advantage of the excellent sensitivity of this approach, real chicken and salad samples were further analysed, with a high versatility and accuracy. The proposed PBA-AIENP-LFIA platform shows promise as a potent tool for the identification of additional compounds in food samples as well as an ideal test method for on-site detections.<\/jats:p>","DOI":"10.3390\/toxins15020113","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T01:33:37Z","timestamp":1675042417000},"page":"113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Aggregation-Induced Red Emission Nanoparticle-Based Lateral Flow Immunoassay for Highly Sensitive Detection of Staphylococcal Enterotoxin A"],"prefix":"10.3390","volume":"15","author":[{"given":"Hanpeng","family":"Xiong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xirui","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanang","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8908-0443","authenticated-orcid":false,"given":"Xiaolin","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"},{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-0841","authenticated-orcid":false,"given":"Yonghua","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China"},{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China"},{"name":"State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.3390\/toxins2071751","article-title":"Food Poisoning and Staphylococcus aureus Enterotoxins","volume":"2","author":"Argudin","year":"2010","journal-title":"Toxins"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.3390\/toxins2082177","article-title":"Staphylococcal enterotoxins","volume":"2","author":"Pinchuk","year":"2010","journal-title":"Toxins"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1128\/jcm.23.6.1138-1139.1986","article-title":"Involvement of staphylococcal enterotoxins in nonmenstrual toxic shock syndrome","volume":"23","author":"Crass","year":"1986","journal-title":"J. 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